7 Science Quarter 2 - Module 1: Parts of the Microscope and Their Functions AIRs - LM Science Quarter 2 - Module 1: Parts of the Microscope and Their Functions First Edition, 2020 Copyright © 2020 La Union Schools Division Region I All rights reserved. No part of this module may be reproduced in any form without written permission from the copyright owners. Development Team of the Module Author: Edith A. Dela Cruz Editor: SDO La Union, Learning Resource Quality Assurance Team Illustrator: Ernesto F. Ramos, Jr., P II Management Team: ATTY. Donato D. Balderas, Jr. Schools Division Superintendent Vivian Luz S. Pagatpatan, Ph.D Assistant Schools Division Superintendent German E. Flora, Ph.D, CID Chief Virgilio C. Boado, Ph.D, EPS in Charge of LRMS Rominel S. Sobremonte, Ed.D, EPS in Charge of Science Michael Jason D. Morales, PDO II Claire P. Toluyen, Librarian II Science Quarter 2 - Module 1: Parts of the Microscope and Their Functions Target Have you ever wondered how scientists were able to observe specimens which are not visible to the naked eye? Like the covid-19 virus, cells, bacteria? In the previous quarter you’ve learned that science is an accurate and realiable body of knowledge gathered through observation and extensive experimentations that passed through a process which we call scientific method. To do this scientists need tools that could help them observe properly and accurately, one of the important tools used by them is the microscope. In this module we’ll help you to be familiar with the parts of the microscope and their functions. After going through this module, you are expected to attain the following objectives: Most Essential Learning Competency • identify parts of the microscope and their functions (S7LT-IIa-1) Subtasks • define what is a microscope; • locate its parts through an illustration; and • give the function of each part. Pre-Test Multiple Choice. Read the following questions and write the letter of your answer on a separate sheet of paper. Label your paper Quarter 2 Module 1 Pre-test. ______1. Which of the following scientists made the first simple microscope? A. Aristotle C. Hans Jansen B. Antony Van Leewenhoek D. Zacharias Jansen ______2. Which of the following is a tool in science used to observe objects/specimen which are not visible to the naked eye? A. camera B. magnifying lens C. microscope D.telescope ______3. Which of the following parts of the microscope is used to gather light? A. eyepiece B. fine adjustment knob C. mirror D. stage ______4. Which of the following parts of the microscope holds the objective lenses? A. arm B. eyepiece C. revolving nosepiece D. stage clip ______5. Which of the following parts of the microscope supports the whole microscope? A. arm B. base C. condenser D. objective lens ______6. Which of the following parts of the microscope is used to tilt the microscope? A. eyepiece B. body tube C. draw tube D. inclination joint ______7. Which of the following parts of the microscope can you place the specimen to be observed? A. arm B. base C. stage D. stage clip ______8. Which of the following parts of the microscope holds the slide in place? A. arm B. base C. stage D. stage clip ______9. Which of the following parts of the microscope holds the eyepiece? A.base B. body tube C. diaphragm D. mirror _____10. Which of the following parts of the microscope regulates the light the microscope uses? A.base B. body tube C. diaphragm D. mirror _____11. Which of the following parts of the microscope is a part of its illuminating system? A. eyepiece B. inclination joint C. mirror D. stage _____12. Which of the following parts of the microscope is where you should look into to oberve the specimen? A. eyepiece B. inclination joint C. mirror D. stage _____13. Which of the following parts of the microscope are used to carry the microscope? A. arm and base C. eyepiece and diaphragm B. diaphragm and mirror D. stage and stage clip _____14. When focusing using the Low Power Objective which of the following should you use to focus the specimen? A. course adjustment knob C. fine adjustment knob B. diapraghm D. mirror _____15. When focusing using the High Power Objective which of the following should you use to focus the specimen? A. course adjustment knob C. fine adjustment knob B. diapraghm D. mirror Lesson 1 Parts of the Microscope and Their Functions Imagine a world where the concept of microorganisms are unknown. Would we be able to identify the cause of todays pandemic? COVID-19? Will we see the importance of washing our hands? What would our justification be regarding the pandemic we are experiencing? What would our healthcare system look like? Do we have the capacity to develop vaccines and medicines? Organisms