HOLY ANGEL UNIVERSITY ANGELES CITY COLLEGE OF NURSING COURSE SYLLABUS I. Course title : Microbiology And Parasitology II. Course description: This course deals with the study of the general characteristics, pathogenicity, and laboratory procedures for detection of bacteria, viruses, fungi, protozoa, and parasites III. Course credit: Placement: 2nd semester, level II Units: 5 units IV. Pre- requisite: Human Anatomy and Physiology, Primary Health Care I, Health Ethics V. Course general objectives (broad competencies): At the end of the semester, the students should be able to: 1 Define Microbiology and Parasitology, and identify their significance to the nursing profession. 2 Discuss the principles in Microbiology and Parasitology, including the structure, cellular metabolism, and the genetics of growth 3 Discuss how microbial and parasitic growth can be minimized or stopped using different techniques that are feasible for the working and living conditions of contemporary times 4 Trace the historical development of Microbiology and Parasitology VI. Course Content Meetings 1 st week Topic I. Introduction to Microbiology and Parasitology A. Definition 1. Microbiology 2. Parasitology Learning Competencies At the end of the unit, the student should be able to: 1. Define Microbiology, pathogen, nonpathogen, and opportunistic pathogen Outcomes/Stude nt Lecture - Discussion with use of transparencies and power point presentation Assessment Tools > Quizzes > Recitation > Assignements Resources > Microbiology for the Health Sciences, Burton p. 1-14 Meetings Topi c 1. Pathogenic microorganisms 2. Non pathogenic microorganisms 3. Opportunistic microorganisms C. Importance D. Microbiology Subprovinces 1. Bacteriology 2. Phycology 3. Protozoology 4. Mycology 5. Virology E. Pioneers in the science of microbiology Learning Competencies 3. Enumerate and differentiate the different types of parasites 4. List several reasons why microorganisms are important 5. Explain the relationship between microorganisms and infectious diseases 6. Enumerate and differentiate the several subprovinces of microbiology 7. Outline some of the contributions of Leeuwenhoek, Pasteur, and Koch to microbiology 8. Explain the germ theory of disease 9. Outline Koch's postulates and cite some circumstances in which they may not apply 2nd week II. Cytology A. Classification 1. Acellular 2. Cellular B. Procaryotic Cell Structure 1. Domain bacteria 2. Domain Archaea C. Eucaryotic Cell Structure 1. Algae 2. Fungi 3. Protozoa D. Comparing Procaryotic and Eucaryotic Cells E. Reproduction of organisms 3rd week III. Pathogenesis of Infectious Disease A. Terminologies 1. Infection and Infectious Disease Outcomes/Stude nt At the end of the unit, the student should be able to: 1. Compare and contrast the different classification of microbes 2. Cite a function of the different organelles/ parts of a procaryotic cell 3. Cite a function of the different organelles/ parts of a eucaryotic cell 4. Identify different forms of reproduction among microorganisms Assessment Tools Resources Lecture - Discussion with use of transparencies and power point presentation > Quizzes > Recitation > Assignements > Microbiology for the Health Sciences, Burton p.41-128 Lecture - Discussion with use of transparencies and power point presentation > Quizzes > Recitation > Assignements > Microbiology for the Health Sciences, Burton p.360-367 Meetings Topi c 3. Acute, Subacute, and Chronic Diseases 4. Signs and Symptoms of a Diseases 5. Clinical and Subclinical Diseases 6. Primary and Secondary Infections B. Periods/Phases in the course of an Infectious Disease 1. Incubation 2. Prodromal 3. Period of Illness 4. Convalescent C. Steps in Pathogenesis 1. Entry 2. Attachment 3. Multiplication 4. Invasion 5. Evasion of Host Defenses 6. Damage to Host D. Virulence and Virulence Factors 4th week Learning Competencies develop an infectious disease following exposure to a pathogen 3. Differentiate localized and systemic infections 4. Explain the differences among acute, subacute, and chronic diseases 5. Define and differentiate between signs and symptoms of a disease and give examples to each 