16 8 principles of success. Harry Sandhu 21 How can we track body composition changes with minimal technology and resources? By Alan Aragon Copyright © May 1st, 2020 by Alan Aragon Home: www.alanaragon.com Correspondence: support@alanaragon.com 2 Carbohydrate for endurance performance: research update on the fundamentals. By Alan Aragon 8 Mycoprotein ingestion stimulates protein synthesis rates to a greater extent than milk protein in rested and exercised skeletal muscle of healthy young men: a randomized controlled trial. Monteyne AJ, Coelho MO, Porter C, et al. [published online ahead of print, 2020 May 21]. Am J Clin Nutr. 2020;nqaa092. [PubMed] 11 Meat and mental health: a systematic review of meat abstention and depression, anxiety, and related phenomena. Neelakantan N, Seah JYH, van Dam RM. Circulation. 2020 Mar 10;141(10):803-814. [Taylor & Francis] 13 A plant-based, low-fat diet decreases ad libitum energy intake compared to an animalbased, ketogenic diet: An inpatient randomized controlled trial. Hall KD, Guo J, Courville AB, Boring J, Brychta R, Chen KY, et al. [Internet]. NutriXiv; 2020. [NutriXiv] Alan Aragon’s Research Review – May 2020 [Back to Contents] Page 1 Carbohydrate for endurance performance: research update on the fundamentals. By Alan Aragon _______________________________________________ Historical overview Carbohydrate has been referred to as “the largest single determinant of ensuring optimal performance during prolonged endurance events” aside from genetic capacity and training [1]. The ergogenic benefit of carbohydrate was published in the scientific literature as far back as 1920 [2]. Subsequent milestones in timeline of carbohydrate research include the 1960’s showing a clear relationship between glycogen availability and endurance capacity, the 1980’s showing that performance was increased via carbohydrate consumption during exercise [3]. The early 2000’s began a new era of research investigating the finer details of carbohydrate amount and type consumed during exercise to optimize performance at various durations. Novel strides were made in the understanding of the role of multiple transportable carbohydrate intake. The most recent decade spawned larger investigative strides in carbohydrate periodization; the strategic manipulation of carbohydrate availability for enhancing endurance performance. levels, also referred to as glycogen supercompensation. The classic carbohydrate loading model developed by Bergström et al [6] involves 3-4 days of glycogen depletion (10-100 g CHO/day; 5-15% of total kcal) coupled with exhaustive exercise, followed by 3-4 days of carb-loading (500-600 g CHO/day; 70% of total kcal or more) and reducing training volume – also called tapering. Although carbohydrate loading has effectively resulted in glycogen supercompensation, endurance performance advantages compared to control conditions are unlikely to occur in events that do not exceed 90 minutes [7]. Subsequent models aimed to minimize the adverse effects on mood seen by depletion phases by focusing more on tapering and a more linear increase in carbohydrate intake. Contemporary carbohydrate loading recommendations omit the depletion phase, and consist of a loading phase ranging from 8-12 g CHO/day for 1-3 days prior to competition, while training volume is tapered [8,9]. In the days preceding competition, carbohydrate loading is a technique used to attain supernormal glycogen storage In the final 4 hours preceding competition, the objective is to maximize levels of muscle and liver glycogen. After an overnight fast, liver glycogen stores can be reduced by as much as 80% [10]. This illuminates the importance of relatively immediate pre-exercise carbohydrate intake in common scenarios where competition initiates in the morning. However, there is a lack of consensus on amount and type of pre-exercise carbohydrate feeding within this timeframe due to the wide variability of individual circumstances. Large carbohydrate doses (~200-300 g) ingested 2-4 hours pre-exercise has been shown to enhance time trial performance and increase time to exhaustion [10,11]. Carbohydrate ingested within 1 hour pre-exercise has typically been dosed at approximately 1 g/kg, yielding a mix of results leaning toward the null [10]. Concern has been raised over the potential for “rebound hypoglycemia” during exercise when carbohydrate is ingested during this timeframe. However, the collective literature has not indicated this fluctuation in glycemia to threaten performance. The latter is illustrated by the largest metaanalysis on this topic to date, where Burdon et al [12] found no clear benefit of low- versus high-glycemic index pre-exercise meals for endurance performance. It should be noted that there is a lack of research directly comparing carbohydrate ingestion 2-4 hours versus 1 hour or less before exercise. In sum, ergogenic benefit is possible from a carbohydrate dose ranging 1-4 g/kg within the 4-hour window preceding higher-intensity (≥ 70% VO2max) Alan Aragon’s Research Review – May 2020 [Back to Contents] Pre-exercise Given the crucial importance of glycogen availability to endurance performance, the main role of pre-exercise carbohydrate intake is to “top off” or maximize glycogen stores. Therefore, the best attempts at optimizing preexercise carbohydrate intake cannot necessarily compensate for an insufficient chronic intake of total daily carbohydrate. Current position stands recommend daily carbohydrate intakes ranging 6-12 g/kg for competitive endurance sports involving daily training volumes ranging 3-6 hours per day [4,5]. When viewing pre-exercise carbohydrate intake as pre-competition intake, it can be viewed as three separate phases: the days preceding the competition, within approximately 4 hours, and within 1 hour prior to competition. Page 2 events exceeding 90 minutes [8]. This represents a wide range of possibilities, so personal trial/error is important for individualizing protocols to optimize results. During exercise Position stands converge on a during-exercise carbohydrate dosing range of 30-60 g/hour for endurance events that last 1 hour or longer [8,13]. The 30 g lower-end figure is not a magic number, nor is it an objective lower dosing threshold of effectiveness. Smith et al [14] found that dosing as low as 15 g/hour improved 20-km cycling time trial performance. On the upper end of the spectrum, 90 g/hour has been recommended for endurance events exceeding 2.5 hours [3,13]. A multicenter trial by Smith et al [16] examined the effect of 12 different carbohydrate doses in 10-gram increments (10-120 g/hour) found that cycling performance (2 hours at 70.8% VO2max followed by a 20-km time trial) progressively decreased as carbohydrate ingestion rates increased above 78 g/hour. It is important to bear in mind that the research (and thus the recommendations) on during-exercise carbohydrate intake is confounded by the testing of subjects in an overnight-fasted state, which is not representative of realworld race conditions. Colombani et al [17] aimed to examine real-world conditions in a systematic review that only included studies involving subjects in the postprandial/fed-state, in trials whose testing involved a fixed distance, fixed time, or fixed amount of work, or a submaximal exercise followed by a time trial – rather than a time-to-exhaustion model. It was concluded that carbohydrates ingested prior to, or during exercise would not likely enhance performance of bouts less than 70 minutes, and “a possible but not compelling ergogenic effect” with durations longer than 70 minutes. Following up on Colombani et al’s work, a meta-analysis by Pöchmüller et al [18] used similar inclusion criteria (a meal consumed 2-4 hours prior to time trial-type testing) and found that trained male cyclists, carbohydrate a 6-8% carbohydrate solution (also containing electrolytes) had ergogenic benefit for bouts longer than 90 minutes. Taken together, these findings indicate that immediate pre- or during-exercise carbohydrate ingestion are not likely to enhance performance in postprandial conditions unless the bout exceeds 70-90 minutes. erroneously built), The specific types of carbohydrate can impact performance through different mechanisms, depending on the nature of the bout. Glucose ingestion was recommended in earlier literature, but eventually, enhanced endurance performance was found from ingesting a combination of glucose and fructose. Glucose and fructose co-ingestion can increase the intestinal absorption rate of each monosaccharide (and thus total carbohydrate absorption) by utilizing different transporters (GLUT5 and SGLT1). This has been called the ‘multiple transportable carbohydrate’ model, where improved absorption leads to increased fuel delivery working muscle [19]. In addition to a gut/absorption-based mechanism driving the superior effects of glucose-fructose coingestion, it is also possible that hepatic production of lactate facilitates increased carbohydrate oxidation rates associated with higher work capacity. The preponderance of evidence supports the consumption of a glucose:fructose ratio ranging 1-2:1 during exercise, at a rate of 1.3-2.4 g/minute for maximizing 2.5-3.0-hour endurance performance compared to ingesting a single saccharide alone [20]. The advantage of the multiple transportable carbohydrate model might not be applicable to shorter durations. A systematic review by Stellingwerff and Cox [21] concluded that in events less than 60 minutes (nonglycogen-dependent conditions), it is possible that oral receptor exposure to carbohydrate, via either mouth rinse or oral consumption, stimulates the CNS reward centers, leading to enhanced performance. In contrast, events that are greater than 60 minutes where conditions where glycogen availability becomes a limiting factor, multiple transportable carbohydrate intake is warranted. The use of carbohydrate mouth rinse for enhancing endurance performance remains an interesting yet unresolved area of study. A meta-analysis Brietzke et al [22] found that the use of carbohydrate mouth rinse increased mean power output in cycling trials, but failed to improve time to complete the trials compared to placebo. Post-exercise Setting