Lab #15 Blood What are the parts of the blood? What are some diseases that affect human blood? TASK #1 OBSERVING RED BLOOD CELLS, WHITE BLOOD CELLS AND PLATELETS Human Blood Parts Red Blood Cells (Erythrocytes = scientific name) –Carry oxygen (gas) –Protein = Hemoglobin – helps them hold on to the oxygen –Most numerous in a blood smear Red Blood Cells 1. Draw one or two RBC’s on your chart on page 2 where it says drawings and labels 2. Label • Cell membrane • Cytoplasm 3. Number of cells in field (count) = 100 Cell membrane Cytoplasm Human Blood Parts White Blood Cells (Leukocytes = scientific name) – Function in the immune system – Much larger than RBC’s – Least Numerous – Surround and engulf bacteria = phagocytosis – Since they help fight infections a high number of them may indicate that you are sick and/or have a disease! White Blood Cells 1. Draw one or two WBC’s on your chart on page 2 where it says drawings and labels 2. Label: • Cell membrane • Cytoplasm • Nucleus • Number of cells in field (count) = 15 Cell membrane Nuclear material This is it!! Cytoplasm Human Blood Parts Platelets (Thrombocyte = scientific name) –Function in clotting –“Dot-like” fragments scattered throughout smear –Cell fragments, not cells! –Next most numerous Platelets 1. Draw some platelets on your chart on page 2 where it says drawings and labels. 1. You DO NOT have to label anything for these. 2. They are the little purple dots!!! 3. “t” stands for thrombocyte 2. Number of cells in field (count) = 50 Human Blood Parts Plasma – Functions in transporting nutrients and hormones. – Separates this way due to density. – Yellowish in color 1. On your lab, sketch the test tube showing the separation of blood 2. Label the parts of the blood as shown in the diagram TASK #2 Observing a healthy human blood smear Human Blood Smear White blood cell Magnification 800x Identify: 1. Red Blood cell • Label cell membrane • Label cytoplasm 2. WBC • Label cell membrane • Label cytoplasm • Label nucleus 3. Platelets TASK #3 Observing common blood diseases and disorders Leukemia Smear Diseased white blood cell Magnification 800x Identify: 1. Red Blood cell • Label cell membrane • Label cytoplasm 2. WBC • NOTICE THAT THE NUCLEUS IS MUCH BIGGER! • Label cell membrane • Label cytoplasm • Label nucleus 3. Platelets SICKLE CELL ANEMIA Identify: 1. Red Blood cell • Label cell membrane • Label cytoplasm 2. Sickle cell 3. WBC • Label cell mem • Label cytoplasm • Label nucleus 4. Platelets Magnification 800x Mononucleosis Smear Identify: 1. Red Blood cell • Label cell membrane • Label cytoplasm 2. WBC • NOTICE THE WATERY APPEARANCE OF THE WBC • Label cell membrane • Label cytoplasm • Label nucleus Magnification 800x 3. Platelets TASK #4 Observing the blood of other vertebrates Frog Blood Platelet Identify: 1. Red Blood cell • Label cell membrane • Label cytoplasm • Label nucleus 2. WBC • Label cell mem • Label cytoplasm • Label nucleus 3. Platelets Bird blood Red blood cell White blood cell Identify: 1. Red Blood cell • Label cell membrane • Label cytoplasm • Label nucleus 2. WBC • Label cell mem • Label cytoplasm • Label nucleus 3. Platelets • Just draw some dots!