INFECTIOUS DISEASES ABDI SOLOMON(MD) Generally infectious diseases result from bacteria, viruses, fungi, and parasites. Infectious diseases remain a major cause of death and are responsible for worsening the living conditions of many millions of people Infectious diseases often do not occur in isolated cases Many factors affect the likelihood of acquiring infections which include, host, environment and microbial factors. Many factors affect the likelihood of acquiring infections which include host environment Infectious agents. Host factor Many specific host factors influence the likelihood of acquiring an infectious disease. Age, immunization history, prior illnesses, level of nutrition, pregnancy, coexisting illness, and perhaps emotional state all have some impact on the risk of infection after exposure to a potential pathogen. Defense-immunity-innate/adaptive Immune response: Is a defense mechanism developed by the host for recognizing and responding to microorganisms. two major classes. Innate and Acquired Immunity. Innate immunity (Natural Immunity) : Is first line of defense and serves to protect the host with out prior exposure to the infectious agent. is nonspecific and has no memory.eg phagocytoses by macrophages and nutrophils, complement system etc Acquired (Adaptive) Immunity: specific immune mechanism developed against a particular organism.takes long time to develop It has two major arms: .Cellular immunity: comprising T- lymphocytes, NK cells . Humeral Immunity : comprises of B-Lymphocytes and antibodies produced by plasma cells. Enviromental factors Factors such as geography (e.g. altitude and malaria), environment (e.g. mosquito breeding site and malaria), disease vectors Cleans of the environment Over crowding Infectious agent Viruses Viruses contain two types of macromolecule: proteins and nucleic acids, the latter of only one type (either DNA or RNA). Bacteria Long known as 'prokaryotes', bacteria share the following essential features: they contain both RNA and DNA, have facilities for protein metabolism and are generally free living. Eukaryotes These organisms exhibit subcellular compartmentalisation with intracellular functions being achieved by specific organelles, e.g. nucleus, chloroplasts, mitochondria and Golgi apparatus.Fungi,Protozoa,parasites Incubation period: The incubation period is the period between the invasion of the tissues by pathogens and the appearance of clinical features of infection. The period of infectivity is the time that the patient is infectious to others Typhoid (Enteric) Fever Definition: Typhoid fever is a systemic infection caused by dissemination of Salmonella typhi and occasionally by S. paratyphi A and B and S.typhimarium non capsulated, gram negative motile and rod shaped bacteria. Antigens: H-located in the cell capsule H (flagellar antigen). O- (Somatic or cell wall antigen). Vi -(polysaccharide virulence) “widel test” A schematic diagram of a single Salmonella typhi cell showing the locations of the H (flagellar), 0 (somatic), and Vi (K envelope) antigens. 11 Epidemiology Continues to be a global health problem Areas with a high incidence include Asia, Africa and Latin America Affects about 6000000 people with more than 600000 deaths a year. 80% in Asia Transmission fecal-oral route close contact with patients or carriers contaminated water and food flies and cockroaches. 13 Pathogenesis Ingested orally Stomach barrier (some Eliminated) enters the small intestine Penetrate the mucus layer enter mononuclear phagocytes of ileal peyer's patches and mesenteric lymph nodes proliferate in mononuclear phagocytes spread to blood. initial bacteremia (Incubation period). 14 PATHOGENESIS enter spleen, liver and bone marrow (reticuloendothelial system) further proliferation occurs A lot of bacteria enter blood again-(second bacteremia). Recovery 15 S.Typhi. 2nd bacteremia liver、spleen、gall、 BM ,ect early stage&acme stage (1-3W) stomach (mono Bac. In gall nuclea r phago cytes ) Bac. In feces Lower ileum 16 LN Proliferate,swell necrosis defervescence stage Enterorrhagia,i ntestinal perforation S.Typhi eliminated convalvescence stage (4-5w) peyer's patches & mesenteric lymph nodes (3-4w) thoracic duct 1st bacteremia (Incubation stage) 10-14d CLINICAL MANIFESTATION The incubation period varies from 3-60 days. First week Fever is high grade, with a daily increase in a step-ladder pattern for the 1st one week and then becomes persistent. Headache , malaise , Abdominal pain Initially diarrhea or loss stole followed by constipation in adults, diarrhea is dominate feature in children Relative bradycardia Splenomegally Hepatomegaly “Rose spots” 17 Second week Fever becomes continuous The patient becomes very ill and withdrawn confused, delirious and sometimes may be even comatose Third Week • The patient goes to a pattern of “typhoidal state" characterized by extreme toxemia, disorientation, and “pea-soup” diarrhea and sometimes may be complicated by intestinal perforation and hemorrhage. 