aside the issue of fed versus fasted performance testing (the latter upon which most recommendations are Post-exercise carbohydrate ingestion in the context of endurance performance is of utmost importance when there is time-urgency of restocking depleted glycogen stores. Classic work by Ivy [23] was perhaps the first to demonstrate the temporal impact of carbohydrate intake on Alan Aragon’s Research Review – May 2020 [Back to Contents] Page 3 post-exercise glycogen resynthesis after depletion. A 30minute delay (as opposed to two hours) of ingesting carbohydrate (2 g/kg) yielded ~50% faster glycogen repletion by the end of a 4-hour period. Jentjens and Jeukendrup [24] recommended that purposeful tactics to expedite glycogenesis should be employed when there are 8 hours or less between endurance events. Muscle glycogen synthesis rates are maximized when carbohydrate is consumed at 1.0-1.85 g/kg are consumed immediately post-exercise and at 15-60 minute intervals thereafter, for 3-5 hours. For the goal of maximizing rates of post-exercise glycogen resynthesis, the collective findings indicate immediate consumption of 1.2 g/kg/hour for 4-6 hours post-exercise. The type of carbohydrate consumed post-exercise can influence rate of glycogen resynthesis. The glycemic index (GI) of a given food is a measure of its ability to raise blood glucose levels, and has therefore been considered a reflection of its availability and effectiveness at restocking glycogen. High-GI carbohydrate sources have thus been recommended in the post-exercise period under urgent timeframes to recover between endurance exercise bouts [24]. Glucose has a high GI while fructose has a low GI, so traditionally, glucose and glucose polymers have been the prime targets of post-exercise endurance recovery. Indeed, direct comparison has shown superior glycogenic effects of post-exercise glucose versus fructose ingestion in isolation [25] and within mixed meals [26]. Interestingly, sucrose (a disaccharide composed of an even combination of glucose and fructose, with a moderate GI), has been shown to replenish glycogen at a similar rate to glucose when substantial amounts (≥1.2 g/kg/hour were consumed [27], while minimizing the gastrointestinal distress. maintaining a high carbohydrate intake, without specific timing relative to the exercise bout. Illustrating this, Starling et al [32] reported that after 24 hours, an intake of 9.8 g/kg restored 93% of the muscle glycogen used during a prior 2-hour cycling bout at 65% VO2max, while a lowcarbohydrate intake (1.9 g/kg) restored only 13%. There was no specific carbohydrate timing protocol aside from evenly distributed energy intake at breakfast, lunch, and dinner. Along these lines, Friedman et al [33] reported that complete muscle glycogen resynthesis after prolonged moderate-intensity exercise is possible in 24 hours if approximately 500-700 g of carbohydrate is consumed. Individuals who do not have immediate glycogen repletion requirements can relax their timing tactics, lift the emphasis off of high-GI foods, and shift the focus on total daily intake. On the note of flexibility, physical form of the carbohydrate source (liquid versus solid) has shown a lack of influence glycogen synthesis [24], but this is when consumed in amounts ranging 0.75-0.85 g/kg/hour. If consumed in amounts known to maximize rates of glycogen synthesis (~1.2 g/kg/hour), it is likely that the liquid form would be faster-acting, in addition to providing hydration. Emerging carbohydrate periodization strategies It is noteworthy that a high-molecular weight/lowosmolality carbohydrate (brand name Vitargo) has outperformed glucose monomers and polymers in trials examining rate of gastric emptying [28], rate of glycogen resynthesis [29], work output during a 15-minute cycling time trial [30], and power output during explosive resistance exercise preceded by glycogen depletion [31]. In light of these findings, it is worth reiterating that the speed of glycogen replenishment is of variable importance. Not all competitions involve the threat of glycogen depletion more than once in a day. Full glycogen repletion after depletion is possible within 24 hours by simply Jeukendrup [34] recently defined periodized nutrition as “the strategic combined use of exercise training and nutrition, or nutrition only, with the overall aim to obtain adaptations that support exercise performance.” There are a wide array of manipulations involving variations on the theme of training and/or recovering with low versus high exogenous and/or endogenous carbohydrate availability [35]. The “Train High” model can be into three variants: 1) high glycogen levels; 2) high exogenous carbohydrate; 3) high glycogen & exogenous carbohydrate. The latter variant has the strongest scientific support, and is thus reflective of the recommendations of the authoritative consensus statements and position stands [4,5,8]. The “Train Low” model can also be divided into three variants: 1) low glycogen levels; 2) fasted training; 3) fasted with low exogenous carbohydrate. Training with low carbohydrate availability has the potential to increase the activation of key cell signaling kinases and transcription factors which can result in mitochondrial biogenesis and the upregulation of lipid metabolism, thereby potentially improving exercise capacity. However, Train Low strategies (and carbohydrate restriction in general) should Alan Aragon’s Research Review – May 2020 [Back to Contents] Page 4 be used with caution due to risks including impaired exercise performance via decreased exercise economy [36] and impaired glycogen utilization [37], which could be an unintended a result of aiming to increase fat oxidation [38]. Other caveats of the Train Low variants include reduced training quality, increased risk of overreaching, and compromised immune response. More recent models of carbohydrate periodization include the “Recover Low/Sleep Low” variant [39], involving purposeful restriction of post-exercise carbohydrate intake to delay glycogen resynthesis. Post-exercise protein supplementation promoted muscle protein synthesis while preserving the effects of the low carbohydrate availability. 10 km running performance and submaximal cycling efficiency were improved compared to the non-periodized condition. Impey et al [40] demonstrated the performanceenhancing of a model they call “fuel for the work required” – which combines elements of the Train Low variants, but adds the twist of high carbohydrate availability for higher-intensity work, and low carbohydrate availability (and high amino acid availability) for low-intensity/non-exhaustive work. In contrast, Gejl et al [41] tested a periodized protocol designed to provide ample glycogen availability for highintensity bouts, and periodic carbohydrate restriction for the lower-intensity bouts. Despite the strategic manipulation of carbohydrate, no endurance performance advantage was seen compared to a non-periodized carbohydrate intake. carbohydrate intake (glucose: fructose at a 1-2:1 ratio) in a 608% carbohydrate-electrolyte solution is warranted during bouts exceeding 60 minutes. To maximize rates of post-exercise glycogen resynthesis (in cases where there is an urgent timeframe), the collective evidence indicates immediate consumption of 1.2 g/kg/hour for 4-6 hours post-exercise. Carbohydrate periodization models are in a relatively early stage of evolution, with the results thus far being mixed but promising. It is likely that the better the protocols can be tailored to individual preference, gastric tolerance, and performance goals – the closer they can come to optimization. REFERENCES Current position stands recommend daily carbohydrate intakes ranging 6-12 g/kg for competitive endurance sports. Contemporary carbohydrate loading recommendations consist of a loading phase ranging from 8-12 g CHO/day for 1-3 days prior to competition, while training volume is reduced/tapered. Carbohydrate intake ranging 14 g/kg within the 4-hour window pre-exercise can benefit higher-intensity events exceeding 90 minutes. It is unclear whether carbohydrate ingested within 1 hour pre-exercise imparts ergogenic benefits, especially in the context of earlier feeding. Immediate pre- or during-exercise carbohydrate ingestion are not likely to enhance performance in postprandial (fed) conditions unless the bout exceeds 70-90 minutes. Multiple transportable 1. Stellingwerff T, Cox GR. Systematic review: carbohydrate supplementation on exercise performance or capacity of varying durations. Appl Physiol Nutr Metab . 2014 Sep;39(9):998-1011. [PubMed] 2. Krogh A, Lindhard J. The relative value of fat and carbohydrate as sources of muscular energy: with appendices on the correlation between standard metabolism and the respiratory quotient during rest and work. Biochem J. 1920;14(3-4):290‐363. [PubMed] 3. Jeukendrup A. A step towards personalized sports nutrition: carbohydrate intake during exercise. Sports Med. 2014 May;44 Suppl 1(Suppl 1):S25-33. [PubMed] 4. Kerksick CM, Wilborn CD, Roberts MD, Smith-Ryan A, Kleiner SM, Jäger R, et al. ISSN exercise & sports nutrition review update: research & recommendations. J Int Soc Sports Nutr. 2018 Aug 1;15(1):38. [PubMed] 5. Thomas DT, Erdman KA, Burke LM. American College of Sports Medicine Joint Position Statement. Nutrition and Athletic Performance [published correction appears in Med Sci Sports Exerc. 2017 Jan;49(1):222]. 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Glucose-fructose ingestion and exercise performance: The gastrointestinal tract and beyond. Eur J Sport Sci. 2017;17(7):874‐884. [PubMed] Rowlands DS, Houltham S, Musa-Veloso K, Brown F, Paulionis L, Bailey D. Fructose-Glucose Composite Carbohydrates and Endurance Performance: Critical Review and Future Perspectives. Sports Med. 2015;45(11):1561‐1576. [PubMed] Stellingwerff T, Cox GR. Systematic review: Carbohydrate supplementation on exercise performance or capacity of varying durations. Appl Physiol Nutr Metab. 