1. Question 1 part Pili and inclusion of Volutin in bacteria.
1. Indicate in the figure pili _____, granules of Valyutin _____ Types of pili
___________________________________________________
2. The composition of pili ____________________________
3. Pili function __________________________________________
4. Volutin granules - chemical composition and functions
_______________________________________________________________________
_____________
5. Methods for staining Volutin inclusions (name the bacterium containing volutin
granules)
_______________________________________________________________________
_____________
2. Question 2 part Capsules and spores of bacteria.
1.
Indicate _____ capsule in the figure. The composition and properties of the capsule
_______________________________________________________________________
___,
_______________________________________________________________________
____
2. Function of the capsule
___________________________________________________________
3. Capsule staining methods (Examples of capsule-forming microorganisms)
_______________________________________________________________________
_____
4. Method of staining spores in bacteria
_______________________________________________________________________
_______________________________________________________________________
_____________________________
5. Examples of spore-forming bacteria
_________________________________________
3. Question 2 part Flagella of bacteria.
1. Indicate flagella in the figure ____ Flagella composition
_________________________________________________________________
2. Structure of flagella
_____________________________________________________________
3. Function of flagella
______________________________________________________________
4. Methods for the detection of flagella
___________________________________________________
5. An example of a monotrich _________________________________________
peritrich __________________________
4. Question 1 part You are shown the structure of the cell wall of the bacterial cell. You must
indicate - this is the cell wall of gram-positive or gram-negative bacteria?
_______________________________________________________________________
1. Give a detailed description of the presented cell wall - both in structure, in composition
and function..
_______________________________________________________________________
_______________________________________________________________________
_______________________________________________________________________
_______________________________________________________________________
_______________________________________________________________________
_______________________________________________________________________
_______________________________________________________________________
___
2. Cell wall function _______________________________________________________
_______________________________________________________________________
_______
3. The staining method used to differentiate the cell wall
_______________________________________________________________________
_______
4. What is protoplast
_______________________________________________________________________
_______________________________________________________________________
_______________________________
5. What is a spheroplast
_______________________________________________________________________
_______________________________________________________________________
______________
5. Question 1 part
1. Explain the nature of the growth of microorganisms. Indicate which in the figure.
2. facultative anaerobes because
_______________________________________________________________________
_______________________________________________________________________
______________
3. Obligatory anaerobes, because
_______________________________________________________________________
_______________________________________________________________________
______________
4. Microaerophiles, because
_______________________________________________________________________
_______________________________________________________________________
______________
5. Aero tolerant because
_______________________________________________________________________
_______________________________________________________________________
______________
6. Obligatory aerobes because
_______________________________________________________________________
_______________________________________________________________________
______________
1
2
3
4
5
6. Question 1 part Distribute the antibiotics described below according to their
mechanism of action :
Penicillin G, Amoxicillin, Flucloxacillin, Cefoxitin, Cefotaxime, Ceftriaxone, Imipenem,
Norfloxacin, Ciprofloxacin, Enoxacin, Ofloxacin, Gentamicin, Amikacin, Tetracycline,
Sulphonamides, Rifamycinum
1. Inhibits bacterial cell wall synthesis
_______________________________________________________________________
_______________________________________________________________________
____________________________
2. Inhibits bacterial protein synthesis
_______________________________________________________________________
_______________________________________________________________________
____________________________
3. Blocks bacterial cell metabolism by inhibiting enzymes
_______________________________________________________________________
_______________________________________________________________________
____________________________
4. Inhibits bacterial DNA replication and synthesis
_______________________________________________________________________
_______________________________________________________________________
____________________________
5. Inhibits the synthesis of bacterial mRNA
________________________________________________________
7. Question 1 part The infectious process is (give definition)______________________
_______________________________________________________________________
_______________________________________________________________________
1. How to determine the incubation period ___________________________________
____________________________________________________________________
____________________________________________________________________
2. Transmission ways for various infection __________________________________
____________________________________________________________________
____________________________________________________________________
3. Source of infection __________________________________________________
4. Define what an epidemic is______________________________________________
____________________________________________________________________
____________________________________________________________________
5. Infectious Disease Prevention ____________________________________________
____________________________________________________________________
____________________________________________________________________
8.
Question 1 part Features of morphology and cultural properties of bacteria.
1. Spherical bacteria ____________________________________________________
___________________________________________________________________
2. Rod-shaped bacteria ____________________________________________________
____________________________________________________________________
3. Inoculation method according to Drigalsky _________________________________
____________________________________________________________________
4. Gould’s inoculation method ______________________________________________
____________________________________________________________________
5. forms of colonies S – type_______________________________________________
____________________________________________________________________
9.
Question 1 part Methods for detecting the structural components of a bacterial cell..
