Biosorption of diesel in ocean contaminated
water using Oregano (Origanum vulgare)
A Scientific Write-Up Presented to the Faculty
Of Landy National High School
In Partial Fulfillment of the Requirements
for Research 1
Precious V. Lazo
Glycel Ann P. Reynoso
Ashley Conce P. Sosa
Researchers
Kamillahclare Fernandez
Cyrus Vincent Paradero
Student Adviser
Paolo Andrew M. Pantoja
Adviser
Table Of Contents
Acknowledgement
Abstract
Research Plan
Introduction………………………………………………………………………… 1
Statement of the Problem……………………………………………………………2
Objectives of the Study……………………………………………………………...2
Significance of the Study
2
Scope and Delimitations…………………………………………………………….2
Review of Related Literature
Definition of Terms
3
3- 4
Methodology
5
Research Design
5
Research Locale
5
Materials and Equipment
5
Procedures
5- 6
Data Gathering
6
Flow Chart
7
Table Of Contents
Abstract
Acknowledgement
Research Plan
Introduction………………………………………………………………………… 1
Statement of the Problem……………………………………………………………2
Objectives of the Study……………………………………………………………...2
Significance of the Study
2
Scope and Delimitations…………………………………………………………….2
Review of Related Literature
Definition of Terms
3
3- 4
Methodology
5
Research Design
5
Research Locale
5
Materials and Equipment
5
Procedures
Data Gathering
Flow Chart
Research Plan
A. Problem Being Addressed
5- 6
6
7
The main problem to be answered by the study is
Why oregano can absorb diesel in ocean contaminated water?
The study also sought to answer the following questions
1. How many oregano can clean water contaminated with diesel?
2. How long will it take to absorb the diesel?
B. Goals/ Expected Outcomes/ Hypothesis
Objective of the Study:
Main Objective:
To determine if Oregano can absorb diesel in ocean contaminated water.
Specific Objective:
To determine how long will it take to absorb the diesel.
To identify the components of oregano that will absorb the diesel; out of
the ocean contaminated water.
Hypothesis
Oregano has components that will absorb the diesel out of ocean contaminated
water.
C. Research Instruments
In the process the researchers used are seawater, diesel, oregano, container and
measuring tools.
D. General Procedures
A. Gathering of Materials:
The researchers gathered materials from their locality. They gathered 150 ml
of seawater, 0.66 of diesel, and oregano. They also gathered a container and
measuring tools.
B.Preparing the Materials and Equipment:
The researchers sterilized all the equipment to ensure that there is no
microorganisms that will affect the result.
C.Contaminating the seawater
Put the 150 ml of seawater in a container. Then contaminate it by putting 0.66 ml
of diesel.
D.Putting the oregano on top of the seawater
Put the oregano on top of the contaminated water.
E. Observe
Observe if the diesel was absorbed by the oregano.
Chapter 1
The Problem and Its Background
Nowadays, water pollution is becoming a bigger issue. This problem exists all
over the world. Water pollution ends up causing harmful side effects on the inhabita-
nts in the area that must survive of this water. Many people, including us, regard
pollution as a problem that is hard to solve, instead it will get worse and worse as time
passes by.
Recently our government was tasked to take care of this problem to protect the
precious drinking water and seawater, most corporation dump harmful waste near
water supplies.
At certain times pollution is caused by the on purpose like waste water from oil
refineries or accidents also account for water pollution. Water contaminated with oil
is a global plague that affects both plants and humans and also for us humans, not just
for drinking. It is one of the main sources of food for example is fish.
So we decided to use Oregano (Oreganum vulgare) to clean the water by
absorbing diesel because it is chemically modified with HNO3 and H3PO4 used as
adsorbent for removal of both acids in water thus few of herbaceous plants like this is
a bio sorbent.
1
Statement of the problem
1. What is the components of Oregano that will absorb the diesel out of the ocean
contaminated water?
2. How many amount of oregano can absorb the diesel?
3. How long will it take to absorb the diesel?
Objectives of the Study
Main Objective
To determine if oregano can absorb diesel in ocean contaminated water.
