QUATERNARY AMMONIUM SILANE FOR ANTI-MICROBIAL EFFICACY
 Odorless
 Eco-responsibility
 Surface compatibility
 Broad spectrum - shields the surface against bacteria, viruses, molds,
fungi, yeast and protozoa
 Stable on storage
 Non- flammable
 Retentive for 90 days (under simulated conditions)
 Non-toxic and safe
 Cost-Effectiveness
Antimicrobial, Disinfectant, Biocide, Sterilant, Biostatic and Odor Eliminator
Is based on advanced silane chemistry : the organo silane technology is
proven to be very effective
It is a is the next generation retentive “ anti-microbial ” technology that
works on all surfaces with the added benefit of applying through water
solution.
Traditional antimicrobials provide short-term kill of microorganisms.
Unlike disinfectants, sanitizers and bleaches, the water based
Saniguard-7500 forms a molecular bond to surface. The molecule
disrupts the cell membranes of harmful fungi and bacteria, inhibiting,
reducing and providing long-term, residual kill.
It can produce a durable, non-migrating antimicrobial finish to a wide
range of textiles. SANIGUARD-7500 can form an invisible and durable
micro-biostatic coating on a broad range of hard surfaces.
The physical, rather than chemical, mode of action of SANIGUARD-7500
does not affect normal skin flora, does not lose strength with use and
does not promote adaptative organisms (super bugs)
Sword That Punctures
The Cell Membrane Of All Microbes
SANIGUARD-7500 : Mode of action
Part I : The first part of the molecule is the silane base. This silane base is the antimicrobial
anchor. The antimicrobial is anchored by covalent bonds which are formed gradually through
hydrolysis reactions that bond the antimicrobial permanently to almost any surfaces and allow
for crosslinking and polymerisation with other molecules.
Part II : The second part of the molecule is the centrally located positively charged nitrogen. It
plays an important role in the active nature of the antimicrobial. Some membranes of microbes
are negatively charged; when in close proximity, the microbes are drawn into the active surface
of the antimicrobial and
Part III : The third segment is the edge that leads to blow in the offending microbes. This long
molecular chain acts like a sword that punctures the cell membranes of all microbes coming in
contact with it.
SANIGUARD-7500 : Mode of action
When a microorganism comes in contact with the treated surface, the
C-18 molecular sword punctures the cell membrane and the electrical
charge shocks the cell. As the microbe is further drawn into the
antimicrobial, it is electrocuted
Since nothing is transferred to the now dead cell, the antimicrobial
doesn’t lose strength and the sword is ready for the next cell to contact it.
The active layer remains intact, efficacious and ready for the next cell
that approaches.
Summary
When the antimicrobial is bonded to the surface of an object, it is ready for defense.
As the microbes come into contact with the “antimicrobial” surface, they are first punctured
by the long molecular chain.
The “positive-negative” attraction in between the positively charged nitrogen and the
negative cell membrane draw the microorganism even further down on the sword.
This modified cell can end the life of a microbe but the end point is the contact between the
negative and the positive charges : the cell membrane is electrocuted and blown apart
delivering inescapable kill
In addition, since this process transfers nothing from the antimicrobial to the cell, the
antimicrobial stands ready for the next “wave” and can continue to work at full strength and
definitely.
Applied in a single stage of the wet finish process, the
attachment of SANIGUARD-7500 to surfaces involves two
means.
First and the most important is a very rapid process, which coats
the substrate , with the cationic species (physics sorption) one
molecule deep. This is an ion exchange process by which the
cation of the silane quaternary ammonium compound replaces
protons from water or chemicals on the surface.
The second mechanism is unique to materials such as silane
quaternary ammonium compounds. In this case, the silanol
allows for covalent bonding to receptive surfaces to occur
(chemisorptions). This bonding is then made even more durable
by the silanol functionality, which enables them to
homopolymerize. After they have coated the surface in this
manner, they become virtually irremovable, even on surfaces
with which they cannot react covalently.
1. Molecule used in SANIGUARD-7500
2. Surface view
3. Treated surface
We have 2 reactions:
1. The product forms a molecular BOND to any fabric
2. Product forms a film : crosslinking of the product
Saniguard 7500 : Solution Preparation : For exhaust & spray
Add one to three part of Saniguard 7500 to 97-99 parts of water
and stir the solution for 5 min before using.
