Poster 36 x 24 inches; 2 x 3 ft Title: Antimicrobial activity of Cymbopogon citratus (lemon grass) against Mycobacterium phlei Proponents: Delin, Abegail Marie B. Delos Santos, Kristian S. Dimayacyac, Allen Miguel Dizon, Francesca Nicole D. Eranzo, Hannah Therese C. *Abstract Introduction (Background of the Study) Antibiotic resistance is a growing problem. Commonly used antibiotics become less able to treat common infections due to the capacity of microbes to adapt (World Health Organization, 2018). One such microbe with an outstanding capacity for developing antibiotic resistance is Mycobacterium phlei, a gram-negative bacteria that has minimal nutrition requirements, which allows it to survive on/in various organisms and in inanimate objects. It is a common cause of community-acquired and nosocomial-acquired pneumonia, and has been shown to associated with increases in morbidity and mortality. It poses an especially high risk on ICU patients, being the one of the most frequent sources of ICU-acquired infections (Lister, Wolter & Hanson, 2009). Cymbopogon citratus (lemongrass) is ubiquitous invasive weed that have been used in traditional medicine, and have been shown by several studies to have several pharmaceutical activities. However, they either have not been tested or have been tested by only a few studies on P. aeruginosa and S. pneumoniae. C. citratus possesses great potential in being antimicrobial agents against ___C. citratus has been shown to have bactericidal, fungicidal, antioxidant, anti-inflammatory, antihypertensive, antinociceptive, anti-obesity and anxiolytic properties (Tzortzakis and Economakis, 2007; Moore-Neibel, Gerber, Patel, Friedman & Ravishankar, 2012; Olorunnisola et al., 2014). Boukhatem, Ferhat, Kameli, Saidi and Kebirlax (2014) claimed that its various phytocompounds, comprised mainly of hydrocarbons, fatty acids, alcohols, esters and phenols, may be the source of its therapeutic potentials. Thus, this study shall be undertaken to determine and/or confirm if extract from Cymbopogon citratus (lemongrass) is effective antimicrobial agents against a type of gramnegative bacteria, mycobacterium pheli. This shall be done by using the Kirby-Bauer disk diffusion method, a type of agar diffusion method. The researchers are interested in figuring out if these herbal medicines are worth further investigation for their eventual incorporation into new antibiotics, and for their potential in acting as antimicrobial agents against bacteria of similar physiology. Research Objectives: ● To determine if the zones of inhibition created by C. citratus against M. phlei are significant ● To determine if C. citratus ethanolic extract contains chemical compounds with potentially antimicrobial properties. Research Questions: This study aims to determine the antimicrobial properties of Cymbopogon citratus against Mycobacterium phlei through laboratory testing. It aims to answer the following inquiries: 1. Does concentrated C. citratus ethanolic extract create significantly large zones of inhibition against M. phlei? 2. Does C. citratus ethanolic extract contain chemical compounds which potentially have antimicrobial properties? Methodology: Results Conclusion Recommendation *Abstract (optional daw, pero sa sample ni sir is meron hehe) References REFERENCES OF INTRO: Abdallah, A. M., Rashid, M., Adroub, S. A., Arnoux, M., Ali, S., van Soolingen, D., ... & Pain, A. (2012). Complete genome sequence of Mycobacterium phlei type strain RIVM601174.