2014;39(9):998‐1011. [PubMed] Brietzke C, Franco-Alvarenga PE, Coelho-Júnior HJ, Silveira R, Asano RY, Pires FO. Effects of Carbohydrate Mouth Rinse on Cycling Time Trial Performance: A Systematic Review and MetaAnalysis [published correction appears in Sports Med. 2019 Feb 22;:]. Sports Med. 2019;49(1):57‐66. [PubMed] Ivy JL. Glycogen resynthesis after exercise: effect of carbohydrate intake. Int J Sports Med. 1998;19 Suppl 2:S142‐S145. [PubMed] Jentjens R, Jeukendrup A. Determinants of postexercise glycogen synthesis during short-term recovery. Sports Med. 2003;33(2):117‐144. [PubMed] Conlee RK, Lawler RM, Ross PE. Effects of glucose or fructose feeding on glycogen repletion in muscle and liver after exercise or fasting. Ann Nutr Metab. 1987;31(2):126‐132. [PubMed] Rosset R, Lecoultre V, Egli L, et al. Postexercise repletion of muscle energy stores with fructose or glucose in mixed meals. Am J Clin Nutr. 2017;105(3):609‐617. [PubMed]\ Gonzalez JT, Fuchs CJ, Betts JA, van Loon LJ. Glucose Plus Fructose Ingestion for Post-Exercise Recovery-Greater than the Sum of Its Parts?. Nutrients. 2017;9(4):344. [PubMed] Leiper JB, Aulin KP, Söderlund K. Improved gastric emptying rate in humans of a unique glucose polymer with gel-forming properties. Scand J Gastroenterol. 2000;35(11):1143‐1149. [PubMed] Piehl Aulin K, Söderlund K, Hultman E. Muscle glycogen resynthesis rate in humans after supplementation of drinks containing carbohydrates [Back to Contents] Page 6 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. with low and high molecular masses. Eur J Appl Physiol. 2000;81(4):346‐351. [PubMed] Stephens FB, Roig M, Armstrong G, Greenhaff PL. Post-exercise ingestion of a unique, high molecular weight glucose polymer solution improves performance during a subsequent bout of cycling exercise. J Sports Sci. 2008;26(2):149‐154. [PubMed] Oliver JM, Almada AL, Van Eck LE, et al. Ingestion of High Molecular Weight Carbohydrate Enhances Subsequent Repeated Maximal Power: A Randomized Controlled Trial. PLoS One. 2016;11(9):e0163009. [PubMed] Starling RD, Trappe TA, Parcell AC, Kerr CG, Fink WJ, Costill DL. Effects of diet on muscle triglyceride and endurance performance. J Appl Physiol (1985). 1997;82(4):1185‐1189. [PubMed] Friedman JE, Neufer PD, Dohm GL. Regulation of glycogen resynthesis following exercise. Dietary considerations. Sports Med. 1991;11(4):232‐243. [PubMed] Jeukendrup AE. Periodized Nutrition for Athletes. Sports Med. 2017;47(Suppl 1):51‐63. [PubMed] Burke LM, Hawley JA, Jeukendrup A, Morton JP, Stellingwerff T, Maughan RJ. Toward a Common Understanding of Diet-Exercise Strategies to Manipulate Fuel Availability for Training and Competition Preparation in Endurance Sport. Int J Sport Nutr Exerc Metab. 2018;28(5):451‐463. [PubMed] Burke LM, Ross ML, Garvican-Lewis LA, et al. Low carbohydrate, high fat diet impairs exercise economy and negates the performance benefit from intensified training in elite race walkers. J Physiol. 2017;595(9):2785‐2807. [PubMed] Stellingwerff T, Spriet LL, Watt MJ, et al. Decreased PDH activation and glycogenolysis during exercise following fat adaptation with carbohydrate restoration. Am J Physiol Endocrinol Metab. 2006;290(2):E380‐E388. [PubMed] Burke LM. Re-examining high-fat diets for sports performance: Did we call the 'nail in the coffin' too soon?. Sports Med. 2015;45 Suppl 1(Suppl 1):S33‐S49. [PubMed] Marquet LA, Brisswalter J, Louis J, et al. Enhanced Endurance Performance by Periodization of Carbohydrate Intake: "Sleep Low" Strategy. Med Sci Sports Exerc. 2016;48(4):663‐672. [PubMed] Alan Aragon’s Research Review – May 2020 40. Impey SG, Hearris MA, Hammond KM, et al. Fuel for the Work Required: A Theoretical Framework for Carbohydrate Periodization and the Glycogen Threshold Hypothesis. Sports Med. 2018;48(5):1031‐1048. [PubMed] 41. Gejl KD, Thams LB, Hansen M, et al. No Superior Adaptations to Carbohydrate Periodization in Elite Endurance Athletes. Med Sci Sports Exerc. 2017;49(12):2486‐2497. [PubMed] [Back to Contents] Page 7 registered at clinicaltrials.gov as 660065600. FUNDING SOURCE: Marlow Foods Ltd. Mycoprotein ingestion stimulates protein synthesis rates to a greater extent than milk protein in rested and exercised skeletal muscle of healthy young men: a randomized controlled trial. Monteyne AJ, Coelho MO, Porter C, et al. [published online ahead of print, 2020 May 21]. Am J Clin Nutr. 2020;nqaa092. [PubMed] BACKGROUND: Mycoprotein is a fungal-derived sustainable protein-rich food source, and its ingestion results in systemic amino acid and leucine concentrations similar to that following milk protein ingestion. Objective: We assessed the mixed skeletal muscle protein synthetic response to the ingestion of a single bolus of mycoprotein compared with a leucine-matched bolus of milk protein, in rested and exercised muscle of resistance-trained young men. METHODS: Twenty resistance-trained healthy young males (age: 22 ± 1 y, body mass: 82 ± 2 kg, BMI: 25 ± 1 kg·m-2) took part in a randomized, double-blind, parallel-group study. Participants received primed, continuous infusions of L-[ring-2H5]phenylalanine and ingested either 31 g (26.2 g protein: 2.5 g leucine) milk protein (MILK) or 70 g (31.5 g protein: 2.5 g leucine) mycoprotein (MYCO) following a bout of unilateral resistance-type exercise (contralateral leg acting as resting control). Blood and m. vastus lateralis muscle samples were collected before exercise and protein ingestion, and following a 4-h postprandial period to assess mixed muscle fractional protein synthetic rates (FSRs) and myocellular signaling in response to the protein beverages in resting and exercised muscle. RESULTS: Mixed muscle FSRs increased following MILK ingestion (from 0.036 ± 0.008 to 0.052 ± 0.006%·h-1 in rested, and 0.035 ± 0.008 to 0.056 ± 0.005%·h-1 in exercised muscle; P <0.01) but to a greater extent following MYCO ingestion (from 0.025 ± 0.006 to 0.057 ± 0.004%·h-1 in rested, and 0.024 ± 0.007 to 0.072 ± 0.005%·h-1 in exercised muscle; P <0.0001) (treatment × time interaction effect; P <0.05). Postprandial FSRs trended to be greater in MYCO compared with MILK (0.065 ± 0.004 compared with 0.054 ± 0.004%·h-1, respectively; P = 0.093) and the postprandial rise in FSRs was greater in MYCO compared with MILK (Delta 0.040 ± 0.006 compared with Delta 0.018 ± 0.005%·h-1, respectively; P <0.01). CONCLUSIONS: The ingestion of a single bolus of mycoprotein stimulates resting and postexercise muscle protein synthesis rates, and to a greater extent than a leucine-matched bolus of milk protein, in resistance-trained young men. This trial was Alan Aragon’s Research Review – May 2020 Strengths This is an interesting study from the ideological standpoint of the search for a plant-based, sustainable protein source that is on par quality-wise with the “big boys” such as dairy and flesh proteins. The subjects were recreationally active, and what the authors deemed to be “resistancetrained” (≥3 times per week for ≥3 months prior to participation). All subjects were provided with a standardized meal that was consumed ∼10.5 hours prior to the start of testing the following morning. This minimized the potential for confounding variability in circulating substrates that could have influenced the results. Limitations A limitation of all studies that measure acute response (in this case 4 hours after ingestion) is that questions remain as to the longer-term adaptations in body composition and other longitudinally determined outcomes. The uncertain applicability of short-term outcomes is illustrated by the mix of not-necessarily-predictable outcomes in studies on muscle growth. There are several such studies that challenge the presumptions surrounding muscle protein synthesis (MPS) in acute studies. Different protein types (plant vs. dairy) have shown similar effects on muscle hypertrophy in some studies,1-5 Superior hypertrophic effects of whey protein have been seen versus soy protein,6 and superior effects of pea have been seen versus whey.7 A recent meta-analysis by Messina et al8 included 9 studies (5 studies compared whey vs soy, 4 studies compared soy vs animal proteins including beef, milk, or dairy protein), and found that soy protein results in similar gains in strength & lean mass compared to whey. Despite the intentional objective of equating leucine in the protein doses compared, the greater total amount of protein (31.5 vs 26.2 g) and kcal (238 vs 108) in the mycoprotein product potentially confounds the comparison. Although leucine is a potent and independent driver of MPS,10,11 cofactors within the protein matrix have been implicated in superseding leucine’s isolated effects. For example, Churchward-Venne et al12 found that a 25 g dose of whey protein (containing 3 g leucine) elicited greater MPS than the 5-g leucine doses within the comparator treatments. [Back to Contents] Page 8 Comment/application References As shown above, the fungus-derived myocoprotein outperformed milk protein in the primary outcome, which was mixed skeletal muscle protein synthesis (MPS). The left side of the above (A) shows rested and exercised responses in the fasted and fed conditions. The right side (B) shows the change/increase in MPS in the rested and exercised conditions. In addition to MPS, 46 genes were analyzed for their mRNA expression. Only a single gene, (TRIM32) showed a significantly different response between nutritional conditions, greater in the milk protein compared with mycoprotein in the fed state. It's noteworthy that the greater kcal load in the mycoprotein treatment was due to slightly more protein, but significantly more fat (9 vs 0.3 g). There are at least a couple of examples in the literature of protein accompanied by fat being more acutely anabolic than protein alone. In a protein-matched comparison, van Vliet et al13 found that whole eggs elicited greater MPS than egg whites. Along similar lines, Elliot et al14 found that whole milk stimulated greater MPS than skim milk despite the latter having a higher protein dose. A more compelling comparison than the resent study would involve matched macronutrition between the treatments, and the use of a more common protein supplement such as whey protein to increase the external validity (real-world relevance) of the findings. Milk protein concentrate, which was used in this study, is vastly less common than whey. 1. Kalman D, Feldman S, Martinez M, Krieger DR, Tallon MJ. Effect of protein source and resistance training on body composition and sex hormones. J Int Soc Sports Nutr. 2007;4:4. Published 2007 Jul 23. [PubMed] 2. Haun CT, Mobley CB, Vann CG, et al. Soy protein supplementation is not androgenic or estrogenic in college-aged men when combined with resistance exercise training [published correction appears in Sci Rep. 2018 Aug 10;8(1):12221]. Sci Rep. 2018;8(1):11151. [PubMed] 3. Joy JM, Lowery RP, Wilson JM, et al. The effects of 8 weeks of whey or rice protein supplementation on body composition and exercise performance. Nutr J. 2013;12:86. [PubMed] 4. Mobley CB, Haun CT, Roberson PA, et al. Effects of Whey, Soy or Leucine Supplementation with 12 Weeks of Resistance Training on Strength, Body Composition, and Skeletal Muscle and Adipose Tissue Histological Attributes in College-Aged Males. Nutrients. 