Move System Reviewer: Phylum Protozoa/ Protista and Porifera Protozoa/Protista Protozoa are economically important parasites which live in or upon the bodies of other animals or plants. Every species has a definite form and the size of the individuals vary within definite, usually narrow, limits. → They possess a single nucleus, if is spherical. → Contractile vacuoles, food and stationary vacuoles containing fluid may be present in the bodies of protozoa. → Phylum protozoa are heterogeneous assemblage of some 50,000 cellular or single- cell organism found worldwide in most habitats. → Protozoa means ‘first animal’ the simplest form of animal life. → Protozoa are unicellular microorganisms (eukaryotes) that lack cell walls. → They are found in marine habitat or soil, in fresh water bodies, symbiotic, some forms are parasites in other organisms. → Protozoa depends on nutrition, temperature, pH, and some depends on sunlight. Locomotory Organ 1. Cilia ➢ tiny hair-like appendages ➢ The term ‘cilia’ is a Latin term meaning eyelash indicating the tiny eyelash-like appearance. 2. Pseudopod ➢ from the Greek words pseudes and podos, meaning “false” and “feet” respectively. ➢ These projections extend and contract. ➢ For locomotion and capture of prey. 3. Flagellum ➢ The word “flagellum” means “whip”. ➢ a whip-like appearance that helps to propel a cell through the liquid The different protozoans commonly seen in hay infusion: 1. Ciliophora ➢ Moves with cilia ➢ Paramecium 2. Mastigophora ➢ Moves with flagellum ➢ Euglena 3. Sarcodina ➢ Moves with pseudopods ➢ Amoeba 4. Chilomonas ➢ Has 2 flagella Parts of a protozoan Economic importance of Protozoans: Divided into two: 1. Beneficial Protozoa 2. Harmful Protozoa Beneficial Protozoa 1. Food ➢ Provide food for insect larvae, crustaceans and worms, which are taken by large animals like fishes, lobsters, clams, and crabs, which are eaten by man. Thus, they form sources of food supply to man both directly and indirectly. 2. Symbiotic Protozoa ➢ live in the gut of termite, which help in the digestion of cellulose. The digested cellulose is utilized by the host. ➢ 3. Insect Control ➢ control harmful insects by persisting their bodies. 4. Helpful Sanitation ➢ living in polluted water feed upon waste organic matters and thus purify it. Many protozoa feed upon bacteria and play important role in the sanitary betterment and keeping water safe for drinking. 5. Industry ➢ The skeletal deposits of marine protozoa (Foraminifera and Radiolaria) from oceanic ooze at the sea-bottom. About 30% of oceanic bed is covered with the Globigerina ooze. These skeletal deposits are put to many uses. Some are employed as filtering agents; others are made into chalk and still others are used for abrasives. 6. Building Material ➢ The skeletal deposits in due course of time change into the limestone rock. Limestone is provided by the Globigerina ooze, such as that of cliffs of Dover, which have played an important role in the defense of England. 7. Oil Exploration ➢ Petroleum is organic origin. The skeletal deposit of Foraminifera and Radiolaria are often found in association with oil deposits. 8. Scientific Study ➢ Many protozoa are used in biological and medical researches. A Holotricha. Tetrachymena geleii is used in nutritional research. The effects of various foods and poison have been investigated on this protozoan. Harmful Protozoa 1. Pollution of Water ➢ Drinking water in natural condition is made unpalatable by 2. 3. 4. 5. the reproduction of some freeliving protozoa in it Destruction of Animals of Food Value ➢ dinoflagellates like Noctiluca and Gonyaulax, when become abundant, are responsible for turning the ocean red. The water becomes foul and cause toxic reaction to molluscs like clam oysters and mussels and they become unfit for eating by human beings. Destruction of Wooden Articles ➢ Some flagellater like Trichonympha and Colonympha live in the gut of termites and help in the process of cellulose digestion. In the absence of these flagellates the termites will die or change their diet. Thus such protozoa indirectly help in distraction of wooden articles and books. Reduction in Fertility of Soil ➢ These protozoa feed on nitrogenfixing bacteria thus reduce the fertility of soil. Parasitic Protozoa ➢ Ectoparasites - when the parasite lives on the external surface of the host. ➢ EndoparasitesWhen the parasite lives inside the body of the host a. Coelozoic i. When the parasite lives in the alimentary canal or cavities of host body. ii. Histozok • Which occupie s space betwee n the iii. host cells. Cytozoic • These live inside the host’s cell. Porifera Porifera are also known as “sponges”. They are