18 Fourth Week Fever starts to decrease and the patient may deferveresce with resolution of symptoms. At this point patient may lose weight. Relapse may occur in 10% of cases. fatal complication intestinal hemorrhage(serious blding 2-8%) intestinal perforation (1-4%) severe toxemia 19 LABORATORY FINDINGS Routine examinations: White blood cell count is normal or decreased. Leukocytopenia 20 BACTERIOLOGICAL EXAMINATIONS: Blood culture -mostly (up to 90%) patients have positive culture in the 1stweek, and only 50% by the 3rdweek. The yield is much lower if patient has taken antibiotics prior to the test. Stool culture is negative in the first week and becomes positive in 75%of patients in the 3rdweek. Urine culture parallels stool culture. 21 WIDAL TEST "O" agglutinin antibody titer ≥1:80 and "H" ≥1:160 or "O" 4 times higher supports a diagnosis of typhoid fever. "O" rises alone, not "H", early of the disease. Only "H" positive, but "O" negative, often nonspecifically elevated by immunization or previous infections Antibody level maybe lower when have used antibiotics early. 22 Limitations of Widal test It is non specific and a positive test could be due to Infection by other salmonellae (as the antigen used for the test is also shared by other salmonellae) Recent vaccination for typhoid Past typhoid (already treated) 23 ENTERIC (TYPHOID) FEVER: TREATMENT Prompt administration of appropriate antibiotic therapy prevents severe complications of enteric fever and results in a case-fatality rate of <1%. The initial choice of antibiotics depends on the susceptibility of the S. Typhi and S. Paratyphi strains in the area of residence or travel. 24 RX… 25 1st line • Ciprofoxacin: 500mg PO BID for 5-7days • Ceftriaxone 1-2 gm IM or IV for 10 -14 days Alternative • Azithromycine 1 gm PO daily for 5 days • Chloramphenicol 500 mg Po QID for 14 days • Norfloxacin 400mg twice daily for 10 days 26 2.4 RICKETTISIAL DISEASES: (EPIDEMIC AND ENDEMIC TYPHUS) Rickettisiae are small intracellular bacteria that are spread to man by arthropod vectors, namely human body lice, fleas, ticks & larval mites. Epidemic Typhus (Louse born ): is caused by R.prowazekii and transmitted by human body louse (Pediculus humanus corporis). Endemic Typhus (Flea born) / Murine Typhus: is cause by R. thyphi which is transmitted by fleas. 27 PATHOPHYSIOLOGY In man rickettsia multiply in the endothelial cells of capillaries causing lesions in the skin, brain, lung, heart, kidneys and skeletal muscles. causes thromboses and small hemorrhages. However, tissue and organ injury is commonly due to increased vascular permeability with resulting edema, hypovolemia and organ ischemia. 28 Clinical Features (epidemic typhus) Incubation period of 1 week Abrupt onset of illness with prostration, severe headache and rapidly rising fever of 38.8 to 40.0 °C Cough s seen in 70 % of patients , myalgia may also occur which may be severe Rash, begins on upper trunk around 5thday and then becomes generalized, involving the entire body except face, palms and soles; at first, rash is macular, becoming maculopapular, petechial and confluent without treatment, although in black people, rash may be absent (spotless epidemic typhus) • Photophobia, with conjunctival infection and eye pain 29 signs of central nervous system involvement,may occur at the end of 1st week (meningo-encephalitis) 30 Endemic typhus (Flea borne typhus) Endemic typhus (also known as muri ne typhus) is a relatively milder. The incubation period is 1 - 3 weeks and followed by sudden onset of fever, rigors, frontal headache, pain in the back and limbs, constipation and cough (due to bronchitis). The fever becomes constant after the third day and associated with conjunctivitis and orbital pain. 31 Laboratory investigations; Serologic tests: • Indirect fluorescent antibody test • Weil-Felix agglutination test: nonspecific or sensitive Isolation of the organism by inoculation into laboratory animals 32 Complications of Endemic and Epidemic Typhus Skin necrosis, gangrene of digits, Venous thrombosis Interstitial