Notes on 2.2 Bacterial Cells Bacterial Cell Structure: • Bacterial cells, or prokaryotic cells, are distinct from animal and plant cells (eukaryotic cells). • Cell Wall: Unlike plant cell walls which are made of cellulose, bacterial cell walls serve a similar function in providing support and preventing the cell from bursting under osmotic pressure but are made from different materials (like peptidoglycan in many bacteria). • Cell Membrane: Just like in other cells, the bacterial cell membrane is partially permeable, controlling the influx and efflux of substances. • Cytoplasm: Contains all the enzymes and substances necessary for metabolic reactions, excluding mitochondria or chloroplasts. • Ribosomes: Present for protein synthesis, similar to those in eukaryotic cells but slightly smaller (70S ribosomes). Genetic Material: • Nucleoid: Bacteria do not have a nucleus; instead, their DNA is organized in a circular chromosome located in a region called the nucleoid. • Plasmids: Bacteria may also contain plasmids, which are small rings of DNA that can be used in genetic engineering. Reproduction: • Bacteria reproduce through binary fission, a form of asexual reproduction where one cell divides into two identical cells, as seen in the electron microscope image of Enterococcus faecalis. Comparison with Eukaryotic Cells: • Nucleus: Absent in bacteria, which distinguishes them fundamentally from plant and animal cells. • Organelles: Lack membrane-bound organelles like mitochondria and chloroplasts. • Size: Generally smaller than eukaryotic cells. Multiple Choice Questions 1. Which component is unique to bacterial cells compared to plant and animal cells? • A) Nucleus • B) Cell wall made of cellulose • C) Cell membrane • D) Circular DNA 2. What is the function of the cell wall in bacterial cells? • A) Photosynthesis • B) Protein synthesis • C) Protecting the cell and preventing bursting • D) Storing genetic information 3. Which of the following best describes bacterial ribosomes? • A) They are larger than those in eukaryotic cells. • B) They are the same as in plant cells. • C) They are smaller than those in eukaryotic cells. • D) They do not exist in bacterial cells. 4. Bacterial cells reproduce by: • A) Sexual reproduction • B) Binary fission • C) Budding • D) Spore formation 5. What are plasmids? • A) The main chromosomes of bacteria • B) Small, circular pieces of DNA used in genetic engineering • C) Organelles that produce energy • D) Structures that store nutrients True or False 6. Bacterial cells have a nucleus. (True/False) 7. Bacterial cell walls are made of cellulose. (True/False) 8. Cytoplasm in bacterial cells contains mitochondria. (True/False) 9. All bacteria have the same shape and size. (True/False) 10. Bacterial cells can contain more than one circular DNA molecule. (True/False) Short Answer Questions 11. Explain how the absence of a nucleus in bacterial cells affects their function and structure. 12. Describe the role of plasmids in bacterial cells and their importance in biotechnology. 13. Compare and contrast the cell walls of bacterial cells and plant cells. Diagram Labeling 14. Label the parts of a bacterial cell on a provided diagram. Include the cell wall, cell membrane, cytoplasm, ribosomes, and DNA. Answers to Exercises on Bacterial Cells Multiple Choice Questions 1. Which component is unique to bacterial cells compared to plant and animal cells? • D) Circular DNA 2. What is the function of the cell wall in bacterial cells? • C) Protecting the cell and preventing bursting 3. Which of the following best describes bacterial ribosomes? • C) They are smaller than those in eukaryotic cells. 4. Bacterial cells reproduce by: • B) Binary fission 5. What are plasmids? • B) Small, circular pieces of DNA used in genetic engineering True or False 6. Bacterial cells have a nucleus. (False) 7. Bacterial cell walls are made of cellulose. (False) 8. Cytoplasm in bacterial cells contains mitochondria. (False) 9. All bacteria have the same shape and size. (False) 10. Bacterial cells can contain more than one circular DNA molecule. (True) Short Answer Questions 11. Explain how the absence of a nucleus in bacterial cells affects their function and structure. • Bacterial cells lack a nucleus, meaning their DNA is not enclosed within a membrane but rather is free in the cytoplasm. This setup, termed prokaryotic, allows for quicker transcription and translation processes as there is no nuclear barrier. However, it also exposes the genetic material to more potential damage from environmental factors. 12. Describe the role of plasmids in bacterial cells and their importance in biotechnology. • Plasmids are small, circular DNA molecules that exist independently of the main bacterial chromosome. They often contain genes that can confer advantageous traits such as antibiotic resistance. In biotechnology, plasmids are used as vectors to insert new genes into bacteria, facilitating the production of proteins, vaccines, and other useful substances. 13. Compare and contrast the cell walls of bacterial cells and plant cells. • Bacterial cell walls are primarily composed of peptidoglycan, which is different from the cellulose-based cell walls of plant cells. While both provide structural support and protection, the chemical differences make bacterial walls a target for antibiotics, which disrupt wall synthesis. Diagram Labeling 14. Label the parts of a bacterial cell on a provided diagram. • Cell wall: Surrounds the cell, providing structural support and protection. • Cell membrane: Located just inside the cell wall, controls the passage of substances in and out of the cell. • Cytoplasm: The gel-like substance inside the cell containing all cell components and molecules. • Ribosomes: Tiny structures in the cytoplasm where proteins are synthesized. • DNA: Typically a single circular chromosome located in the cytoplasm, contains genetic information.