primitive, sessile, mostly marine, water dwelling filter feeders that pump water through their bodies to filter out particles of food matter. → With no true tissues (parazoa), they lack muscles, nerves and internal organs. → Their similarity to colonial choanoflagellates shows probable evolutionary jump from unicellular to multicellular organisms. → They are both asexual and sexual. Their skeleton is made up of collagen and spicules. → Porifera are unidirectional driven by flagella. They have cellular level organization. → Different cells perform different functions. Representative 1. Class Calcarea ➢ They are characterized by spicules made of calcium carbonate in the form of calcite or aragonite. While the spicules in most species have three points, in some species they have either two or four points. 2. Class Hexactnellida ➢ are sponges with a skeleton made of four- and/or six-pointed siliceous spicules, often referred to as glass sponges. 3. Class Demospongiae ➢ They are sponges with a soft body that covers a hard, often massive skeleton made of calcium carbonate, either aragonite or calcite. ➢ They are predominantly leuconoid in structure. ➢ Their "skeletons" are made of spicules consisting of fibers of the protein spongin, the mineral silica, or both. ➢ Where spicules of silica are present, they have a different shape from those in the otherwise similar glass sponges. Some parts of a Sponge: 1. Spongocoel → Large central cavity of Sponges. → Water enters the spongocoel through hundreds of tiny pores called OSTIA and exits through a larger opening called OSCULUM. → Depending on the body plan of the sponge, the spongocoel could be a simple interior space of the sponge or a complexly branched inner structure. → Regardless of body plan or class, the spongocoel is lined with choanocytes, which have flagella that push water through the spongocoel, creating a current. The spongocoel is lined by a variety of cell types, each having a unique function: a. Porococytes → These cells line the pores of the sponge. They are the structure through which water is taken into the organism. b. Choanocytes → These cells exhibit flagella that create inward currents of water for the sponge's stationary filterfeeding mechanism. c. Amoebocytes → These are motile cells that perform various digestive functions within the sponge by transporting/storing food and excreting waste. 2. Flagellated Chamber → one of the outpouchings of the wall of the central cavity of a sponge that is lined with choanocytes and connects with incurrent canals through prosopyles 3. Prosopyle → the aperture between incurrent and radial canals in some sponges 4. Apopyle → one of the openings by which the water passes out of a radial canal or flagellated chamber of a sponge 5. Ostium → a series of tiny pores all over the body of a sponge that let water into the sponge. → One of these is called an ostium. → water flows into a sponge through cells with pores (these cells are called porocytes) located all over its body. 6. Osculum → an excretory structure in the living sponge, a large opening to the outside through which the current of water exits after passing through the spongocoel. → Wastes diffuse into the water and the water is pumped through the osculum carrying away with it the sponge's wastes. 7. Cells Present i. Pinacocytes o are thin walled and flat cells. They line the outer surface of a sponge. o Pinacocytes are slightly contractile. o Their contraction can change the shape of some sponges. o Some pinacocytes forms tube like contractile porocytes. o Porocytes regulate water circulation. o The openings of the porocytes are pathways to water movement of water through the body wall. ii. Mesohyl o Mesohyl is a jelly like layer present below the pinacocytes. o Amoeboid cells are present in it. o These cells are called mesenchyma cells. o The meseuchyma cells freely move in the mesohyl. These cells are specialized for reproduction, secreting, skeletal elements, transporting and storing food and forming contractile rings around openings in the sponge wall. iii. Choanocytes o Choanocytes or collar cells are present below the mesohyl. o They form the lining of the inner chamber. o Choanocytes are flagellated cells. o They have a collar like ring of microvilli surrounding a flagellum. o Microfilaments connect the microvilli. o It forms a netlike structure within the collar. o The flagellum creates water currents through the sponge. o The collar filters microscopic lbod particles from the water. o Collar cells arc also present in a group of protists called choanollagellates. o Choanocytes are present in sponges and choantlagellaes. It suggests an evolutionary link between these groups. Canal System of Poriferans: → represents the transitional grade between the simplest ascon type and more complex ones. → The complication is due to the out-pushing of the wall into finger-like projections, called the radial canals, at regular intervals. → In this type of canal system, choanocytes are only limited to the radial canals. Their detailed account has already been described with the biology of Sycon. 