6. Differentiate clinical and subclinical diseases 7. Differentiate primary and secondary diseases 8. List the periods in the course of an infectious disease 9. List the steps in pathogenesis in proper order 10. Define virulence and virulence factors 11. List bacterial structures that serve as virulence factors At the end of the unit, the student should be able to: IV. Microbial Ecology A. Symbiosis 1. Neutralism 2. Commensalism 3. Mutualism 4. Parasitism 5. Synergism B. Indigenous Microflora C. Epidemiology 1. Terminologies Outcomes/Stude nt 1. Define ecology and microbial ecology 2. List and define the categories of symbiotic relationships 3. Discuss the beneficial roles of the indigenous microflora of the human body 4. Define epidemiology 5. Define and differentiate among communicable, contagious, and noncommunicable disease Lecture - Discussion with use of transparencies and power point presentation Assessment Tools > Quizzes > Recitation > Assignements Resources > Microbiology for the Health Sciences, Burton Health Sciences p.249- 300) Meetings 5th - 6th week Topi c d. Zoonotic Disease e. Incidence f. Prevalence g. Mortality h. Sporadic Disease i. Endemic Disease j. Epidemic Disease k. Pandemic Disease D. Chain of Infection 1. Pathogen 2. Reservoir a. Living Reservoir b. Non-living Reservoir 3. Portal of exit 4. Mode of Transmission a. Contact Transmission b. Vehicular Transmission c. Vectors 5. Portal of Entry 6. Susceptible Host Learning Competencies Outcomes/Stude nt 7. Differentiate among sporadic, endemic, epidemic, and pandemic diseases 8. List the components of the chain of infection in proper order 9. identify three examples of living reservoirs and three examples of non-living reservoirs 10. List and differentiate the modes of infectious disease transmission V. Host Defense Mechanisms A. Non Specific Host Defense Mechanism At the end of the unit, the student 1. First Line of Defense should be able to: a. Skin b. Mucous Membrane 1. Define and differentiate non-specific host c. Respiratory System defense mechanism and specific host d. Digestive System defense mechanism e. Genito-urinary tract 2. Describe the three lines of defense used f. Indigenous Microflora by the body to combat pathogens 2. Second Line of Defense 3. Identify ways by which the skin is a. Transferrin protected from pathogens b. Fever 4. Identify ways by which the mucous c. Interferons membrane protects the body from d. Complement System pathogens Lecture - Discussion with use of transparencies and power point presentation Assessment Tools > Quizzes > Recitation > Assignements Resources > Microbiology for the Health Sciences, Burton p.380-436) > Essentials of Anatomy & Physiology, Seeley, p.392-419 Meetings Topi c f. Cytokines g. Inflammation h. Phagocytosis B. Specific Host Defense Mechanism 1. Comparing Antibody Mediated and Cell Mediated Immunity 2. Acquired Immunity a. Active Natural b. Active Artificial i. Vaccination ii. Types of Vaccines c. Passive Natural d. Passive Artificial 3. Antibody Mediated Immunity a. Antigens b. Antibodies 4. Cell mediated Immunity 5. Hypersensitivity Reaction a. Immediate Type i. Anaphylactic Reaction (Type I) ii. Cytotoxic Reaction (Type II) iii. Immune Complex Reaction (Type III) b. Delayed Type / Cell Mediated End of 6th 7th week VI. Mycology A. Definition B. Characteristics C. Structure D. Reproduction E. Types Learning Competencies Outcomes/Stude nt protected from pathogens 7. Identify ways by which the genitourinary tract is protected from pathogens 8. Explain how the second line of defense protects the body against pathogens 9. Enumerate the benefits of fever 10. List the cardinal signs and symptoms of inflammation 11. Discuss the primary purposes of the inflammatory response 12. Outline the steps of phagocytosis 13. Describe the mechanism how interferons protect the body against infectious disease 14. Differentiate antibody-mediated and cell-mediated immunity 15. Define an antigen and an antibody 16. List and differentiate among the five types of antibodies 17. Differentiate and distinguish among the four types of acquired immunity 18. Identify and differentiate the types of vaccines 