Specific Objectives
To determine how long will it take to absorb the diesel.
To identify the components of oregano in absorbing diesel in ocean
contaminated water.
Significance of the Study
This study can clean water contaminated with diesel.
To the community our water bodies, the animals and plants that live in it will be
clean and safe. It is important to us for water bodies like rivers and seas are one of the
main sources of our food for example is fish.
To the future researchers it will be a great help for them to have an advanced
knowledge about a study related to this.
Scope and Delimitations
This study focuses on cleaning ocean contaminated with diesel using Oregano
(Origanum vulgare).
Hypothesis
The researchers conclude that this study has a posibility to absorb diesel in
ocean contaminated water using Oregano (Origanum vulgare).
2
Chapter 3
Methodology
Research Design
The researchers used an experimental type of design because the research
manipulates variables and they’ve conducted different trials. Another type of design
used is sequential type of design because you can’t proceed to the other step if you
didn’t accomplish the first step.
Research Locale
The experiment was conducted at Barangay Landy Santa Cruz Marinduque as
well as the materials gathered.
Materials and Equipments used
The materials gathered and used in the study include the following.
The materials needed are 15 ml of seawater, 1/8 teaspoon of diesel, oregano,
measuring tools and a container.
Procedures
A. Preparation of materials
First the researchers gathered all the materials that are needed which are
seawater, diesel, oregano, measuring tools and a container.
B. Preparing the set up
First put 150 ml of seawater in the container, and then contaminate it by
putting 0.66 ml of diesel. Next put some oregano on top of the seawater that is
contaminated and investigate if it absorbed the diesel that contaminates the water.
Data Gathering
The researchers want to use oregano as a bio sorbent to clean ocean
contaminated with diesel because it is chemically modified with HPO3 and H3PO4
used as adsorbent for removal for both acids in water thus herbaceous plants like these
are bio sorbent.
Flow Chart
Collecting of the needed
materials
Preparing the experiment set- up
Abstract
This investigatory project deals on the preparation and acceptability of
Biosorption of diesel in ocean contaminated water using Oregano (Origanum
vulgare).
This study used fresh oregano leaves as an ingredients/ materials in processing
Biosorption of diesel in ocean contaminated water using Oregano (Origanum vulgare)
that would clean our ocean.
Experimental method was employed in this project. The research was conducted
at Barangay Landy Santa Cruz Marinduque within the month of November-
December. Biosorption of diesel in ocean contaminated water using Oregano
(Origanum vulgare) focused on the ratio of diesel and oregano.
A modified material was used and its level of acceptability was evaluate.
The results were tabulated and analyzed. Results of the study revealed that
oregano leaves is feasible in Biosorption of diesel in ocean contaminated water using
Oregano (Origanum vulgare).
Based from the results of the study and other benefits the researchers concluded
that oregano leaves can be used in Biosorption of diesel in ocean contaminated water
using Oregano (Origanum vulgare).
It is recommended that follow up studies for further enhance mentor improvement
of the study be done.
Acknowledgement
The researchers would like to precise depth gratitude to the people who help
successfully to conduct this research. The following helping hands in one way or
another had lent their time in the completion of this research;
First of all, the researchers would like to express their thanks to the Heavenly
father, who gave them strength and intelligence to overcome all the challenges in this
study.
To Mr. Lino Peñaredonda, who gave his full support to finish this study.
To their adviser Mr. Paolo Andrew M. Pantoja, who advice them how to conduct
a study, sharing his time, answer to the researchers question and to gave other
information.
To their friends and classmates, who help in some problems to finish this study.
To their students adviser, who checked their papers before it accept by their
adviser Mr. Paolo Andrew Pantoja.
To all teachers of Landy National High School, who also give their full support in
accomplishing this research.
And to their lovingly parents, who gave them a moral support and gave an
inspiration as a lovingly parents.
This study would not be successful without their encouragement and help. Thanks
to all of you.
-Researchers