Use the solution after preparation within 7 days
Do not use as hand sanitizer or spray on humans, animals and
avoid inhalation
High Touch Surfaces
Coatings & Surface Treatments
Textiles
Construction Material
Classification
Textiles
Household goods
Construction material
Footwear
Application
Socks, underwear, dress-shirt, working uniform, formal dress Bedding &
Night clothes (filling of a quilt, quilt cover, sheet, pajamas, blanket) Gloves,
hat, handkerchief, apparels, mask etc
Kitchen goods (towel, dish cloth, chopping board, water purifier, sponge),
Polyurethane foam for household, industrial and institutional sponges and
mops.
Bath room & toilet goods (bathtub, washbowl, bath mat, toothbrush, toilet
fixtures, toilet seat cover, tiles, granite, paints, wood , sunmica, glass, plastic
etc)
Paints, granite, wood, metal, plastic, glass, marble, ceramic, rubber, resins,
cement surfaces etc, Roofing materials – defined as shingles, roofing
granules, wood shake, felt stone & synthetic overcoats.Sand bags, tents,
tarpaulins, sails and ropes, jutes, Fiber glass duct board.
Shoes, Slippers, shoe insole
Interior goods
Home electric appliances
Carpet, curtain, upholstery.
Vacuum cleaner, air cleaner, washing machine, telephone, air conditioner,
headphones, floppy disk, key board, phones,switches , humidifier belts etc
Others
Stationary (Ball-point pen, file-cover, folder).
Cards (telephone card, cash card). Watch-strap, belt etc cell phones.
Computers, tabs, scrubs, masks, medical textiles, PPEs, medical equipment’s,
stethoscopes etc.
Disposable polyurethane foam cushions for lavidus air float systems.
Polyurethane foam for household, industrial and institutional sponges and
mops.
Polyurethane foam for packaging and cushioning in non-food contact
applications Polyurethane foam used as a growth medium for non-food crops
and plants
Hard and finished, both porous and non-porous surfaces, all medical equipment,
medical instruments and devices, medical furniture, glass, rubber, stainless steel,
concrete, plastic, drywall, marble, ceramic, supplies, medical textiles garment and
much more!.
All Medical Equipment
Saniguard 7500 is a nano-biostatic surface coating which protects against harmful
bacteria and viruses. Durable, invisible, odourless, and will not affect the performance of
the equipment.
Office and Work Spaces:
Furniture, High touch surfaces, HVAC, Ventilation, Heating and Air Quality,
Washrooms, Showers, Changing Areas, Break rooms, Lobbies and Common Meeting
Areas.
In Healthcare:
Hospitals, Operating Theater, Medical Instruments, Patient Rooms, Medical Furniture, Hospital
Common Area, Washrooms, Showers, Door, Windows, Floor, Walls, Clinics, Chiropractor Offices,
Physical Therapy Facilities, Massage Centers, Dental Offices and Extended Care Facilities, Ambulance
and Patient Transportation, Medical Equipment and Devices, Medical Clothing, Scrubs, Lab Coat,
Towels and Washcloths, Bottom Sheets, Sleep Knit Envelope Sheets, UltraKnit, Envelope Sheets,
Privacy Curtains, Shower Curtains, Stretcher Sheets, Bariatric Sheets, Crib & Bassinette Sheets,
Birthing Bed Sheet, Ultra Knit and Sleep Knit Top sheets, Ultra Knit and Sleep Knit Pillowcases,
Woven Flat Sheets & Pillowcases, Peri Cloths, Aprons, Impermeable Aprons, Discloths and Tea
Towels, Tablecloths, Napkins and Placemats, Color Grid, Knit Contour, Thermal Spread Blanket,
Spread, Thermal Blanket, Spread, Thermal Blanket, Ether Blanket, Patient and IV Gowns, Patient
Gowns, Precaution Gowns, Aurorra Clothing Protectors, Perma Print Clothing Protectors, Terry &
Twill Clothing Protectors, Synthetic Bedpads, Aurorra Bedpads, Chamonix Bedpads, Meridian
Bedpads, Sonoma Bedpads, Cotton Bedpads, Chairpads, Laundry Bags, Vinyl Laundry Bags, Mesh
Laundry Bags, Zippered, Mesh Laundry Bag, Ward Carts, PVC Mesh Bags and Cart Covers, Soiled
Linen Laundry Carts and High touch surfaces and more.
