In this experiment, we aim to investigate the preventive effect of phytochemicals on the
progression of breast cancer promoted by Bisphenol A (BPA). We will employ a mouse
model, commonly used in cancer research due to its physiological and genetic similarity to
humans.
Mice will be divided into four groups: a control group, a group exposed to BPA, a group
treated with phytochemicals, and a combination group exposed to BPA and treated with
phytochemicals. BPA exposure will mimic human exposure routes, administered orally or
through injection. Phytochemical treatment will involve administration of compounds like
resveratrol, curcumin, or EGCG, known for their anti-cancer properties.
The experiment will involve careful monitoring of the mice for signs of breast cancer
development, including tumour formation and changes in behaviour or weight. At the end of
the study period, breast tissue samples will be collected for histological analysis to evaluate
tumour incidence, size, and molecular markers associated with cancer progression.
Expected outcomes include a higher incidence and faster progression of breast cancer in the
BPA exposure group compared to the control group. The phytochemical treatment group is
expected to exhibit reduced cancer incidence and slower progression compared to the BPA
exposure group. The combination group will reveal whether phytochemicals effectively
counteract the promotion of breast cancer by BPA.
Ethical guidelines will be followed throughout the experiment, and factors such as diet and
environmental conditions will be controlled. Statistical analysis will be conducted to compare
tumour parameters between groups, and the experiment will be repeated to ensure
reproducibility. Additional analyses like gene expression profiling may be performed to
deepen our understanding of the underlying mechanisms.
Overall, this experiment aims to provide valuable insights into the potential preventive role of
phytochemicals against BPA-promoted breast cancer progression, contributing to the
development of strategies for cancer prevention and intervention.