RESPIRATION It is the process by which energy is generated from the breaking down of carbohydrates in living cells. Types of respiration Aerobic Anaerobic Aerobic respiration The release of relatively large amounts of energy from the breaking down of carbohydrates in the presence of oxygen Word & symbol equation of anaerobic respiration Glucose + oxygen ENZYMES carbon dioxide + water + energy C6 H12 O6 (aq) + 6O2 (g) ENZYMES 6CO2 (g) + 6H2O (l) + 2830KJ/mol Uses of energy released from respiration 1. It is used to maintain a constant body temperature 2. It is used during active transport, in the selective reabsorption of vital minerals 3. It is used in the synthesis of macromolecules from micromolecules e.g. synthesis of proteins from amino acids 4. It is used during mitotic cell division which results in the growth of an organism 5. It is used during transmission of nerve impulses Anaerobic respiration It is the release of fairly small amounts of energy from the breaking down of carbohydrates in the absence of oxygen. Word & symbol equations of anaerobic respiration (In plants) Glucose carbon dioxide + ethanol + energy ENZYMES C6 H12 O6 (aq) + CO2 (g) + C2 H5 OH (l) + 118KJ/mol ENZYMES (In animals) Glucose lactic acid + energy ENZYMES C6 H12 O6 (aq) + ENZYMES 2C3H6O3 (g) + --------------+ 118KJ/mol Difference between Aerobic & Anaerobic respiration Aerobic Respiration Occurs in the presence of oxygen Occurs in the mitochondrion Releases fairly large amounts of energy Releases CO2 and H2O as by-products Anaerobic Respiration Occurs in the absence of oxygen Occurs in the cytoplasm matrix Releases fairly small amounts energy Releases ethanol & lactic acid as a by product Fermentation It is the release of fairly small amounts of energy from breaking down of glucose by extra -cellular enzymes of microorganisms in the absence of oxygen Both the word & symbol equation of fermentation are similar to that of anaerobic respiration in plants. Glucose carbon dioxide + ethanol + energy ENZYMES C6 H12 O6 (aq) + ENZYMES CO2 (g) + C2 H5 OH (l) + 118KJ/mol ACTIVITY; Experiment to show fermentation of flour by yeast Oil Glucose + Yeast suspension Lime water Procedure 1. Boil the water to expel the entire O2 and allow it cool 2. Use boiled water make a solution of 5% glucose & 10% suspension of yeast 3. Place 5cm of glucose solution in a test tube 4. Add 1cm of yeast suspension to the glucose in the test tube 5. Add a layer of paraffin / oil to exclude atmospheric O2 6. Connect the apparatus as shown above 7. Follow the same instructions to prepare a control experiment. This time use yeast suspension that has been boiled to kill yeast cells & denature enzymes 8. Wait for 15minutes before taking observations. The start of the experiment may be shown by bubbles escaping into lime water - In the case the experiment takes long to commence, warm the mixture in a warm water bath for 5minutes at 30 0C Results: The bubbles of the gas from the living yeast will turn lime water milky showing that CO2 is released. In the control set the lime water will not turn milky because the enzymes in the yeast cells were denatured by boiling therefore no fermentation took place How Lactic Acid is produce in muscles during exercise During exercise, the O2 supply may be insufficient to meet the energy demand. When this happens the cells produce energy by anaerobic respiration, lactic acid is produced as a by product. The accumulation of lactic acid causes muscles fatigue but is eventually reduced as oxygen intake returns to normal after the period of exercise. This shortfall of oxygen is called “Oxygen debt” and can be repaid by increased O2 intake. i.e. the person will continue to breathe fast & deep in order to draw more O2 into the lungs. This O2 will be used to oxidize lactic acid to carbon dioxide & water The Graph Showing Effect of Exercise on the Lactic Acid Concentration of Blood. Normal period of O2 debt normal Courtesy of Lebsy mb 71726846 activity repaid