Industriell bruk og nytte av
enzymer
by
Kjartan Sandnes, Dr.
Daglig leder og forskningsleder
Biorefining salmon co-products
Enzymatic
hydrolysis
Oil
Water and
enzymes
Water soluble protein
Exact time
and temp
Sediment
Some key figures
• Norwegian sea areas 7
times the size of land area
• All included, Norway is
the 13th largest country
in the world
• Second largest seafood
export nation
• 53 bill. NOK
• 33 mill seafood meals on
the table every day
Norway 2012:
«Non-edible fish raw materials»
White fish (cod, haddock, saithe)
Herring
Aquaculture (salmon, rainbow trout)
Total co-products
325.000
239.000
350.000
914.000
«Industrial fish» (to the fish meal- and oil industry 2012)
T O T A L (not for direct human consumption)
202.000
1.116.000
NOTE! Seaweeds and shellfish not included
Source: fhf, Sildesalgslaget
Source: fhf, Sintef, Sildesalgslaget
Biomega AS –
a blue biorefinery
•
•
•
•
•
•
2000: Marine Bioproducts AS
established
2002: Production established with
patented continuous enzymatic
hydrolysis process. No chemical
processing aids required
2007: New site acquired for new
factory
2011: Six patent families filed so far
2011: 30.000 tons raw materials
processed . Turnover 107 mill NOK
2012: New factory with a capacity of
60.000 tons annually (130 mill NOK
inv.) - opened May 2012
6
Raw material transportation
to Biomega – heads and
backbones
Constant efforts to improve
quality (cooling systems) and
logistics
Raw material transportation to
Biomega – viscera in bulk by truck
Page 8
New processing plant
production from May 2012
9
Overview of new plant
10
Biorefining - current and next
generation products
MB
Process
Hydrolysate
Oil
Sediment
Bones
(soluble protein)
(fat)
((unsoluble protein)
(minerals, protein)
NEW AND INNOVATIVE PROCESSES
enzyme technology – extraction – separation - isolation
BIOACTIVE PEPTIDES
PEPTONES
FLAVOURS
MODIFIED LIPIDS
PHOSPHOSLIPIDS
POWDERS
EXTRACTS
E X A M P L E S
MARINE MINERALS
GLUCOSAMINES
CHONDROITIN
BIOACTIVE PEPTIDES
How enzymes can be used
Herring
Hydrolysates
Salmon Hydrolysates
HH 1
HH 2
MB
HR 1
MB
HR 2
MB
HR 3
0
0
2.5
0.2
0.0
30 000 – 20 000 daltons
1.3
0
0.7
0.2
0.0
20 000 – 10 000 daltons
1.2
0.2
12.6
7.1
4.6
10 000 – 5 000 daltons
12.4
5.2
17.8
15.5
10.0
5 000 - 1 000 daltons
14.6
10
13.3
9.7
10.2
1 000 -
53.1
66.1
38.3
40.5
33.9
17.4
18.4
14.7
26.7
41.3
2100
1000
4900
2800
1900
Test sample
Peptide size distribution (% of total detected)
> 30 000 daltons
100 daltons
< 100 daltons
Rough estimated average Mw
Carbon source
Nitrogen source
Micronutrients
Glucose,
Glycerol
Ammonium salts,
Nitrates,
Peptone (amino
acids/peptides)
Phosphates,
Sulphates,
Trace metals,
Vitamins
SEM
Typical growth medium
Fungi
Yeasts
Antibiotics
Bacteria
Enzymes
Vaccines
Mammalian cells
Other
Saccharomyces cerevisiae Growth Curves
6
LACH
SOYP
OXYE
5
CASA
GELP
SEM
OD600
4
BDYE
MBPT
“good for yeasts” from supplier 2
3
2
highest AN from supplier 1
1
highest AN from supplier 2
0
0
1
2
3
4
5
6
7
8
9
10
Time (h)
11
12
13
14
15
16
17
18
19
20
Mask writer
Thank you!