Name _____________________ Honors Biology Date _____ Period _____ Cell Cycle Laboratory Investigation Objectives: What are stages of a cell’s life cycle and what are the events that take place in each of these stages? Vocabulary: (to be used in the conclusion) sister chromatids cell cycle cytokinesis daughter cells nucleus diploid cell plate spindle fiber parent cell nuclear membrane homologous chromosomes cleavage furrow centromere chromatin replicated Procedure: 1. You and your lab partners should use the prepared slides to find and examine cells in the 5 major stages of the Cell Cycle (IPMAT). 2. Draw cells in each of the stages. Label the drawing with the appropriate stage and magnification. Label the following as it applies to your drawing: nuclear membrane, chromosomes, sister chromatids, chromatin, cell plate, cell wall, cell membrane 3. After you find and draw a well-labeled diagram of each stage, get your instructor’s initials. 4. Complete the questions associated with each of the cell stages. You should answer most of the questions associated with the stage at the time you find and draw it. Some of the questions may require you to use reference material. **You should have 5 drawings completed at the end of the lab** QUESTIONS Interphase: 1. Describe the contents of the cell during interphase. 2. Are the nucleolus and nuclear membrane present in the cell? 3. Are distinct rod shaped chromosomes easily visible this time? Answer 4-6 from notes and text 4. 5. 6. Where are the chromosomes located? Why are we unable to see them? What term is used to apply to the nuclear contents during interphase? List the important events that occur in interphase: Prophase: 7. Are chromosomes now visible? What do they look like? 8. What changes occurred to the nucleolus and the nuclear membrane? Answer question 9 from your text and notes 9. Explain why chromosomes are now visible but were not visible during interphase. Metaphase: 10. Where are the chromosomes now located in the cell? 11. What is the evidence of chromosome replication? Explain. Answer questions 12-13 from your notes and text 12. 13. What are the fibers called that become visible during this stage? What is their purpose? What term is used to describe the structure at which each fiber attaches to a chromosome? Anaphase: 14. What occurs to each pair of sister chromatids during anaphase? 15. Toward what area of the cell are the chromosomes being directed? Answer question 16 from your notes and text 16. Why is it necessary for the sister chromatids to move during anaphase? Telophase: 17. What cellular parts begin to reappear during telophase? 18. How many daughter cells have been formed from the original mother cell? 19. Compare the number of chromosomes and the amount and type of DNA in the mother cell and the resulting daughter cells: Additional Question: 20. Why do cells undergo Mitosis? 21. What are the changes that take place with chromatin during the cell cycle?