Q1. DNA polymerase synthesizes:
a. DNA in 5’-3’ direction
b. DNA in 3’-5’ direction
c. mRNA in 3’-5’ direction
d. RNA in 5’-3’ direction
Answer: a
Q2. The mode of DNA replication is:
a. Conservative and bidirectional
b. Semiconservative and unidirectional
c. Semiconservative and bidirectional
d. Conservative and unidirectional
Answer: c
Q3. Which enzyme is used to join nicks in the DNA strand?
a. Primase
b. DNA polymerase
c. DNA ligase
d. Endonuclease
Answer: c
Q4. The synthesis of DNA on the lagging strand, takes place in segments, these are called:
a. satellite
b. double helix
c. kornberg segments
d. Okazaki fragments
Answer: d
Q5. Short strands of _____ are used to make the primers of DNA replication.
a. DNA
b. RNA
c. Histone
d. Protein
Answer: b
Q6. Which of the following helps to keep the ssDNA strand stable?
a. DNA polymerase II
b. Binding proteins
c. DNA polymerase III
d. helicase
Answer: b
Q7. Semi-conservative replication means:
a. parts of the old strand is joined to parts of a new strand
b. Each of the original DNA strands serves as a template, but none of which remain inside the nucleus
c. both old and new strand stays inside the parent cell, both replicated strands go to the daughter cell
d. One old strand and one new strand stays inside the parent cell, one old strand and one new strand goes to
the daughter cell
Answer: d
Q8. X and Y are replication directional arrows, and A, B and C are regions on the DNA molecule. The origin of
replication would be located at:
a. A
b. B
c. C & A
d. X & Y only
e. Y
Answer: b
Q9. Which enzyme binds only to dsDNA?
a. topoisomerase
b. helicase
c. binding proteins
d. ligase
Answer: a
Q10. During a lab experiment, a student is trying to construct a new single strand of ssDNA from the original
‘single strand’ ssDNA. She places all the following into a micropipette. Which is an ingredient she doesn’t need?
a. DNA polymerase II and III
b. ligase
c. primers
c. helicase
d. nucleotides (dNTPS)
Answer: c
Q11. The enzyme that splits open the DNA creates what's called a fork - where the DNA separates into the
lagging strand and the ______ strand.
a. leading
b. lagging
c. okazaki
d. fragmented
Answer: a
Q11. Which of the following help to keep the dsDNA strand stable?
a. DNA polymerase II
b. Single strand binding proteins
c. topoisomerase
d. helicase
e. DNA polymerase III
Answer: c
Q12. In what phase of the cell cycle does DNA replication take place?
a. mitosis
b. G2
c. synthesis
d. G1
Answer: c
Q13. In a PCR experiment, a particular set of bases in the template strand of DNA is AGTTGTAA. Which of the
following is the corresponding pairing DNA primer sequence?
a. TCAACATT in the 5' to 3'direction
b. TCAACATT in the 3' to 3'direction
c. TCAACATT in any direction
d. UCAACAUU in the 5' to 3'direction
Answer: a
Q14. What is the open area between the replication forks called?
a. X and Y
b. Replication bubble
c. Replication circle
d. Zone of replication
Answer: b