Lois Sowden-Plunkett
Sept, 2012
Office of Risk Management
http://www.uottawa.ca/services/ehss/biosafety.htm
1. Challenge your
understanding
2. Establish good laboratory
practices
3. Prevent contamination
(you and your work)
4. Ensure research funding
Because research is now
continues $$$
interdisciplinary, it is now necessary
to retool yourself with new skills
and new understanding.

BIOHAZARD

BIOSAFETY

BIOSECURITY

BIOSURETY
A potentially infectious agent or hazardous
biological material that presents a risk or
potential risk to the health of humans,
animals, plants, and the environment
CFIA, PHAC, EC, DFAIT, TC, TRICOUNCIL &
OTHER FUNDING SOURCES, NIH....
viruses,
bacteria,
fungi,
parasites,
biological toxins,
prions, and
other micro-organisms
or genetic systems,
by virtue of
their replicative
properties, are
potentially
harmful to
humans,
animals, plants
and/or the
environment.
Recombinant DNA,
cultured cell lines,
tissues and
anatomical
specimens from
human or animal
subjects, and blood
and other bodily
fluids are also
potentially
infectious unless
tested and proven
otherwise.
• found in your intestines
• aid in food digestion
Extensively used for
recombinant DNA
application
• E.coli synthesize vitamins
B1,B2 & K.
• do not cause any harm if are
in limited number.
• deficiencies of these
vitamins cause many diseases
DH5 alpha
BL 21
XL1Blue
• beware of your antibiotics
which can destroy your E.coli
June 2, 2011
World Health
Organization
Sept 18, 2012
mutation of the E. coli
bacteria in Europe
E-0157:H4
E-0157:H7
(first reported in 1982)
1,500 sick,
470 have developed
a rare kidney failure
complication,
18 dead
unlabelled and/or
unbranded ground
beef products
available for sale from
August 24 through
September 16, 2012

P. aeruginosa

P. stutzeri

P. fluorescens




Ubiquitous in the
environment
Opportunistic
affects humans,
animals and plants
Survival out of host
(months on dry surfaces)
Risk groups
Characteristics




Hepatitis B,C & HIV
Don’t assume because you
know the person there is no
risk
UPEI June 27, 2012
300 Students at potential risk

Don’t even assume you don’t
have it.
The testing involved a lancet
and a receptacle for the blood.

If it’s not tested, it’s not safe!
The lancets were single use
only, but the receptacles were
used by several students.
UNIVERSAL PRECAUTION
The combination of measures employed when handling
biohazardous materials to:
 Protect personnel from exposure to infectious
agents
 Prevent environmental release and contamination
It’s good for you,
it’s good for the science and
it’s the law!
Safety Principles:
1. Administrative controls
2. Engineering Controls
3. Practices and Procedures
4. Personal Protective Equipment
In practical terms, this means:
Training, risk assessments, authorizations,
certification of rooms and equipment,
appropriated practices, health assessment
Measures employed to protect biohazardous
materials, or critical relevant information,
against theft or diversion by those who
intend to pursue intentional misuse.
(selected agents, U99, BSE)




Physical barriers
 Buildings, doors, locks, key card access
Psychological barriers
 Security personnel, cameras
Monitoring Activities
 Patrols, monitoring by support staff
Personnel Clearance
 Access to authorized personnel only
What is BIOSURETY?
Biosurety is defined as the
combination of security, biosafety,
agent accountability, and personnel
reliability needed to prevent
unauthorized access.
To be safe and compliant
it is really quite easy, it’s
all about:
1. Diligence
2. Knowing who is responsible
3. Knowing your risk
4. Practicing GMP
(good microbiological practice)
Chancellor
Senate
(Academic Issues)
Board of Governors
(Governance /
Management Issues)
President & Vice-Chancellor
Administration Ctte
Vice-President Academic
and Provost
Faculties - Deans
Vice- President Research
(PHAC and CFIA
Applicant Authority)
Assoc. V. P. Research
(Delegate of V.P R.
& Chair BSC)
Vice- President Resource
B/G Ctte – Health Safety,
& Environment
Biosafety Committee
( Assoc. VP Research,
Deans, Director ORM, &
BSO-Corp.
ORM - Director
LEGEND:
Chairs, Principal
Investigators, & Professors –
(BMUC Holders)
BSO– Corp.
(PHAC/ CFIA
Lead Contact)
ORM- BSO – Corp.
( Assist. Director
Radiation and Biosafety)
Corporate Governance
Academic
Research
ORM – BSO-Op.
(Biosafety Specialist)
Management
Your faculty, your colleague, PS,
PRS, HR ...
Federal & Provincial Agencies
Municipalities
International
Funding Agencies
So let’s get a hand on this !
Pathogenic………………..Non-pathogenic
Naturally occurring……..Manipulated
Ubiquitous………………..Evolving/Rare
It’s as easy as: 1 -2-3-4 !







