COMMENTS IN THE DEVELOPMENT OF THE MS THESIS PROPOSAL OF EZEKIEL SALINAS Comments during the previous proposal defense held on 17 July 2023 Item No. Summarized Comments Comments made by Actions Undertaken 1 The proposal seems vague. What do you want to assess? Do note that a watershed is a relatively larger patch of ecosystem. The scope of your proposal is too broad. Please narrow down your title and objectives trickling down to an original and doable study. Sir Deign The topic was narrowed down to the distribution/diver sity of macroinvertebrat es along the Bued River. Referring to the comment of Deign, have you read some related studies that will support your proposal of using selected parameters (of a watershed) to frame refugia/refuge? Also, please reconsider your selection of sampling stations. Looking at the 19 stations would too much (re: geographical interspersions) Sir Rom 2 Other underlying parameters (external, abiotic) shall support the macroinvertebrat e study. Already removed the term refugia as it is deemed too broad. The use of refugia can be barely supported by the limited studies on macroinvertebrat es of montane stream environment. There shall only be 5 sampling stations to be considered, with site accessibility and considerable substrate as Remarks from the Adviser / the Reader criteria of selection. 3 Why did you use the term refugia? What are you trying to imply with it on watersheds? Establishing refugia varies per taxonomic group (example: caves are refuge/refugia of selected native bats) Please be specific. Sir Don/ Ma’am Liezel You may reconsider if the inclusion of socioeconomic survey is still necessary. The study will only account the river system water quality using macroinvertebrat e assemblages as a primary parameter (complementary with the established secondary datasets from public sources). The study shall be conducted not to establish areas of ecosystem refugia but to be able to determine refuges (functional ecosystem that can accommodate the subsistence of macroinvertebrat es). Omitted the inclusion of ecosystem valuation in the assessment of Bued Ecosystem. 4 Are you proposing a study which is already being implemented by the DENR? Do you intend to Ma’am Rose Clarified the scope and delimitations of the study. adopt only secondary datasets? 5 There shall be a closed discussion by the Thesis Committee regarding the approval of his proposal. It is up to Ma’am Zen and Sir Aris. Primary observation and data collection will only be applied for macroinvertebrat es assemblage’ whereas, abiotic factors (other environmental variables) derived from secondary sources will only support the inferences (on macroinvertebrat es of Bued River). Ma’am Meijei Major revision of the proposal: After the proposal (in the period of consultations for the revision of the proposal) The topic was changed by the proponent to “Understanding the Longitudinal Distribution Patterns of Macroinvertebrates along the WQMA- Bued River System” 6 The intention of selecting a study site has to be well discussed in the Introduction. Also, avoid pre-emptive statements especially if not accompanied by proper citations. Please revise by removing WQMA in the topic/title. The proposal must be properly packaged in such a way that the essence of research novelty is conspicuously stated. Please ensure that you are Ma’am Zen The introduction has been revised to discuss the macroinvertebrat es along Bued River as the main subject of the study; WQMA is clarified in the (intro) discussion as an existing clearcut mechanism of conserving/reha proposing a study that is not a repetition of mandated functions of the government. Change the title to “MACROINVERTEBRATE ASSEMBLAGES AS INDICATOR OF WATER QUALITY ALONG SELECTED SITES IN BUED RIVER, BENGUET, PHILIPPINES”. Consider reading the study of Azis & Abas (2021) bilitating the Bued River. The intention of proposing the study is to support the effective implementation of WQMA. If ever approved and successfully implemented, the study on macroinvertebrat e assemblage along Bued River will provide essential baseline on up-to-date status of the Bued River Ecosystem vis-a-vis (or in relationship) to its prevailing ambient environmental conditions. The study of Azis & Abas (2021) has been considered in the conceptual framework of the proposed study. Azis & Abas (2021) solely assessed an entire river ecosystem/a protected watershed area in Malaysia by deriving macroinvertebrat es distribution and diversity. External abiotic and environmental factors were only used as determinants or supporting parameters to understand ecological status. 7 Again, the research matrix Sir Aris has to be properly filled out so to be able to present a clear direction of your proposal. You had presented a very broad scope of thesis. I would like to remind you that hypothesis testing has to be doable and distinct. The research matrix has been revised to state the central ecological concept to be employed in the study. The classic River Continuum Concept (Vannote et al., 1980) will be considered in the study of diversity and distribution of macroinvertebrat es along the Bued River. Standard abundance/ diversity metrics shall be carefully studied using the framework of partitioned biological aggregation/distr ibution along a river continuum. Further, discussion on river continuity and patch dynamics shall be included to characterize spatial/ecologica l heterogeneity.