which our naked eye can’t see greatly affects our lives, observing them is the key for us to know if they are harmful or beneficial, but how do we observe them with just our eyes? We need an instrument, a tool that could help us, and that tool is the microscope. Before you learn how this important science equipment works, let us first familiarize ourselves with its parts and their functions. Jumpstart Activity 1: Find Me! Encircle the parts of the microscope found in the Word Box. Use the attached Learner’s Copy in answering this activity. Word Box 1. eyepiece 2. course adjustment 3. fine adjustment 4. objectives 5. arm 6. stage clip 7. stage 8. inclination joint 9. body tube 10. revolving nosepiece 11. diaphragm 12. mirror 13. base Discover Three Dutch spectacle makers—Hans Jansen, his son Zacharias Jansen, and Hans Lippershey—have received credit for inventing the compound microscope about 1590, but it was the Dutch Antony Van Leeuwenhoek in the midseventeenth century who first made a simple microscope and used it to make discoveries. The microscope is a tool which can help you see tiny objects and living organisms. It makes them look bigger. The ability of the microscope to make the specimen bigger is called magnifying power or magnification. The microscope also has the capacity to distinguish small gaps between two separate points which humans cannot distinguish. It is called resolving power or resolution The light microscope uses diffused light from the sun or artificial light to illuminate the object to be observed. From its source, visible light passes through the small thin specimen to be observed through the glass lenses. As light passes through the lenses, it is bent so specimen appears bigger when it is projected to the eye. The form and structure of the specimen can then be seen because some of their parts reflect light. In this section you will find out the parts of the microscope and their functions. Parts of a microscope Figure 1. Parts of a light microscope The parts of a simple microscope are divided into three groups – • Mechanical parts • Magnifying parts • Illuminating parts Mechanical Parts of Microscope – The mechanical parts consists of the following components – ⇒ Body Tube – body tube separates the objective and the eyepiece and assures continuous alignment of the optics ⇒ Coarse adjustment – it is controlled by a pair of large knobs one on each side of the body. On rotating this knob, the tube moves with lenses. This is used with low power objective ⇒ Fine adjustment – The fine adjustment is used with high power (40X or 45X) and oil immersion (100X) objectives. This knob is present just below the coarse adjustment on either side of the body – gives clarity in observations. ⇒ Stage – The stage is a part of microscope where specimens to be observed is kept. This part is under the focus. It has a pair of clips, capable of moving the object horizontally as well as vertically. ⇒ Arm – The arm connects the body and the stage. It is used to hold the microscope while putting from one place to another. ⇒ Base– It is horseshoe shaped. It is the part that supports the whole microscope ⇒ Inclination joint- It is used to tilt the microscope. Magnifying Parts of a Microscope – the magnifying parts consist of objectives and eyepiece ⇒ Objective lens – • The lens that is present near to the object or specimen is called as the objective lens. It collects the light rays from the specimen and forms an inverted and magnified real image of the object. They are divided into 3 groups as follows – i. Low power objectives (4X, 10X) – magnifies the image 4 times or 10 times and provides maximum field of view. ii. High power objective (40X, 45X) – magnifies the image 40 or 45 times. iii. Oil immersion (100X) – magnifies the image 100 times and provides the minimum field of view. ⇒ Eyepiece – • It is present at the top of the microscope tube or near to the eye. It is where we look into to observe the specimen. Eyepiece uses a magnified real image produced by the objective to produce a more magnified virtual image. • The commonly used eyepiece has magnification 10X. it is also available in 5X, 15X and 20X. • Some microscopes are available with only one eyepiece called monocular; and other is binocular, which has two eyepieces. • The total magnification of the image of the object is the product of the magnifying power of eyepiece and that of the objective. For e.g. – when 10X eyepiece and 40X objective is used the magnification produced is – 10×40 = 400X & so on. Illuminating Parts of a Microscope – the illumination system provide proper illumination to the object. It comprises following parts – ⇒ Mirror – in case of external light source, the mirror is