Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija “The Effect of Banana (Musa acuminata) Peels and Leaves Extracts in the Growth of Pechay (Brassica rapa)” A Research Paper Presented to The Faculty and Staff of Senior High School Department Dr. Ramon De Santos National High School In Partial Fulfilment Of the Requirements In Research Project Presented By: Alessandra Noelle Mendoza Zammir Louie Constante Czarina Nicole Ocampo Bea Angeline Salvador Vanessa Abellar March 2023 Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija CHAPTER I THE PROBLEM AND ITS BACKGROUND Introduction One of the most important problems in the large area that has been utilized for agricultural production during the last years is the loss of soil fertility. One of the crucial factors that directly affect crop yield and quality is soil fertility and nutrient management (Nair, 2018). According to the Department of Primary Industry, the soil is a significant source of the nutrients such as potassium, phosphorus, and nitrogen that are required by plants for growth. In addition, the nutrients calcium, magnesium, and sulfur as well as iron, manganese, zinc, copper, boron, and molybdenum are also important for a plant to grow. This led to the rise of organic fertilizers comprising a variety of plant-derived materials that range from fresh or dried plant material to animal manures and litter to agricultural byproducts (Green, 2015). According to the Philippine Council for Agriculture, Forestry and Natural Resources Research and Development (PCARRD), The most economically significant fruit crop in the Philippines is the banana, which is also the only locally produced fruit that is always in season. Around 114 million metric tons of banana waste are produced globally, which causes environmental issues like the excessive emission of greenhouse gases. There are roughly 10 million hectares of banana plantations worldwide, and each one generates 220 tons of waste annually. Generally, the pseudo stem, leaves, inflorescence, fruit stalk (floral stalk/rachis), rhizome, and peels are the byproducts of the banana after it has been harvested. Most of these by-products could be used as undervalued commodities with little Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija market potential and, in some cases, as agricultural waste. In farms, the leaves are frequently left to rot (Padam et. Al., 2014). Banana peel is the outer shell (cover) of the banana fruit. It is a by-product of home consumption and the processing of bananas (National Library of Medicine, 2022). Additionally, Banana peel has been found to contain a variety of vitamins and minerals such as phosphorus, iron, calcium, magnesium, and sodium (Hikal et. Al, 2022). Banana leaves also consist of potassium, calcium, phosphorus, and magnesium (Barode, 2022). According to Brisbane City Council, banana leaves and banana peels is full of nutrients that can help the plants to grow. Pechay (Brassica napus l) belongs to the Brassicaceae family and is one of the most-known vegetables in the Philippines. It is an erect, biennial herb, cultivated as an annual about 15-30 cm tall in the vegetative stage. It grows easily under normal conditions, with or without fertilizers (Nacau, 2019). The general purpose of this study is to identify if an agricultural waste of bananas such as leaves and fruit peel affects the growth of Pechay (Brassica napus l). This study aims to benefit local vegetable farmers as well as banana vendors and produce alternative ways of creating new products and applications by recycling banana agricultural wastes. Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija Statement of the Problem Due to the large amount of agricultural waste of banana such as banana leaves and banana peels, thus this study aims to utilize other way to make use of this agricultural waste. Specifically, this study seeks an answer to the following questions: 1. Is there an effect of banana peels and banana leaves extract on the growth of pechay? 2. Is there a significant difference in the pechay in terms of height, number of leaves, and weight of pechay, compare to the control supplemented with the organic fertilizer? 3. Is there a significant difference in the growth of pechay in terms of height, number of leaves, and weight compared to the growth of pechay supplemented with commercial fertilizer? Hypothesis 1. Banana peel and banana leaf extract has no effect on the growth of pechay. 2. There is no significant difference in the pechay in terms of height, number of leaves, and weight of pechay, compare to the control supplemented with the organic fertilizer. 