2017;9(9):972. [PubMed] 5. Denysschen CA, Burton HW, Horvath PJ, Leddy JJ, Browne RW. Resistance training with soy vs whey protein supplements in hyperlipidemic males. J Int Soc Sports Nutr. 2009;6:8. [PubMed] 6. Volek JS, Volk BM, Gómez AL, et al. Whey protein supplementation during resistance training augments lean body mass. J Am Coll Nutr. 2013;32(2):122‐135. [PubMed] 7. Banaszek A, Townsend JR, Bender D, Vantrease WC, Marshall AC, Johnson KD. The Effects of Whey vs. Pea Protein on Physical Adaptations Following 8Weeks of High-Intensity Functional Training (HIFT): A Pilot Study. Sports (Basel). 2019;7(1):12. [PubMed] Mycoprotein, made from Fusarium venenatum (a naturally occurring fungus, sold under the brand name of Quorn), is reported to have a meat-like texture, and a more environmentally friendly impact than beef and chicken.15 However, a potential barrier to the sustainable use of mycoprotein could be its cost. Per gram of protein, at Quorn costs 2-3 times as much as whey. Still, it shows promise as a First-World solution. :) 8. Messina M, Lynch H, Dickinson JM, Reed KE. No Difference Between the Effects of Supplementing With Soy Protein Versus Animal Protein on Gains in Muscle Mass and Strength in Response to Resistance Exercise. Int J Sport Nutr Exerc Metab. 2018;28(6):674‐685. [PubMed] 9. Fuchs CJ, Hermans WJH, Holwerda AM, et al. Branched-chain amino acid and branched-chain ketoacid ingestion increases muscle protein synthesis rates in vivo in older adults: a double-blind, randomized trial. Am J Clin Nutr. 2019;110(4):862‐872. [PubMed] Alan Aragon’s Research Review – May 2020 [Back to Contents] Page 9 10. Devries MC, McGlory C, Bolster DR, Kamil A, Rahn M, Harkness L, Baker SK, Phillips SM. Leucine, not total protein, content of a supplement is the primary determinant of muscle protein anabolic responses in healthy older women. J Nutr. 2018;148(7):1088–95. [PubMed] 11. Churchward-Venne TA, Breen L, Di Donato DM, et al. Leucine supplementation of a low-protein mixed macronutrient beverage enhances myofibrillar protein synthesis in young men: a double-blind, randomized trial. Am J Clin Nutr. 2014;99(2):276‐286. [PubMed] 12. Churchward-Venne TA, Breen L, Di Donato DM, et al. Leucine supplementation of a low-protein mixed macronutrient beverage enhances myofibrillar protein synthesis in young men: a double-blind, randomized trial. Am J Clin Nutr. 2014;99(2):276‐286. [PubMed] 13. van Vliet S, Shy EL, Abou Sawan S, et al. Consumption of whole eggs promotes greater stimulation of postexercise muscle protein synthesis than consumption of isonitrogenous amounts of egg whites in young men. Am J Clin Nutr. 2017;106(6):1401‐1412. [PubMed] 14. Elliot TA, Cree MG, Sanford AP, Wolfe RR, Tipton KD. Milk ingestion stimulates net muscle protein synthesis following resistance exercise. Med Sci Sports Exerc. 2006;38(4):667‐674. [PubMed] 15. Finnigan TJA, Wall BT, Wilde PJ, Stephens FB, Taylor SL, Freedman MR. Mycoprotein: The Future of Nutritious Nonmeat Protein, a Symposium Review. Curr Dev Nutr. 2019;3(6):nzz021. [PubMed] Alan Aragon’s Research Review – May 2020 [Back to Contents] Page 10 Meat and mental health: a systematic review of meat abstention and depression, anxiety, and related phenomena. Neelakantan N, Seah JYH, van Dam RM. Circulation. 2020 Mar 10;141(10):803-814. [Taylor & Francis] OBJECTIVE: To examine the relation between the consumption or avoidance of meat and psychological health and well-being. METHODS: A systematic search of online databases (PubMed, PsycINFO, CINAHL Plus, Medline, and Cochrane Library) was conducted for primary research examining psychological health in meatconsumers and meat-abstainers. Inclusion criteria were the provision of a clear distinction between meat-consumers and meat-abstainers, and data on factors related to psychological health. Studies examining meat consumption as a continuous or multi-level variable were excluded. Summary data were compiled, and qualitative analyses of methodologic rigor were conducted. The main outcome was the disparity in the prevalence of depression, anxiety, and related conditions in meat-consumers versus meat-abstainers. Secondary outcomes included mood and self-harm behaviors. RESULTS: Eighteen studies met the inclusion/exclusion criteria; representing 160,257 participants (85,843 females and 73,232 males) with 149,559 meat-consumers and 8584 meat-abstainers (11 to 96 years) from multiple geographic regions. Analysis of methodologic rigor revealed that the studies ranged from low to severe risk of bias with high to very low confidence in results. Eleven of the 18 studies demonstrated that meatabstention was associated with poorer psychological health, four studies were equivocal, and three showed that meat-abstainers had better outcomes. The most rigorous studies demonstrated that the prevalence or risk of depression and/or anxiety were significantly greater in participants who avoided meat consumption. CONCLUSION: Studies examining the relation between the consumption or avoidance of meat and psychological health varied substantially in methodologic rigor, validity of interpretation, and confidence in results. The majority of studies, and especially the higher quality studies, showed that those who avoided meat consumption had significantly higher rates or risk of depression, anxiety, and/or self-harm behaviors. There was mixed evidence for temporal relations, but study designs and a lack of rigor precluded inferences of causal relations. Our study does Alan Aragon’s Research Review – May 2020 not support meat avoidance as a strategy to benefit psychological health. FUNDING SOURCE: This study was funded in part via an unrestricted research grant from the Beef Checkoff, through the National Cattlemen’s Beef Association. The sponsor of the study had no role in the study design, data collection, data analysis, data interpretation, or writing of the report. Strengths Systematic reviews are considered to be a step up from narrative reviews in the evidence hierarchy due to an increased level of thoroughness and a higher degree of rigor in terms of quality assessment of the included literature. The authors followed the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guidelines for reporting in systematic reviews and meta-analyses,1 which is one of the field standards for bolstering transparency and reducing bias. The studies were ranked in five categories based on their score in a 100-point quality system. The review included 16 papers and a large total sample 160,257 participants (73,232 M & 85,843 F), over a broad age range (11-96 yrs). Limitations The authors acknowledged the following limitations of their review: ▪ ▪ ▪ ▪ ▪ Non-English language studies were excluded, which could potentially bias results in favor of Western patterns which typically include meat consumption. Nevertheless, the review included a large sample from China; so, this could significantly mitigate that limitation. A large number of papers with potentially useful data did not meet the inclusion criteria. However, this may have resulted in more focused & stronger evidence. Despite the high confidence in the finding that meat-abstention is linked to psychological disorders, the observational nature of the studies does not allow the demonstration of causation. Meat consumption is often inaccurately classified in national surveillance settings, and across languages. All studies relied on self-reported dietary intake, which is subject to a wide range of biases, inconsistencies, and inaccuracies. [Back to Contents] Page 11 ▪ Studies of lower-methodological quality utilized a single questionnaire (as opposed to multiple assessments of psychological outcomes) and/or a single tool for examining psychological symptoms over a short timeframe. 3. The ideological nature of this topic carries inherent potential for confounding. I’ll quote the manuscript’s eloquently put account: “These non-intentional biases in concert with the potential for a participant to intentionally misreport outcomes to support his or her ideological stances or religious beliefs may induce systematic and non-quantifiable errors when employing self-report protocols. As such, the oversampling of groups that are highly invested in their dietary regimes for health, religious, or ideologic concerns (e.g., animals rights) will lead to biased recruitment and extremely unreliable data.” 4. Comment/application 7. The main findings of this systematic review were what the authors stated as “clear evidence” associating meatabstention with higher risk of depression, anxiety, and selfharm. Associations with mood states, affective well-being, stress perception and quality of life were equivocal. Nevertheless, the present’s review’s findings present savory food for thought, as it presents a victory for advocates of meat consumption by showing that the majority of the existing research supports it for mental health.2-12 The research in opposition of this conclusion13,15 should not be ignored despite it being outweighed. This is a complicated topic since there are several classifications along the spectrum of vegetarianism (strict vegan, lacto-, lacto-ovo, lacto-ovo-pesco). There are also omnivores who only avoid singular types of meat (usually red meat). There are a multitude of diet & non-diet factors that contribute to mental health status. Abstaining from meat is one potential factor in a constellation of red flags. References 1. Liberati A, Altman DG, Tetzlaff J, Mulrow C, Gøtzsche PC, Ioannidis JP, Clarke M, Devereaux PJ, Kleijnen J, Moher D. The PRISMA statement for reporting systematic reviews and meta-analyses of studies that evaluate healthcare interventions: explanation and elaboration. BMJ. 2009 Jul 21;339:b2700. [PubMed] 2. Lavallee K, Zhang XC, Michalak J, Schneider S, Margraf J. Vegetarian diet and mental health: CrossAlan Aragon’s Research Review – May 2020 5. 