Cell cycle analysis
In Eukaryotes, the cell cycle is composed by four phases: G1 phase (growing), S phase (synthesis), G2 phase
(growing) and M phase (growing). The state of a quiescent cell is called G0 phase.
The percentage of cells treated with higher Curcumin concentration in the peak of G0/G1 should increase,
while cells in the peak of G2 should decrease, in a directly and inversely proportion respectively.
The Curcumin antimitotic activity usually causes an accumulation of cells in the G0/G1 phase because it
inhibits the cell proliferation cascade signaling, upstream to growth factor action, that allows the cells to
move from G0/G1 to S phase. So the cells are blocked in the G0/G1 phase and cannot complete the cell cycle.
Control
No FBS
Curcumin 5 mM