Expert of yeast origin nutrients for
“Microorganisms”
Johnny Yin
18 April, 2014
Contents
1
Introduction about Angel yeast Co.,Ltd.
2
Angel yeast extracts – Good nutrient-sources for microoganisms
3
Application of Angel yeast extract
1. Introduction of Angel Yeast Co.,Ltd.
1.1 About us >
1.2 Global net work >
1.3 Our history >
1.4 Main business >
1.5 Angel’s value chain >
1.6 Employees >
1.7 Advantages >
1.1 About us
• Angel yeast, founded in 1986, is a listed high-tech yeast company in
China.
• It is one of the largest global yeast and yeast extract manufacturers
with 12 factories in China and Egypt up to now.
1.2 Global network
12
Factories
4
12
Technical centers
140
Yeast extract plants
Countries
1.3 History
Project started
1986
Public company
1989
First facilities
2000
No.1 in Asia
2003
First yeast
extract facilities
2008
Today
Global supplier
The fastest growing company in global yeast industry
•
Angel yeast is estimated to grow at a CAGR of 20% 2011-2012, while the global
yeast market’s CAGR is to be 8.6% (Foodnavigator.com, 2012-04-05).
Sales, RMB
35
30
25
20
15
10
5
0
12
20
10
20
08
20
06
20
04
20
02
20
00
20
CAGR 2011-2012
1.4 Main business
Main business
•
•
•
•
•
•
•
Bakery
Yeast extract-savory
Yeast extract-fermentation
Beverage & ethanol
Human health
Enzymes
Animal nutrition
1.5 Angel’s Value Chain
Lab
Yeast extract
Inactive yeast
Technical support
Experiment
Microorganism
Yeast extract
Industrial fermentation
Package
Trading
Product
Brewing
Food
Agriculture Environment Chemical
Bakery
Yeast extract
Enzyme
Bio-fertilizer
Seasoning
Inactive yeast
Yeast extract
Yeast extract
Feed
Energy
Pharm
Alcohol yeast
Wine yeast
Enzyme
Enzyme
Yeast extract
Yeast cell wall
Feed yeast
Enzyme
Active yeast
Yeast extract
Inactive yeast
Our global partners
1.6 Employees>
• 1331 employees, 70% of which are professional people
• Over 100 researchers work together with universities.
• 7 international experts employed as senior technical consultants.
Jan Hille ((Netherlands)
Yao Juan
Rosenberg (Netherlands)
Li Xiao
Kuang Jinbao
He Chongyue
Tilak (U.S.)
(Taiwan, China)
Li Pei
Li Zhijun
1.7 Advantages
1.7.1 Strong production output >
1.7.2 Professional production process>
1.7.3 R&D strength >
1.7.4 Quality, safety and consistency >
1.7.5 Certification>
1.7.6 Environment protection >
1.7.7 Global professional service >
1.7.1 Strong production output >
Total output of
Yeast is 120.000 tons, Yeast Extract is
Yichang Plant
Chongzuo Plant
46.000 tons.
Yili Plant
Binzhou Plant
1.7.2 Professional production process
1.7.3 R&D strength >
• 4 sub-headquarters with technical centers
• National yeast technology research and
promotion center
• Post-doctoral scientific research workstation
• R & D team led by PhDs and experts
• National recognized laboratory CNAS
• Basic research and Application research
laboratory
• Own the first-class test device and pilot plant.
1.7.4 Quality, safety and consistency >
• EU standard design production
line
• Stable and consistent quality
applies to all production plants
• Distributed control system
• Siemens PCS7 process control
system
• Whole-course CIP cleaning
• SAP-ERP Management flat
1.7.5 Certifications >
BRC
CNAS
SEDEX
ISO9001
HALAL
ISO14001
KOSHER(OU)
ISO22000
1.7.6 Environment protection >
• First class environment protection facilities in each plant
• The high-concentration organic waste water was made into bio-organic
fertilizer.
1.7.7 Global professional service >
• We do not only provide the best
quality products, we also provide
technical solutions for all our serving
industries
• Set up global baking centers
• Over 200 application engineers
2. Angel Yeast Extracts
– Good nutrient-sources for microoganisms
2.1 What’s yeast extract >
2.2 Why choose yeast extract for fermentation industry >
2.3 Range of Angel’s typical products >
2.1 What’s yeast extract?
Yeast extract is a kind of :
• 100% animal free natural ingredient that is
made from yeast.