1. Purpose Gram methods ________________________________________________
____________________________________________________________________
2. Purpose of Burri-Gins methods ___________________________________________
____________________________________________________________________
3. The purpose of the methods Romanovsky - Giemsa ___________________________
____________________________________________________________________
4. Purpose of the Ozheska method __________________________________________
____________________________________________________________________
5. The purpose of the Ziel - Nielsen method __________________________________
____________________________________________________________________
10. Question 1 part Sterilization, disinfection, aseptic and antiseptic. Antibiotics.
1. Definitions of sterilization ____________________________________________
2. Definitions of disinfection _____________________________________________
3. Definitions of aseptic ________________________________________________
4. Definitions of antiseptics _____________________________________________
5. What are the methods used to determine the sensitivity of microorganisms to
antibiotics
____________________________________________________________________
1. 2question (particular microbiology)
S aureus belongs to staphylococci
Morphology and Cultural
Virulence factors
tinctorial
properties 1.
properties
culture media
2. cultivation
conditions
t-? pH?
Principles and
features of the
laboratory
diagnostics
Diseases caused by
S aureus
2. 2question (particular microbiology)
Streptococcus pyogenes - streptococci
Morphology and Cultural
Virulence factors
tinctorial
properties 1.
properties
culture media
2. cultivation
conditions
t-? pH?
Principles and
features of the
laboratory
diagnostics
Diseases caused by
Streptococcus
pyogenes
3. 2question (particular microbiology)
Mycobactérium tuberculósis
Morphology and Cultural
Virulence factors
tinctorial
properties 1.
properties
culture media
2. cultivation
conditions
t-? pH?
Principles and
features of the
laboratory
diagnostics
Diseases caused
by
Mycobactérium
tuberculósis
Principles and
features of the
laboratory
diagnostics
Diseases caused
by
Brucella
melitensis
4. 2question (particular microbiology)
Brucella melitensis
Morphology and
tinctorial
properties
Cultural
properties 1.
culture media
2. cultivation
conditions
t-? pH?
Virulence factors
5. 2question (particular microbiology)
Corynebacterium diphtheriae
Morphology and Cultural
Virulence factors
tinctorial
properties 1.
properties
culture media
2. cultivation
conditions
t-? pH?
Principles and
features of the
laboratory
diagnostics
Diseases caused
by
Corynebacterium
diphtheriae
6. 2question (particular microbiology)
Salmonella enterica serotype typhi
Morphology and Cultural
Virulence factors
tinctorial
properties 1.
properties
culture media
2. cultivation
conditions
t-? pH?
Principles and
features of the
laboratory
diagnostics
Diseases caused
by
Salmonella
enterica
serotype typhi
7. 2question (particular microbiology)
Yersinia pestis
Morphology and Cultural
Virulence factors
tinctorial
properties 1.
properties
culture media
2. cultivation
conditions
t-? pH?
Principles and
features of the
laboratory
diagnostics
Diseases caused
by
Yersinia pestis
8. 2question (particular microbiology)
Clostridium tetani
Morphology and Cultural
Virulence factors
tinctorial
properties 1.
properties
culture media
2. cultivation
conditions
t-? pH?
Principles and
features of the
laboratory
diagnostics
Diseases caused
by
Clostridium
tetani
9. 2question (particular microbiology)
Treponema pallidum
Morphology and Cultural
Virulence factors
tinctorial
properties 1.
properties
culture media
2. cultivation
conditions
t-? pH?
Principles and
features of the
laboratory
diagnostics
Diseases caused
by
Treponema
pallidum
10. 2question (particular microbiology)
Flu Infectious Agent
Morphology and Cultural
Virulence factors
tinctorial
properties 1.
properties
culture media
2. cultivation
conditions
t-? pH?
Principles and
features of the
laboratory
diagnostics
Diseases caused
by
Flu Infectious
Agent
1. 3.question (situational task)
Distribute antibiotics according to their mechanism of action: Penicillin G, Amoxicillin,
Flucloxacillin, Cefoxitin, Cefotaxime, Ceftriaxone, Imipenem, Norfloxacin, Ciprofloxacin,
Ofloxacin, Gentamicin, Amikacin, Tetracycline, Sulphamamides, Sulphamamin
Inhibits the synthesis of the bacterial cell wall
_____________________________________________________________________________
_____________________
Inhibits bacterial protein synthesis
_____________________________________________________________________________
____________________________
It blocks the metabolism of bacterial cells by inhibiting enzymes
_____________________________________________________________________________
_____________________________
Inhibits bacterial DNA synthesis
_____________________________________________________________________________
______________________________________________________________________
Inhibits RNA synthesis_____________________________________________
_________________________________________________________________
2. 3.question (situational task)
Hepatitis D virus (HDV);
Delta infection is caused by a defective virus, which can propagate only in cells already infected
with some complete virus.
