Male Reproductive System IT IS PRESENTED BY : TAGHREAD BARAK ALTHUBYANI UNDER THE SUPERVISION OF DR.: KHADIJA ABDUL -JALIL FADL AL-DIN • The male reproductive system consists of the testes suspended in the scrotal sacs,, genital ducts, accessory glands, and penis • Testes produce sperm and testosterone • The genital ducts and accessory glands produce secretions required for sperm activity • The secretions from the penile urethra to provide nutrients for sperms Testis • Testis is CYTOGENOUS GLANDS(secretes living cells) , Mixed gland (an exocrine and an endocrine function) • Generally ,three layers covered the testis which is tunica vaginalis, tunica albuginea, and tunica vasculosa. • Tunica vaginalis is a sac of the peritoneum, it surrounds the testis but incompletely, which means It surrounds it on every side (medially, laterally, and anteriorly, but not posteriorly) • Under the tunica vaginalis lies the thick fibrous capsule (tunica albuginea) • The tunica vasculosa is the inner vascular layer of the tunica albuginea • In the posterior margin of the testis ,the tunica albuginea forms the mediastinum of the testis ,Connective tissue septae originate from the mediastinum, it passes into the interior of the testis, and it divides the parenchyma of the testis in about 370 conical lobules. • Each lobule contains sparse connective tissue with endocrine interstitial cells (or Leydig cells) secreting testosterone, convoluted seminiferous tubules (exocrine) in which sperm production occurs. Interstitial Tissue • The interstitial tissue of the testis between the seminiferous tubules consists of (Leydig cells) in sparse connective tissue and fibroblasts, lymphatics, and blood vessels including fenestrated capillaries • These cells produce the steroid hormone testosterone, which is responsible for stimulating and developing male genital organs and regulating the process of sperm production and the emergence of secondary sexual characteristics. Seminiferous Tubules • Germinal epithelium and lamina propria are the main components of the seminiferous tubule • Sperm are produced in the seminiferous tubules . • Each tubule is actually a loop linked by a very short, narrower segment, the straight tubule, to the rete testis . • Each seminiferous tubule is lined with a complex, specialized stratified epithelium called germinal or spermatogenic epithelium • The basement membrane of this epithelium is covered by fibrous connective tissue, with an innermost layer containing flattened, smooth muscle like myoid cells which allow weak contractions of the tubule • The germinal epithelium consists of two types of cells 1- Large nondividing Sertoli cells, which physically and metabolically support developing sperm cells • 2- Dividing cells of the spermatogenic cells namely (spermatogonia, primary and secondary spermatocytes, and spermatids ). They are located within the invaginations of Sertoli cells. Testis (cat). (1) Efferent ducts invested by connective tissue. (2) Tunica albuginea. (3) Rete testis. (4) Mediastinum testis. (5) Seminiferous tubules. H & E. ×20. Testis (bull). The seminiferous tubules are lined by a multilayered epithelium. (1) Spermatogonia. (2) Primary spermatocytes (3) Spermatids. (4) Developing spermatozoa. (5) Interstitial tissue separates the tubules. H & E. ×160. Spermatogenesis Stem cells called spermatogonia undergo mitosis and give rise to primary spermatocytes, which undergo a first meiotic division to form haploid secondary spermatocytes After a very short interval, secondary spermatocytes undergo the second meiotic division to produce small, round spermatids, which differentiate while still associated with Sertoli cells. Spermiogenesis • Spermiogenesis, is the final phase of sperm production, is the temperature-sensitive process by which spermatids differentiate into spermatozoa • These involve flattening of the nucleus, formation of an acrosome that resembles a large lysosome, growth of a flagellum (tail), reorganization of the mitochondria in the midpiece region, and the loss of much of the cytoplasm Spermatogenesis of anamniotes • For anamniotes, spermatogenesis occurs in spermatocysts (cysts) which for most species develop within seminiferous lobules. • Cysts are produced when a Sertoli cell becomes associated with a primary spermatogonium. • Mitotic divisions of the primary spermatogonium produce a cohort of secondary spermatogonia that are enclosed by the Sertoli cell which forms the wall of the cyst. • With spermatogenic progression, spermatozoa is produced which are released, by rupture of the cyst, into the lumen of the seminiferous lobule. Photomicrograph of a semithin section of the testis of a frog. There are several cysts of developing sperm in this photograph. All of the ells in a cyst are at the same stage of development, 63 x. • Photomicrographs of sperm smears of various vertebrates showing the characteristic head shape associated with each species. • 1- Frog: crescent-like sperm head and a thin tail • 2-Turtle:narrow pointed head that is curved • 3-Chicken : the heads of chicken sperm are spindle-shaped and almost difficult to distinguish from the midpiece • 4- Rabbit :A clear head with a thin tail INTRATESTICULAR DUCTS • The intratesticular ducts are the straight tubules, the rete testis, and the efferent ductules • All of which carry spermatozoa and liquid from the seminiferous tubules to the duct of the epididymis • From the seminiferous tubules, sperm enter the short straight tubules that lead to channels of the rete testis in the mediastinum testis, then move via 15 