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Self assesment questions Lecture1 answers

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Self-assessment questions for Lecture 1
1) Compare storage of information in eukaryotic and prokaryotic cell, which is typical only for
eukaryotic cell? [Correct answer - 1 point]:
a) Plasmids and nucleus
b) Nucleus, mitochondria, chloroplasts
c) Nucleoid and plasmids
d) Only nucleus
2) Nucleocapsid is a structure found in [Correct answer - 1 point]:
a) Prokaryotes
b) Viruses
c) Plants
d) Eukaryotic nucleus
3) In replication process new RNA is synthesized from DNA (choose true/false) [Correct answer
- 1 point]. False (Replication process generate new strand of DNA based on template (old)
DNA strand. Semiconservative replication model).
4) Prokaryotic cell has “end replication problem" (choose true/false) [Correct answer - 1 point].
False (prokaryotic cell has circular chromosome, no “end replication problem”).
5) The picture shows fragment of …DNA… (write abbreviation of nucleic acid – RNA or DNA).
Write corresponding nitrogen bases in the figure? Indicate 5`and 3` ends. [Correct answer –
1.5 points].
6) Choose all cell structures involved in storage of genetic information [Correct answer – 2
points]:
Pili
Cell membrane
Flagellum
Mitochondria
Plasmids
Nucleus
Endoplasmic reticulum
Nucleoid
7) Match the description (1-3) to the one of given explanations (A-F) [Correct answer – 2
points]:
1-D
1. Viruses
A Small, circular RNA molecules without capsid,
2-A
mostly infect plant cells
3-E
2. Viroids
B Infect only bacteria
4-B
3. Prions
C Contain RNA or DNA and plasmids
4. Bacterio- D Infectious agents, consist of nucleocapsid
phages
E Infectious protein particles without nucleic acid
F Extracellular state of virus
8) Fill in the gaps with corresponding terms in scheme “Flow of information in living organism”:
DNA, RNA, reverse transcription, replication, transcription, translation, from information in
the gene till protein, RNA replication. Indicate in which direction transfer of information is
not possible [Correct answer – 2 points].
9) List 4 functional types of RNA molecule [Correct answer – 2 points]. mRNA; tRNA;
rRNA; ncRNA.
10) Explain, why differences in replication of leading and lagging strands exist? Shortly
describe, how both strands are replicated [short, open type question, maximum 3 points].
Mention: antiparallel strands of DNA; 5`-3` direction of synthesis; replication fork
movement; Okazaki fragments; primase; DNA polymerase; free dNTPs; ligase; RNase.
Describe how all of mentioned terms ensure the process.
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