Enzyme Notes Activation Energy: What are the two factors that affect enzymes? 1. 2. Define Catalyst: Define Enzyme: Substrates bind to an enzyme at certain places called active sites. The enzyme brings substrates together and weakens their bonds. The catalyzed reaction forms a product that is released from the enzyme. Which macromolecule can enzymes be categorized with? What effect does an enzyme have on activation energy? Why does temperature affect enzymes so much? Enzymes end in –ASE (ex: lactase, polymerase, helicase, etc.)