• High performance and stable sources of
nutrients for microorganisms
• Clean and safe raw materials for industrial
fermentation and culture media
Process
of yeast extract production
2.1.1 Molecular weight distribution
2.1.2 Profile of Amino acids
A0128
A0316
A0418
OX3098
OX6919
7
Concentration (%)
6
5
4
3
2
1
0
Asp Thr Ser Glu Gly Ala Val Met Ile Leu Tyr Phe Lys His Arg Pro
Amino acids
2.1.3 Profile of free Amino acids and Total amino acids
Free Amino acids / Total Amino acids ×100
2.1.4 Trace elements
Name of elements
Ca
Mg
Zn
Fe
Cr
Mn
K
Na
As
Se
Pb
Concentration（mg/kg）
246
149
86
33
1.26
0.08
9172
2022
0.03
0.002
0.19
2.1.5 Vitamins
Name of vitamins
Concentration（mg/kg）
Vitamin B1
2.7
Vitamin B2
56.9
Niacin
336
Vitamin B6
17.6
Pantothenic acid
29
Free D-Biotin
500 （μg/100g）
2.1.6 Nucleic acid and Nucleotides
Concentration (%)
3.0
2.5
2.0
1.5
1.0
0.5
0.0
A0128
A0316
A0418
OX3098
OX6919
0.12
Concentration (mg/kg)
A0128
A0316
A0418
OX3098
OX6919
0.10
0.08
0.06
0.04
0.02
0.00
RNA
UMP
CMP
GMP
IMP
UMP, CMP, GMP,IMP and AMP
AMP
2.1.7 Functionality of yeast extract
• Increase biomass
• Improve the accumulation of metabolites
2.1.8 Stability of yeast extract
Two key indicators: Total nitrogen and Amino nitrogen,
For example: Stability of Yeast extract FM902
2.2 Why we choose yeast extract for fermentation industry?
• Clean and Safe
- Clean and safe natural material for culture media and industrial fermentation
- Animal free, no risks of illness
- GMO free, Non allergen
- Kosher and Halal certified.
• Stable quality
- Produced with strict quality control system.
- Large industry scale production
- No quality change in different seasons
• High performance
- Advanced production technology
Beside yeast extract, Angel also produce yeast peptone and inactive yeast for
fermentation industry
2.3 Range of Angel’s typical products
Microfiltered yeast extract
FM888 Powder
Lab, Biopharmaceuticals
FM803 Powder
Nutrients-enriched yeast extract
Probiotics, Starter culture
FM505 Powder
FP101
Powder
Yeast peptone
Probiotics, culture media
LP101
Paste
FM808 Powder
FM902 Powder
Standard yeast extract
Industrial fermentation
LM800 Paste
LD00
Liquid
FM801 Powder
Autolyzed & Inactive yeast
Antibiotics, APIs
YP101
Powder
FM701 Powder
Economical yeast extract
Agriculture and environment
LM815 Paste
Which are the most popular
products in lab research?
Products
FM888
FP101
Angel Microfiltered yeast extract FM888
• Features and Advantages
- High solubility
- Easily assimilated by microorganisms and cells
- Rich in peptides and growth factors
- Low impurities
• Application
- Lab research
Dehydrated culture media, Cell culture, Diagnostic media
- High value-added industrial fermentation
Insulins, Hormones, Hyaluronic acids
Angel yeast peptone FP101
• Features and Advantages
- Good replacement of Animal origin peptone
- Could be combined with yeast extract
- Rich in peptides
• Application
- Lab research
Microorganisms culture, small scale fermentation
- Industrial fermentation
Probiotics, Enzymes, Hormones, Organic acids
Which are the most popular
products in industrial fermentation?
Products
LD00
LM800
FM808
FM902
Angel standard yeast extract FM808
• Features and Advantages
- Good solubility
- High nitrogen level
- Rich in peptides
- Light color
• Application
- Lab research
Dehydrated culture media
- Industrial fermentation
Insulins, Hormones, Hyaluronic acids, Probiotics
Angel standard yeast extract FM902
• Features and Advantages
- Total soluble in water
- High nitrogen level
- Cost effective
- High consistency
• Application
- Industrial fermentation
For >60 types of fermentation-based products
Angel standard yeast extract FM902
Angel standard yeast extract FM902
Angel standard yeast extract FM902
Angel standard yeast extract FM902
Industrial fermentation
FM902
Agriculture
Amino acid
Enzyme
Hormone
Organic acid
Probiotic
Nucleotides / Nucleosides
Poly saccharide
Biological preservatives
Antibiotic
Mashroom
Rhizobia
Biological bait
Effective Microorganisms
Bio-fertilizer
Waste water treatment
Environment
Environmental microorganisms
Angel standard yeast extract LM800 and
LD00
• Features and Advantages
- Paste and Liquid form of yeast extract FM902
- Rich in peptides
- Cost effective
- High consistency
• Application
- Industrial fermentation
For >60 types of fermentation-based products
How to judge the quality?
Better yeast extract perform better
solubility
Better yeast extract perform lighter
color
Better yeast extract perform higher
amino nitrogen
Yeast extract should contain low level
active microbes
Less biomass and productivity means
poor quality of yeast extract
Baker’s yeast extract is better than
Brewer’s yeast extract
Baker’s yeast extract must get
Kosher/Halal certificates
Yeast extract contain rich protein
Depends
Depends
Depends
True
Depends
True
True
Good solubility means readily assimilated nutrients,
but it depends on specific needs.