pneumonia in severe cases Myocarditis Oliguric renal failure Parotitis 33 Treatment of rickettsial diseases Endemic Typhus Doxycycline 100mg bid PO for 7-15 days Chloramphenicol 500mg QID PO for7-15 days Epidemic typhus Doxycycline 200mg as single dose PO until the patient is afebrile for 24 hours. Delousing louse borne typhus 34 RELAPSING FEVER Definition Relapsing fever is an acute febrile illness caused by Borrelia species, presenting with recurrence of characteristic febrile periods last ing for days alternating with afebrile periods. Relapsing fever describes two distinct diseases: • Louse borne (Endemic) relapsing fever (LBRF):- transmitted by body louse( Pediculus humanis var corporis) • Tick borne (Epidemic) relapsing fever (TBRF)- transmitted by tick (Ornithodoros) 35 Etiology Relapsing fever is caused by Borrelia species, which are spirochetal gram negative helical bacteria. B. recurrentis is the only species that cause LBRF B. duttoni is the commonest causes of TBRF in sub-Saharan Africa 36 PATHOGENESIS Portal of entry, infected lice crushed into abraded skin. Incubation period, 5-10 days. High level spirochetemia. Patients’ producing neutralizing antibodies, clearing of the circulating strain Borrelia in 3-5 days New ANTIGENIC VARIANTS appear . Recurrence of clinical symptom/signs; up to 3-5 relapses may occur CLINICAL FEATURES Incubation period, 5-10 days, average 7 days. The range of clinical symptoms/ signs is wide. In a typical sever case there is abrupt onset of fever, 39-40. The following is the dominant clinical features: CLINICAL FEATURES, contd. SYMPTOMS Fever Headaches Arthralgia/myalgia Dry cough Epistaxis/gum bleeding SIGNS Temperature Tachycardia Hepatomegaly Splenomegaly Petichea/ Subconjunctival bleeding Jaundice Confusion/Meningism COMPLICATIONS Congestive heart failure Jaundice Bleeding diathesis Jarish- Herxheimer reaction: The first dose of appropriate antibiotic causes transient worsening of clinical symptoms/signs. Frequency, 35-100%, associated with increased mortality. COMPLICATIONS CONTD. Physiologic change: chills phase, rise in BP, pulse,and respiratory rate; BP falls dramatically . flush phase, There is a marked, but transient rise in circulating level of : TNF, IL-6, and IL-8 at the peak of the reaction (figure 2). DIAGNOSIS Demonstration of the organism; blood film. MANAGEMENT Aims of Management: Clinical cure Prevention of relapse Prevention/treatment of complications Antibiotic treatment: A number of antibiotics are effective: Anticipate the occurrence of Jarish- Herxheimer like reaction. In our setting we simply start with procaine penicillin 400,000IU IM and then observe for 12 hours and repeat blood film. Management of complications Supportive treatment: IV fluids for hypotension, diuretics for pulmonary edema. Prevention and control Better housing, reliable water supply, good personal hygiene. Insecticides like DDT for killing lice. MALARIA Defn: Is a protozoan disease transmitted by the bite of A. mosquito Transmission in 107 countries Affect 3 billion ppls worldwide ETOLOGY Four sp. Of the genus Plasmodium Others – rare P. falciparum – malignant malaria P. vivax – tertian malaria P. ovale – Tertian malaria P. malariae – quartian malaria NB: almost all deaths are caused by falciparum malaria EPIDEMOLOGY Is complex and varies considerably even within relatively small geographic area Endemicity: defined interms of parasitemia rates or palpable spleen rates in childern 2 – 9 yrs of age as Hypoendemic - < 10% Mesoendemic – 11 – 50% Hyperendemic – 51 – 75% Holoendemic - > 75% EPIDEMOLOGY Stable transmission – is constant frequent round infection Unstable transmission transmission is low, erratic or focal Full protective immunity is not acquired Symptomatic disease occurs at all ages Usually occurs in hypoendemic area EPIDEMOLOGY Principal determinants are The density of the vector Human biting habit Life span of A. mosquito Common in both low and highland and epidemics is commonly observed in highlands Temprature – < 16 -18 oc sporogony is inhibted stable unstable Mosquito life long short Mosquito bites frequent rare Human immunity high low epidemics No yes Eradication/ control difficult possible TRANSMISSION Is by the bite of mosquito(female anophilous) Transfusion malaria – blood transfusion, needle stick injury, sharing of needles, organ transplantation Incubation Period – short (no preerritrocitic cycle) c\f & mgt-same LIFE CYCLE AND PATHOGENSIS Divided into asexual and sexual sexual - Occurs inside a mosquito(definitive