required to reflect the light beam from the source to the iris and condenser. It usually has two mirrors, mounted back to back. One side of the mirror is concave and the other is flat. The concave side is used without the condenser and the flat side is used the condenser. ⇒ Iris diaphragm/diaphragm – it is located below the stage between the mirror and the condenser. It regulates the amount of light. According to the necessity, the diaphragm can be closed for less light or opened for more light. ⇒ Condenser – the condenser is located at immediately under the stage. It is made up of a system of convex lenses, which focus light from the illuminating source on the plane of the object. The height of the condenser can be adjusted by rotating a knob, present on one side below the stage. Now that you know the parts of the microscope and their functions answer the following guide questions. GUIDE QUESTIONS 1. What is the function of the microscope? 2. What are the three main divisions of a microscope? 3. What are the parts of the microscope? Explore To intensify your understanding of the topic, do the activities below. Activity 1: Where Do I Belong? Complete the table below by listing the mechanical, magnifying and ixlluminating parts of the microscope. Write your answers on a separate sheet of paper. MECHANICAL MAGNIFYING ILLUMINATING Activity 2: Color by Number Color the parts of the microscope according to its given number. Label also its parts and give a brief explanation of their functions. Use the attached Learner’s Copy in answering this activity. # Color 1 Red 2 Orange 3 Yellow 4 Green 5 Blue 6 Pink 7 Violet 8 Black 9 Brown 10 Yellow green Yellow orange Red orange White 11 12 13 Function 1._______ 2.________ 3. ___________________ 4.__________________ 5.________________ 6.__________ 7.____ 9. _______ 8.________ 11.______ 10._______ 12.____________ 13.______ Deepen Based from what you have learned in this module, explain the importance of the microscope in the field of science or in the medical field using acrostic poem using the word microscope or any part of the microscope. Example: M-microscopes are important I-in our lives C-cause it is used to see things our eyes cant see R-really! Don’t you see? O-or just don’t realize? that S-science cannot progress without it, even the medical field cant C-corona virus is every where, pandemic is here, but the problem is we cannot see it, hear it, smell it, taste it, but with the use of powerful microscopes O-observing the virus and learning how to destroy it and make vaccines for it is P-possible, so E-everyone, I can therefore say, Microscopes are important Rubrics: 1 point 2 points 3 points Structure and Format The poem does not have a clear topic. More than 2 lines do not start with a letter in the topic word. More than 2 lines do not describe or relate to the topic of the poem. The poem has a topic, but 1-2 line(s) of the poem does/do not start with a letter in the topic word. 1-2 lines of the poem does/do not describe or relate to the topic of the poem. The poem has a clear topic. Each line of the poem begins with a letter in the topic word. Each line describes or relates to the topic of the poem. Creativity The poem does not contain any interesting word choice, vivid language, or poetic elements or techniques. The poem contains a couple of interesting word choices, uses some vivid language, and makes use of only one poetic element or technique. The poem contains more than two interesting word choices, uses vivid/descriptive language throughout the entire poem, and makes use of more than 1 poetic element or technique. Mechanics The poem contains 3 or more spelling, grammatical, or punctuation errors. The poem contains 1-2 spelling, grammatical, or punctuation errors. The poem does not contain any spelling, grammatical, or punctuation errors. Legibility Writing is not legible and the paper is messy. Writing is not legible in some places. Writing is legible and the paper is neat. Gauge Multiple Choice. Read the following questions and write the letter of your answer on a separate sheet of paper. Label your paper Quarter 2 Module 1 Gauge. ______1. Which of the following is the ability of the microscope to make the specimen bigger? A. magnifying power B. mechanical C. objective lens D. resolution ______2. Which of the following is the ability of the microscope to distinguish small gaps between two separate points which humans cannot distinguish? A. magnifying power B. mechanical C. objective lens D. resolution ______3. Which of the following parts of the microscope focus light from the illuminating source on the plane of the object? A. condenser B. fine adjustment knob C. mirror D. stage ______4. Which of