Competitors
Competitors: ELISA
• Focus Diagnostics Dengue Fever Virus IgM Capture DxSelectTM
• Focus Diagnostics Dengue Fever Virus IgG DxSelectTM
Focus Diagnostics Dengue Fever
Virus IgM Capture DxSelectTM
• 3 hr 45 minutes incubation time (over 1 hour longer
than Panbio)
• 7 steps required (Panbio has 6 steps)
• Has been compared to Panbio Dengue Duo IgG & IgM
Capture ELISA (Cuzzubbo et al., 1999, Clin. Diagn.
Lab. Immunol., 6(6):705-712.)
– Overall performance of the Panbio Dengue Duo was higher
than the MRL/Focus Diagnostics ELISA.
– MRL/Focus Diagnostics IgM ELISA has a lower cut-off than
the Duo and consequently a higher sensitivity (94% vs 89%)
Focus Diagnostics Dengue Fever
Virus IgM Capture DxSelectTM
• Focus ELISA is less specific in infections other than
dengue than the Panbio kits.
– Panbio Dengue Duo 79% specificity in JE and 100%
specificity in non-flavivirus infections
– Focus/MRL 25% specificity in JE & 91% in non-flavivirus
infections.
Focus Diagnostics Dengue Fever
Virus IgG DxSelectTM
• 1 hr 45 minutes incubation time (Panbio only 1 hr 10
min)
• 6 steps required (Panbio only has 5 steps)
Competitors: Rapid test
Manufacturer
Description
Performance
UniTest
(Unison
Collaborative,
SingaporeMalaysia)
Rapid strip
IgM & IgG line
Diff. 1° & 2°
30 mins
Serum, plasma, whole blood
Pack insert exactly the same
as Merlin Labs – OEM from
Merlin?
Merlin Dengue
Fever IgG & IgM
Combo Test (Merlin
Labs, USA)
Rapid strip
IgM & IgG line
Diff. 1° & 2°
30 mins
Serum, plasma, whole blood
12 month exp, RT storage
Inhouse study (MS)
- 0% specificity for IgM (20/20
Aust blood donors came up
positive)
- Some strips fell apart or did
not work (sample did not flow
up strip)
Competitors: Rapid test cont.
Manufacturer
Description
Performance
Core Dengue IgGIgM (UK)
Rapid Cassette, 2 wells
IgM & IgG line
No 1°/2° differentiation
claimed
15 minutes
Serum, plasma, whole blood
24 month exp, 4 – 30°C
Performance seemed OK
based on small inhouse study
(MG) (9/10 active dengue
correct; 4/5 negs correct)
Bias Dengue
IgM/IgG
(Millennium
Biotechnology)
Rapid cassette (Bidirectional)
IgM & IgG line
No 1°/2° differentiation
Serum & whole blood
24 months exp, 8 - 30°C
5 - 30 mins
Insensitive in detecting
primary infection (Ong inhouse
study 2004)
Poor primary sensitivity & high
background (MG Inhouse
study, 2004 –based on 10
samples)
Competitors: Rapid test cont.
Manufacturer
Description
Performance
Dengue Combo
Card Test
IgM & IgG line
Oversensitive for IgG and
under sensitive for IgM (Ong
inhouse study 2004)
Peerless
Rapid cassette (Bidirectional)
IgM & IgG line
15 – 20 mins
Serum/plasma/whole blood
Diff. 1° & 2°
SD Dengue
(Standard
Diagnostics)
Insensitive in detecting
primary infection (Ong inhouse
study 2004)
Competitors: Rapid test cont.
Manufacturer
Description
Performance
Dengucheck WB
IgM & IgG Pack
Insert (Zephyr
Biomedicals, Tulip
Group)
Rapid Cassette
IgM & IgG line
Diff. 1° & 2°
15 minutes
Serum, plasma, whole blood
2 wells
24 month exp, 4 – 30°C
Rec dengue envelope
proteins
Same product as the Core
test??