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• Self renewal capacity.
• Long term culture mode.
• Elevated proliferation rate.
• Insignificant response to naïve cord blood T cells.
• Extremely high probability of cure.
• Master cells.
• Repair, Regenerate & Replace damaged cells.
• Form heart, liver, nervous system, spinal cord etc.
• Over 200 types of tissues – Clinical significance.
• Ability to divide, differentiate continuously – to address diseases like Heart failures, Alzheimer’s,
Parkinson’s, Thalassemia, Leukemia, Liver,
Diabetes problems.

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Blastocyst Birth
Adult
Fetal source
Stem Cell
ESC-P FTSC
P,M
CBSC
UCSC
ASC
PSC
ASC
BMSC
P, M
P, M
FTSC -Fetal Tissue Stem Cells
CBSC -Cord Blood Stem Cells
UCSC -Umbilical Cord Stem Cell
ASC -Amniotic Stem Cell
PSC -Placental Stem Cell
ASC -Adult Stem Cell
BMSC -Bone Marrow Stem Cell

• Umbilical Cord/Blood Stem
Cells
• Traditional discard byproduct of the birth process
• Multipotent - stem-cell - rich blood found in the umbilical cord has proven useful in treating the diseases
• Umbilical cord blood stem cell transplants - less prone to rejection than either bone marrow or peripheral blood stem cells - the cells have not yet developed the features that can be recognized and attacked by the recipient's immune system
• Also, because umbilical cord blood lacks welldeveloped immune cells, there is less chance that the transplanted cells will attack the recipient's body, a problem called graft versus host disease
• Versatility and availability of umbilical cord blood stem cells - potent resource for transplant therapies
• These cells have no morbidity, it is possible to isolate huge numbers and they do not belong to an ethically controversial source
Fewer limitations - not tumorigenic, maintain for several serial passages - short population doubling time

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• Collection process is easy
• There is no harm done either to the mother or the baby
• High probability of HLA matching
• Medical waste – Rich source of high therapeutic potential stem cells

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• Peripheral Blood Stem Cells
• While most blood stem cells reside in the bone marrow, a small number are present in the bloodstream. These multipotent PBSCs, can be used just like bone marrow stem cells to treat leukemia, other cancers and various blood disorders
• PBSCs are easier to collect than bone marrow stem cells
• PBSCs - less invasive treatment option than bone marrow stem cells
• PBSCs are sparse in the bloodstream
Collecting enough to perform a transplant can pose challenge

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Present throughout the body from head to toe
• Brain
• Liver
• Skin
• Dental pulp
• Adipose tissue
• Placenta
• Menstrual blood
• Synovial fluid
• Umbilical cord
• Heart
• Pancreas
• Intestine

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Bone Marrow
Mesenchymal
S tem Cells
Hematopoietic
S tem Cells
M S C are the most preferred cell types in clinical applications
During the past 50 years, intensive studies into the characteristics of hematopoietic stem cell transplantation immunology with the emergence of new immunosuppressant and anti-infective drugs have significantly improved the clinical result of hematopoietic stem cell transplantation .

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• Already somewhat
Inducement may be simpler specialized:
• Immune hardy: Recipients who receive the products of their own stem cells will not experience immune rejection
• Flexible: Adult stem cells may be used to form other tissue types
• Mixed degree of availability: Some adult stem cells are easy to harvest and others, such as neural (brain) stem cells, can be dangerous to the donor
The surprising property of adult stem cells: transdifferentiation

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• Acute Leukemia
• Chronic Leukemia
• Myelodysplastic Syndrome
• Myeloproliferative Disorder
• Lymphoproliferative Disorder
• Familial Erythrophagocytic
• Lymphohistiocytosis
• Histiocytic Disorder
• Inherited Metabolic Disorder
• Platelet Abnormality
• Plasma Cell Disorder
• Thalassemia
• Other Malignancies

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• Infusion of healthy cells to replace diseased or damaged ones
• The healthy replacement stem cells will integrate into the body and give rise to more cells that can all take on the necessary functions for a specific tissue

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Today
• Leukemia's
• Lymphomas
• Multiple myeloma
• Radiation sickness
• Autoimmune Dx
• Tissue repair and Burns
• Breast and Ovarian cancers
Near Future
• Diabetes
• Cardiovascular Dx
• Spinal Cord Injuries
• Skin Rejuvenation
• Rheumatologic
• Orthopedics
• Stroke
Sources include: www.marrow.org; www.viacord.com; www.cryo-cell.com

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For
Solid Tumors
Renal Carcinoma
Melanoma
Ovarian Cancers
Metastatic Breast Cancers
In utero HSC transplantation
In vitro Generation of Gamestes
Treating
Abdominal Wall Defects
Umbilical Hernia
Spina Bifida

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• Health conscious individuals
• Individuals with family history of cancer, diabetes and heart diseases
• Individuals diagnosed with cancer
• Individuals - exposed to toxins or harmful radiation because of their jobs
• “First responders”, who may be exposed to lethal levels of radiation

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• Tran-Scell is state of the art stem cell banking and processing centre.
• Tran-Scell is highly equipped with expertise and optimal microenvironment for processing and preserving your child’s precious stem cells for all the potential future medical requirements.
• Personal commitment from enrollment, collecting the source, processing the stem cells, cryopreserving the customized stem cell units for future therapeutic options, reporting associated with safe service delivery.

• Stringent quality control and quality assurance ensured.
• Standard operating procedures and automated processing protocols followed.
• Samples are stored in an fire and earthquake proof vaults with 24x7 monitored ambience.
• Emergency UPS, Access restriction systems and
Backup arrangements installed.

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• Umbilical Cord Blood Collection, Processing &
Storage of Haemopoitic stem cells.
• Umbilical Cord Tissue Collection, Processing, Cell expansion & Storage of harvested Mesenchymal stem cells.
• Processing of Bone Marrow for adult stem cells & storage.
• Processing & storage of Peripheral Blood stem cells.
• Generation of stem cells from various sources for therapeutic applications on various dread full diseases.
• Private Banking facilities.

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• Isolation & Expansion of Stem cells from Dental
Pulp for therapeutic application on dental disorders.
• Isolation & Expansion of Acinar cells from Human lacrimal gland for treating Dry eye syndrome.
• Isolation of stem cells from Human adipose tissu e.

S afety and S ecurity
• TranS cell maintains optimal conditions for processing and storing stem cells
• Quality control measures for each stage of the stem cell banking process

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• Automated Processing using SEPAX.
• Manual processing allows trained scientists to perform quality control at every step
• Standard Operating Procedures – Batch wise specifications
• Certificate of Analysis of the final finished personalized product
• On average, the process employed recover over 90% of total nucleated cells and high density of mesenchymal stem cells from the sample

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• Initial quarantine
• Long-term storage in liquid nitrogen maintains temperatures at -196°C, far below the recommended minimum standard for storage at -150°C
• On-site tank feeds a constant supply of liquid nitrogen, even during a power outage
• Electronic systems constantly monitor temperature and liquid nitrogen levels
• Functional In-built remote alarm system

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• HLA for every sample
• Associate Banking
• Formulation
• For Immediate Therapy & Future Applications
• Application Specific
• Duplicates – Multiple Vials
• Expansion – Whole Diseases Management
• Automated and Semi automated process
• Regenerative medicine factor

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