6. 8. 9. 10. 11. 12. 13. 14. 15. sectional and longitudinal analyses in culturally diverse samples. J Affect Disord. 2019;248:147‐154. [PubMed] Perry CL, Mcguire MT, Neumark-Sztainer D, Story M. Characteristics of vegetarian adolescents in a multiethnic urban population. J Adolesc Health. 2001;29(6):406‐416. [PubMed] Neumark-Sztainer D, Story M, Resnick MD, Blum RW. Adolescent vegetarians. A behavioral profile of a school-based population in Minnesota. Arch Pediatr Adolesc Med. 1997;151(8):833‐838. [PubMed] Baines S, Powers J, Brown WJ. How does the health and well-being of young Australian vegetarian and semi-vegetarian women compare with nonvegetarians? Public Health Nutr. 2007;10(5):436‐442. [PubMed] Stokes N, et al. Vegetarian Diets and Mental Health in Adolescents with Anorexia Nervosa. anuary 2011Journal of Adolescent Health 48(2):S50-S50. [JAH] Lindeman M. The state of mind of vegetarians: Psychological well-being or distress? September 2010Ecology of Food and Nutrition 41(1):75-86. [EFN] Forestell CA, Nezlek JB. Vegetarianism, depression, and the five factor model of personality. Ecol Food Nutr. 2018;57(3):246‐259. [PubMed] Baş M, Karabudak E, Kiziltan G. Vegetarianism and eating disorders: association between eating attitudes and other psychological factors among Turkish adolescents. Appetite. 2005;44(3):309‐315. [PubMed] Hibbeln JR, Northstone K, Evans J, Golding J. Vegetarian diets and depressive symptoms among men. J Affect Disord. 2018;225:13‐17. [PubMed] Matta J, Czernichow S, Kesse-Guyot E, et al. Depressive Symptoms and Vegetarian Diets: Results from the Constances Cohort. Nutrients. 2018;10(11):1695. [PubMed] Michalak J, Zhang XC, Jacobi F. Vegetarian diet and mental disorders: results from a representative community survey. Int J Behav Nutr Phys Act. 2012;9:67. [PubMed] Beezhold BL, Johnston CS, Daigle DR. Vegetarian diets are associated with healthy mood states: a crosssectional study in seventh day adventist adults. Nutr J. 2010;9:26. [PubMed] Beezhold BL, Johnston CS. Restriction of meat, fish, and poultry in omnivores improves mood: a pilot randomized controlled trial. Nutr J. 2012;11:9. [PubMed] Beezhold B, Radnitz C, Rinne A, DiMatteo J. Vegans report less stress and anxiety than omnivores. Nutr Neurosci. 2015;18(7):289‐296. [PubMed] [Back to Contents] Page 12 A plant-based, low-fat diet decreases ad libitum energy intake compared to an animal-based, ketogenic diet: An inpatient randomized controlled trial. Hall KD, Guo J, Courville AB, Boring J, Brychta R, Chen KY, et al. BACKGROUND/PURPOSE: Competing models of obesity and its treatment often contrast the relative roles of dietary fat versus carbohydrate. Advocates of lowcarbohydrate diets posit that intake of high glycemic carbohydrates leads to elevated postprandial insulin thereby promoting body fat accumulation while increasing hunger and energy intake according to the carbohydrateinsulin model of obesity. Alternatively, proponents of lowfat diets argue that high fat intake promotes body fat storage due to passive overconsumption of energy resulting from the high energy density of dietary fat. DESIGN: To test these competing models, 20 adults without diabetes aged (mean±SE) 29.9±1.4 y with BMI=27.8±1.3 kg/m2 were admitted as inpatients to the NIH Clinical Center and randomized to consume ad libitum either a plant-based, low-fat (PBLF) diet (75.2% carbohydrate, 10.3% fat, non-beverage energy density = 1.1 kcal/g) or an animal-based, ketogenic, lowcarbohydrate (ABLC) diet (75.8% fat,10.0% carbohydrate, non-beverage energy density = 2.2 kcal/g) for two weeks followed immediately by the alternate diet for two weeks. Three daily meals plus snacks amounting to twice each subject’s estimated energy requirements were provided and subjects were instructed to eat as much or as little as desired. RESULTS: The PBLF diet resulted in substantially greater glucose and insulin levels whereas the ABLC diet led to increased blood ketones of ~3 mM which is thought to suppress appetite. However, ad libitum energy intake was 689±73 kcal/d lower during the PBLF diet as compared to the ABLC diet (p<0.0001) with no significant differences in appetite ratings or enjoyment of meals. CONCLUSIONS: These data challenge the veracity of the carbohydrate-insulin model of obesity and suggest that the PBLF diet had benefits for appetite control whereas the ABLC diet had benefits for lowering blood glucose and insulin. FUNDING SOURCE: This work was supported by the Intramural Research Program of the National Institutes of Health, National Institute of Diabetes & Digestive & Kidney Diseases. that restricted their targeted macronutrients (carbohydrate or fat) a maximum of 10% of total energy. This design eliminates the possibility that the targeted macronutrient differences were not disparate enough to reveal the benefit of either diet, which is a common confounder in this area of diet research. Another strength was the crossover design wherein each subject underwent each diet treatment. Appetite and palatability were assessed, and body composition was assessed via dual-energy X-ray absorptiometry (DXA). Energy expenditure was measured via respiratory chamber, and physical activity was assessed via accelerometer. Blood ketone levels were also assessed. Limitations The authors acknowledged that the study didn’t include a weight-maintenance run-in period or a washout period between the test diets. They further conceded that the main limitation was the controlled inpatient environment (as opposed to free-living conditions) which leaves questions about generalizing the results to the real world. The food environment (in terms of choices) was controlled so that the only choice available was how much of the presented foods and beverages could be consumed by the subjects. In addition, hypocaloric conditions for weight loss were not addressed; it remains to be seen whether the ad libitum/non-restrictive results apply to dieting. I would note that these acknowledged limitations can be considered strengths, as far as the research question & purpose go. I would add to this that the duration of each condition was only 2 weeks. This leaves wide-open questions about the longer-term effects of each diet. Comment/application Strengths This study was a fresh iteration of the low-carb versus low-fat battle. It’s the first inpatient study that measured ad libitum (as desired) food intake comparing two diets A number of interesting and unexpected results occurred. As shown above, energy intake was significantly lower in the plant-based low-fat diet (PBLF) by ~550-700 kcal/day. Alan Aragon’s Research Review – May 2020 [Back to Contents] Page 13 outperformed structured low-fat diets for the goal of weight/fat loss, at least in the short/mid-term.2-5 However, it should be noted that even in the long-term the at times statistically significant weight loss superiority of ketogenic diets still lack clinical or practical significance (approximately 0.9 kg more weight loss than low-fat comparators at 12-24 months).6 It should be noted that the present study’s low-carb diet was also a low-fiber condition (6.8 g/1000 kcal vs 29.9 g/1000 kcal). This leaves open questions about how results might have differed if the keto was also high-fiber. Dietary fiber has been consistently associated with weight loss7,8 and cardiovascular risk reduction.9 The present study is just too short to be much more than a hypothesis-generating conversation piece. Long-term comparisons thus far have shown little to no difference in low- versus high-carb comparisons. One of the most compelling examples is the 12-month DIETFITS trial by Gardner et al,10 which compared a ‘healthy’ low-fat with a ‘healthy’ low-carb diet which was strictly ketogenic for the first 8 weeks, with the allowance of a 5-15 g increase in carbs only as needed to arrive at what the subjects felt was the lowest sustainable dose. The DIETFITS trial found no significant difference in weight/fat loss between the diets. Furthermore, there was no influence of genotype pattern nor baseline insulin secretion on the results. References A particularly interesting aspect of this study’s design was the ad libitum nature of the diet assignments. Historically in the research, ad libitum ketogenic diets have largely 1. Rynders CA, Thomas EA, Zaman A, Pan Z, Catenacci VA, Melanson EL. Effectiveness of Intermittent Fasting and Time-Restricted Feeding Compared to Continuous Energy Restriction for Weight Loss. Nutrients. 2019 Oct 14;11(10) [PubMed] 2. Hashimoto Y, Fukuda T, Oyabu C, et al. Impact of low-carbohydrate diet on body composition: metaanalysis of randomized controlled studies. Obes Rev. 2016;17(6):499‐509. [PubMed] 3. Sackner-Bernstein J, Kanter D, Kaul S. Dietary Intervention for Overweight and Obese Adults: Comparison of Low-Carbohydrate and Low-Fat Diets. A Meta-Analysis. PLoS One. 2015;10(10):e0139817. [PubMed] 4. Bueno NB, de Melo IS, de Oliveira SL, da Rocha Ataide T. Very-low-carbohydrate ketogenic diet v. low-fat diet for long-term weight loss: a meta-analysis Alan Aragon’s Research Review – May 2020 [Back to Contents] Given that energy intake decreased in both groups but more so in PBLF, it’s not surprising to see the accompanying significant weight loss and fat loss, but more so in PBLF (shown above). With a bit of reflection on the possibility of glycogen reduction, it’s not too surprising to see the preservation of lean mass in PBLF as well, while a significant decrease in lean mass occurred in ABLC. Compounding the chagrin of low-carb/keto absolutists, these changes occurred despite higher blood glucose and insulin levels in PBLF. Adding insult to injury, these result were seen despite a lower protein intake in PBLF. Page 14 5. 6. 7. 8. 9. 