Presentation of ALGEBAKT project. Antibacterial effect of arctic macroalgae species DMV, PhD, Margarita Novoa-Garrido Alta, Norway, Sept, 6th - 8th, 2010: The 7th Circumpolar Agricultural Conference "Circumpolar Agricultural and Land Use Resources - Prospects and Perspectives for Circumpolar Productions and Industries" The MABIT-programme is an industrial R&D program for Northern Norway. Bioforsk Nord Bodø. Bioproduction and ecosystems along the coast Coastal ecology - possibilities for commercial exploitation of coastal and marine resources without damaging ecosystems. Our main focus is the algae. Background for the project • Antibiotics: therapy and growth promotion • Ab-resistance • Antibiotics banned in Europe as antibiotic growth promoters since 2006 It was on a short-cut through the hospital kitchens that Albert was first approched by a member of the Antibiotic Resistance. Antibiotic resistance test of P. aeruginosa strain Foto: Unni Lorentsen A. nodosum Pelvetia canaliculata Palmaria palmata Alaria esculenta ALGINATE Vegetables in seaweed project in Bioforsk Nord. Foto: I. Øvsthus A. nodosum L. hyperborea SEAWEED MEAL Sheep in seaweed meal project in Bioforsk Nord. Foto: H.J. Skulstad Algae composition • • • • • • • Dietary fibre (<8% DM) Protein (5-10 % DM) Fat (2-4 % DM) Carbohydrates (45-60 % DM) Vitamines: A, C, E, B1, B2, B3, B8, B9 Minerals Secundary bioactive metabolites Macroalgae biologiske effekter • • • • • • • Antibakterial Antivirus Antitumor Antioxidant Antiinflammatory Immune stimulans Growth stimulant Stomach up to 102 Lactobacilli, streptococci, yeast Animal health Jejunum and ileum 104-106 CFU/ml Lactobacilli, coliforms, Streptococci, fusobacteria, clostridia, bacteriodes, bifido bacteria Environment Immune system Bacterial population Diet Colon 1010-1012 CFU/ml Lactobacilli, bifido bacteria, eubacteria, fusobacteria, coliforms, clostridia, veillonella, yeasts Photo: Birger Volden Some research results • Animal husbandry: nr. of E. coli intestine, shedding of E. coli O157 and Salmonella, nr. adherent Lactobacillus sp. Innate immunity and B lymhocyte activity. • Aquaculture: Antibacterial effect on fish pathogens such as Vibrio sp. and Aeromonas sp. • Furanones, organohalogens, floratannins, fucoidans, haloperoxidases, alginate Aims of the project • Generate knowledge about bioactive components with antibacterial effect in macroalgae species adapted to the northern Norwegian climate to get a better overview of the potential for developing new products based on their antibacterial property. Material and methods • Bacteria sheep: Lactobacillus sp., Enterococcus faecium, Enterococcus faecalis, different E. coli strains • Bacteria fish:Vibrio sp., Pseudoalteromonas sp., Aeromonas sp. • Macroalgae: Four red species and two brown species • Minimum inhibitory concentration (MIN) • Liquid chromatography-mass spectrometry Synergy with other ongoing prosjects • «Natural sources of antioxidants – a necessity for animal health and welfare and product quality in organic livestock production.» • The research Council of Norway. • Effekt of feeding macroalgae on the milk quality of dairy cattle. • Effekt of feeding macroalgae on intestinal bacterial flora and immune system of sheep. Future visions • Screening • Identification of biologically activ components • Animal experiments THANK YOU FOR YOUR ATTENTION!