XANTHOPROTEIC TEST Requirements Of Xanthoproteic Test Instruments and Materials Test tubes Test tube stand Pipettes Dropper Bunsen burner Chemicals and Reagents 3 ml of test solution 40% of NaOH solution 1ml of conc. HNO3 Procedure Of Xanthoproteic Test Take a dry and sterile test tube. Add 2 ml of the given sample solution into the test tube with the help of dropper. Add 1 ml of concentrated sulfuric acid (HNO3). Boil the test solution gently on a Bunsen burner. The solution turns yellow; then cool the test solution. Add 2ml of 40% of NaOH solution till the solution turns alkaline. Observe the change in color. Observation And Inferences of Xanthoproteic Test Formation of a deep yellow or orange color gives this test positive.