Pathogencity
Infectious dose
Mode of transmission
Survival outside host and host range
Communicability
Immunization
Prophylaxis / Treatment
WHMIS
Class D, division 3 of WHMIS
(Poisonous and Infectious Material - Biohazardous
Infectious Material)
RISK GROUPS:
Risk Group
1
Individual
Low
Community
Implications
Low
Unlikely to cause disease in
healthy workers or animals
Rarely cause serious human
or animal disease
2
Moderate
Limited
3
High
Low
4
High
High
May cause serious disease
Likely to cause very serious
disease
Mammalian Cell Lines
Untransformed mammalian
cell lines - Risk Group 1
 MCF-7 (Human breast
carcinoma cell line)
 NIH 3T3 (Mouse
fibroblast cell line)
Transformed mammalian cell
lines – Risk Group 2
 HeLa (Human - contains
papovavirus)
Animal (may be infectious
without your knowledge, or
were intentionally injected
with a pathogen
Recombinant DNA
In vitro incorporation of
genetic material from one
cell into another or from
one organism to another
Level of risk depends on:
• the source of DNA being
transferred
• the vector
• the host
PRIONS
Includes unconventional agents,
slow viruses and prions causing
progressive neurological diseases
ex CJD, BSE, Scrapie
Resistant to destruction
Precautions:
 Handle tissues as Risk Group 2
or higher
 Handle formalin-fixed tissues
and paraffin-embedded blocks
as if still infectious
 Follow up-to-date disinfection
protocols.
Toxins
Endotoxins are part of the
outer membrane of the cell
wall of Gram-negative
bacteria.
Escherichia coli, Salmonella,
Shigella, Pseudomonas,
Neisseria, Haemophilus
influenzae, Bordetella
pertussis and Vibrio
cholerae.
Tetrodotoxin (TTX) is a potent
neurotoxin with no known
antidote.
CONTAINMENT LEVEL:

ROOM INTEGRITY





Laboratory locations &
access control
Air Handling and directional
air flow
Work Surfaces
Agents Used
Lab services (water, drains,
gas, electrical and safety)
( certification, commissioning)


measures required for
handling each organism
safely in a laboratory
setting
Specific to the risk group
level and amounts being
used
CL 1, 2, 3, or 4



First line of defence.
Ensures protection of personnel and immediate
environment from exposure to the infectious
agent.
‘Protective envelope’ that
infectious agent or animal.
Petri dish, vial
Biological safety cabinets
animal caging equipment
encapsulates
the


Protects the environment
external to the laboratory from
exposure
Includes facility design and
operational practices
Employs:
1. Directional airflow
2. Air and Drain filtration
3. HEPA Filtration of lab air
4. Pressure differentials
5. Laboratory Design
6. Operational Practices
Freezer
Incubator
Sink
Fumehood
Coats
office
Air
intake
4 BSC
Dead air

Basic laboratory

Requires no special design features

Biosafety cabinets are not required
and work may be performed on the
open bench.

Clinical, diagnostic, research and teaching
facilities with level 2 agents.

May require a class I or class II biological safety
cabinet

Emergency plan

Access controlled
Containment Level 3

Specialized design and construction including
commissioning and annual certification

Research projects reviewed by a specific panel

Standard operating procedures enforced for the safety of
the individual and proper operation of the lab.

Personnel – additional training and supervision.
Canadian Centre for Human
and Animal Health in
Winnipeg, Man.

Design specifications are
extremely stringent

The worker is completely isolated
from infectious material.

personnel security clearance and
qualifications scrutinized
Only 20% can identify the cause or event


80% are caused by human errors
20% are caused by equipment failure
Types of accidents causing LAIs
 Spills and sprays
 Needles
Vaccinia virus
 Sharp objects and broken glass
 Bites or scratches from animals
Pseudomonas
Attenuated – Lab Adapted Strains
Laboratory-Acquired Infection With an
Attenuated Yersinia pestis Strain—Chicago,
Illinois, 2009
1
2
3
4
5
• Agent characteristics & biological material
• Personnel supervising & personnel using the material
• Environment: laboratory, facility, community
• Experimental protocols & lab practices equipment
• Equipment
Agent characteristics & biological material

Is this a material you have used before?

