Cell Structure and Function Two Basic Cell Types: Prokaryotic vs. Eukaryotic Cells Two Basic Types • Remember….cells are the basic unit of life for ALL living things. • There are two basic types of cells: 1. Prokaryotic cells – found in bacteria 2. Eukaryotic cells – found in protists, fungi, plants and animals As we discuss the differences between Eukaryotic and Prokaryotes fill in you concept map. Prokaryotes Eukaryotes Nut-Nucleus same No Nut-No Nucleus Characteristics Shared ONLY WRITE THE BOLDED WORDS IN VENN DIAGRAM • same basic functions • Plasma membrane to control what enters and leaves the cell • “Filled” with cytoplasm • Contain ribosomes to make protein • Contain DNA What Makes Eukaryotic Cells Different? ONLY WRITE THE BOLDED WORDS IN VENN DIAGRAM • Much larger • Much more complex • Contain a nucleus to house the genetic material (DNA) • Linear DNA packaged into chromatin found inside the nucleus • Contains organelles-specialized structures in the cytoplasm • Not all have a cell wall What Makes Prokaryotic Cells Different? ONLY WRITE THE BOLDED WORDS IN VENN DIAGRAM • • • • • • Much smaller Less complex No nucleus Circular DNA that is found in the cytoplasm No organelles found in the cytoplasm Surrounded by a cell wall Eukaryotic Cells What does size have to do with it? • Prokaryotic cells are much smaller than eukaryotic cells. Why? – Smaller surface area to volume allows nutrients to easily and quickly reach inner parts of the cell. – Eukaryotic cells are larger and can not pass nutrients as quickly. They require specialized organelles to: • carry out metabolism • provides energy • transport chemicals throughout the cell Why do we call them cells? • In 1665, Robert Hooke was the first to view cells from cork (dead plant material). • He called them “cells” because they looked like tiny rooms. Cell Theory 1. All living things are made of CELLS 2. CELLS are the basic units of structure and function in living things 3. New CELLS are produced from existing CELLS About Cell Membranes 1.All cells have a cell membrane 2.Functions: a.Controls what enters and exits the cell to maintain an internal balance called homeostasis b.Provides protection and support for the cell TEM picture of a real cell membrane. 3.Structure of cell membrane Lipid Bilayer -2 layers of phospholipids a.Phosphate head is polar (water loving) b.Fatty acid tails non-polar (water fearing) c.Proteins embedded in membrane Phospholipid Lipid Bilayer • 4. Cell membranes have pores (holes) in it a.Selectively permeable: Allows some molecules in and keeps other molecules out b.The structure helps it be selective! Pores Polar heads Fluid Mosaic love water Model of the & dissolve. cell membrane Non-polar tails hide from water. Carbohydrate cell markers Proteins Membrane movement animation Label the cell membrane in your notes Outside of cell Proteins Lipid Bilayer Transport Protein Animations of membrane Go to structure Section: Carbohydrate chains Phospholipids Inside of cell (cytoplasm) •Animations of Active Transport & Passive Transport Types of Cellular Transport • Passive Transport cell doesn’t use energy 1. Diffusion 2. Facilitated Diffusion 3. Osmosis • Weeee!! ! high low Active Transport cell does use energy 1. Protein Pumps 2. Endocytosis 3. Exocytosis This is gonna be hard work!! high low Passive Transport • • • cell uses no energy molecules move randomly Molecules spread out from an area of high concentration to an area of low concentration. • (HighLow) • Three types: 3 Types of Passive Transport 1. Diffusion 2. Facilitative Diffusion – diffusion with the help of transport proteins 3. Osmosis – diffusion of water Passive Transport: 1. Diffusion Simple Diffusion Animation 1. Diffusion: random movement of particles from an area of high concentration to an area of low concentration. (High to Low) • Diffusion continues until all molecules are evenly spaced (equilibrium is reached)-Note: molecules will still move around but stay spread out. http://bio.winona.edu/berg/Free.htm Passive Transport: 2. Facilitated Diffusion A 2. Facilitated diffusion: diffusion of specific particles through transport proteins found in the membrane a. Transport Proteins are