1. Indicate the virus that is involved in the replication of hepatitis D.
Explain why this virus cannot reproduce on its own
____________________________________________________________________________
2. Routes of transmission of HDV
_____________________________________________________________________________
3. Material for research
_____________________________________________________________________________
_____________________________________________________________________________
4. Methods of laboratory (microbiological) diagnosis of HDV
_____________________________________________________________________________
_____________________________________________________________________________
____________________________
5. Is HbS antigen a marker for HDV?
_____________________________________________________________________________
_____________________________________________________________________________
____________________________________________
3. 3.question (situational task)
Respiratory syncytial virus is the main cause of bronchiolitis and community-acquired
pneumonia in newborns.
1. Describe the virus genome _______________________________________________
2. Virus taxonomy ________________________________________________
3. Routes of transmission of the virus ________________________________________
4. Laboratory diagnostic methods __________________
5. Specific prevention _____________________________________________
4. 3.question (situational task)
A boy of 7 years old, fell ill acutely, with complaints of fever up to 38 C and headache.
The next day, a swelling appeared in the left parotid region, on the 4th day from the onset of
the disease, a swelling was noticed in the parotid region on the right.
The temperature and pain during chewing, dry mouth was maintained up to 3 days. A
preliminary diagnosis was made: Mumps.
1. Describe the genome of the virus ______________
2. Virus taxonomy ____________
3. Routes of transmission of the virus ________
4. Laboratory diagnostic methods _____________________________________
5. Specific prevention __________________________________________
5. 3.question (situational task)
For a patient with paroxysmal spasmodic cough to confirm the diagnosis of “Whooping cough”,
it is necessary to take material for the bacteriological examination by the method of “cough
plates”. Select a culture medium for inoculating material.
1. Describe the morphology of the bacterium _____________________
2. Features of the cultivation of bacteria ____________________________________
3. Routes of transmission ______________________
4. Laboratory diagnostic methods _________________________________
5. Specific prevention __________________________________________
6. 3.question (situational task)
A district pediatrician was called to a boy of the 9th grade. The boy was sicked the 3rd days
ago. During the examination, the doctor noted a high fever in the boy (38.20С), a dry, rough
cough, pain and lacrimation of the eyes, mucous membranes of the conjunctiva and
nasopharynx are hyperemic and irritated, nasal breathing is blocked. Filatov-Koplik spots are
on the mucosa of the buccal surface and upper palate. Preliminary diagnosis: "Measles,
catarrhal period of development"?
1. Describe the virus genome ______________________________________________
2. Virus taxonomy _________________________________________
3. Routes of transmission of the virus _______________________________________
4. Laboratory diagnostic methods ____________________________________
5. Specific prophylaxis _________________________________________
7. 3.question (situational task)
Several students have been admitted to the infectious diseases hospital. The students were
from the same class. At admission, all schoolchildren noted a condition as, severe meningeal
syndrome and a temperature of more than 40 ° C. Microscopy of the cerebrospinal fluid
revealed a large number of neutrophils containing bean-shaped diplococci.
1. Describe the morphology of the bacterium __________________________________
2. Features of the cultivation of bacteria ____________________________________
3. Routes of transmission ______________________________________________
4. Laboratory diagnostic methods ________________________________________
5. Specific prevention ___________________________________________
8. 3.question (situational task)
Several workers dined in the factory cafeteria. Pork cutlets seemed to them insufficiently cooked.
After 8-10 hours, they showed signs of acute gastroenteritis: nausea, vomiting, abdominal pain,
frequent loose stools and fever up to 38 ° C. Two workers in serious condition were hospitalized.
The doctor made a preliminary diagnosis: Salmonellesis.
1.
2.
3.
4.
5.
Опишитесь морфологии бактерии___________________
Особенности культивирование бактерии ____________________________________
Пути передачи __________________________________________________________
Методы лабораторной диагностики ______________________________
Специфическая профилактика _____________________________________
9. 3.question (situational task)
A 45-year-old patient was admitted to the infectious diseases hospital on the 5th day of
illness with complaints of decreased visual acuity, diplopia, and fog before the eyes.
Dry mouth, muscle weakness, and poor swallowing were observed.
On the eve of the disease was at a party where he ate canned home-made mushrooms. Based
on clinical symptoms, a diagnosis of Botulism was made.
1. Describe the morphology of the bacterium _______________________________________
2. Features of the cultivation of bacteria ____________________________________
3. Routes of transmission _____________________________________________________
4. Laboratory diagnostic methods _____________________________________
5. Specific prevention _________________________________________
10. 3.question (situational task)
In one of the villages, a boy from a large family, 6 years old, who fell ill 5 days ago, was brought
to the doctors. The disease was manifested by a sudden increase in temperature, severe
headaches. Repeated vomiting, pain in the arms and legs was observed. In the following days,
the condition of the child worsened. During the examination, the child observed meningeal
symptoms, muscle tone on the right leg was decreased, tendon reflexes were sharply weakened,
the foot dangled. Preliminary diagnosis: "Paralytic form of poliomyelitis"?
1. Describe the genome of the virus ______________
2. Taxonomy of the virus _________
3. Routes of transmission of the virus __________
4. Laboratory diagnostic methods ____________________
5. Specific prevention __________________________________________