Do you know the source and whether it has been tested for
which agents and to prove it is non-replicating

What are the characteristics of the material upon receipt?

What are the implications of the manipulations you are
planning?

Is this a material for which LAI have been reported or are
materials of concern ( gov’t, society, etc)?
SOURCE OF INFECTION
• Microorganisms
• Cells and tissues
• Blood and body fluids
• Any items contaminated with the above
Laboratory
Associated
Infection
SOURCE
HOST
SUSCEPTIBLE HOST
• Immune system
• Vaccination status
• Age
ROUTE
ROUTE OF TRANSMISSION
• Percutaneous inoculations
• Inhalation of aerosols
• Contact of mucous membranes
• Ingestion
Semen
Vaginal Secretions
Tissue Cultures
Organ Cultures
Infected Experimental Animals
Other Bodily Fluids:
Cerebrospinal, Amniotic, Synovial
 Pathogen involved
 Type of body fluid
 Route of exposure
 Duration of exposure
 Volume of blood involved in exposure
 Concentration of virus at time of exposure
 PPE worn
ONLY IN CANADA
Internationally
Recognized Resource
Designed, Research
And Maintained By
PHAC And CFIA
PATHOGEN MATERIAL
SAFETY DATA SHEETS
http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/index-eng.php
Personnel supervising & personnel using the material
• your knowledge and experience
• the level of mentorship available
• New User Registration Form &
Biosafety Health Assessment Forms
• records your knowledge level
• what you work with
• health status
Helps identify risk
factors and
potential for LAIs

Criteria for consideration
 Routes of exposure that need to
be blocked
 Degree of protection offered
 Ease of use
Only effective if
 correctly selected, fitted, used
and cared for
 the individual is well trained


Ensure PPE is removed before
leaving the lab
Gloves
- yes they are mandatory

Double gloving a good practice

Gloves should not be reused

Herpes simplex virus (HSV)
Gloves should be changed frequently

Glove selection: latex, nitrile, rubber & vinyl

Use the correct donning and doffing technique
Lab Coats/Gowns

Protect street clothing from spills

Offer additional body protection

Periodic cleaning required
Echo virus type 9
Eyewear – need I say more !
NO CONTACTS !
Eye glasses, goggles, facemask
Epstein-Barr Virus Infections
Facemask – prevent
inhalation
How many agents do you know
that have flu-like symptoms.
Influenza Virus
Footwear
 Closed toe and heel shoes
only.
 No sandals!
Streptococci bacteria
Minimum standard of practice for preventing the
transmission of BBP includes:
Education
Hand washing
Use safe work practice
Wearing appropriate protective equipment
If samples cannot be guaranteed non-infective
…… treat as infectious!
Environment: laboratory, facility, community
Regulatory
RequirementS
Lab
Environ
PHAC, CFIA, EC,
Fed/Prov/Munipcial)
Lab Design
(conception,
construction,
renovations,
maintenance)
Contain your
biohazard
(Primary and
secondary
containment)
uO
Dept
Control Access
Fac
(Physical controls:
lab and inventory)
• Experimental Protocols & Lab Practices Equipment
Experimental Protocols
• have to be researched thoroughly
• designed with safety in mind as well as research
• engage the supervisor
• protocols on-line: http://www.protocol-online.org
Remember once you start the protocol
you are in research mode,
so you better have thought of safety first!
Lab Practices
Good Lab Practices can save your life !
So adopt then from the start RG 1 or 2 or 3
The practices should change and the level of
consciousness you exhibit should as well.
PPE
Set up
Experimental
Decontamination
Waste
Leaving the lab
 What material is presently being used and/or
stored
 Location
 Expiry date
 Use log book
 MSDS’s
 Mandatory
What samples are
critical to save if
storage fails, and
have I identified
them?
FINALLY WE CAN START
TALKING LAB SPECIFICS
TRANSPORTATION OF
DANGEROUS GOODS
• Infectious substances
•Diagnostic samples
Yes there is
more training
you have to go
to.
IT’S THE LAW



Transportation of Dangerous Goods Act: Class
6.2 (Infectious Substances)
PHAC/CFIA restrictions
Ensure:
Proper classification
Proper packaging
Proper labeling
Proper documentation
Import/Export Permits

Pre-approved

Authorized Individuals

Lead time (International Regulations….)