Customized Solutions:
Schools, University, College, Libraries, Spas and Aesthetic studios, Community
centers, Vet Clinics, Malls and Shopping Centers, Entertainment, Buses, Trians, and
other forms mass transportation, Government Facilities, Public Transportation,
Hotels, Restaurant, Airports and Airplanes.
Sports and Recreation:
Gyms and Fitness Facilities, Equipment, Washrooms, Showers, Locker Rooms and
Changing Areas, Steam Rooms, Hot Tubs, Swimming Pool, and Other Aquatic
Areas, Artificial Grass and Turf, Yoga studios, High Touch Surfaces and much much
more!
Implementation
Saniguard 7500 can be seamlessly integrated into the Hospital acquired infections (HAI), Work
acquired infections (WAI) and cross-contamination reduction protocol in the following ways:
•
Disinfection using non-mutagenic and water-based chemicals with shortest kill times.
•
Surface protection following disinfecting regimen by spraying or wiping down the surface with
water- based Saniguard 7500 solution. This includes un-obvious hotspots such as elevator
buttons, handrails, countertops, office equipment, medical test equipment, cell phones,
computers Tabs, etc.
•
Spraying all surfaces such as walls and ceilings to allow Saniguard 7500 treatment to scrub the
air by applying in Air-handling units (AHU), Air-vent filters, HVAC systems etc.
•
Specifying material treatment of textiles in hospitals including scrubs, bed-linens, curtains,
towels, mattress covers, gowns, Masks, etc.
Implementation Contd
•
Replenishing treatments on a regular schedule.
•
Specifying Saniguard 7500 treatment, where possible, at the OEM level in key contamination
vectors such as stethoscopes, medical device housings, textile furnishing, glass windows,
distancing barrier sheets, Face shields, cough-guards, nurse-stations, reception areas etc.
•
Applying Saniguard 7500 dilutions (aqueous or in alcohol) on all Rigid-Flexible, PorousNonporous surfaces i.e plastics, wood, metal, rubber, paper, ceramics, stone ,porcelain, glass,
leather, textile, garments, etc.
•
Replenishing treatments on a regular schedule.
•
Specifying Saniguard 7500 treatment, where possible, at the OEM level in key contamination
vectors such as stethoscopes, medical device housings, textile furnishing, glass windows,
distancing barrier sheets, Face shields, cough-guards, nurse-stations, reception areas etc.
•
Applying Saniguard 7500 dilutions (aqueous or in alcohol) on all Rigid-Flexible, PorousNonporous surfaces i.e plastics, wood, metal, rubber, paper, ceramics, stone ,porcelain, glass,
leather, textile, garments, etc.
Can be applied by:
Padding , exhaust , Soaking, Spraying , Fogging, Dipping, Brushing or wiping
SURFACE APPLICATION METHOD:
Clean the solid surfaces with mild detergent and water before spraying Saniguard 7500 solution.
Spray the solution on surfaces using a high-pressure mist sprayer/fogger only with dilution to
ensure the deposition of finer atomized droplets and better surface coverage. Before mist spray
check the effect and re-wetting on a small trial area before proceeding. If you experience a sticky
surface then increase the dilution and after misting hand mopping should be done.
For very smooth granite, marble, Kota stone, SS surfaces, and smoothly coated walls, application
with hand mopping is recommended.
High-pressure mist sprayer/fogger/hand mopping is recommended for exterior application & For
interior application fogger & hand mopping is recommended.
SURFACE APPLICATION METHOD Contd:
Allow the treated surface to dry at room temperature overnight . The treated surface now has a
retentive disinfectant layer.
Note Saniguard 7500 active has reactive Silane quat and forms a bonded protective structure on the
surface to be coated. Hence always do a trial with the suggested applicator and method before
usage.
Use facemask, goggles and gloves before applying the product
TEXTILE APPLICATION METHOD:
All clothing and other textile products should be treated by padding or exhaust (soaking ) them for
at least 15 minutes in Saniguard 7500 solution, followed by wringing or squeezing the textile
material and drying at room temperature. Use Saniguard 7500 solution post detergent wash.