3. There is no significant difference in the growth of pechay in terms of height, number of leaves, and weight compared to the growth of pechay supplemented with commercial fertilizer. Dr. Ramon De Santos National High School Input San Antonio, Cuyapo, Nueva Ecija Conceptual Framework Input Collection of the Musa acuminata peels and leaves and Collection of Brassica rapa Process The Musa acuminata peels and leaves are extracted using water. Output Musa acuminata peels and leaves extract. Figure 1: Paradigm of the Study The input includes collection of the Musa acuminata peels and leaves and Collection of Brassica rapa for the production of the extract. The process includes the extraction of Musa acuminata peels and leaves. The output contains the result and analyzation obtained from the actual experimentation. Scope and Delimitations This study will focus on the effect of banana peel and banana leaves as an alternative fertilizer. The effect of banana peel and banana leaves extract will be evaluating its quality compared to available fertilizers in the market. Significance of the study This study will give information and ideas about "The Effect of Banana (Musa acuminata) Peel and Leaves in the Growth of Pechay (Brassica rapa)" Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija To the Community, the people in the community will have an affordable and useful alternative fertilizer for the growth of Pechay. To the Future Researcher, to get reliable information based on the results and to acquire or generate new knowledge on it. To the Farmers, to know the alternative, organic and affordable fertilizer for the growth of their crops. To the Farmers, to know the alternative, organic and affordable fertilizer for the growth of their crops. Definition of terms Banana leaves- the banana's skin (especially when it is stripped off and discarded) Banana peel- banana plant's leaf, which can grow up to 40 leaves at a time. Extract- the process of removing a substance from another substance. Pechay- is a vegetable plant with green leafy leaves, small in size, have white stalk of the leaves. Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija CHAPTER II REVIEW OF RELATED LITERATURE Related Literature Banana (Musa acuminata) Peels and Leaves It has been demonstrated that banana peel, a commonly found agricultural waste, is a valuable source of nutrients for plant development. Many researches have studied the usefulness of banana peel extract in boosting plant growth and development. Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija According to Girma et al. (2018) the researchers evaluated the influence of banana peel extract on the development of maize seedlings. When compared to the control group, they discovered that applying banana peel extract significantly increased plant height, leaf area, and biomass. The high levels of nitrogen, potassium, and phosphorus in the banana peel extract were cited by the researchers as the cause of this impact. In a study published in 2015 by Badgujar et al., the researchers also investigated at how banana peel extract affected the development and production of wheat plants. They discovered that the use of banana peel extracts increased grain production significantly and enhanced plant height, biomass, and chlorophyll content. The high levels of nitrogen, potassium, and phosphorus in banana leaves make them a great source of nutrients for plant development. Many research have studied the efficiency of banana leaf extracts in boosting plant development. In one study, Khan et al. (2016) examined how banana leaf extract affected the development of mung bean plants. The outcomes demonstrated that, in comparison to the control group, the application of banana leaf extract considerably boosted the growth characteristics of the plants, including shoot length, root length, and biomass. The use of banana peel extract as a natural fertilizer for plant development was the subject of a study by Das et al. (2018) concerning banana peels as a source of nutrient supplement for sustainable plant growth. The findings suggested that banana peel extract might serve as a sustainable substitute for chemical fertilizers by enhancing plant growth and nutrient absorption. Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija Another findings by Olanrewaju et al. (2019) examined the effect of banana leaf extract on the growth of maize plants. The study found that the application of banana leaf extract significantly increased the plant height, leaf area, and biomass compared to the control group. Based on the investigation conducted by Islam et al. (2017), the researchers investigated the effect of banana leaf extract on the growth and yield of tomato plants. The results showed that the application of banana leaf extract significantly increased the growth parameters of the plants, including plant height, leaf area, and yield, compared to the control group. Pechay (Brassica rapa) Pechay, sometimes referred to as bok choy or Chinese cabbage, is a leafy vegetable that is popular across Asia and has recently spread around the world owing to its many health advantages. The high nutritional content of pechay is one of its best-known health advantages. Pechay is an excellent source of the vitamins C and K, as well as folate, calcium, and iron, according to a research by Bora et al. (2021). These vitamins and minerals are essential for excellent health maintenance and the prevention of chronic illnesses like osteoporosis and cardiovascular disease. Antioxidants found in pechay aid in defending the body against oxidative damage and inflammation. Pechay extract was able to lessen inflammation in animals with colitis, a kind of inflammatory bowel disease, according to a research by Lee et al. (2018). The high antioxidant content of pechay, according to the experts, is what causes this impact. Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija Pechay has been discovered to have anti-cancer properties in addition to its antiinflammatory ones. Pechay extract has been shown in a research by Choi et al. (2014) to be able to stop the development of human breast cancer cells in vitro. The researchers hypothesized that glucosinolates, a kind of chemical present in cruciferous vegetables like pechay, were responsible for this impact. Blood sugar levels have been proven to be benefited with pechay. Pechay extract was able to reduce blood glucose levels in diabetic rats, according to a research by Zaid et al. (2020). The researchers hypothesized that this effect was brought on by substances known as alpha-amylase inhibitors, which can prevent the digestive system from breaking down carbs. Pechay is a nutrient-rich vegetable with a host of health advantages, such as anti-inflammatory, anti-cancer, and blood sugar-lowering properties. These results imply that pechay could be a beneficial supplement to a balanced diet and might aid in the prevention of chronic illnesses. Related Studies Banana (Musa acuminata) Peels and Leaves A study by Deocaris et al. (2018) investigated the effect of banana leaf and peel extracts on the growth and yield of tomato plants. The application of banana leaf and peel extracts considerably boosted the height, leaf area, and quantity of flowers and fruits that the tomato plants produced when compared to the control group, as to what the researchers observed. Also, the extracts were discovered to contain considerable levels of nitrogen, Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija phosphorus, and potassium, which are necessary elements for plant development. The study concluded that extracts of banana leaves and peel have promise as natural fertilizers for growing vegetables. Fertilizers often contain macronutrients like nitrogen (N), phosphorus (P), and potassium (K), which are crucial for plant growth and development. A study conducted by (Iszhan et al., 2021), the study discusses how to effectively cultivate water spinach using various fruit peels as organic fertilizer (Ipomoea Aquatica). Mango, pineapple, and banana peels were obtained separately from neighborhood fruit vendors. Individual fruit peels were washed, dried, and ground into powder. Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES) and a CHNOS analyzer were used to estimate the NPK content and analyze the chemical makeup of fruit peel powder. The analysis of three different fruit peels revealed that banana peel powder had the greatest levels of K and N, with concentrations of 26557.86 mg/L and 1.61%, respectively. In addition, fruit peel powder's ability to absorb water and detect electrical conductivity were both investigated. Plants were cultivated in various fruit peel powder treatments (applying peels from mango, banana, and pineapple), as well as control treatments (no fertilizer application). To find out how the fruit peel powder treatment affected plant development, plant height and leaf count per plant were analyzed. The addition of 3 g of powdered pineapple peels improved all water spinach growth metrics, with a mean maximum plant height of 20.833.01cm and a record-high 9 leaves per plant. So, it can be said that 3 g of powdered pineapple peels can effectively increase the use of fertilizers in agricultural productivity. Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija According to Susilawati et al. (2018), as banana leaf extract has a high nutritional content and can help plants develop, it has been investigated for its possible use as an organic fertilizer. The researchers concluded that banana leaf extract improved the development and crop of tomato plants. The use of banana fertilizer encourages the growth of tall, sturdy tomato plants. Maintaining the health of the plants' leaves, which in turn may stimulate the production of larger fruit, may also help them fight off pests. Certain nutrients are rich in banana leaves as well. The abundance of vitamins and minerals found in leaves, such as calcium, phosphorus, and iron, can enhance soil quality and encourage the growth of strong plants. Based on a study by Roy et al. (2006), the researchers investigate at how several vegetable crops' seeds and seedlings respond to water-soluble extracts from various banana plant sections. The