19. Identify the importance of vaccines 20. Construct a diagram regarding antibody production 21. List the differentiate types of T-lymphocytes 22. Name and differentiate the four types of hypersensitivity reactions PRELIM EXAMINATION At the end of the unit, the student should be able to: 1. Define mycology 2. enumerate the characteristics of fungi 3. Draw and label the different parts of a typical Lecture - Discussion with use of transparencies and power point presentation Assessment Tools > Quizzes > Recitation > Assignements Resources > Microbiology for the Health Sciences, Burton p. 118-130; 447-514 Meetings Topi c 1. Yeasts 2. Molds 3. Fleshy Fungi F. Classification G. Mycoses/Fungal Infections 1. Superficial Mycosis 2. Systemic Mycosis 8th - 12th week VII. Virology A. Definition B. Nature and Characteristic C. Morphology and Size D. Naming of Viruses E. Virus Multiplication Cycle 1. Lytic cycle 2. Lysogenic cycle F. Pathogenicity and Cytopathic Effect G. Classification 1. Deoxyribovirus i. Poxvirus ii. Human Herpes virus iii. Adenovirus iv. Papovirus v. Hepadnavirus 2. Ribovirus i. Orthomyxovirus ii. Paramyxovirus iii. Picornavirus iv. Rhabdovirus v. Togavirus Learning Competencies Outcomes/Stude nt fungus 4. Enumerate and describe the different types of fungal reproduction 5. Differentiate and compare the different types of fungi 6. Enumerate the classification of fungi 7. Identify the specific fungus involved in a mycosis 8. Explain the pathogenicity, signs, and symptoms of a typical mycosis 9. identify nursing interventions in the prevention, treatment, and rehabilitation of patients with mycosis At the end of the unit, the student should be able to: 1. Differentiate a virus from a bacterium 2. Discuss the differences between viroids, virions, and the disease that they cause 3. Draw and label the different parts of a virus 4. Describe the chemical and physical structure of both an enveloped and a nonenveloped virus 5. Discuss the differences between the types of virus multiplication cycle 6. Classify the different species of viruses and the disease that each can cause 7. Cite different laboratory procedures in identifying viruses 8. Discuss the mechanism of interferon 9. Identify measures to prevent the spread of viral infections 10. Define a bacteriophage 11. Define an Oncogene and an oncovirus Lecture - Discussion with use of transparencies and power point presentation Assessment Tools > Quizzes > Recitation > Assignements Resources > Microbiology for the Health Sciences, Burton p. 72-85; 447-514 Meetings End of 12th Topi Learning Competencies c vi. Bunyavirus 13. Explain the pathogenicity, signs, and vii. Coronavirus symptoms of common viral diseases viii. Deltavirus 14. Identify nursing interventions in the ix. Calcivirus prevention, treatment, and x. Filovirus rehabilitation of patients with a viral xi. Retrovirus infection H. Laboratory and Diagnostic Procedures I. Interferon and Interference Phenomenon J. Preventive Measures against Viral Infections K. Bacterial Viruses L. Viruses and Cancer M. Common Viral Diseases 1. Skin 2. Ears 3. Respiratory System 4. Digestive System 5. Genito-Urinary System 6. Cardiovascular and Lymphatic System 7. Nervous System 8. Acquired Immuno Deficiency Syndrome MIDTERM EXAMINATION 13th - 15th week VII. Bacteriology A. Cell Morphology B. Structure C. Bacterial Arrangements D. Motility E. Nutritional requirements F. Atmospheric requirements G. Temperature requirements H. Staining Procedures 1. Gram Straining 2. Acid-Fast Staining At the end of the unit, the student should be able to: 1. Draw and label the different parts of a typical bacterium 2. List various ways in which bacteria can be classified or categorized 3. Differentiate among the types of bacteria according to their morphology 4. Differentiate among the types of Outcomes/Stude nt Lecture - Discussion with use of transparencies and power point presentation Assessment Tools > Quizzes > Recitation > Assignements Resources > Microbiology for the Health Sciences, Burton p. 85-103; 447-514 Meetings Topi c I. Gram Positive Bacteria 1. Staphylococci 2. Streptococci 3. Spore-forming Bacilli 4. Non Spore-forming Bacilli