Air control : Filter & Air Ducts (metallic materials)
Concrete industry
Paints
AIR CONTROL : Filter & AIR DUCTS (metallic materials)
SANIGUARD-7500 on FILTER
Provides an invisible durable microbiostatic coating on most substrates from a water-based
system.





Inhibits the growth of odor causing bacteria.
Prevents the growth of mold/mildew and algae.
Is compatible with many processes and conditions, including pH from 3 to 10.
Attacks malodors at their source to prevent decay.
Can be incorporated into the manufacture of a wide variety of materials, providing extended
life and usefulness to many products.
Uses
Air purification systems.
Automobiles.
Re-circulating air handling systems.
Vacuum cleaner filters.
Aquariums.
Application level : Generally applied by spray : a 1-3% solution
Has 6 months protection against mold, mildew, fungus and algae
Is superior protection from moisture penetration and the effect of weathering
Can be cleaned up easily , and is non-toxic to humans, animals and vegetation
Resists staining and water penetration and even resists spider webbing
Dries to the touch in 1 to 2 hours, may be recoated in 3 to 4 hours depending on condition
Application level
Spray over air ducts(metalic materials)
Mix thoroughly before use, easily applied with a quality brush, roller, airless spray and low
pressure hand pump sprayer
Always apply material to test panel prior to application
Dry to the touch in 1 ¡V 2 hours, may be recoated in 3 ¡V 4 hours depending on condition
Dilution Ratio 1 : 5 pure water. 200 litres = 1,000 litres of ready to use(RTU) products
CONCRETE INDUSTRY
SANIGUARD-7500 in Concrete Industry
Prevents deterioration due to fungi (mold and mildew), bacteria and algae.
Prevents stains caused by fungi (mold and mildew), bacteria and algae.
Prevents odors caused by fungi (mold and mildew), bacteria and algae.
Prevents deposition of dust on the treated surfaces.
Is easy to apply and more effective than traditional antimicrobial products.
Application level
30 ml to 90 ml / litre of water. This amount shall be included in total water of
the concrete mix design (for wet mix concrete).
Paints Industry
Saniguard 7500 can be used in wood , decks painting, latex indoor and outdoor
paints and stains; woodstains, architectural paints; lacquer and maintenance
coatings; films; laminates and finishes including alkyd, urethane, enamel, epoxy,
siloxaline, amino resins, textile coatings, extrusion coatings, architectural
coatings and overlays, anti-corrosion coatings fire-resistant coatings, aliphatic
coatings, vinylester and polyester coatings, gel coatings, aminoresins, resins
used as additive mixes for cement, epoxy laminating resins.
By incorporating SANIGUARD-7500 in the Wood for wood furniture will
inhibit deterioration and damage cause by these microorganisms for an
extended period of time.
1. Analysis of application of saniguard-7500
Correct Application of SANIGUARD-7500 can be checked by two means.
1. Bromophenol. Blue indicator-indicates the presence of SANIGUARD7500 by a blue cast. If no SANIGUARD-7500 is present, no color will
form.
2. Phydron quaternary Test Strips QT-10 theses strips indicate the presence
of a quaternary compound. These can be used to determine the
effectiveness of the bath treatment. Use them as you would pH paper and
read the amount of quaternary compound, if any that has been left in the
bath.
2. To prevent yellowing of the fabrics where saniguard-7500 is used
To prevent the Discoloration or yellowing of fabrics it is necessary to control
the pH of the bath. Alkalinity may prohibit SANIGUARD-7500 from
attaching to fabric. PH of fabric should be 4.5- 5.5 to aid exhaustion.
Different Acids solution can be used for this process. The most common
used are:
• Acetic Acid
• Citric Acid
How to determine if SANIGUARD-7500 is present ?
Bromophenol Blue (BPB) stain test
Soak the treated substrate in BPB for 5 mins (0.04%
solution in Water/ Methanol 80/20 )
Rinse the substrate with copious amount of water
Compare the remaining blue stain on the substrate to the
untreated control and color standard (to be done)
OVERTREATED
SLIGHTLY OVERTREATED
GOOD TREATMENT
SLIGHTLY UNDERTREATED
UNDERTREATED
Safety
Non-toxic and safe
Effectiveness
Broad-spectrum efficacy against bacteria, viruses, molds, fungi, yeast and protozoa
Cost-Effectiveness
It is competitive in cost and a significant improvement over current offerings in effective
cost when potential for recontamination or cross-contamination are factored.