host) Asexual – it occurs in human body Two phases exoerytrocytic erytrocytic Life cycle (fig) HOST RESPONSE Activated nonspecific defense mechanism Because of rapture of erythrocytes and release of parasites – activation of the microphage and release of proitory mononuclear cell derived cytokines causes fever (> 40 oc) Sickle cell disease – decreased risk of infection (six fold) Impaired parasite growth at low oxygen tension CLINICAL FEATURE IP 18 days to six weeks (P. malarae) 10 to 14 day (others) Types and clinical features (tab) None specific symptoms followed by fever N\V. Febrile paroxysms – have three stages Cold stage : patient feels cold and has shivering Lasts for 30’ to 1 hr Characterized by vasoconstriction of vessels and rapid rise temperature CLINICAL … Hot stage Patient feels hot and uncomofortable Become delirious, lasts 2 – 6 hrs Sweating stage Physical Examination Will have profuse sweating and become very much exhausted Uncomplicated – fever, mild anemia, palpable spleen and liver Severe and complicated malaria (tab) CHRONIC COMPLICATIONS Tropical spleenomegally syndrome(HSM) It’s a syndrome resulting from unabnormal immunologic response to repeated infection Seen in some residents of endemic area (Tropical Africa and Asia) Characterized by Huge spleen (> 10 cm BLCM) with/out HM Hyperspleenism (anemia and pancytopenia) Marked elevation of serum IGM and antimalarial antibody Hepatic sinusoidal lymphocytes Peripheral B cell lyphocytosis TSM C/F: abdominal mass or drugging sensation in LUQ • Abdominal pain Patients are vulnerable to respiratory and skin infection and die of overwelming sepsis due to pancytopenia Parasite may not be detected and May evolve to malignant disorder Rx: Prophlaxes for long time usually for duration of exposure Commonly used are Chloroquin and myphyloquin Enlarged spleen and liver will regresss with effective prophylaxes Spleneoctomy is only indicated for these with failure of anti-malarial prophylaxes atleast given for six month DIAGNOSIS Peripheral blood film – Giemsa/wright’s stain Thin and thick film Thin film Rapidly air dried Fixed in anhyderous methanol and stained Intact RBCs could be seen Advantage spicious identification is simple Percent of RBC parasitized can be estimated DIAGN…. Thick blood film Dried throughly and stained without fixing Many RBCs lyzed during procedure Advantage Concentrates RBC (40 to 100 fold) Helps to determine parasite concentration NB – single BF doesn’t rule out malaria Preferably done during febrile episoides If and if blood smear negative in endemic araes repeat at least every 12 to 24 hrs for 2 days DIAGN… Other IX: cbc Blood glucose LP and CSF analysis (on indication) BUN/CR, LFT TX A. Benign forms of malaria (Plasmodium malariae, vivax, ovale): Chloroquine is effective Dose: Initial dose is 600 mg PO followed by 300mg after 6, 24 and 48 h subsequently. Treatment of uncomplicated falciparum malaria A)Artemether-Lumefantrine: (Coartem 20/120): Adult Dosage: < 35 kg: 3 tabs PO BID for 3 days > 35 Kg: 4 tabs PO BID for 3 days B)Quinine: Adult dose : 600 mg PO TID for 5 – 7 days alone or in combination with + Tetracycline 500 mg PO QID or Doxycycline 100mg PO /day for the same period other option mefloquine& fansidar TREATMENT OF SEVERE AND COMPLICATED FALCIPARUM MALARIA: Admit patient i) Quinine : is drug of choice for severe and complicated malaria. Dosage and Adminstration: Where IV administration of quinine is possible Loading dose: Quinine 20 mg salt/kg of body weight by infusion over 4 hours, in 5 % dextrose in saline (5-10 ml/kg of body weight depending on the patient's overall fluid balance). Maintenance does: Twelve hours after the start of the loading dose, give quinine 10 mg salt/kg of body weight in dextrose saline over 4 hours. Repeat the same dose of quinine until pt is able to take orally. 64 ii) Artesunate injection (if available) : 2.4 mg/kg IV or IM stat followed by 1.2 mg/kg at 12 and 24 hrs and then daily. B) Supportive treatment: • Bring down fever (cold sponges, paracetamol) • Administer glucose IV or PO to prevent hypoglycaemia and encourage early PO intake of food • Ensure adequate fluid intake, check input and output and control water and electrolyte balance (beware of pulmonary edema due to fluid overload). • Consider transfusion in severe falciparum malaria with high parasitemia (> 20% of erythrocytes affected by plasmodium) • Check renal function tests and blood sugar (beware of hypoglycemia). • For comatose or unconscious patients proper nursing care mandatory 65