1. Asconoid Type → is re-garded to be the most simple and primitive grade of canal system. → Asconoid type is present in these sponges whose body is vase-like and radially symmetrical. → The asconoid type of canal system is characterised by the presence of a complete continuous layer of choanocytes lining the spongocoel interrupted only by the porocyte. 2. Syconoid Type 3. Leuconoid Type → In this type of canal system, the choanocyte lining of radial canals evaginates into many small chambers which repeat the same process to give rise to a cluster of small flagellated chambers. → In many cases dermal pores open into subdermal spaces. → The subdermal space and incurrent canals lead into the small rounded flagellated chamber through an opening, termed as prosophyle. → The flagellated chambers open by apopyles into excurrent canals which form large tubes. → The largest one leads to osculum. Few gradations of leuconoid type of canal system are seen in sponges. 4. Rhagon Type → The body is conical with osculum situated at the summit. → The spongocoel is bordered by oval flagellated chambers, opening into it by wide apopyles. → Between the epidermis and the chambers lies a considerably thickened mesenchyme which is traversed by incurrent canals and subdermal spaces. → The rhagon type is a little complex than the complex syconoid type and is found in the larval stage of Demospongiae. Different type of Spicules: Spicules are microscopic crystalline structures which gives the sponges their rigidity and form. or colloidal silica is deposited. Accordingly, spicules are of two types: a. Calcareous spicules: → The organic material in this type of spicules is calcium carbonate or calcite. This is the characteristic of the sponges of class Calcarea. b. Siliceous spicules: → The organics material in this type of spicules is Colloidal silica or Silicon. These types of spicules are the characteristic of the sponges of class Hexactanellida. On the basis of size and function: Spicules can be of large size or small size. Accordingly, spicules can be of two types: a. Megascleres: → These are larger spicules constituting main skeleton of sponge body. b. Microscleres: → These are the small spicules occurring interstitially. On the basis of number of axes and rays: Spicules may occur in several forms like the simple rod form or in the form of forks, anchors, shovels, stars, plumes etc. The spicule forms depend on the presence of number of axes and rays. Accordingly, they can be divided into the following forms: • Spicule consists of spines or rays that radiate from a point. These are secreted by special mesenchymal amoebocytes called scleroblast cells. On basis of type of deposit on core organic matter: All kinds of spicules have a core of organic material around which either calcium carbonate Monaxon: These kinds of spicules are formed by the growth along one axis. They may be straight needle-like or rod like or may be curved. Their ends may be pointed or hooked or knobbed. Monaxons can be both calcareous and siliceous types. rays are characteristic of calcareous sponges. → If the elongated ray bears a disc at both ends, it is called as amphidisc. • Triaxon: These spicules have three axes that cross one another at right angles to produce six rays. Thus it is also called hexactinal spicule. These triaxon spicules are characteristic of glass sponges of the class Hexactanellida. • Polyaxon: These are the spicules with several equal rays radiating from a central point. They may be grouped to give star-like appearance. Polyaxon spicules are found along with microscleres. These monaxon spicules are further divided into two kinds: o o • Monactinal- the growth of spicule takes place only in direction Diactinal- The growth of spicule takes place in both directions. the one the the Tetraxon: These spicules have four rays each pointing in different direction. Usually one of the four rays is elongated giving the appearance of a crown of 3 rays. Such spicules are called as triaenes. → Sometimes all the rays are equal, when all the rays are equal it is termed as calthrops. → When all the four rays persist, it is called as tetraradiate or quadriradiate. → Sometimes one of the rays is lost and then it is known as triradiate. These triradiate