Molecules that Make Up Living Things Name ____________________ Organisms are made up of different molecules. The main molecules are water, carbohydrates, proteins, and fats (lipids). Each of these molecules is found in foods that we eat and each has an important job for the body. In this lab, you will learn how to conduct a chemical test to determine which of these compounds are found in different food samples. A. Monosaccharide- A type of carbohydrate 1. How many sugar units are monosaccharide composed of? ____ 2. What elements make up monosaccharides? ___________ 3. How does the body use monosaccharides? ___________ 4. Here is how you can test foods to see if they contain monosaccharides: a. Place a small sample (about 1 cm) of food (a light liquid is best) in a test tube. b. Put 10 drops of Benedict’s solution in the test tube. c. Place the test tube in a hot water bath for about 2 minutes. WEAR SAFETY GOGGLES WHILE HEATING TEST TUBES!!!!!!! d. A color change to orange, rust, or yellow indicates there are monosaccharides in the food sample. e. Let the test tubes cool and then put materials down the sink and clean the test tubes. 5. Choose 5 food samples to test for monosaccharides. Make a data table to record your results: 6. If you needed fast energy, which of the foods you tested would provide you with the monosaccharides required for this fast energy? _____________________________________ B. Disaccharides- Another type of carbohydrate. 1. How many sugar units are disaccharides composed of?_____ 2. What elements make up disaccharides? _________ 3. How does the body use disaccharides? ____________ 4. There is no chemical test for disaccharides. 5. Table sugar (white granulated sugar) is an example of a disaccharide. List some foods that contain a lot of disaccharides: _________________________________ C. Polysaccharides (starches)- ANOTHER TYPE OF CARBOHYDRATE!! 1. How many sugar units are polysaccharides composed of? ____ 2. What elements make up polysaccharides? ________ 3. How does the body use polysaccharides?_________________ 4. Here is how you can test foods to see if they contain polysaccharides (starches): a. Place a small sample of food in a container. b. Put a drop of iodine on the food sample. c. Wait 1-2 minutes. A color change to blue or black indicates there are polysaccharides in the food sample. 5. Choose 5 food samples to test for polysaccharides. Make a data table to record your results: 6. If you needed slow, steady energy, which of the foods you tested would provide you with the polysaccharides required for this slow, steady energy? ___________________________ D. Lipids (Fats) 1. What elements make up fats? __________________ 2. How does the body use fats? ___________________ ____________________________________________ 3. Here is how you can test foods to see if they contain fats: a. Place a small sample of food on paper towel and let sit for about 3 minutes. b. Remove the food and see if there is a translucent spot where the food was. If there is an “oil” spot the food sample contains fat. 4. Choose 5 food samples to test for lipids. Make a data table to record your results: 5. If you needed more insulation or padding, which of the foods you tested would provide you with the lipids required for this? ____________________________________________ E. Proteins 1. What are the building units that compose proteins?________ 2. What elements make up proteins?_________________ 3. How does the body use proteins?__________________ 4. Here is how you can test foods to see if they contain proteins: a. Put a small sample of food in the container. b. Place 10 drops of Buiret Reagent on the food and let sit for 1 minute. c. A color change from blue to purple (or lavender) indicates the food contains protein. 5. Choose 5 food samples to test for proteins. Make a data table to record your results: 6. If you needed materials for cell growth and cell repair, which of the foods you tested would provide you with the proteins required for this?_______________________________ __________________________________ F. Summarize things you learned from this lab?