10. of randomised controlled trials. Br J Nutr. 2013;110(7):1178‐1187. [PubMed] Hession M, Rolland C, Kulkarni U, Wise A, Broom J. Systematic review of randomized controlled trials of low-carbohydrate vs. low-fat/low-calorie diets in the management of obesity and its comorbidities. Obes Rev. 2009;10(1):36‐50. [PubMed] Ting R, Dugré N, Allan GM, Lindblad AJ. Ketogenic diet for weight loss. Can Fam Physician. 2018;64(12):906. [PubMed] Clark MJ, Slavin JL. The effect of fiber on satiety and food intake: a systematic review. J Am Coll Nutr. 2013;32(3):200‐211. [PubMed] Miketinas DC, Bray GA, Beyl RA, Ryan DH, Sacks FM, Champagne CM. Fiber Intake Predicts Weight Loss and Dietary Adherence in Adults Consuming Calorie-Restricted Diets: The POUNDS Lost (Preventing Overweight Using Novel Dietary Strategies) Study. J Nutr. 2019;149(10):1742‐1748. [PubMed] Bozzetto L, Costabile G, Della Pepa G, et al. Dietary Fibre as a Unifying Remedy for the Whole Spectrum of Obesity-Associated Cardiovascular Risk. Nutrients. 2018;10(7):943. [PubMed] Gardner CD, Trepanowski JF, Del Gobbo LC, et al. Effect of Low-Fat vs Low-Carbohydrate Diet on 12Month Weight Loss in Overweight Adults and the Association With Genotype Pattern or Insulin Secretion: The DIETFITS Randomized Clinical Trial [published correction appears in JAMA. 2018 Apr 3;319(13):1386] [published correction appears in JAMA. 2018 Apr 24;319(16):1728]. JAMA. 2018;319(7):667‐679. [PubMed] Alan Aragon’s Research Review – May 2020 [Back to Contents] Page 15 8 Principles of Success By Harry Sandhu _______________________________________________ If you asked 100 successful people on what goes into success, you will discover, the answers they give you, could easily be divided into 2 parts. Many of the things will be universally recognized, like Hard Work, Resilience etc. On the other hand, there will be many which are very unique and endeared by select few, for example the use of Self SWOT Analysis that I talk about below, might not be universally preached, but they still might resonate with many. Achieving success has no set, in stone blueprint. It’s a collection of Philosophies and Principles coming together, and adhered to with relentless consistency over time. As cliched as it might sound, you ultimately are a sum of your collective daily actions. I am going to share some of my beliefs and philosophies that are at the core of my belief system, and I believe have played a huge part in being where I am today. This is such an area, that if we start going deep into it, we easily could be here all day. However, to spare you all the agony of a 5 hour read, I am going to break each of my Philosophies into condensed, To the Point, Take Home Message. So, let’s start with the most common, and perhaps the oldest one that we all can safely agree on. 1: HARD WORK: We have often heard the phrase, “There is no alternative to hard work”. Well, I can’t make it any simpler or clearer. There indeed is no alternative to hard work. Now, this is nothing revolutionary that I have shared. However, things become a little more interesting from the next line. See, as important as Hard Work is, I have a very differing view to how I look at, it’s “importance” to most people. I’ll try to explain to you via a simple example. When someone says to me, “Oh, I work so hard”, that does not impress me 1 bit. This attitude of mine might surprise some of you. I am not dissing your efforts, not in the slightest. However, it’s nothing to glorify either. Alan Aragon’s Research Review – May 2020 The way I look at Hard Work, is like breathing. As breathing is to stay alive, likewise Hard Work is to success – one doesn’t happen without the other. Like you can’t stay alive without breathing, likewise you can’t become self – made successful person without hard work. So, that to me makes Hard Work as something nothing to glorify, but something that just needs to be and MUST be done. If you went out to look, 100% of the successful people will have insane work ethic, but what you will discover below is, they will often have all or at least lot of the below qualities too. And, that’s where the magic begins to happen. Remaining of the below principles are in no particular order of hierarchy, but all are needed in various degrees at various times. 2: Luck: Luck, to lot of people on the “surface” doesn’t belong anywhere in the vocabulary of successful people. However, let me give you a slightly different perspective than you might have be used to. Many times, where you are “born”, or which “country” you are born in, can seal your fate. For example, you can’t compare the opportunities most of the Western World countries present to anyone who’s born in those countries, versus, say someone born in Syria or war – torn Iraq. If you are born in a country like America, you already have fortune smiling brightly on you, and you have no excuse or reason to not make an absolute fist of your goals. For example, in a country like America (and most other developed countries), even the poorest of the poor have access to basic amenities like, clean water, electricity, shelter, food and above all, equal opportunity. However, on the other hand, even such basics can appear to be a luxury for many, and equal opportunity doesn’t even exist in many developing countries. However, in a country like America everyone is allowed an equal opportunity to pursue their dreams, and that’s why you have so many stories of rags to millionaires and billionaires in a country like America versus most countries. So, as much as we might like to say luck doesn’t play a role, many times it does. Sometimes just by the virtue of where you are born, is a massive head start. [Back to Contents] Page 16 Sure, you have to keep putting in the work, and the harder you work, more you increase your chances of getting “lucky.” But to completely rule luck out, where you were born and especially, if there was no equal opportunity, then saying, “luck doesn’t matter”, would not be a fair assessment in my view. Also, if you are lazy and don’t put in the work, you have 0 right to blame luck. So, work to the extent, where you take luck out of the equation. 3: RESILIENCE: Resilience is another trait most entrepreneurs are acutely aware of. However, before I go any further with Resilience, I would like you keep in mind, resilience is often confused with being “Thick Skinned”. Although the two terms can sound same, or at least very similar, there is a vast difference between the two, as you will discover it in the Point 4. Now, let’s get back to understanding and appreciating what Resilience is, and why you need it. We all know, on your journey of entrepreneurship, setbacks are absolute part and parcel of the journey and the process. You pursue your goals for long enough, you will encounter plenty of failures. Simple reason being, journey to top is seldom linear a line. You speak to any successful entrepreneur, and they will tell you 20 stories of the setbacks they suffered, and how they dusted themselves and got back up on the proverbial horse to continue their march towards their goals. It is this characteristic of “Never Say Die” attitude, what resilience is & what is needed, if you want to give yourself the best possible chance of reaching your goals. As I mentioned above, journey to the top is seldom linear or smooth. There will always be hiccups and setbacks. What will separate you from all the “also rans” will be, your WILL and Resilience in never giving up. Thick skin is your ability to take all the negativity and/or slander that might come your way for the choices you make or the vocal opinions you might have. But, if you are clear in your conscience and know you are ethically, morally correct, and you believe in your vision, then I strongly advise, pay them no mind. Many times, people will push you and prod you, only having 1 objective; to get a reaction out of you. And, I can tell you from 1st hand experience, for passionate people it’s not always easy to ignore or let go of unfair criticism. But likewise, you can’t and must not react to everyone who is trying to have a go at you or bring you down. Always remember, the more successful you become, more detractors and naysayers you will draw. And every time you react and give time to these people, it’s taking those precious minutes and your creative energies away from your goals. This is of course not to say, you become a monk and never react. Of course, sometimes you need to and must react, because silence can often be misconstrued as guilt or you are doing something wrong. When the situation truly does warrant, you must react, speak up, take action; then you must. However, if it doesn’t fit the above criteria, and it’s just another troll, who has nothing better to do – then simply scroll past and block the person & this metaphor should also be applied to real life too. Likewise, if you have energy drainers or unfairly negative people in your life, no matter how close they are in relation to you, you must have the courage to severe the ties. People who aren’t aligned with your goals, vision and aren’t supportive, or at the very least stay out of your lane, have no right to stay in your lives either. Some might say, “oh, that’s a bit harsh”. Well, who told you, road to the top was rosy and easy? You will often have to make tough decisions. If you won’t, then you must also make peace with sacrificing your own goals and never fulfilling your true potential You can either be a people pleaser, or you can be ruthless in cutting people who don’t align. However, you can’t be both! 4: THICK SKIN & PICK YOUR BATTLES CAREFULLY: As I briefly mentioned above, Thick Skin and Resilience aren’t exactly the same things, but yes, closely related nonetheless. 