Self-assessment questions for Lecture 1 1) Compare storage of information in eukaryotic and prokaryotic cell, which is typical only for eukaryotic cell? [Correct answer - 1 point]: a) Plasmids and nucleus b) Nucleus, mitochondria, chloroplasts c) Nucleoid and plasmids d) Only nucleus 2) Nucleocapsid is a structure found in [Correct answer - 1 point]: a) Prokaryotes b) Viruses c) Plants d) Eukaryotic nucleus 3) In replication process new RNA is synthesized from DNA (choose true/false) [Correct answer - 1 point]. 4) Prokaryotic cell has “end replication problem" (choose true/false) [Correct answer - 1 point]. 5) The picture shows fragment of …………… (write abbreviation of nucleic acid – RNA or DNA). Write corresponding nitrogen bases in the figure? Indicate 5`and 3` ends. [Correct answer – 1.5 points]. 6) Choose all cell structures involved in storage of genetic information [Correct answer – 2 points]: Pili Cell membrane Flagellum Mitochondria Plasmids Nucleus Endoplasmic reticulum Nucleoid 7) Match the description (1-3) to the one of given explanations (A-F) [Correct answer – 2 points]: 1. Viruses A Small, circular RNA molecules without capsid, mostly infect plant cells 2. Viroids B Infect only bacteria 3. Prions C Contain RNA or DNA and plasmids 4. Bacterio- D Infectious agents, consist of nucleocapsid phages E Infectious protein particles without nucleic acid F Extracellular state of virus 8) Fill in the gaps with corresponding terms in scheme “Flow of information in living organism”: DNA, RNA, reverse transcription, replication, transcription, translation, from information in the gene till protein, RNA replication. Indicate in which direction transfer of information is not possible [Correct answer – 2 points]. 9) List 4 functional types of RNA molecule [Correct answer – 2 points]. 10) Explain, why differences in replication of leading and lagging strands exist? Shortly describe, how both strands are replicated [short, open type question, maximum 3 points].