Recombinant DNA Tools • • • • • • • • Synthesis of recombinant DNA Separate and visualize DNA Gene cloning in plasmids Gene cloning with PCR Genetic profile Synthesis of complementary DNA (cDNA) Gene editing with CRISPR-Cas9 system DNA sequence analysis Synthesis of Recombinant DNA Separate and Visualize DNA: Gel Electrophoresis Gene Cloning in Bacterial Plasmids Gene cloning: Polymerase Chain Reaction (PCR) Genetic Profile Synthesis of Complementary DNA (cDNA) CRISPR-Cas9 system Sequence Analysis Applications of Genetic Engineering 1. Gene Expression Analysis • • 2. RT-PCR Analysis of Expression of Single Genes Microarray Analysis Medical • • Diagnosis of diseases o Treatment of diseases o • Human gene therapy Forensic evidence o o 3. Restriction Fragment Length Polymorphism (RFLP) Short Tandem Repeats (STR) Restriction Fragment Length Polymorphism (RFLP) Genetically Modified Organisms • • • Agriculture Environmental clean-up Pharmaceutical Products Gene Expression Analysis RT PCR Analysis of Expression of Single Genes Gene Expression Analysis Microarray Analysis Diagnosis of Diseases Restriction Fragment Length Polymorphism (RFLP) Human Gene Therapy Forensic Evidence Short Tandem Repeats (STRs) Restriction Fragment Length Polymorphism (RFLP) Genetically Modified Organisms Animals Bacteria Genetically Modified Organisms Plants