Chapter 8
Leukocyte Differentiantion Antigens and
Cell Adhesion Molecules
Contents
 PartⅠ Membrane molecules of immune cell
 PartⅡ Definition of leukocyte differentiation
antigen and CD
 PartⅢ Definition, classification and functions
of adhesion molecules
 PartⅣ Clinical application of CD and
adhesion molecules
PartⅠ Membrane molecules of immune cell
Receptors: TCR, BCR, CR, CKR, FcR
ClassⅠand classⅡ MHC molecules
CD molecules: CD1~339
Cell Adhesion Molecules
PartⅡ Definition of leukocyte
differentiation antigen and CD
 Leukocyte differentiation antigen: Cell surface
molecules expressed (or disappeared) during
different developmental and differential phases,
activation or inactivation process of blood cells.
PartⅡ Definition of leukocyte
differentiation antigen and CD
 Leukocyte differentiation antigen
 CD: cluster of differentiation.
The same differentiation antigen recognized by
different monoclonal antibody from different lab
are called CD.
CDs which take part in T cell recognition,
adhesion and activation
CDs which take part in B cell recognition,
adhesion and activation
PartⅢ Definition, classification and
functions of adhesion molecules
Ⅰ. Definition
Cell adhesion molecules (CAMs) are cell
surface proteins involved in the interaction
of cell-cell or cell-extracellular matrix.
CAMs take effect by the binding of
receptor and ligand.
Ⅱ. Classification
Integrin family
Selectin family
Immunoglobulin (Ig) superfamily
Cadherin family
Mucin-like family
Other adhesion molecules
1. Integrin family
Integrins consist of α and β chains.
According to β subunits, Integrins are divided
into eight groups: β1- β8
VLA-4(Very Late Antigen-4)------VCAM-1
LFA-1(Lymphocyte Function-associated
Antigen-1)------ICAM-1,2,3
 MAdCAM-1 (Mucosal Addressin Cell Adhesion Molecule-1)
 TSP-1 ((Thrombospondin一1):凝血酶敏感蛋白一1
Integrin and platelet aggregation
(Karp, 2001)
Integrins promote immune response
2. Selectin family
Selectins consist of one peptide chain.
The three family members include: Eselectin, L-selectin, and P-selectin.
3. Ig superfamily(IgSF)
The structure of these adhesion
molecules resemble that of Ig.
CD4, CD8, CD2(LFA-2), CD58(LFA-3),
VCAM-1, ICAM-1,2,3
4. Cadherin family
 E-cadherin------ Epithelia cell
 N-cadherin------ Nerve cell
 P-cadherin-------Placenta
5. Mucin-like family
CD34, GlyCAM-1(glycosylation dependent
cell adhesion molecule-1)
PSGL-1(P-selectin glycoprotein ligand-1)
6. Other adhesion molecules
CD44
Ⅲ. Functions
1. Participate in development and
differentiation of immune cells
 CD2----LFA-3
 LFA-1----ICAM-1
-------Participate in development and maturation of
thymocytes.
2.Participate in immune response and regulation
3. Participate in the adhesion of leukocyte
and vascular endothelial cell during
inflammation.
4. Participate in lymphocyte homing
Lymphoid stem cell migrate to central
lymphoid organs.
Mature lymphocyte migrate to peripheral
lymphoid organs
Recirculation of lymphocytes
Lymphocyte migrate to the sites of
inflammation
5. Participate in regulation of apoptosis
• Integrins inhibit cell apoptosis
What should you know by the
end of this lecture?
Definition of leukocyte differentiation
Antigen,CD and adhesion molecules
Classification and function of adhesion
molecules