Academy of Sciences of Republic of Uzbekistan
Institute of Genetics and Plant Experimental Biology
Using diazotrophic rhizobacteria and bacterial
antagonists in salt stress environments for enhancing
adaptation, resistance of basic agricultural cultures
(cotton, wheat) on salinity, drought and diseases and
development ecologically pure production
Head of the Project :
Dr. Rasulov B.
O b j e c t ive s :
• Study of complex impact of diazotrophic rhizobacteria and bacterial
antagonists on adaptation to salt stress and resistance to pathogens of
cotton and wheat cultivars in salt affected environments and reducing of
the mineral fertilizers usage in agriculture. Development of new
agrobiotechnology to obtain ecologically pure agricultural products via
application of ecologically friendly microbe biotechnologies, reducing
energy and resources in agriculture, and recovering the cycle of basic
biogenic elements in the artificial agrocenosis.
Ta s k s :
• 1). Isolation of diazotrophic rhizobacteria and bacterial antagonists from
the rhizosphere of cotton and wheat, identification of bacterial isolates;
screening of active nitrogen fixing and antagonist strains against
phytopatogens (Fusarium oxysporum f.sp.vasinfectum and Verticillium
dahleae).
• 2). Study of secondary metabolites (auxins, gibberellins,
exopolysaccharides) of diazotrophic rhizobacteria and their ability to
mobilize insoluble phosphates.
• 3). Study of expression some genes, responsible for salt tolerance in
bacteria in salt-affected and water deficient environments
• 4). Development of bacterization of agricultural cultivars (via root and
leaf) for creating artificial beneficial microbe populations in the cotton and
wheat rhizosphere.
Expected results from the project:
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The mechanisms of complex impact of nitrogen fixing bacteria and bacterial
antagonists on adaptation of cotton and wheat to salt stress will be revealed.
New agrobiotechnologies of bacterial treatment of cotton and wheat will be
developed.
Most active nitrogen fixing bacteria and bacterial antagonists will be selected
and molecular-genetic characterization will be clarified.
The functional activity of bacterial strains in salt stress will be researched.
The role of secondary metabolites in growth and development of cotton and
wheat in salt stress will be studied.
Expression of some genes, responsible for salt resistance in bacteria will be
determined.
Optimal conditions for formation of beneficial microbe populations in the cotton
and wheat rhizospheres will be elucidated.
Recommendations and agrobiotechnologies of complex application of beneficial
bacterial strains (nitrogen fixing and antagonist bacteria) for enhancing
adaptation, resistance of cotton and wheat to salt stress, drought and
pathogens will be developed.
Proposed activities within the framework of the
project:
• 1. Isolation of diazotrophic rhizobacteria and bacterial antagonists from
the rhizosphere of cotton and wheat, identification of bacterial isolates;
screening of active nitrogen fixing and antagonist strains against
phytopatogens (Fusarium oxysporum f.sp.vasinfectum and Verticillium
dahleae).
• 2). Study of expression some genes, responsible for salt tolerance in
bacteria in salt-affected and water deficient environments
• 3). Development of bacterization of agricultural cultivars (via root and
leaf) for creating artificial beneficial microbe populations in the cotton
and wheat rhizosphere.
• 4.
Trainings within the project
T H A N K S F O R YO U R AT T E N T I O N !