5: SELF SWOT ANALYSIS: This is one of my favorites. For those of you who might not be familiar with this term. SWOT stands for Strengths – Weaknesses – Opportunities & Threats. This is something lot of companies undertake periodically to assess where exactly they are in each of the above Alan Aragon’s Research Review – May 2020 [Back to Contents] Page 17 mentioned 4 areas, and then formulate plans to address each of the points to become better prepared to make better decisions. there have been some fundamental changes in any of the 4 columns – for example, a new skill acquisition is a new Strength). You must also add your good and bad habits in there too. One of things lot of young entrepreneurs struggle with when they are starting out is “Clarity”. Especially in today’s day and age, when there is a huge influx of information overload coming at you. Instead of getting a clearer picture on any topic or subject, you end up getting more confused than ever. This can be an exceptionally powerful tool to take your self – awareness from 0 to 100 in a very short space, and is a great tool for continuous improvement in all spheres of your life. Now, Self SWOT Analysis is something I really like for individuals. 6: CRITICAL THINKING – CREATIVE THINKING – INNOVATION - ADAPTABLITY: This is what I believe sets apart the ultra – successful from the rest. Reason being, over the years I have found, many people are just sailing through their lives, often having very little to 0 self – awareness. And, if you aren’t acutely aware of your Strengths and Weaknesses plus all Opportunities and Threats you are surrounded with, then the question you ought to ask yourself is, are you giving yourself the best possible to chance to achieve your true potential? Answer probably would be, no. So, if that’s the case, then why not first identify those, before you embark on your journey, instead of starting something, and being ill prepared. Now, I am not saying, there aren’t individuals who are exceptionally intelligent, have a great deal of natural self – awareness and, are acutely aware of all their Strengths and Weaknesses. However, for majority people, especially the younger age group, it’s a “Conscious” effort of self – discovery. Now, the next obvious question is, how do you go about it. Well, the way to do this is simple. You grab a pen and paper, draw 4 columns and in each column, you write down as many, but relevant attributes. For example, confidence might be 1 of your strengths, however you don’t know when to keep quiet. Likewise, your gym’s size has been your strength, however you know there is a bigger gym coming up near you, and next thing you realize, that new big gym is your threat now. So, now you know, confidence is your great strength, however you need to address the issue of when to keep quiet and master that skill. Likewise, you know till now the size of your gym was your strength, but now that there is a bigger gym coming up, you will need to come up with strategies to combat that threat. The Key to SWOT analysis is, you must do these on regular basis (anything between 3 to 6 months, or whenever you feel you are ready to do it again, because Alan Aragon’s Research Review – May 2020 First, we will address critical thinking. There are numerous definitions and umbrella of what Critical Thinking (CT) actually is; it depends what textbook/ideology/expert you choose to follow. Below is what CT is to me: 1. Forming an opinion/judgement on a presented piece of Information, situation, story, narrative, by analysing it and separating Fact from Fiction. It is to think RATIONALLY & CLEARLY, without letting your Personal Biases, Likes or Dislikes & always keeping the PROVEN FACTS as your central guiding light & never moving away from that. 2. In simpler language, it is your ability to think RATIONALLY & CLEARLY & TO CUT THROUGH ALL THE NOISE. 3. CT is not to think or look at any situation with any prejudices or preconceived ideas/opinions – Always coming back to RATIONALITY & CLARITY – STAYING TRUE TO THE FACTS. 4. CT is not to let the person’s or story’s magnanimity cloud your thinking & subsequently, all important, FINAL JUDGEMENT. 5. CT has no finite time line – Sometimes you might be able to reach the final conclusion & form a judgement within seconds & sometimes, it could take months – It will almost always come down to the COMPLEXITY OF THE MATTER PRESENTED – More complex a matter – more facts and information you need to sift through; the longer the Critical Thinking Process would (& should) last. True essence of CT to me is, to NOT RUSH THE PROCESS & likewise, NOT DRAG IT UNNECESSARILY – Ultimately, you DO [Back to Contents] Page 18 NEED A FINAL JUDGEMENT/OPINION TO MAKE A DECISION. 6. You are presented with 10’s of situations every day, that require CT of some level, and you can’t be a slave to paralysis by analysis – Your ability to do the REQUIRED CT & REACH THE END JUDGEMENT/OPINION/DECISION WILL ULTIMATELY IMPACT – HOW “EFFICIENT” YOU ARE. Many people will spend far too long on trivial matters, and will then try to justify by saying, “oh, it needed to be thought through carefully”. For example, you go to buy disposable paper plates, and if you spend half hour trying to choose which colour to buy, then that is not CT. As it does not fit the basic criteria of CT – RATIONALITY & CLARITY – Does choosing the colour of paper plate require half hour, when it’s something you are just going to use once and throw away. That’s not CT. That’s just a sheer waste of VALUABLE TIME. 7. CT is not meant to hinder/confused with CREATIVE THINKING – Critical Thinking is about SEPARATING FACT FROM BULLSHIT IN THE MOST EFFICIENT AND TIME SAVING MANNER, WITHOUT LETTING ANY PERSONAL BIASES, PREJUDICES AND PRECONCEIVED OPINIONS – PERIOD. Now let’s move onto Creative & Innovative Thinking + Adaptability – All these 3 come under Critical Thinking Umbrella to me. However, some people might want to put these 3 in independent classes of their own, and that’s fine too. Neither diminishes their worth or importance. One of things that sets super successful people is, not only they possess the basic universal qualities of Hard Work, Resilience, Thick Skin, but they also are incredibly Creative, Innovative and Opportunistic (used here in a positive tone) and often have a unique gift of “foresight,” as well as being Adaptive to any sudden situation. Most ultra-successful people are incredibly intuitive in seeing trends, and thus planning and taking ACTION before the crisis hits, or find opportunities during crisis. Classic Case Study could be shutting down of tons of book stores when Amazon started creating it’s foothold in the books segment. The companies which didn’t take notice of the impending and unavoidable advances in technology, eventually went out of business. very big on gourmet coffee and Australia has a huge breakfast culture. So, staying “ahead” of the curve and applying Creative & Innovative Thinking, they had the wherewithal and the foresight to launch their 1st McCafe style restaurant way back in 1993, and since then it’s taken a life of its own. This is a classic example of executing Creative & Innovative Thinking, and not being tied by certain set philosophies. Many businesses and entrepreneurs confuse the need to evolve with changing times with as if they are selling their soul to the devil. ADAPTIING to changing times “ethically” is not selling your soul to the devil, it’s called having the courage and foresight to ACCEPT the often inevitable. We are living in incredibly unpredictable and unprecedented times. Things are changing faster than ever, and the world we have come to know over our existence, might never be the same. For example, if you have always done 1 on 1 PT, and you never looked into Online Coaching, now might be the time to seriously look into it. This is being “Responsive/Reactive” to change, which is already upon you! The most successful ones are generally “Proactive”, and often have the wherewithal to be prepared well in advance, before the actual change arrives. So as an entrepreneur, if you don’t quickly develop these skills, you might find yourself in real big trouble – as lot of businesses just found out! 7: PR & SALES SKILLS & ABILITY TO READ PEOPLE: This is something many people not only underplay, but actually many times don’t pay any attention at all. And, I think it’s one of the biggest mistakes any entrepreneur can make. People often ask me what makes a great Fitness Professional/PT/Coach, and whilst there is no 1 concrete definition. To me a great Fitness Professional is someone: 1: Who can deliver amazing results in the shortest possible time and safely. 2: Is evidence based. 3: Is ethical 4: Makes ton of money. On the other hand, let’s take example of McDonalds Australia. McDonalds think tank identified, Australians are Now, I’ll accept, many might not have the 4th as their goal, and that’s fine. However, if 4th IS also 1 of your objectives/goals, then unless you develop amazing Public Relation Skills & Sales Skills, you will struggle to fulfil your true potential or do justice to your amazing knowledge. Alan Aragon’s Research Review – May 2020 [Back to Contents] Page 19 Likewise, your ability to read people is equally important. This is not just about reading every person your meet, and how much of your time and/or energy he/she deserves. It goes much further. It goes into your hiring of staff, doing collaborations, forming business partnerships/alliances, so on and so forth. Last thing you want to do is hire the wrong staff, spend 3 months training them, only to discover they are not the right fit for your business. work or are working, record the duration you are actually productive, and do this diligently on daily basis for few days, and you will very quickly discover, your 15 hour work days, actually only have 5 real Productive Hours, rest are just being wasted or definitely aren’t anywhere near optimal productivity, and once you have this knowledge, you can take steps to increase your productivity. So, no matter what field or industry you are in, unless you master these skills, you will always struggle to do justice to your other skill sets. Often it is better to spend lesser hours “working”, especially when you are not being truly productive in all of those supposed “working hours” and instead focus on being more “productive” for every hour you put in. 8: TIME MANAGEMENT: This is might seem an obvious one, but I think this one is particularly pertinent to the Fitness Industry, especially the Fit Pros who are very active on Social Media. How you choose to use your time and how efficiently you exercise those choices, will often be the difference in your productivity, and ultimately, it will often determine