Lebanese University – Faculty of Pedagogy – Deanery Practicum 2-Microteaching Presented to : Dr.Nadeen Fayyad Prepared by : Nasima Miah (ID: 5922) After several weeks How the body is able to repair these wounds ? MATERIALS Try to construct a model of what is occurring in each phase of mitosis (using the card descriptions) using the pipe cleaners, beads, ping pong ball with yarn attached, and large ring. Prophase: -Nuclear membrane disappears -Centrosome duplicates and becomes 2 asters -Achromatic spindle fiber appears -Chromosome consist of 2 chromatids joined by a centromere. Metaphase: -Spindle fibers from opposite ends of the cell pull on chromosomes -Chromosomes are aligned along the middle of the cell. Anaphase: -Spindle fibers shorten and pull sister chromatids to opposite ends of the cell Telophase: -Nuclear membrane re appear -Aster becomes centrosome - Achromatic spindle fiber disappear -Formation of 2 daughter cells having each chromosome consist of 1 chromatid MITOSIS Mitosis is the process of cell division which occurs on somatic cells. It produces identical copies of cells, where the mother cell divides to produce two new cells the daughters that are genetically identical to itself Mitosis consists of four phases : prophase , metaphase , anaphase, and telophase. INTERPHASE Interphase is important for cell division because it allows the cell to grow, replicate its DNA, and make final preparations for cell division Chromosomes are not clearly discerned in the nucleus. PROPHASE -Chromatin in the nucleus begins to condense and becomes visible in the light microscope as chromosomes. -Nuclear membrane disappears -Centrosome duplicates and becomes 2 asters -Achromatic spindle fiber appears -Chromosome consist of 2 chromatids joined by a centromere In a normal human 2n = 46 chromosomes But for simplification we will use 2n = 4 chromosomes METAPHASE Spindle fibers align the chromosomes along the middle of the cell nucleus. This line is referred to as the metaphase plate ( equatorial plate) ANAPHASE Centromere divides and Sister chromatids separate and move to opposite sides of the cell forming (Polar Ascension). TELOPHASE Nuclear membrane re appear -Aster becomes centrosome - Achromatic spindle fiber disappear -Formation of 2 daughter cells with 2n chromosomes ,each chromosome consist of 1 chromatid -cytokinesis or the partitioning of the cell may also begin during this stage. CYTOKINESIS Cytokinesis is the physical process of cell division, which divides the cytoplasm of a parental cell into two daughter cells. In cells such as animal cells, this fissure, or “crack,” is called the cleavage furrow. In plant cells, this structure is called a cell plate. IMPORTANCE OF MITOSIS: It ensures: • the build up and growth of the organism • its cellular renewal • the maintenance of its biological identity. SLIDES UNDER THE MICROSCOPE Slide 3 : Slide 1 : Slide 5 : Slide 2 : Slide 4 : ANSWERS : SLIDES UNDER THE MICROSCOPE Slide 3 : prophase Slide 1 : metaphase Slide 5 : Telophase Slide 2 : anaphase Slide 4 : cytokinesis Name: ________________________________ Date: _________________________________ Duration: 10 minutes Match column A with column B Column A Column B 1_Anaphase a. The nuclear membrane begins to disassemble 2_Telophase b. Chromosomes are aligned along the middle of the cell 3_Metaphase c. Spindle fibers shorten and pull sister chromatids to opposite ends of the cell 4_Prophase d. Cell membrane pinches in to completely surround each new daughter cell. 5_Cytokinesis e. Nuclear membrane forms around each set of chromosomes. Good work!