EGG OSMOMETER EXPERIMENT Name: Syarifah Alyah Maisarah Binti Syed Mohd Hatta Class: 4 Budi Date And Time: 2/6/2023 9.33 PM CONTENT 1. Credits 2. Introduction & Objective 3. List Of Apparatus And Material 4. Procedure 5. Conclusion 6. Discussion 7. Results 8. Cost Estimation INTRODUCTION AND OBJECTIVE Introduction: Egg membrane is semipermeable, therefore it can be a subtitute for visking tube. Objective: Understanding the movement of substances across plasma membrane CREDIT First, I would like to thank the principal of Sekolah Sains Sultan Haji Ahmad Shah Pekan, Puan Narizan binti Mamat for allowing me to conduct experiments related to the egg osmometer. Furthermore, I would also like to thank Puan Nur Aziemah for helping and guiding me in this experiment. Lastly, I appreciate all the support from my friends and family throughout the egg osmometer experiment. List Of Apparatus And Material Apparatus: 1. Glass jar 2. Hot glue gun Materials: 1. Fresh egg 2. Drinking straw 3. RO water (sea master) PROCEDURE 1. Crack the eggshell at one end with a blunt object without damaging the underlying shell membrane. Remove the shell fragment without tearing the membrane 2. At the other end of the egg, remove a small portion of the shell and puncture a tiny hole in the membrane. 3. Insert a plastic drinking straw into the hole. Seal it using a hot glue gun to make sure the seal does not leak. 4. Set the exposed membrane of the egg in a glass jar of RO water. Add water from time to time as needed to ensure that the water covers the membrane all the time. 5. Check the osmometer every hour over the next 12 hours. Each time you check it, record the time and measure the height of the fluid in the drinking straw. 6. Draw a graph to represent the data you have recorded. Conclusion Water molecules permeate from an area of high water potential to an area of lower water potential. DISCUSSION 1. Based on the graph, describe the relationship between the egg solution level and time The final level of the egg solution increases when the time increases. This is due to the diffusion of water across the selectively permeable membrane ( egg membrane) into the egg solution inside the egg. The diffusion of water is osmosis. 2. What causes the change in the level of the solution in the straw? The level of egg solution increase because of the movement of water molecules from inside the glass jar into the egg which is down the concentration gradient. 3. What inferences can you make about the size of water molecules and the pores of the egg membrane? The size of water molecules is smaller than the size of pores of the egg membrane. DISCUSSION 4. Predict the result if mineral water is replaced with 30% NaCl salt solution? Explain. The level of egg solution in the straw decreases. This is because the egg solution diffuses out of the egg into the salt solution inside the glass jar down its concentration gradient. Result Cost Estimation 1. Fresh egg - RM 0.51 x 5 2. Glass jar - RM 1.70 3. Drinking straw - RM 2.30 (one pack)