DEVELOPING OF INTEGRATED FARMING’S MODEL TO MAINTAIN THE GREEN BIOTECHNOLOGY NI NYOMAN TRI PUSPANINGSIH MIRNI LAMID ENDANG SRI MASITHAH AIRLANGGA UNIVERSITY : AUTONOMY UNIVERSITY, EXCELLENCE WITH MORALITY ENZYME RESEARCH ON : PECTINASE HEMICELLULASES CELLULASE PLANT CELL WALL EXCELZYME Why enzymes? Advantages • Natural, Nontoxic • Catalyze specific reactions • Active under mild conditions • Active at low concentrations • Can control rate of reaction • Can be inactivated EXCELZYME TOWARDS GREEN TECHNOLOGY • EXCELZYME 1: REDUCING OF 30-40% CHEMICAL COMPOUNDS BIO-DEINKING/BIO-BLEACHING ON PULP AND PAPER INDUSTRY • EXCELZYME 2 : NUTRIENT ENHANCEMENT OF AGROINDUSTRIAL WASTE AS RUMINANTS FEED FORMULATING FEED ENZYME IN COMPLETE FEED EXCELZYME • EXCELZYME 3 : REDUCED ENVIRONMENTAL IMPACT OPTIMIZE THE COMPOST PROCESS BASED ON LIGNOCELLULOSE MATERIAL AND ANIMAL WASTE FOR BIO-FERTILIZERS (ORGANIK AND PLANKTON FERTILIZERS) • EXCELZYME 4 : REDUCED ENVIRONMENTAL IMPACT OPTIMIZE EXCELZYME 4 AS PREBIOTIC FOR REDUCING BLOOMING MICROCYSTIS INTEGRATED FARMING’S MODEL G Low Quality Lignocellulose Biomass Increasing Crops L 0 B Excelzyme treatment Efficient Technology Agriculture and Fish Pond A L W High Quality of Ruminant and Poultry Feed Organic and Plankton Fertilizers A R M Source of animal protein Increasing of animal weight I Animal Waste + Biomass N Excelzyme treatment G ANALYSIS EXCELZYME ACTIVITIES A. Excelzyme 4 : Reducing Blooming Microsystis before After + Excelzyme 4 B. Excelzyme 3 : Indicating hemicelulase activity Lignocellulose + Excellzyme3 Lignocellulose + Excellzyme3 + H2O2 EXCELZYME APPLICATION ORGANIC FERTILIZERS FISHERIES APPLICATION 1. PLANKTON FERTILIZER 2. REDUCING BLOOMING MICROCYSTIS GROWING THE PLANKTON FERTILIZER BLOOMING MICROCYSTIS ANIMAL FEED ENZYME USING RICE STRAW NUTRITION CONTENTS RICE STRAW RICE TRAW + EXCELZYME DRY MATERIAL (%) 95,39 92,37 CRUDE FIBER (%) 33,83 24,14 CRUDE PROTEIN (%) 5,46 7,47 ASH ((%) 20,43 20,33 ORGANIC MATERIAL (%) 74,96 72,03 ENZYMES FOR DOMESTIC DEMAND Lignocellulosic waste : 169 million ton/year Agrobusiness Demand (ton/year) (in million) Enzyme ton/year (in million) Average Price/kg (USD) import Animal feed 7.6 1.5 10-15 Fertilizer 0.9 0.09 8-10 9 1.8 14-20 Pulp/Paper Acknowledgement International Research Group Collaboration on Lignocellulase Enzymes of Airlangga University with : 1. University of Groningen, The Netherlands 2. Mie University, Japan 3. Universiti Putra Malaysia, malaysia 4. Universiti Kebangsaan Malaysia, Malaysia 5. Universiti Teknologi Malaysia, Penang, Malaysia The activities : Joint Research, Joint Supervisors, Student Exchange, Joint Publication Collaboration with Indonesian industries : • PT Bumiindo (Bio-energy) • PT Pupuk Kaltim (Bio-Fertilizer) • PT Siklus Alam Semesta (Bio-Fertilizer) • PT Adi Prima (Paper and Pulp Industry) • PT Eka Timur Raya (Bio-Fertilizer) THANK YOU