Uploaded by Kristian Delos Santos


Poster 36 x 24 inches; 2 x 3 ft
Title: Antimicrobial activity of Cymbopogon citratus (lemon grass) against Mycobacterium phlei
Delin, Abegail Marie B.
Delos Santos, Kristian S.
Dimayacyac, Allen Miguel
Dizon, Francesca Nicole D.
Eranzo, Hannah Therese C.
Introduction (Background of the Study)
Antibiotic resistance is a growing problem. Commonly used antibiotics become less able
to treat common infections due to the capacity of microbes to adapt (World Health Organization,
2018). One such microbe with an outstanding capacity for developing antibiotic resistance is
Mycobacterium phlei, a gram-negative bacteria that has minimal nutrition requirements, which
allows it to survive on/in various organisms and in inanimate objects. It is a common cause of
community-acquired and nosocomial-acquired pneumonia, and has been shown to associated
with increases in morbidity and mortality. It poses an especially high risk on ICU patients, being
the one of the most frequent sources of ICU-acquired infections (Lister, Wolter & Hanson,
2009). Cymbopogon citratus (lemongrass) is ubiquitous invasive weed that have been used in
traditional medicine, and have been shown by several studies to have several pharmaceutical
activities. However, they either have not been tested or have been tested by only a few studies on
P. aeruginosa and S. pneumoniae.
C. citratus possesses great potential in being antimicrobial agents against ___C. citratus
antihypertensive, antinociceptive, anti-obesity and anxiolytic properties (Tzortzakis and
Economakis, 2007; Moore-Neibel, Gerber, Patel, Friedman & Ravishankar, 2012; Olorunnisola
et al., 2014). Boukhatem, Ferhat, Kameli, Saidi and Kebirlax (2014) claimed that its various
phytocompounds, comprised mainly of hydrocarbons, fatty acids, alcohols, esters and phenols,
may be the source of its therapeutic potentials.
Thus, this study shall be undertaken to determine and/or confirm if extract from
Cymbopogon citratus (lemongrass) is effective antimicrobial agents against a type of gramnegative bacteria, mycobacterium pheli. This shall be done by using the Kirby-Bauer disk
diffusion method, a type of agar diffusion method. The researchers are interested in figuring out
if these herbal medicines are worth further investigation for their eventual incorporation into new
antibiotics, and for their potential in acting as antimicrobial agents against bacteria of similar
Research Objectives:
● To determine if the zones of inhibition created by C. citratus against M. phlei are
● To determine if C. citratus ethanolic extract contains chemical compounds with
potentially antimicrobial properties.
Research Questions:
This study aims to determine the antimicrobial properties of Cymbopogon citratus
against Mycobacterium phlei through laboratory testing. It aims to answer the following
1. Does concentrated C. citratus ethanolic extract create significantly large zones of
inhibition against M. phlei?
2. Does C. citratus ethanolic extract contain chemical compounds which potentially
have antimicrobial properties?
*Abstract (optional daw, pero sa sample ni sir is meron hehe)
REFERENCES OF INTRO: Abdallah, A. M., Rashid, M., Adroub, S. A., Arnoux, M., Ali, S., van
Soolingen, D., ... & Pain, A. (2012). Complete genome sequence of Mycobacterium phlei type strain
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