or 20 efferent ductules • Rete testis has a simple squamous E • The efferent ductules are lined pseudostratified epithelium, and some of the cells are ciliated • A thin layer of circularly oriented smooth muscle cells in the walls of efferent ductules aids sperm movement into the duct of the epididymis EXCRETORY GENITAL DUCTS • EXCRETORY GENITAL DUCTS are (Epididymis, The ductus (or vas) deferens and The urethra) • They transport sperm from the Testis to the penis during ejaculation • 1-Epididymis : Its long, coiled duct, surrounded by connective tissue • it includes a head region where the efferent ductules enter, a body, and a tail opening into the ductus deferens. • The epididymal duct is lined with pseudostratified columnar epithelium with characteristic long stereocilia. • The duct epithelium is surrounded by a few layers of smooth muscle cells, arranged as inner and outer longitudinal layers as well as a circular in the tail of the epididymis • In it ,sperm undergo maturation and short-term storage • Peristaltic contractions move the sperm along the duct. • 2- ductus (or vas) deferens • From the epididymis the ductus (or vas) deferens, a long straight tube with slightly folded mucosa that epithelial lining is pseudostratified with stereocilia and a relatively small lumen • The very thick muscularis consists of longitudinal inner and outer layers and a middle circular layer. • The muscles produce strong peristaltic contractions during ejaculation, which rapidly move sperm along this duct from the epididymis. • 3-Urethra • The male urethra can be divided into three sections: the prostatic urethra, the membranous urethra, the spongy urethra • The epithelial lining varies from urethelium proximal to the bladder, to stratified columnar along the greater part of the urethra, to stratified squamous at the tip of the penis • The urethra is the terminal part of the male duct system and has both a urinary and a reproductive function • The urethra carries urine from the bladder to outside of the body + expelling semen • Simple tubular mucosal glands open into the urethra. These are numerous in the horse, bull, boar and ram but sparse in the dog and cat ACCESSORY GLANDS ( just in mammals) • The accessory glands of the male reproductive tract produce secretions that are mixed with sperm during ejaculation to produce semen and that are essential for reproduction. • The accessory genital glands are the seminal vesicles (or glands), the prostate gland, and the bulbourethral glands. Seminal Vesicles • The two seminal vesicles consist of highly tortuous tubes, enclosed by a connective tissue capsule. • The mucosa of the tube displays a great number of thin, complex folds that fill much of the lumen • The folds are lined with simple columnar epithelial cells rich in secretory granules. • The lamina propria contains elastic fibers and is surrounded by smooth muscle with inner circular and outer longitudinal layers. • The seminal vesicles are exocrine glands in which production of their viscid, yellowish secretion (semen) • They are absent in carnivores and are true vesicles in the stallion . Prostate Gland • The prostate gland is a dense organ that surrounds the urethra below the bladder. • Prostate gland is a discrete compound tubular gland with a thick fibromuscular capsule extending into the gland • The epithelium is pseudostratified with tall columnar secretory cells • In the boar and ruminants, the prostate gland consists mostly of a disseminate portion in the form of a glandular layer in the submucosa of the proximal (prostatic) urethra. • It is well developed in the stallion and carnivore • It is absent in the ram and goat • In the dog the secretion is serous • in other animals it is seromucous Bulbourethral Glands • The paired round bulbourethral glands (or Cowper glands), are located in the urogenital diaphragm and empty into the proximal part of the penile urethra. • Bulbourethral glands are compound tubular and mucus secreting. • A Striated muscle capsule sends fine trabeculae into the gland • • • • • • • • PENIS The penis consists of three cylindrical masses of erectile tissue, plus the penile urethra, surrounded by skin Two of the erectile masses—the corpora cavernosa—are dorsal The corpora cavernosa are each surrounded by a dense fibroelastic layer, the tunica albuginea One of the erectile mass is corpus spongiosum surrounds the urethra Most of the penile urethra is lined with pseudostratified columnar epithelium. In end the corpus spongiosum expands, forming the glans. In the glans, it becomes stratified squamous epithelium Small mucus-secreting urethral glands are found along the length of the penile urethra. PENIS • In carnivores the terminal part of the corpus cavernosum ossifies to become the os penis (baculum) and contributes to the rigidity of the penis. • Small keratinized epidermal spines are present on the glans penis of the Cat • In most amniotes the intromittent organ contains erectile tissue that renders it turgid when gorged with blood • HEMIPENES of lizards and snakes are paired eversible sacs attached to the skin adjacent to the cloacal opening. • Crocodiles, turtles, and some birds have a single trough-like PHALLUS homologous with the penis of mammals. References • https://pubmed.ncbi.nlm.nih.gov/8 605396/ • https://www.jstage.jst.go.jp/article/ hsj2000/19/2/19_2_63/_pdf • https://pubmed.ncbi.nlm.nih.gov/2 048751/ • http://www.vivo.colostate.edu/hbo oks/pathphys/reprod/semeneval/m orph.html • Comparative Veterinary Histology, with Clinical Correlates PDF • Genital Systems PDF