Currently, non-leaching anti-microbials represent the best available solution. Within these,
certain silver and copper-based chemicals have shown limited anti-viral activity,
discolouration and have been expensive. Nano- silver has not been evaluated for long-term
health and environmental issues.
Si-Quats have proven efficacy, non-mutagenicity and durability. Earlier versions of these
were limited in their applicability and safety because of solvent content. This restricted use
in mainstream cleaning and protecting regimens. Over the years, innovations in process
chemistry have allowed Saniguard 7500 to be diluted into stable water-based formulations.
This has allowed all of the efficacy, non-mutagenicity and durability benefits of the SiQuat chemistry with safety and eco-responsibility.
This allows Saniguard 7500 be easily applied as fabric treatments, protecting solutions and
surface treatments for contaminations discussed earlier such as cell phones. Computers,
tabs, scrubs, masks, medical textiles, PPEs, medical equipment’s, stethoscopes etc.
Durability:
Durable and continue to act effectively for several days to weeks to
months to life of the substrate (depends on application methods) – it
protects contamination vectors between cleaning regimens and
is durable enough to resist removal by these regimens be they
disinfection, sanitization or laundering
Non-mutagenicity
Does not create super-bugs by using a physical killing mechanism
and thus removing the poisoning cycle that leads to resistance in
microorganisms
Eco-responsibility
It is water-dilutable and has no volatile organic emissions (VOCs)
and have no adverse environmental impact during its entire life
cycle.
The following table lists the pathogens that are killed or inactivated following application
of Saniguard 7500 to various substrates and materials.
PATHOGENS INACTIVATED BY SI-QUAT APPLICATION
Gram Positive Bacteria
Bacillus sp. (vegetative cell)
Corynebacteriumdiptheriae
Micrococcus lutea
Micrococcus sp.
Mycobacterium tuberculosis
Mycobacterium smegmatis
Propionibacterium acnes
Staphylococcus aureus *
Staphylococcus epidermidis
Streptococcus faecalis
Streptococcus mutans
Streptococcus pneumonia
Streptococcus pyrogenes
Gram Negative Bacteria
Acinetobacter calcoaceticus
Aeromonas hydrophilia
Citrobacter deversus
Citrobacter freundi
Enterobacter aerogenes
Enterobacter aglomerans
Enterobacter cloacae
Enterococcus
Escherichia coli
Klebsiella oxytoca
Klebsiella pneumoniae
Klebsiella terriena
Legionella pneumophila
Morganella morganii
Proteus mirabilisl
Proteus vulgaris
Pseudomonas aeruginosa
Pseudomonas fluorscens
Salmonella cholera suis
Salmonella typhi
Salmonella typhimurium
Serratia liquifaciens
Serratia marcescens
Xanthomonas campestris
Viruses
Adenovirus Type II & IV
Bovine Adenovirus Type I & IV
Feline pneumonitis
Herpes Simplex Type I
Herpes Simplex Type II
HIV-1 (AIDS)
Influenza A2 (Aichi)
Influenza A2 (Asian) Influenza B
Mumps
Parinfluenza (Sendai)
Rous Sarcoma
Reovirus Type I
Simian Virus 40
Vaccinia
MS2 PRD1
Viruses
Fungi , Algae , Yeast
The following table lists the pathogens that are killed or inactivated following
application of Saniguard 7500 to various substrates and materials.
Fungi, Algae, Mould, Yeast, Spores
Alterania alternate
Aphanizomenon sp.
Aspergillus flavus
Aspergillus niger
Aspergillus sydowi Aspergillus terreus
Aspergillus versicolor
Aspergillus verrucaria
Aureobasidium pullans
Candida albicans
Candida pseudotropocalis
Chaetomium globsum
Cladosporium cladosporioides
Chlorella vulgaris
Dreschslera australiensis
Epidermophyton sp.
Gliomastix cerealis
Gloeophyllum trabeum
Microsporum sp.