Some fermentation need light color
Higher amino nitrogen product is suitable for seed
culture and probiotics, not all the fermentation
High level active microbes cause contamination
40% of this situation means unsuitable process,
condition or culture media formula
Brewer’s yeast extract is not stable which come from
spent brewer’s yeast
Clean and safe
Depends It depends on the customers specific needs
3. Application of Angel Yeast Extract
3.1 Summary of Angel yeast extract application >
3.2 Some experiments of Angel yeast extract >
3.1 Summary of Angel yeast extract application
 Biomass products industry
- Starter cultures and probiotics for human food products
- Cultivation of mashroom and macrofungi
Recommendation: FM808, FM803, FM505, FP101
 Culture media industry and lab reagent
- Bench scale research
- Dehydrated culture media, Diagnostics, Cell cuture
Recommendation: FM888, FM808
 Bioactive molecules and chemicals industry
- Amino acids and peptides
- Organic acids, Vitamins, Nucleotides, Enzymes, Alcohol
- Vitamins, Antibiotics, Hormones, Biological preservatives
- Other types of large scale fermentation
Recommendation: FM808, FM902, LM800, LD00, YP101
 Agricultural and environmental industry
- Sewage treatment, Bio-pesticide, Bio-plastic
- Probiotics for agriculture and environment
Recommendation: FM902, FM801, FM701, LM815, LP101
3.2 Some experiments of Angel yeast extract
3.2.1 Morphology of 4 types of typical microorganisms >
3.2.2 Some experiments of Angel yeast extract >
3.2.1 Morphology of 4 types of typical microorganisms
after using yeast extract and other type of organic nitrogen
Streptomyces lincolnensis
A
Lactobacillus bulgaricus
B
A
Bacillus subtilis
A
B
Acetobacterium
B
“A”—Another type of organic nitrogen
“B”—Angel yeast extract
A
B
3.2.2 Angel standard yeast extract FM808 for
Lactobacillus thermophilus growth
Strain: Lactobasillus thermophilus
18
Medium: Yeast Extract FM808
0.3%，Glucose 2%, etc.
16
14
12
10
pH: 6.5
8
Angel yeastF M888
extract
安琪酵母浸粉
FM808
6
国外某试剂级酵母浸粉
Control
4
2
0
12
Result: Yeast extract FM808
could improve the growth of
Lactobacillus thermophilus well
16
20
24
28
32
36
40
44
48
100
80
60
安琪酵母浸粉
Angel yeastF M888
extract
40
Control
国外某试剂级酵母浸粉
20
0
12
16
20
24
28
32
36
40
44
48
FM808
3.2.3 Angel standard yeast extract FM902 for
the growth of E.coli and Absidia coerulea
The growth curve of E.coli,
comparison of FM902 and Brewer’s
yeast extract
The conversion rate of HC(from RSA),
Absidia coerulea, yeast extract FM902
3.2.4 Angel nutrients enriched yeast extract FM803
for probiotics
Strain: Lactobacillus rhamnosus
Medium: MRS
Strain: Lactobacillus helveticus
Medium: MRS
2
1.2
1
1.6
0.8
FM
803 yeast extract FM803
Angel
0.8
OD600
OD600
1.2
0.6
Angel
FM
803 yeast extract FM803
0.4
Control
国外某试剂级酵母浸粉
0.4
Control
国外某试剂级酵母浸粉
0.2
0
0
0
3
6
9
12
15
时间(h)
18
21
24
27
30
0
3
6
9
12
15
时间(h)
18
21
24
27
30
Lactobacillus needs complicated nutritional condition for its growth and acid
production. Yeast extract is rich in amino acids, small peptides and growth
factors which Lactobacillus needs. Yeast extract FM803 is a kind of free amino
acids enriched product and could improve the growth of Lactobacillus.
3.2.5 Angel standard yeast extract FM902
For L-Tryptophan
Fermentation culture medium(%):
yeast extract FM902, glucose 2 %,
ammonium sulfate 0.4%, potassium
dihydrogen phosphate 0.15%, etc.
Fig.1 The effect on L-Tryptophan when
different ratio of yeast extracts were used
Conclusion:
FM902 is rich in small peptides and
free amino acids, and it also
contains appropriate polypeptides,
vitamins and trace elements. Thus
FM902 is suitable for the
fermentation of L-Tryptophan, 2% is
a proper level of yeast extract ratio.
Technical support
Department of Microbial Nutrition Research
• The research and development of new products
• The optimization of culture media formula
• The suggestion of fermentation process
• Development of tailor-made products
Test and scale up platform
HPLC
GC-MS
Pilot Plant
Atomic Absorption Spectrometer
Powder Instrument
Platform for fermentation control
GC
Drawing Instrument
Amino acid analyzer
Ultrasonic Processors
The membrane separation system
Spray drying system
Contact information
Any question, please feel free to contact me:
Johnny Yin
Angel Yeast Co.,Ltd.
Address: 168 Cheng Dong Avenue, Yichang, Hubei, China. 443003.
Email: yinqiang@angelyeast.com;
johnnyyinq@gmail.com
THANK YOU!