test crops included okra, scarlet amaranth, radishes, cucumbers, beans, and ribbed gourds. The test solutions utilized as treatments were distilled water alone as the control and 100% extracts from the rhizome, root, leaf sheath, and leaf lamina of the banana plant, diluted to 15%, 25%, and 50% with distilled water. The germination of all the test crops was significantly inhibited by the plant extracts when compared to the control. The test crop's seed germination and seedling growth were most strongly inhibited by the extract from the rhizome of the banana plant, which was one of the plant's several sections. The lettuce seedlings were found to be the most sensitive of the test plant species, which all behaved differently to the rhizome extract. Other test plants' germination and seedling development were inhibited as extract concentration increased, with 100% concentration having the most inhibitory impact. Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija Any produced product, whether organic or chemical, that is put to soil to supply one or more nutrients necessary for plant development is referred to as a fertilizer. Based on the study conducted by (Mary Rose Olid et al., 2022), the study seeks at how banana peels can aid plant growth and increase productivity. In the study, banana peel powder is used as a natural fertilizer. The average amount of banana peel gathered was between 3 and 5 kg. The banana peels had been neatly sorted and dried. Before being turned into banana peel powder with a mortar and pestle, it was sun-dried for 5 to 7 days. In the research, the researchers used two different forms of fertilizer: artificial fertilizer (urea) and fertilizer that is made from banana peels. Potted seeds with and without two different types of fertilizer were used to compare the plant's growth and color. The current research aids the plants normal and abundant growth. The study evaluated the efficiency of fertilizing with banana peels on the leaves of radish and onion. The height and color of the radish and onion leaf plants are good indicators of how well the banana peel fertilizer worked. It has been shown that using banana peels as fertilizer can promote organic plant growth. Compared to plants planted with chemical fertilizer or without fertilizer, plants nourished with banana peels grew taller. As a result, radish and onion leaf plants treated with banana peels appear better in terms of stem height. Pechay (Brassica rapa) According to the research study entitled Performance and Growth of Pechay Using Fermented Plant Juice (2020), the pechay plant grows more quickly at higher concentrations of fermented plant juice than at lower concentrations, such as 50 milliliters of fermented plant juice, which has a slower growth rate than 100 milliliters. While the Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija researcher noticed that the 50ml of fermented plant juice was excessive because the growth was poor compared to the 100ml, the 200ml of fermented plant juice was nearly identical to 150ml. Therefore, the researchers decided that using 100ml of fermented plant juice in pechay plants was appropriate. The setup's pechay growth was normal, and the only intervention was the addition of water. Based on the study led by El-Sherbeny (2012), the researchers investigated the response of Brassica rapa to organic fertilizers, the organic fertilizers had the ability to significantly increase the number of leaves. Due to the use of both chemical and organic fertilization, the fresh and dry weights of leaves on turnip plants were significantly increased. By using different amounts of chemical or organic fertilizers, the characteristics of turnip plants' roots were significantly improved. The highest chemical fertilizer concentration (N200P200 K100), where the increment values over the control reached to 109.6%, 355.7%, and 106.8%, respectively, was responsible for the maximum mean values of root length (cm), fresh and dry weight. The second level of NPK or humic acid, based on the previous finding, was sufficient to achieve the highest growth parameters or biomass, as represented by leaves and roots, but it can be advised humic acid as an organic fertilizer for producing organic products. Additionally, compost tea's ability to enhance the hydro-physical characteristics of the soil as well as its higher nutritional value contributed to its promotion effect on the growth characteristics of turnip plants. The study conducted by Andal et. Al entitled The Response of Brassica rapa (Pechay) to the Three Different Kind of Organic Fertilizer; Vegetables, Rice and Bone that determine the Effects of various organic fertilizer types on Brassica rapa (Pechay), which Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija will produce the best height and most leaves. The goal of the study was to determine how pechay plants responded to the three different types of organic fertilizer and to compare