J. Gram Negative Bacteria 1. Neisseriae species 2. Enterobacteriaceae species 3. Pseudomonad species 4. Vibrio species 5. Helicobacter species 6. Campylobacter species 7. Hemophilus species 8. Bordetella species 9. Yersinia species 10. Pasteurella species K. Unique and Unusual Bacteria 1. Mycobacterium species 2. Spirochetes 3. Mycoplasma species 4. Rickettsial species 5. Chlamydiae species 16th - 18th week IX. Parasitology A. Parasitic Protozoa 1. Classification as to Movement 2. Protozoal Infections a. Skin b. Eyes c. Gastro-intestinal Tract d. Genito-urinary Tract e. Circulatory System f. Central Nervous System B. Helminths 1. Life Cycle 2. Classification Learning Competencies Outcomes/Stude nt 5. Differentiate among the types of bacteria according to their nutritional requirements 6. Differentiate among the types of bacteria according to their atmospheric requirements 7. Differentiate among the types of bacteria according to their temperature requirements 8. Differentiate among the types of bacteria according to their gram stain 9. Identify several important bacterial diseases of humans 10. Correlate a particular disease with its causative bacterial agent, signs and symptoms, reservoirs, modes of transmission, and nursing interventions 1. Enumerate and differentiate the types of parasites 2. Classify parasitic protozoa according to movement 3. List the life cycle of helminths in proper order 4. Classify and differentiate helminths 5. Identify arthropods capable in transmitting an infection 6. Classify the different types of Lecture - Discussion with use of transparencies and power point presentation Assessment Tools > Assignements Resources > Microbiology for the Health Sciences, Burton p. 517-536 Meetings Topi c a. Nematodes b. Platyhelminthes i. Cestodes ii. Trematodes 3. Common Helminthic Diseases C. Arthropods 1. Classification a. Insects b. Arachnids c. Crustaceans Learning Competencies Outcomes/Stude nt 8. Explain the pathogenicity, signs, and symptoms of common parasitic diseases 9. Identify nursing interventions in the prevention, treatment, and rehabilitation of patients with a parasitic infection FINAL EXAMINATION End of 18th VII. Course Outline I. Introduction to Microbiology and Parasitology A. Definition 1. Microbiology 2. Parasitology B. Types and Relationships 1. Pathogenic microorganisms 2. Non pathogenic microorganisms 3. Opportunistic microorganisms C. Importance D. Microbiology Subprovinces 1. Bacteriology 2. Phycology 3. Protozoology 4. Mycology 5. Virology E. Pioneers in the science of microbiology II. Cytology A. Classification B. Prokaryotic Cell Structure C. Eukaryotic Cell Structure D. Comparing Prokaryotic and Eukaryotic Cells 2 1/2 hours 5 hours Assessment Tools Resources E. Reproduction of organisms III. Pathogenesis of Infectious Disease A. Terminologies 1. Infection and Infectious Disease 2. Localized and Systemic Infections 3. Acute, Subacute, and Chronic Diseases 4. Signs and Symptoms of Diseases 5. Clinical and Subclinical Diseases 6. Primary and Secondary Infections B. Periods/Phases in the course of an Infectious Disease C. Steps in Pathogenesis D. Virulence and Virulence Factors 5 hours IV. Microbial Ecology A. Symbiosis B. Indigenous Microflora C. Epidemiology 1. Terminologies a. Communicable Disease b. Contagious Disease c. Non-communicable Disease d. Zoonotic Disease e. Incidence f. Prevalence g. Mortality h. Sporadic Disease i. Endemic Disease j. Epidemic Disease k. Pandemic Disease D. Chain of Infection 1. Pathogen 2. Reservoir 3. Portal of exit 4. Mode of Transmission 5. Portal of Entry 6. Susceptible Host 5 hours V. Host Defense Mechanisms A. Non Specific Host Defense Mechanism 1. First Line of Defense 2. Second Line of Defense B. Specific Host Defense Mechanism 1. Comparing Antibody Mediated and Cell Mediated Immunity 2. Acquired Immunity 3. Antibody Mediated Immunity 4. Cell mediated Immunity 5. Hypersensitivity Reaction 10 hours VI. Mycology A. Definition B. Characteristics C. Structure D. Reproduction E. Types F. Classification G. Mycoses/Fungal Infections 1. Superficial Mycosis 2. Systemic Mycosis 5 hours VII. Virology A. Definition B. Nature and