SBI4U: Homeostasis
Name:_________________
DAPHNIA LAB EVALUATION 2015
Name: _____________________
Partner: ______________
Due Date: Thurs. Mar 5, 2015
Variables Identified
Materials
Safety Notes and Prep Questions
Observation Table Design
Procedure
Original Data Observations and averages for your team, attached
Whole Class Data Observations and averages printed, attached
Conclusions
For each drug compare class average for drug to control average
and explain results as expected or unexpected and give a reason
why, use data from the lab!
- nicotine
- caffeine
- Aspirin
- Alcohol
- lactic acid
- adrenaline
Write all conclusions in complete sentences.
Describe 2 Sources of Error, complete sentences
Spelling, Grammar, Correct Report Format
Organized and handed in with all parts, on time, complete
Write all conclusions in complete sentences
TOTAL
Daphnia Drug Lab Follow-up Questions
0123
01
0123
0123
012
0123
012
012
012
012
012
012
012
012
01234
/35
/8
Lab Performance
/10
- come prepared, know what to do, act safely, avoid bringing backpacks, treat Daphnia
respectfully, record data accurately, stay on task and organized
SBI4U: Homeostasis
Name:_________________
Self-Directed Daphnia Lab Preparation 2015
In this lab you will take the initiative to prepare a lab by yourself. Before you perform this lab
you must have written up the following:
1. Variables – Identify the independent, dependent and controlled variables for the control and
one drug sample.
2. Purpose
3. Hypothesis – 6 sentences, 1 per drug, predict the effect of the drug on the daphnia
Lactic acid, caffeine, aspirin, alcohol, nicotine, adrenaline
4. Materials – 2 column list
5. Safety – make a small chart of the personal safety hazards and precautions for using these
drugs on the daphnia. MSDS are in the back of the drawer.
6. Make observation charts for the required data.
7. Make your own procedure. You and your partner MUST know how to perform the lab
efficiently. It is up to you to work this out.
NOTE: Counting the heartbeats is a challenge and you must work as a team to do this part of
the lab. One person will observe and tap a pencil for each heartbeat while the partner will
slowly pull the paper up so the dots are countable. Do watch the videos on our website.
During the Lab
1. Safety – gloves (optional), microscope light gets hot
2. Each person in a partnership will have a microscope and try to conduct their own research.
You will help your partner when they are ready for timing and counting.
3. To get the most data the first partner will do the control and then the seond partner will
start with a drug, then the next drug and the next until all the data is gathered.
a) lactic acid
b) aspirin
c) nicotine
d) caffeine
e) adrenaline
f) alcohol
4. Never ever put your pipettes in the stock supply of anything!! Pour your own sample of
distilled water into a labelled medicine cup.
5. Each person should have their own pipette and “detox centre” with distilled water in a
medicine cup.
6. Record all heartbeat data on the supplied scrap paper. Be sure to label your name and the
drug. Keep this original data and hand in with your lab.
7. Do 2 trials of 30 sec each. Calculate the average heartbeats per minute.
(Simplified math: Average beats per minute = [trial 1 +trial 2]) Record this on the spreadsheet
before you leave class.
8. Class data will be available on line by noon on _______________________. Lab write-up will
be based on the average of the averages for the control and each drug.
9. Be sure you read the evaluation scheme to direct your lab conclusions!!!
SBI4U: Homeostasis
Name:_________________
DAPHNIA DRUG LAB FOLLOWUP – DRY LAB ANALYSIS
An experiment was performed to determine the effect of a drug, labelled X, on human metabolism. The
rate of metabolism can be measured indirectly by monitoring changes in body temperature.
Procedure
1. Four comparable groups consisting of 50 individuals per group were used in the experiment. Each
group had 25 healthy males and 25 healthy females between 40 and 50 years of age.
2. At the same time each day, all group members were given a dosage of the drug, except for group 4,
which was given a placebo that did not contain the drug.
3. Each group member was monitored for changes in body temperature and in the volume of secretion
from the thyroid gland.
Data
Group #
Dosage of drug X
( x 10 g / 50 kg of body mass)
-6
1
2
3
4
1
10
100
placebo
Change in body
temperature
( C)
+0.2
+0.9
+1.2
0.0
Perspiration
slight increase
moderate increase
large increase
no change
a)
Identify the independent variable for this experiment. [1]
b)
Identify the dependent variable for this experiment. [1]
c)
What is the control sample? Explain. [2]
d)
What are two controlled variables for this experiment? [2]
e)
Using this data, does the drug increase metabolic rate? Explain using evidence from the
experimental results. [2]
SBI4U: Homeostasis
Name:_________________
Daphnia Anatomy
http://lanwebs.lander.edu/faculty/rsfox/invertebrates/daphnia.html