Microsporu maudouinii
Monilia grisea Oscillatoria
Penicillium chrysogenum
Penicillium commune
Penicillium funiculosum 14
Penicillium pinophilium
Penicillium variable Phomafimeti
Pithomyces chartarum
Poria placenta
Scenedesmus
Saccharomyces cerevisiae
Scolecobasidium humicola
Selenastrum sp.
Trichoderma viride
Trichophyton interdigitale
Trichophyton maidson
Trichophyton mentogrophytes
Trichophyton sp.
Protozoa Parasites
Cryptosporidium parvum (oocysts)
Antimicrobial Activity Evaluation Methods
1. MIC
2. AATCC Test Method 30
3. AATCC Test Method 90 (Halo Test)
4. AATCC 100 / ISO 20743 / ISO 20645
5. Modified AATCC 100 with MS2 Bacteriophage (Antiviral for textiles)
6. JIS Z 2801 / ISO 22196
7. JIS L 1902
8. ISO 18184(Antiviral for textiles)
Features of Various Antimicrobial Agents for Textile
Classification
Treatment
Method
Durabilit
y
Safety (LD50)
Leach/No
n-leach
Saniguard 7500
Dilute with water
Excellent
12,270mg/kg
Non-leach
Quaternary
Ammonium
Compounds
Mix with reactive
resin for fiber
Fair
300-400 mg/kg
Leach
Biguanide
Mix with reactive
resin for fiber
Chitin, Chitosan
Mix with reactive
resin for fiber
Fair
>5,000mg/kg
Leach
Inorganic Silver
Mix with raw
materials of
synthetic fiber
Excellent
>2,000mg/kg
Slowrelease
Natural extract
Microcapsule
Good
>2,000mg/kg
Slowrelease
Fiber
Inoculum
Fiber Saniguard-7500 treatment
Inocumum
Solved agent
Survived bacteria
Death bacteria
Death bacteria
(a) Water soluble type
(b)
Water non-soluble type [Saniguard-7500]
Untreated
Sample
LEACHABLE
Leaches out of substrate
to inhibit growth
DURABLE
Saniguard-7500
Durably bonded solid*
that inhibits growth by physical
contact
* The solid is a micro polymer
network
Sample of
Leaching
Antimicrobial
Bonded
Antimicrobial
Acute Oral Toxicity/LD50
One of the most typical toxicity evaluation tests in case of ingestion.
Test method/Using albino rats belong to mouse and feeding with test
samples. The number of fifty percent death was calculated from the
dead rat number statistically
Acute Oral Toxicity/ LD50
Saniguard 7500
Salt
Sorbic acid (food additive agent, disinfectant)
Caffeine (coffee, tea)
Sodium nitrife (a color former, disinfectant)
Ethylacohol (wine, whisky)
12.27
3.0
10.5
0.19
0.085
14
g/kg
g/kg
g/kg
g/kg
g/kg
g/kg
Acute Oral Toxicity
Albino Rats
Acute Dermal Toxicity
Albino Rat
Eye Irritation
Albino Rabbits
Primary Skin Irritation
Albino Rabbits
Four-Day static Fish Toxicity Studies
Rainbow Trout and Bluegills
Human Repeated Insult patch Test
Albino Rabbits
28-Day subacute Dermal Toxicity study
Acute vapor Inhalation Toxicity study
Albino Rats
Host mediated assay for detection of mutations induced
Three week vaginal irritation study
Beagle dogs
32-day human wear test for socks
Three month human wear test for athletic socks
Teratogenic study
Albino Rats
Mutagenicity evaluation
In vitro mammalian cell transformation assay in the absence of exogenous metabolic activation
Acute toxicity
Daphnia magna
Percutaneous absorption
Rabbits
Eight day dietary
Mallard duck
Acute oral toxicity
Mallard duck
Sock wear test/3 month human wear test
Test method:
Socks made of wool, cotton, nylon, acrylic were treated with
Saniguard-7500 and worn by 44 young men, American football
player, for 3-month continuously
Test result:
No observation of skin irritation or skin sensitization
Percutaneous absorption test
Test method:
Backs of rabbit wear clipped free, aqueous solution containing
14C-labeled Saniguard 7500 was applied. The Rabbits were kept
for ten days. 14C contents in urine and feces were analyzed
everyday. After ten- test-day, whole liver, kidneys, lung, spleen,
brain and gonads were removed and 14C content were analyzed.
