C. elegans Writeup
Karmorreya Brown
TABLE OF
CONTENTS
EMPHASIS HEADING 1 .............. ОШИБКА! ЗАКЛАДКА НЕ
ОПРЕДЕЛЕНА.
Figure 1 .............................. Ошибка! Закладка не определена.
Table 1 ............................... Ошибка! Закладка не определена.
Figure 2 .............................. Ошибка! Закладка не определена.
Table 2 ............................... Ошибка! Закладка не определена.
Results ............................... Ошибка! Закладка не определена.
Gene
Figure 1 The three Possible C. elegan Dpy Strains and their gene, allele, mode of
inheritance and phenotypes
Allele
Mode of Inheritance
Additional Phenotypes
Dpy-3
e-27
X-Linked Recessive
Dpy-5
e-61
Autosomal Recessive (I)
Dpy-10
Cn-64
Autosomal Dominant (IT)
Alae morphology currant, emulate
morphology vacant, stunts variant
patterns of transgene expression
warrant
Antibody staining morphology
warrant body
Left handed roller
Table 1 Is showing a Cross Between 5 Males wildtypes and 1 Dpy Hermaphrodite. The
results show how many Wt Hermaphrodite and Dpy Hermaphrodite where present. In
addition to how many male Wt and Dpy males were present. In the end a total of four
plates were set up with one Dpy Hermaphrodite and five wildtype males.
Wt Hermaphrodite
Dpy Hermaphrodite
Wt Male
Dpy Male
7
30
11
14
12
7
5
7
7
6
7
13
6
10
5
22
25
18
4
37
10
26
3
20
16
20
6
10
10
15
5
16
5
16
3
4
3
6
2
7
Total 101
Total 141
Total 51
Total 150
Figure 2a Punnett Square of selfing in F1 X- Linked recessive
Xe27
O
Mutant100%
Figure 2a
Xe27
X+Xe27
Xe27
X+Xe27
Xe27O
Xe27O
WT-Recessive
Dominant
Mutant
Punnett Square of selfing in
Figure 2b Punnett Square of selfing in F1 X- Autosomal dominant which would result in
100% wildtype C. elegans
M
M
+
M
M+
+
M+
M+
+
100% Wildtype
Figure 2b Punnett Square of selfing in F1
XM
XM
X+Xm
X+Xm
X+
XmO
O
XmO
50% Wt Hermaphrodite
50% Dpy Males
Figure 2c Punnett Square of selfing in F1 which would be Autosomal recessive and result
in 50% being dpy and 50% being wildtype
XD
Xd
XDXD
XD Xd
XDY
2
XD Y
Xd Y
50% Affected 50%Unaffected
Table 2 Plate Results. After the cross between four plates containing 1 Dpy
Hermaphrodite and 5 wildtype males. The results show that there are more wildtype than
dpy in each of the plates which would mean that the dpy strain is possibly X- Linked
Recessive.
Table 2
Wild Type
Dpy
Plate 1
105
55
Plate 2
261
93
Plate 3
299
63
Plate 4
371
134
Total
1036
328
Results
The purpose of this experiment was to determine what the unknown strain of Dpy
given. There are three possible strains which included Dpy-3 which mode of inheritance
is X- linked Recessive, Dpy- 5 which mode of inheritance is Autosomal Recessive, and
the last strain is Dpy- 10 whose mode of inheritance is Autosomal Dominant. In (Figure
2a) we see that by doing the Punnett square the mode of inheritance is x linked dominant,
because the results showed that all off springs would be mutants. Further resulting in the
Hermaphrodite genotype being X+Xm and all the males being XmO. The next Punnett
square completed shows that the mode of inheritance is Autosomal Recessive which
would result in either all wildtypes, or 50% wildtype, or 50% mutant.
For (Table 1) we set up two discs. With each disc containing five wildtype males
and one Dpy hermaphrodite. The other disc containing two wildtype males and one Dpy
hermaphrodite. In each cross there seemed to be more wildtypes than Dpy which lead to
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our hypothesis being that the mode of inheritance for the given worms was autosomal
recessive which would make our worm type to be Dpy 5.
For Autosumal recessive to be possible the hermaphrodite had to be a carrier for
the Dpy allele. So when selfing the possible outcome for Dpy was 25 percent’ while
wildtype was 75 percent which would explain why there were fewer Dpy worms than
wildtype. In addition, our chi squared value was .467 and our p value was between
.50>X>.30 which supported our hypotheses that our worm type is autosomal recessive.
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