Appropriate Scheduling (Holidays, Weekends)

Transportation within the building

Between lab to lab

Colleague to Colleague

Between Institutions
Important Considerations:
does material need to be transported at all
packaging requirements
means and route of transportation
regulatory requirements

Between lab transfers - 4 sided cart, sealed
primary container, secondary container, low
traffic route.

Off Campus transfers – consult ORM
At Shipping & Receiving

Verify shipment is yours, and expected.

Inspect the integrity of the outer container, to identify if
possible damage may have been incurred.

If no damage suspected, transfer to lab using appropriate
practices.

Open outer packaging (note this may require the use of a
biological safety cabinet if the risk group requires it.)
Package appears damaged
thank goodness for tdg my life just got a hole lot easier !

If damage and breakage possible, get your spill kit for your
biological sample ( Isn’t it handy that you did a risk
assessment!)

transfer the package into a secondary container lined with
absorbent paper (absorbent side up), close lid of container

Transfer to a cart with 4 sides for transfer to lab.
•Open in an appropriate fashion; inspecting for leakage,
breaks etc
•Decontaminate all the areas potentially contaminated.
•Dispose of sample in the appropriate manner
•Package must be sterilized, defaced prior to disposal.
REPORTING: If sample breach containment, inform your
supervisor, ORM (x. 3153), and PS
Before starting any manipulations
Before leaving the lab
Whenever the integrity of your gloves is
questioned or your hands are obviously soiled
Before and after completing any task in a BSC
Every time gloves are removed
Before contact with one’s face or mouth
At the end of the day
FREQUENTLY
– ABC
Aseptic technique is a set of specific practices and procedures
performed under carefully controlled conditions with the goal of
minimizing contamination by pathogens.
S
Space and work flow?
C
Clean, aseptic, or sterile technique?
R
I
Routine, aseptic or surgical hand hygiene?
Instruments and supplies?
Personal protective equipment?
P
Trash: sharps, infectious waste, radioactive
T
waste, pathology or routine waste?
a·sep·sis /āsepsis/
·
:
The absence of bacteria, viruses, and other microorganisms.
· The exclusion of bacteria and other microorganisms, typically
during surgery,
http://www.youtube.com/watch?v=4mKhULnxqcw

Avoid use whenever possible

Use a BSC for all operations with infectious material

Fill syringes carefully

Shield needles when withdrawing from stoppers

Do not bend, shear or recap needles.

Dispose of all used needles/syringes in yellow SHARPS
containersharps containers

Mouth pipetting is prohibited.

Never force fluids out.

To avoid splashes, discharge the liquid down the receiving
container wall.

Never mix material by suction and expulsion.

Reusable pipettes should be placed horizontally in a
disinfectant-filled pan.

Sterilization in an open flame may create
aerosols which may contain viable
microorganisms.

Shorter handles minimize vibrations

Disposable plastic loops are good alternatives
Flaming produces
aerosols so why
do it?
• Equipment
Use it &
maintain it
properly,
your life
depends on it !

Before use
 Check centrifuge rotors &
tubes for cracks
 Avoid Overfilling
 Place caps or stoppers properly
 Balance loads
 Use sealed buckets (safety cups) or sealed rotors


Before leaving: ensure centrifuge achieves run conditions
After run
 Centrifuge has to be completely stopped before opening the
lid
 Check for spills or leaks before removing samples. Clean spills
 Allow aerosols to settle (30 min) or open in a BSC

Operate in a BSC whenever possible. Allow aerosols to
settle for 5 minutes before opening.