the level of success you attain! Let me share a little something. The 2 of the greatest facts pertaining to humans are: 1: Death – It’s inevitable – at least, till now. 2: TIME – We all have “FINITE” amount of Time on this earth – Every moment that is passed, is NEVER COMING BACK – As well as, each day only has 24 Hours – That is a FINITE period too. Formula to success is unfortunately not easy to condense, and thousand page books have been written on this subject, I still hope you get something out of this piece, that can add a positive addition to your day to day life, and ultimately in your dream chasing. So, it’s up to us, how we CHOOSE to spend each day. Harry Sandhu Team Boss _______________________________________________ I see so many Fit Pros engaging in unnecessary fights on the social media, when they could be utilizing that time to drive their business forward or be spending quality time with their loved ones. Now, we all have been guilty of this – me as well, just as much as anyone else. However, not being “aware” of it and not trying to improve utilization of time you have is a huge disservice to your goals. All the best in your endeavors. Harry is a Contest Prep Coach, who holds an Under Grad in Exercise & Sports Science, with Major in Sports Nutrition from University of Deakin, Melbourne. Australia. Editor’s note: more information can be found at Harry’s website, teambossfitnessacademy.com Likewise, so many people are spending one of the most valuable commodities available to humans, on absolutely mindless things which add no short, or long term value. I would like to share a simple strategy to “PRODUCTIVITY”, because ultimately, many hours you spend “thinking” you are key is, out of those hours, how many hours truly productive. improve your it’s not how working. The are you being You might think, you just spent 2 hours on the laptop working, but out of those 2 hours, if you spent 1 hour just trolling the internet, the question you must ask yourself is, “were you truly productive for those entire 2 hours, or you just ticked a box?”. To address this, what I recommend is to have a timer on your desk, or wear a watch. Each time you sit down to Alan Aragon’s Research Review – May 2020 [Back to Contents] Page 20 How can we track body composition changes with minimal technology and resources? By Alan Aragon _______________________________________________ I’m frequently asked what’s the “best” way to measure body composition. The technical answer is best quoted from the ISSN position stand on diets & body composition,1in an excerpt from the summary points which I vividly remember authoring: “All body composition assessment methods have strengths and limitations. Thus, the selection of the method should weigh practicality and consistency with the prohibitive potential of cost, invasiveness, availability, reproducibility, and technician skill requirements. Ultimately, the needs of the client, patient, or research question should be matched with the chosen method; individualization and environmental considerations are essential.” Given the above, it’s possible to track progress with lowtech, economical methods as effectively as it is with hightech, expensive instrumentation. All methods have pros and cons. Lately, I’ve received a lot of questions about how to track body composition with minimal resources and technology. My answer is as simple as weight, waist, and strength. The technology to assess bodyweight, waist girth, and strength requires no special purchases since most people already own a bodyweight scale and measuring tape. A gym membership or home gym would be the biggest barrier to strength training, but with gyms reopening gradually, and home gym building becoming more popular, this barrier is not necessarily a deal breaker. A multitude of bodyweight scales are available. They are all pretty straight-forward in their function. If you’re somewhat OCD, you can buy two of them to see how they both track, since it takes only seconds to weigh yourself. Weighing should be done daily, first thing in the morning, after voiding your bladder. A weekly average (7 days of data divided by 7) will give you a more accurate picture of progress, since weight can fluctuate up and down sometimes on a day-by-day basis. It’s the longer-term trend that counts. Measuring waist girth is a matter of being consistent with how and where on your body it’s done. The objective is to standardize the protocol so you can avoid potential confounders such as variance in lung content, gastrointestinal content, and even muscular contraction vs relaxation level. Once again, this measurement should be taken first thing in the morning after voiding the bladder. After that, blow out as much air as you can, then tighten your core (or you can think of tightening your “abs’ while remaining upright). This standardizes the relative contraction level of measurement, since it’s possible to introduce significant variance if you “pooch” your stomach out compared to tightening it up. The objective is to tighten it up as much as possible, and do the same thing each time you take the measurement. It’s important to keep the location of the measurement consistent each time. I use the very convenient landmark of the navel, and don’t slant the tape up or down when you take the measurement; keep the tape parallel to the floor. Measure waist girth weekly, take a monthly average. If you want to measure this daily and take a weekly average, I’m not gonna stop you, but note that the psychological “weight” of such small changes can be fatiguing to track; it’s like watching paint dry or grass grow. I was in private counseling practice for at least a decade. I had my own office space where I took body comp assessments using the scale and calipers. I preferred calipers because I enjoyed the autonomy and control of choosing the exact subcutaneous locations to track, and I had enough experience to be very consistent with the method. However, when I shifted my practice to an online model, I had clients use the scale, tape, and strength as a body composition progress gauge. It worked like a charm. Let’s talk about each component. Tracking strength changes is a good ballpark indicator of changes in lean mass. If you’re trending upward in strength, size gains will not be far behind. If you’re trending down in strength, lean mass losses will follow. Note that these changes are specific to the muscles affected by the exercises you’re tracking. In other words, if you’re trending up in bicep curl strength, you’re not automatically going to gain size in the quads, unless of course strength is trending up in quad training as well. Alan Aragon’s Research Review – May 2020 [Back to Contents] Page 21 Tracking strength which is most reflective of lean mass flux would be the performance of multiple repetitions. Single-repetition max testing is not necessary, nor is it as accurate a reflection of potential changes in muscular size due to its heavy reliance on neurological facilitation. What better reflects increases in muscle mass are upward trends in the amount of weight you can use in approximately the 6-12 rep range (5-10 rep range can work for this as well). This does not mean that all other rep ranges are off the table to use as proxies for presuming the loss or gain of lean mass. If you’d rather track your strength changes in the 10-15 rep range, that can work as well. Let me be clear that muscular size gains are possible from training in a wide repetition range.2 In my observations, tracking strength in rep ranges much higher than 15 or much lower than 5 do not provide the best surrogates for reflecting changes in muscular size. I would also add that you don’t have to track the strength gains for every single lift – only the lifts involving the muscles you care most about either growing or maintaining the size of. And note that its doesn’t have to track all of the exercises you do for those muscles. Just track the strength trend of one of the exercises per muscle group you’d like to keep an eye on. Strength changes are best judged on a monthly basis. Keep in mind are that daily changes tell you next to nothing. Weekly changes give you hints at possible trends. Monthly differences tell the real story of what direction you’re headed. Adding an extra wrinkle here, you may choose to take monthly pictures to keep a visual log of progress in addition to weight, waist, & strength. The best way to take progress pics is to wear the same clothing (underwear or bathing suit), same lighting, same location, and same posture. Take a minimum of front & back view. Take a side view shot if you specifically want a visual tracking of changes in the silhouette of your glutes and/or abdomen. If you want to use the tape to track limb and/or chest and/or hip circumference in addition to your waist circumference, be my guest. Just remember that waist circumference is the girth measurement most closely reflective of general changes in fat mass. Ant there you have it: low-tech, high-value progress tracking that doesn’t require a specific facility or high degree of technical expertise. Good news for online practitioners or coaches – you don’t have to be physically there to help your clients track their body composition progress. References: With the finer points of weight, waist, & strength out of the way, here’s how to interpret your data: 1. Aragon AA, Schoenfeld BJ, Wildman R, et al. International society of sports nutrition position stand: diets and body composition. J Int Soc Sports Nutr. 2017;14:16. Published 2017 Jun 14. [PubMed] 2. Schoenfeld BJ, Grgic J, Ogborn D, Krieger JW. Strength and Hypertrophy Adaptations Between Lowvs. High-Load Resistance Training: A Systematic Review and Meta-analysis. J Strength Cond Res. 2017;31(12):3508‐3523. [PubMed] Alan Aragon’s Research Review – May 2020 [Back to Contents] Page 22 “You have within you right now, everything you need to deal with whatever the world can throw at you.” – Brian Tracy If you have any questions, comments, suggestions, bones of contention, cheers, jeers, guest articles you’d like to submit for consideration, send it over to support@alanaragon.com. Alan Aragon’s Research Review – May 2020 [Back to Contents] Page 23