Animal Cell and Plant Cell comparison lab : Do all drawings and any written work on your own paper. Do not write on this Lab sheet. Background info: READ in your group before you begin the lab activity. I. II. Cells: All living things are made of cells. Two types of eukaryotic cells ( cells with a nucleus) are Animal Cells and Plant Cells. Animal and Plant cells have many tiny organelles inside them. We can see some of these organelles with a compound light microscope. When viewing animal and plant cells, The main differences are: ANIMAL CELLS HAVE AN OUTER CELL MEMBRANE ONLY. PLANT CELLS HAVE A CELL WALL AS THE OUTER LAYER AND A CELL MEMBRANE AS THE INNER LAYER. PLANT CELLS ALSO HAVE CHLOROPLASTS WHICH CONTAIN THE GREEN PIGMENT CHLOROPHYLL. WHICH HELP THEM CONVERT SUNLIGHT INTO ENERGY DURING PHOTOSYNTHESIS BECAUSE PLANT CELLS MAKE THEIR OWN FOOD. ANIMAL CELLS DEPEND ON OTHER SOURCES FOR FOOD. ANIMAL CELLS DO NOT MAKE THEIR OWN FOOD. Using the microscope for this lab: after preparing a wet mount slide for each cell according to the procedure below, you will place the slide on the stage, turn on the light, and view each cell under low power. Focus with the course adjustment knob on low power only. Do not move to high power unless instructed to do so. I need to view your cell before you begin drawing and labeling organelles. Lab : Question: How is the animal cell different from the plant cell? Hypothesis: ( Answer the question above based on what you think before the lab.) Use the I think… because…. Way to answer . Procedure: Wet mount animal cell: One group member should gently roll a tooth pick on the inside of his/her cheek to collect cheek cells. This does NOT mean collect salivia ! Gently rub the tooth pick in the center of your slide and leave it in that spot until Ms. Mitchell can place a drop of bromethanol blue stain on the slide. Place a coverslip over the spot of stain slowly from left to right to prevent air bubbles from forming under the cover slip. Place your slide on the stage and view it under low power. Uder your hypothesis, make a section labeled DATA Trace a circle with the cup provided to use as your field of view. Draw the cheek cell. Only draw ONE cell. You will have several in your field of view but only draw one cell. Neatly label the cell membrane, cytoplasm, and nucleus . Use straight lines and label write your words outside the area of the field of view. Under your drawing write the total magnification for the cheek cell under low power. Wet Mount Plant cell: Use your other slide. Use the sample of the potatoe to make a wet mount slide. Follow all the same directions above for the the cheek cell. Also follow all the directions for drawing your field of view and the parts of the plant cell. In addition to the cell membrane , nucleus, and cytoplasm, also label the cell membrane. Only Draw one cell even though you will see many cells. Data questions : Answer the following questions under your drawings of the animal and plant cell. 1. How do the animal and plant cell look different under low power? Describe traits like color, shape, size… 2. How do the animal and plant cell look the same under low power? Describe traits like color, shape, size 3. Why did each cell sample have to be stained? 4. What do you think would happen to the RESOLUTION of each cell if you attempted to view it under high power? Conclusion: After completing the lab , doing your drawings, and answering your data questions, write your conclusion about the original question in 3 sentences.