Decontaminate after use
Blender
 Do not use glass blender jars
 Use safety blenders which can be autoclaved
Lyophilizers (used for dehydration process)
 Use glassware designed for vacuum work, ensure there
is no damage before using
 Use vapour traps whenever possible
Cryostats, Nitrogen Storage
Vessels, - 80 °C Freezers
Cryostats:
Wear gloves during preparation
of frozen sections and heavy
gloves when accessing the
cryostat. Decontaminate
frequently.
contain HEPA filters which
remove particles (min 0.3
microns) from supply and
exhaust air with 99.97%
efficiency .
Vertical or horizontal laminar
flow
HEPA filtered supply air only
Provide product protection
only
HEPA filtered supply and
exhaust air
Personnel and environment
Biological
Safety
Cabinet
protection
 3 Class II + III
http://www.youtube.com/watch?v=Wg61LdngWlQ
Before using the cabinet:
 Ensure BSC is certified
 Disinfect work surfaces with appropriate
disinfectant
 Turn off UV lamp; turn on fluorescent lamp
 Place essential items inside cabinet be prepared to
work from Clean to Dirty
 Allow the blower to run for 5-10 min before work
Hand movements when
entering, within and
exiting BSC must be
slow and deliberate to
prevent disturbing air
flow
Ensure:
 material and equipment is placed near the back of the
hood, especially aerosol-generating equipment.
 Do not block any vents
 Use techniques that reduce splatter
and aerosols.
After using the cabinet:
 Leave blower on at least 5 minutes to purge
cabinet
 Remove and decontaminate equipment and
materials
 Disinfect cabinet surfaces
 Turn off blower and fluorescent lamp, turn on UV
lamp
Maintenance:
 Before and after each use - Wipe down work surfaces
 Weekly
- Clean UV lamp
 Monthly - Wipe down all vertical surfaces
 Annually – Verify UV lamp intensity
- Decontamination with formaldehyde gas
(managed by ORM x 3153)
 Certification – is required if the cabinet was moved that
as filter seal could have been breached
(contact ORM x 3153)
DECONTAMINATION ?
DISINFECTION ?
STERILIZATION ?

Decontamination:

The destruction of microorganisms to a lower level
such that it removes danger of infection to
individuals.

Sterilization:

The complete destruction of all viable
microorganisms.

Disinfection:

Use of agents (physical or chemical) to destroy
harmful organisms on inanimate objects

Heat:
Autoclaving (most practical and
recommended)
Incineration (for disposal of sharps and
tissues)

Irradiation:
UV light (wavelength of 253 nm is germicidal)
Gamma (disrupts DNA and RNA)

Filtration
HEPA (biological safety cabinets, ventilation)
Items that CAN be autoclaved:

Culture dishes and related devices

Cultures and stocks of infectious material

Discarded live and attenuated vaccines

Contaminated solid items (petri dishes,
eppendorf tips, pipettes, gloves, paper towels)
Items that CANNOT be autoclaved:

chemicals (flammables, oxidizers, phenols, acids,
alkalides)

chemotherapeutic or radioactive waste

bleach (or other chlorinated products)

certain kinds of plastics

Sharps (not at the University of Ottawa)

Many autoclaves are now run by dedicated staff,
however, if you are operating an autoclave:
Learn how to use it!
Ensure PPE is worn
Recognize acceptable material and packaging
Proper loading and unloading

All users/operators must take autoclave training
http://www.benchfly.com/video/139/using-an-autoclave/
Preparation of waste:

Use only approved autoclave bags

Do not overfill autoclave bags

Separate material for re-use from that which will
be disposed, and dry from liquid material

If outside of bag is contaminated, double bag

All flasks containing biological material should
be capped with aluminum foil

Ensure items are labeled with contact
information

Generally for disinfection rather than
sterilization

Choice depends on:
Type of material to be disinfected
Organic load
Chemical characteristics

Most common are chlorine compounds and
alcohols (broad range)
Vegetative bacteria (E.coli,




Staph)
2% domestic bleach
75% Ethanol
Quaternary ammonia
6% formulated Hydrogen peroxide
Mycobacteria and fungi



10% domestic bleach
75% Ethanol
Phenolic compounds
Spore forming bacteria




(Bacillus)
10% domestic bleach
Glutaraldehyde
Formaldehyde
6% formulated Hydrogen peroxide
Viruses
Enveloped




(HIV, Herpes)
2% domestic bleach
75% Ethanol
Quaternary ammonia
6% formulated Hydrogen peroxide*
Non enveloped (Hepatitis,
Adenovirus)




10% domestic bleach
6% formulated Hydrogen peroxide*
Glutaraldehyde
Formaldehyde
Discarded biological material from teaching, clinical
and research laboratories and operations is
biomedical waste.
Includes but is not limited to:
 Animal waste
 Biological laboratory waste
 Human anatomical waste
 Human blood and body fluid waste
 Sharps

All biological waste should be decontaminated
prior to disposal (including level 1 agents).