the results with a controlled environment. The findings indicate that the vegetable waste fertilizer has the most leaves and the highest mean height. Moreover, the organic fertilizer that produce the most leaves and higher mean height. Synthesis Usually referred to as bok choy, pechay is a vegetable plant with small, green leaves that are leafy and have a white stalk. Pechay grows in tropical country. The study conducted by Andal et. Al determines the effects of various organic fertilizer types on Brassica rapa (Pechay), the study shows that the vegetable waste produce the highest height and most leaves among the bone and rise fertilizer. Similarly, the study led by El-Sherbeny (2012), the researchers investigated the response of Brassica rapa to organic fertilizers and reveals that organic fertilizers had the ability to significantly increase the number of leaves of Petchay. Fertilizers often contain macronutrients like nitrogen (N), phosphorus (P), and potassium (K), which are crucial for plant growth and development. A study by Deocaris et al. (2018) investigated the effect of banana leaf and peel extracts on the growth and yield of tomato plants. The application of banana leaf and peel extracts considerably boosted the height, leaf area, and quantity of flowers and fruits that the tomato plants produced when compared to the control group, as to what the researchers observed. Additionally, Banana peel has been found to contain a variety of vitamins and minerals such as phosphorus, iron, calcium, magnesium, and sodium (Hikal et. Al, 2022) and Banana leaves consist of Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija potassium, calcium, phosphorus, and magnesium (Barode, 2022). Thus, According to Susilawati et al. (2018), as banana leaf extract has a high nutritional content and can help plants develop, it has been investigated for its possible use as an organic fertilizer. Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija CHAPTER III METHODOLOGY This chapter showcases all the procedures that will be use in conducting this Project Research such as the research design, collection of materials, experimentation, data analysis, and product manufacturing. Research Design This study will use experimental research design method– a systematic and scientific approach to research in which the researchers manipulate one or more variables, and controls and measures any change in other variables. Research Locale This study will be conducted at Barangay District II, Mendoza residence, one of the researcher’s house. The area is stress free and has an annual mean temperature of 24°C. Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija Dist 2 Collection and Preparation of Musa acuminata Peels The banana peels will be obtained at Dr. Ramon De Santos National High School Canteens. Banana peels will be boiled for 3-5 minutes to acquire its extract. After cooling, banana peel extract will be kept in a bottle and will be store in cool dry area for further use. Collection and Preparation of Musa acuminata Leaves The banana leaves will be obtained at Barangay District II, one of the researcher’s house. Banana leaves will be boiled for 3-5 minutes to acquire its extract. After cooling, the banana leaves extract will be kept in a bottle and will be store in cool dry area for further use. Collection and Preparation of Brassica rapa (Pechay) Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija The pechay will be purchased at the Public Market of Cuyapo, Nueva Ecija. After obtaining the pechay, it will be placed in a seedling bag and placed in a cool environment for better growth. Procedure Experimental Set-Up Half kilogram of soil was placed in each plant. Nine (9) medium-sized plastic pots were prepared. There were three (3) pots for each treatment, for the Musa acuminata peels and leaves extract, with commercialized chemical fertilizer and without any kind of fertilizer. Ten (10) ml of Musa acuminata peels and leaves extract was poured in Treatment 1 every day. Ten (10) ml of commercialized fertilizer was poured once every three (3) days. Growth responses were measured by the plant’s height, number of leaves, color of leaves and weight of the Petchay. Data to be Gathered 1. The difference of the growth of pechay in terms of height, number of leaves, weight, and color of leaves, compared to the control supplemented with the organic fertilizer 2. The difference of the growth of the pechay in terms of height, number of leaves, weight, and color of leaves compared to the growth of pechay supplemented with commercial fertilizer. Statistical Analysis Dr. Ramon De Santos National High School San Antonio, Cuyapo, Nueva Ecija The statistical tool that used in the study “The Effect of Banana (Musa acuminata) Peels and Leaves Extracts in the Growth of Pechay (Brassica rapa)” is the One-Way ANOVA or "Analysis of variance". Data will be analyzed by their weekly growth average and final weight after 30-35 days.