Test result:
No observation of 14C in urine, feces and all organs. It proved that
Saniguard 7500 was not absorbed from the skin of rabbits.
1.Ceramic Tile
2.Granite
3.Sunmica
4.Wood
Name of Test : Test Method for Antimicrobial Activity of Hard Non-Porous Surfaces – JIS Z 2801:
2012
Test Organisms : Staphylococcus aureus ATCC 6538 & Escherichia coli ATCC 8739
Test Conditions :
Contact Time
Incubation Temp
Neutralizer Used
Media and Reagent
Incubation Period
:
:
:
:
:
24 Hours at 35°C +/- 1°C 2.
35°C +/- 1°C 3.
D/E Neutralizing Broth
TSA Agar
48 Hours
Note : A Value of 2.0 or above is considered “antimicrobial” by JIS
Where,
R=Value of antimicrobial activity
A = Average of the number of viable cells of bacteria immediately after inoculation on the untreated
test piece.
B = Average of the number of viable cells of bacteria on the untreated piece after 24 hrs.
C = Average of the number of viable cells of bacteria on the treated piece after 24 hrs
Remark : The sample shows antibacterial activity against both S. aureus & E. coil.
Results:
ACRYLIC, 2% Saniguard 7500
Sample
Identification
Parameter
Bacterial count after
0 hr (Cfu / Sample )
Treated
Un-treated
Treated
Un-treated
S. aureus
100
420
520
930
Treated
Un-treated
Treated
Un-treated
S. aureus
Treated
Un-treated
Treated
Un-treated
S. aureus
Treated
Un-treated
Treated
Un-treated
S. aureus
Treated
Un-treated
Treated
Un-treated
S. aureus
Treated
Un-treated
Treated
S. aureus
E coli
E coli
E coli
E coli
E coli
E coli
Log of 0
hr
Bacterial count after 24
hr (Cfu / Sample )
2
7600
2.623
820000
2.716
20000
2.968
3400000
WOOD , 2% Saniguard-7500
350
2.544
1
520
2.716
770
280
2.447
1
620
2.792
810
CERAMIC TILE , 2% Saniguard 7500
61000
5.785
111000
4800000
6.681
35000000
4000000
6.602
31000
77000000
7.886
5500000
GRANITE , 2% Saniguard 7500
140
2.146
1
2400
3.380
340
1200
3.079
1
3500
3.544
2000
PLASTIC SHEET, 2% Saniguard 7500
50
1.698
1
60
1.778
180000
80
1.903
35000
590
2.770
1050000
SUNMICA , 2% Saniguard 7500
1
0
1
130
2.113
110
680
2.832
1
Log of 24
hr
Anti –microbial(R)
R=[Log(B-C)]
3.880
5.913
4.301
6.531
2.02
0
2.886
0
2.908
2.8
5.045
7.544
4.491
6.740
2.49
0
2.531
0
3.301
2.5
0
5.255
4.544
6.021
5.3
0
2.041
0
2.04
2.23
2.9
2.24
3.3
1.5
2.78
 Keep surfaces hygienic for longer
without the hassle of sterilizing every
day!
OFFICE ADDRESS :
403, “Antariksh”, Makwana Road,
Marol Naka, Andheri (E),
Mumbai - 400 059
Tel.- 91-22-4221 6789 (30 Lines)
91-22-6692 4112
Fax -91-22-2836 6434
FACTORY ADDRESS :
D- 14 /10, TTC, MIDC Industrial Area,
Turbhe, P. O. K. U. Bazar,
Navi Mumbai - 400 705
Tel.-91- 22-4141 9899 (100 Lines)
Fax -91-22-4141 9888
Email ID: ketan@lnchem.in
nayana.lnchem@gmail.com
INTEGRATED MANAGEMENT SYSTEM
(IMS) CERTIFIED: ISO 9001 : 2015 ,
ISO 14001: 2015, ISO 18001 : 2007
GLOBAL REPORTING INITIATIVE GRI-G4
CERTIFIED CRISIL RATING : MSE 1*