should be dark purple
Will be acidophilic or stain with eosin/can be better visualized with an EM
should be dark purple
Euchromatin is not stainable with H&E, loosely packed inactive DNA
Heterochromatin is basophilic w/a LM, dark and tightly packed DNA
Dark pink
Not stained with an H&E section
Not stained
Cannot be seen
Can create a blue color in the cytoplasm of some cells
basophilic staining
basophilic staining
Assists in the breakdown of materials inside a cell, sometimes creating the "wear and
tear" pigment lipofuscin when there is autolysis
* HALLS STAIN IS FOR BILE
Melanin, hemosiderin, lipofuscin, bilirubin
Asbestos, tattoo ink, rust, talcum powder, carbon, dusts, and metals
bond between opposite charges
when atoms share electrons
when a covalently bonded hydrogen is connected to atoms such as oxygen or nitrogen
staining with basic dyes and staining with dyes with metal
The polymer chains contain terminal amino acid groups NH2 and COOH are amphoteric
and can be positive or negative depending on pH
likes basic areas on tissue/cell
likes acidic areas of the cell/tissue
property of color, tells it what the color is going to be
benzene compound containing chromophore, actual group determining the color of dye
ionizing group allowing dye to link to the tissue and influences color intensity
most common auxochrome are amino, carboxyl, sulfonic, and hydroxyl
pH through establishing charges within the tissue and the dye
increase of temperature will increase the staining rate
concentration increases dye molecules
salts may increase or decrease staining rate PROBABLY because ions are competing for binding sites
Fixatives may bind to staining sites and make them unavailable for dyes
Progressive - staining and stopping without overstaining.
Regressive - Overstain and then differentiate.
substance/metal that is a link between tissue and dye.
Regressive. Hematoxylin, absolute ethyl alcohol. ammonium aluminum sulfate, distilled water, sodium iodate (mercuric
chloride not used anymore)
Oxidizer is sodium iodate
Mordant is ammonium aluminum sulfate
Regressive. Ammonium aluminum sulfate, distilled water, hematoxylin, alcohol, glycerol, ethyl alcohol
Oxidizer is Sunlight/Oxygen
Mordant is Ammonium Aluminum Sulfate
Progressive. Hematoxylin, distilled water, sodium iodate, ammonium or potassium aluminum sulfate, citric acid, chloral
hydrate
Oxidizer is sodium iodate
Mordant is ammonium or potassium aluminum sulfate
May be used progressively but more commonly regressive. Hematoxylin, 95% alcohol, distilled water, glycerol,
ammonium or potassium aluminum sulfate, glacial acetic acid
Oxidizer is Sunlight/Oxygen
Mordant is ammonium or potassium aluminum sulfate
Progressive for 1&2 because they do not overstain. Progressive is used for Gill 3 as well. Distilled water, ethylene
glycol, hematoxylin, sodium iodate, aluminum sulfate, glacial acetic acid.
Oxidizer is sodium iodate
Mordant is aluminum sulfate