specific – they “select” only Facilitated certain molecules to cross diffusion the membrane (Channel Protein) b.Transports larger or charged molecules Carrier Protein B Diffusion (Lipid Bilayer) Passive Transport: 3. Osmosis Osmosis animation • 3.Osmosis: diffusion of water through a selectively permeable membrane • Water moves from high to low concentrations •Water moves freely through pores. •Solute (green) to large to move across. • Hypotonic Solution Osmosis Animations for isotonic, hypertonic, and hypotonic solutions Hypotonic: The solution has a lower concentration of solutes and a higher concentration of water than inside the cell. (Low solute; High water) Result: Water moves from the solution to inside the cell): Cell Swells and bursts open (cytolysis)! • Hypertonic Solution Osmosis Animations for isotonic, hypertonic, and hypotonic solutions Hypertonic: The solution has a higher concentration of solutes and a lower concentration of water than inside the cell. (High solute; Low water) shrinks Result: Water moves from inside the cell into the solution: Cell shrinks (Plasmolysis)! • Isotonic Solution Osmosis Animations for isotonic, hypertonic, and hypotonic solutions Isotonic: The concentration of solutes in the solution is equal to the concentration of solutes inside the cell. Result: Water moves equally in both directions and the cell remains same size! (Dynamic Equilibrium) What type of solution are these cells in? A B C Hypertonic Isotonic Hypotonic Osmosis Video • Osmosis Rap Osmosis worksheet Active Transport •cell uses energy •actively moves molecules to where they are needed •Movement from an area of low concentration to an area of high concentration •(Low High) •Three Types: 3 Types of Active Tranport • 1. Protein Pumps • 2. Endocytosis: • 3. Exocytosis Types of Active Transport 1. Protein Pumps transport proteins that require energy to do work •Example: Sodium / Potassium Pumps are important in nerve responses. Sodium Potassium Pumps (Active Transport using proteins) Protein changes shape to move molecules: this requires energy! Types of Active Transport • 2. Endocytosis: taking bulky material into a cell • Uses energy • Cell membrane in-folds around food particle • “cell eating” • forms food vacuole & digests food • This is how white blood cells eat bacteria! Types of Active Transport 3. Exocytosis: Forces material out of cell in bulk • membrane surrounding the material fuses with cell membrane • Cell changes shape – requires energy • EX: Hormones or wastes released from cell Endocytosis & Exocytosis animations Exocytosis and Endocytosis Exocytosis – process of a cell releasing materials (exiting) Endocytosis – process of cell taking materials in (entering) Exocytosis How Organisms Deal with Osmotic Pressure • Paramecium (protist) removing excess water video •Bacteria and plants have cell walls that prevent them from over-expanding. In plants the pressure exerted on the cell wall is called tugor pressure. •A protist like paramecium has contractile vacuoles that collect water flowing in and pump it out to prevent them from over-expanding. •Salt water fish pump salt out of their specialized gills so they do not dehydrate. •Animal cells are bathed in blood. Kidneys keep the blood isotonic by remove excess salt and water. Cellular Transport Review Diagrams Active Transport Examples Diffusion/ Facilitated Diffusion Osmosis Cell Transport Concept Map Name Date Cell transport involves the exchange of molecules through the Cell membrane Against the concentration gradient (low to high) Active transport requires ATP: Energy Down the concentration gradient (high to low) Passive transport large molecules transported by small molecules move through Proteins moved by Endocytosis in Diffusion Exocytosis out Small molecules move through of water Larger molecules move through Osmosis examples Example: Glucose Amino acids Ions Word Bank: Active Transport Passive Transport Diffusion Food Bacteria Facilitated Diffusion ATP Energy Glucose/Amino Acids/Ions Wastes/Secretions Example: Wastes Secretions Cell membrane Proteins also called Cell Membrane (2X) Osmosis Food/Bacteria Proteins (2X) Endocytosis Exocytosis Facilitated Diffusion