Cytoskeleton Questions (9/10): Centrioles are made of (9) microtubules - Microtubules are what make up cilia and flagella; used for motility o Cilia unique to protists & eukaryotes o Flagella are whip-like appendages that are responsible for prokaryotic cell motility o Pili used to transfer DNA between bacteria during conjugation; no motile function - Microtubules form the spindle that separates homologous chromosomes Intermediate filaments are structural proteins; provide resistance to mechanical stress and compression forces Microfilaments are used for movement and contain the actin filaments used in muscle contraction - Microfilaments are linked with cytokinesis - Microfilaments are used for cell movement, such as with phagocytosis - Microfilaments make up part of cell’s cytoskeleton Exocytosis: releasing of substances, contained in vesicles, to the outside of the cell Pinocytosis: refers to the ingestion of fluids (aka cell drinking) Phagocytosis: engulfing movement to destroy a pathogen; microfilaments are responsible for generating the momentum necessary for this process Villi: macroscopic folding of tissue Microvilli: folding of cell membrane that increase surface area of a cell Epithelial tissue covers both internal and external surfaces of the body Endothelial tissue covers only the internal surfaces of components of circulatory system Gram + is purple and has a thick (peptidoglycan/lipoteichoic) wall - Gram + will hold on to first stain, staining organism purple - Gram + cell envelope thin Gram – is pink and has a thin cell wall, but more layered cell envelope & additional (lipopolysaccharide aka LPS) outer membrane - Gram negative bacteria are considered to be more dangerous because it has additional layer in cell envelope and LPS outer membrane that is an endotoxin & damages hosts Transformation is when bacteria gets genetic information from the environment (Griffith experiment with mice; R strain safe, S strain deadly) - Also known as ‘competence’ Transduction is the transfer of genetic material using a bacteriophage - Lytic phage = causes bacterial death - Lysogenic phage = does not cause bacterial death Conjugation is the transfer of genetic material via a conjugation bridge via a pilus/pili Transposons are DNA sequences that can change their position within a genome; this sometimes creates or reverses mutations