Microbiology Select the alternative that correctly answers the following questions. 1. A fungus would belong to the phylum (a) (b) (c) (d) 2. How do viruses reproduce? (a) (b) (c) (d) 3. by binary fission by sexually reproducing by replication in living cells by budding Which type of microrganisms is unable to be killed by antibiotics? (a) (b) (c) (d) 4. Phaeophyta Bryophyta Mycophyta Cyanophyta bacteria viruses fungi protists The diagram above is an example of which type of organism? (a) (b) (c) (d) fungal spore bacterium virus paramecium Microbiology 37 5. What method of reproduction do bacteria use? (a) (b) (c) (d) 6. Which of the following diseases is caused by a fungi? (a) (b) (c) (d) 7. malaria cholera ringworm measles Bacteria form spores in unfavourable conditions. These spores can then reproduce when the conditions change. Which of the following lists a way in which bacterial spores can be killed? (a) (b) (c) (d) 8. budding spore formation binary fission meiosis using antiseptic boiling for more than 10 minutes drying them out cutting them open A dish of sterile nutrient agar was exposed to air for a short time and was then covered and incubated for 14 days. At the end of this time it was noted that there were two types of growth present. There were large patches of a white furry colony and some small yellowish, orange colonies. What type of organism would the furry white growths represent? (a) (b) (c) (d) 9. bacterial colony mould colony protozoan colony fungal colony Which of the following organisms is only visible using an electron microscope? (a) (b) (c) (d) bacteria yeast cells viruses fungi Microbiology 38 10. Fungi are unable to make their own food and are called saprophytes. Which chemical do they lack? (a) (b) (c) (d) 11. The diagram above is an example of (a) (b) (c) (d) 12. virus rickettsia streptococcus spirochaetes Some examples of fungi are (a) (b) (c) (d) 13. lipase chloroplast lysosome chlorophyll bacilli and yeast cocci and tinea mould and yeast mould and protista Viruses cause many diseases in humans, most of which are unable to be treated effectively. Which of the following diseases is caused by a viral organism? (a) (b) (c) (d) dysentery chicken pox tuberculosis athletes foot Microbiology 39 The following information is relevant to questions 14 to 16. A biologist was investigating whether certain liquids contain agents which cause decay. The following table sets out details of the experiment in which 5 test tubes of clear nutrient broth was prepared inoculated and then sealed. The tubes were checked at 24 hours and 48 hours. Tube Nutrient medium Inoculum Appearance after 24 hours 48 hours 1 100ml sterile broth 100mL sterile broth 100mL sterile broth 100mL sterile broth 100mL unheated broth 1mL sour wine Cloudy cloudy 1mL tap water Clear cloudy 1mL boiled sour wine 1mL distilled water No inoculum used Clear clear Clear clear Cloudy cloudy 2 3 4 5 14. The cloudy appearance which developed in some of the tubes most probably resulted from (a) (b) (c) (d) 15. The tube which is of least value in the experimental design is tube (a) (b) (c) (d) 16. 1 2 4 5 Which of the following interpretations of the data is best supported by the evidence? (a) (b) (c) (d) 17. effects of the growth of microorganisms the development of a chemical reaction between the nutrient broth and the inoculum the temperature changes to which the broth was subjected a putrid odour tap water contains vast numbers of bacteria boiling destroys bacteria bacteria are unable to reproduce in sterile broth bacteria present in distilled water are unable to reproduce in broth Which of the following is true for all viruses? (a) (b) (c) (d) viruses are bigger than bacteria viruses cause disease in humans viruses can only reproduce in a living cell viruses form resistant spores Microbiology 40 18. Amoebae belong to the class (a) (b) (c) (d) 19. The fingerlike processes that flow out from an amoeba as it moves are called (a) (b) (c) (d) 20. flagellate amoeba paramecium slime mould Archaebacteria and Eubacteria belong to the kingdom (a) (b) (c) (d) 22. contractile vacuoles flagella pseudopodia cilia The organism shown above is a/n (a) (b) (c) (d) 21. Ciliata Sarcodena Sporoza Flagellata Animalia Plantae Protista Monera Another term for rod shaped bacteria is (a) (b) (c) (d) spirilla bacilli cocci ovoid Microbiology 41 23. Many monera are motile and so can move about actively. They do this by means of a (a) (b) (c) (d) 24. The virus represented by the diagram above is a virus known as a (a) (b) (c) (d) 25. retrovirus bacteriophage polymerase viroid The bacteria used to produce yoghurt do so by consuming some of the milk to produce (a) (b) (c) (d) 26. microtubule flagellum capsule phagocyte hydrochloric acid lactic acid carbon dioxide methane Recombinant DNA technology makes use of (a) (b) (c) (d) endonucleases plasmids viroids retroviruses Microbiology 42 27. The lifecycle drawn above is representative of (a) (b) (c) (d) 28. a fungus a true slime mould a cellular slime mould euglena Some Biologists classify the bacteria ,rickettsias and the blue-green algae together in the kingdom Procaryota. One feature they all have in common is that they (a) (b) (c) (d) reproduce by spores possess chlorophyll are parasitic have no distinct nucleus Microbiology 43 29. In the diagram above the structure labelled X is a (a) (b) (c) (d) 30. A germ killing substance produced by a bacterium or mould is a/n (a) (b) (c) (d) 31. toxin antibiotic antibody toxoid Bacteriophages are (a) (b) (c) (d) 32. hyphae sporangium rhizoid stolon viruses which attack bacteria bacteria which attack other bacteria bacteria which attack viruses viruses which attack viruses All bacteria have in common the fact that they (a) (b) (c) (d) are parasites reproduce only in moist surroundings are the same shape are able to form spores Microbiology 44 33. One important property of cyanobacteria is that they (a) (b) (c) (d) possess chlorophyll c are obligate anaerobes ferment fix atmospheric nitrogen Microbiology 45