the following parts of the microscope regulates the amount of light the microscope uses? A. arm B. eyepiece C. diaphragm D. stage clip ______5. Which of the following parts of the microscope supports the whole microscope? A. arm B. base C. condenser D. objective lens ______6. Which of the following parts of the microscope is used to tilt the microscope? A. eyepiece B. body tube C. draw tube D. inclination joint ______7. Which of the following parts of the microscope produces a magnified real image the specimen to be observed? A. arm B. eyepiece C. objective lens D. stage clip ______8. Which of the following parts of the microscope holds the slide in place? A. arm B. base C. stage D. stage clip ______9. Which of the following parts of the microscope holds the eyepiece? A.base B. body tube C. diaphragm D. mirror _____10. Which of the following parts of the microscope gathers light? A.base B. body tube C. diaphragm D. mirror _____11. Which of the following parts of the microscope is a part of its magnifying system? A. eyepiece B. inclination joint C. mirror D. stage _____12. Which of the following parts of the microscope produces a more magnified virtual image of the specimen? A. eyepiece B. inclination joint C. mirror D. stage _____13. Which of the following parts of the microscope are used to carry the microscope? A. arm and base C. eyepiece and diaphragm B. diaphragm and mirror D. stage and stage clip _____14. When focusing using the Low Power Objective which of the following should you use to focus the specimen? A. course adjustment knob C. fine adjustment knob B. diapraghm D. mirror _____15. When focusing using the High Power Objective which of the following should you use to focus the specimen? A. course adjustment knob C. fine adjustment knob B. diapraghm D. mirror Learner’s Copy (Detach from the module) NAME:_____________________________________ GRADE AND SECTION:______________________________ QUARTER 2 MODULE 1 Activity1: Find Me! Encircle the parts of the microscope found in the Word Box I C J V C T S K F F C M R C F R N E O M H A U O L L O M M O I E C R J I O T M O G O O A M A N V L E K R P A M K T W K N E R E O I B B R E V E I E E Y G T S A L N A U T T Y O R R S S T R A E G C E R C S L D I S P E A D J V I T Y E S Z O R O I L S A R D U N I D R M A I G E P I H P I J S G O J O B D T I L S U T F A F G Y E Y P S O O H R N G U S T M N O Word Box 1. eyepiece 2. course adjustment 3. fine adjustment 4. objectives 5. arm 6. stage clip 7. stage 8. inclination joint 9. body tube 10. revolving nosepiece 11. diaphragm 12. mirror 13. base O I N B A S J R F E M C C H I T H R I N I V A B E M E S I E K Y S A G M D A Y T M E E N T S E P T E O H M O P R U S O P J N H I Q F H O M B N O K O Y A K T L E T G O A Y I H O P E P A G U S C A A G H J N F K H D N O O D L E Learner’s copy (Detach from the module) NAME:_____________________________________ GRADE AND SECTION:______________________________ QUARTER 2 MODULE 1 Activity 3: Color by Number Color the parts of the microscope according to its given number. Label also its parts and give a brief explanation of their functions. 1_____________ 2.__________ 3.___________________________ 4._________________________ 5.________________________ 6.___________ 7._____ 8.___________ 9.______ 10.__________ 11._______ _ 12._________________ 13.______ # Color 1 Red 2 Orange 3 Yellow 4 Green 5 Blue 6 Pink 7 Violet 8 Black 9 Brown 10 Yellow green 11 Yellow orange 12 Red orange 13 White Function Activity 2: Where Do I Belong? MECHANICAL MAGNIFYING Body tube Objective lens Coarse Eyepiece Adjustment Fine Adjustment Arm Base Inclination joint ILLUMINATING Mirror Diaphragm Condenser GUIDE QUESTIONS 1. What is the function of the microscope? -used to observe objects/specimen our naked eyes cant see 2. What are the three main divisions of a microscope? -Mechanical, magnifying and illuminating 3. What are the parts of the microscope? -eyepiece, bodytube, coarse adjustment knob, fine adjustment knob, stage, stage clip, diaphragm, condenser, revolving nosepiece, objectives/objective lens, arm, base, inclination joint, mirror Activity 1: Find Me! Pretest 1. B 6. D 2. C 7. C 3. C 8. D 4. C 9. B 5. B 10. C 11. 12. 13. 14. 15. C A A A C Answer Key 11 Gauge 1. A 6. D 2. D 7. C 3. A 8. D 4. C 9. D 5. B 10. D 11. 12. 13. 14. 15. A A A A C Activity 3: Color by Number References Science 7 Learner’s Material, First Edition 2017 Alvie J. Asuncion et.al https://www.britannica.com/technology/microscope/History-of-opticalmicroscopes https://microbenotes.com/light-microscope/ https://paramedicsworld.com/general-laboratory-information/light-opticalcompound-microscope/medical-paramedical-studynotes https://www.rcampus.com/rubricshowc.cfm?code=N2AC64&sp=yes& 12