Treated waste is no longer considered ‘biomedical’
(i.e. microbiological waste, blood and bodily fluid
waste) and can be disposed of in the regular waste
stream.

Any waste that cannot be treated (i.e. sharps,
carcasses, tissues and body parts) remains
biomedical waste and must be incinerated off site.
Biomedical Waste (treated)
*in compliance with
sewer use by-laws
with H2O (1:10)
Biomedical Waste (untreated)
YOU TELL ME .....
Mitigates the risk of:
1. Personnel exposure
2. Contamination
a) sample
b) environment
It’s you or them,
make your decision!
It’s you or it’s them, make a choice!
What are we talking about ?


Are you
prepared?
Did you
anticipate
this?
I told you that
risk assessment
would come in
handy!
◦ What is the risk?
◦ What is the route of exposure?
◦ Are aerosols still suspended?
◦ Is the risk contained?
REMEMBER – if the risk was
inhalation, there may not be
any evidence of an exposure
having occurred.


Inform all those in the vicinity.
Restrict access and resuspending or relocation
of particles.

Vacate area for 30 minutes before re-entering.

Report, sign area, seek medical assistance.
Spill response will vary depending on:
what, where, how much, when, who


Cover spill area with absorbent material
Soak the spill area with an appropriate
disinfectant (i.e. 10% bleach, Virox)

Pour disinfectant from the outside of the
absorbent material towards the inside

Leave on for 20 to 30 minutes

Pick up any broken glass (with forceps!) and
place in a SHARPS container

Wipe up with absorbent material

Waste should be disposed in appropriate
biohazardous waste container
UO template is available:
(http://www.uottawa.ca/services/ehss/docs/SPILL
RESPONSEPLAN.pdf)
Don’t forget it must be tailored to be lab specific.
All potential exposures
immediately to:
 Your
supervisor /PI
 ORM
x 5892
 5411
(through Protection
Services)
No Excuses!
 Occupational
Health,
Disability and Leave Form
On-line
https://web30.uottawa.ca/v3/riskmgmtfrm/aioreport.aspx?lang=en
YOU WANT FUNDING FAST –
HERE ARE SOME TRICKS

Purchasing & Receiving of Biological
Agents
 PHAC, CFIA, Environment
Canada
 Inventory Records

Transportation/Transfer
 Transport Canada- TDG
Tricouncil,
Funding Agencies , Contracts (MTA)
Require compliance to the established biosafety program
(based on federal, provincial, municipal and international
requirements.
Project Spec Form ( Project Review)
New User Registration Form
Training
Streamlining them, On-line migration
But they must be complete in that they must be detailed
EFFORT = RESULTS
Delays are a result of incomplete submissions



Project specific form (Tricouncil requires biosafety review)
Describe what you are doing as it relates to your awarded
grant
What agents you will use ---- NO SHORT CUTS! It has to be
what is described in your grant proposal or MTA
ASK YOURSELF
Would my answers pass a Funding Agency AUDIT ?



Biohazardous Material User Registration Form
It incorporates: training, experience,
proposed work details
Biosafey Health Assessment Form ( it is for
your own safety)
“How soon do you need it?” “You want it when?”
In order to facilitate a quick turnaround, provide:
 reference to which grant
 copies of MSDS’s
 reference documents
REMEMBER: Suppliers may need PHAC / CFIA
Importation permits or letters of compliance.
*Restrictions may exist
Material Transfer Agreement conditions
Existing Import Permit conditions
*Permits required
(PHAC, CFIA (animal, plant, aquatic), DFAIT)
*Facility certification
*Transportation of Dangerous Goods
*International Holidays
Time factors:
 complete
submission
 gov’t turn
around (2-4 wk)
 Lead time
 Grant cycle
http://www.uottawa.ca/services/ehss.biosafety.htm
Biohazardous Materials
User Registration
Biosafety Health
Assessment Survey
UOttawa webpage
Services
ORM
Programs (left column)
Biosafety
You haven’t finished
training until you
take the training
comprehension
test.
Access to test on Virtual Campus is granted by
Biosafety Compliance Specialist, requires
providing: Name, email, employee/student #
It’s for your own
safety!
CONFUSED ?
OVERWHELMED ?
QUESTIONS ?
Lois Sowden-Plunkett
lsowden@uottawa.ca
Ext. 3058
Pierre Laflamme
plaflam2@uottawa.ca
Ext. 3153