Characteristic C. Morphology and Size D. Naming of Viruses E. Virus Multiplication Cycle F. Pathogenicity and Cytopathic Effect G. Classification 1. Deoxyribovirus 2. Ribovirus H. Laboratory and Diagnostic Procedures I. Interferon and Interference Phenomenon J. P Infections K. Bacterial Viruses L. Viruses and Cancer 20 hours M. Common Viral Diseases 1. Respiratory System 2. Skin 3. Ears 4. Digestive System 5. Genito-Urinary System 6. Cardiovascular and LymphaticSystem 7. Nervous System 8. Acquired Immuno Deficiency Syndrome (AIDS) VIII. Bacteriology A. Cell Morphology B. Structure C. Bacterial Arrangements D. Motility E. Nutritional requirements F. Atmospheric requirements G. Temperature requirements H. Staining Procedures I. Gram Positive Bacteria J. Gram Negative Bacteria K. Unique and Unusual Bacteria L. Common Bacterial Diseases 1. Respiratory System 2. Digestive System 3. Skin and Mucous Membrane 4. Neuro-muscular and Blood Diseases 5. Genito-urinary tract 15 hours IX. Parasitology A. Parasitic Protozoa 1. Classification as to Movement 2. Protozoal Infections a. Amoebiasis b. Trichomoniasis c. Malaria d. African Trypanosomiasis e. American Trypanosomiasis 15 hours f. Leishmaniasis g. Toxoplasmosis h. Primary Amoebic Meningoencephalitis i. Amoebic Conjunctivitis j. Giardiasis k. Cryptosporidiosis l. Cyclosporiasis m. Balantiditis B. Helminths 1. Life Cycle 2. Classification a. Nematodes b. Platyhelminthes 3. Common Helminthic Diseases C. Arthropods 1. Classification 2. Common Arthropod Infestations VII. Course Requirements 1. Attendance Class attendance is essential to success in the course. The student is responsible to obtain all assignments, handouts, and announcements which are made during the class period. Attendance is required for all exams. Special permission must be obtained for any exceptions. Exceptions to required attendance will be made for campus-sponsored activities, illness verified by a note from a physician or nurse, death in the family. 2. Written Examinations: a. Prelim b. Midterm c. Final 3. Quizzes, Assignments and Internet Searches VIII. Grading System Relative Weight of the Class Standing (Prelim, Midterm and Final) = 7 Relative Weight of the Major Exam (Prelim, Midterm and Final) = 3 Percent Average Passing = 65% transmuted to 75% Transmutation Table Average 97 – 100 94 – 96 91 – 93 88 – 90 85 – 87 82 – 84 79 – 81 76 – 78 75 Below 75 Failure due to absences Unauthorized Withdrawal Dropped Point-Grade 1.00 1.25 1.50 1.75 2.00 2.25 2.50 2.75 3.00 5.00 6.00 8.00 9.00 (*The Department employs a 65% passing percentage which is transmuted into 75%. A separate transmutation table is used to convert the raw scores for class standing and major exams.) Formulas: Midterm Average = 70% (Class Standing) + 30% (Major Exam Average) Class Standing = (CS-P + CS-M) /2 *CS-P = Class Standing in the Prelim Period (transmuted total score/100) *CS-M = Class Standing in the Midterm Period (transmuted total score/100) Major Exam Average = (P + M) /2 *P = Student’s Transmuted Prelim Exam Score/100 *M = Student’s Transmuted Midterm Exam Score/100 Final Average = 70% (Class Standing) + 30% (Major Exam Average) Class Standing = (CS-P + CS-M + CS-F) /3 *CS-F = Class Standing in the Final Period (transmuted total score/100) Major Exam Ave = (P + M + F) /3 *F = Student’s Transmuted Midterm Exam Score/100 IX. References: Microbiology for the Health Sciences, eighth edition; G. Burton, P. Engelkirk; 2007 Microbiology, A Human Perspective; Fourth edition; Nester, Anderson, Roberts, Pearsal, Nester; 2004 Microbiology, An Introduction; Eight edition; Tortora, Funke, Case; 2004 Alcamo's Fundamentals of Microbiology; seventh edition; Philippine edition; J. Pommerville; 2006 Introduction to Microbiology, A Case History Approach; third edition; Ingraham, Ingraham; 2004 Microbiology and Parasitology for the Health Professionals; Fisrt edition; Bautista; 2006 Microbiology and Parasitology, Laboratory Manual for the Health Sciences; second edition; Ho, am, Verbo; 2006 Essentials of Anatomy and Physiology, Sixth Edition; Seeley, Stephens, Tate; 2007 www.kcom.edu/faculty/chamberlain www.health.state.ny.us/diseases/communicable