CLINICAL SIGNIFICANCE Bone marrow examination help in detection of various diseases. 1.Aplastic anaemia 2.Megaloblastic 3.Sidroblastic anaemia 4.Acute type leukaemia 5.Chronic type of leukaemia PERFORMING BONE MARROW BIOPSY There are three methods of bone marrow biopsy. 1.Needle aspiration biopsy. 2.Microtrephin biopsy. 3.Surgical biopsy. NEEDLES:Various types of needles are used in BONE MARROW BIOPSY 1.Needle aspiration biopsy In this method salah or klema needles are used. 2.Microtrophine Biopsy In this method Jasshidi-swain or Islam trephine needles are used. 3.Surgical bone marrow biopsy This method is carried out under aseptic conditions in a operation theatre. SITE OF BONE MARROW BIOPSY 1.Sternum 2.iliac crest 3.Upper end of tibia in children below 2 year of age. Examination Immediately after aspiration of bone marrow, smears are prepared from the marrow material. Fix in methanol. Stain with any ROMANOWASKY stains. Differential cell count on bone marrow (Myelogram) is made.