iWorx Cardiovascular Research Solutions iWorx offers complete systems to study physiological function for a variety of scientific research applications. Our cardiovascular products are used to assess cardiac performance and hemodynamics both in vivo and in vitro. We offer transducers, accessories, amplifiers, data recorders and software for in situ experiments, isolated heart preparations and isolated tissue protocols. Applications Blood pressure ECG Blood flow and cardiac output Pressure-Volume Sonomicrometry dimension and volume Isolated Langendorff and working heart models Isolated tissue studies Data Acquisition and Analysis for the Life Sciences LabScribe2 Software LabScribe2 software is a powerful recording and analysis software package offered by iWorx. LabScribe2 has an intuitive, user-friendly interface for setting up acquisition screens, calibrating signals and analyzing data. Up to 128 channels of data can be displayed simultaneously at sampling rates as high as 100,000 samples/second. A comprehensive set of analysis routines have been preconfigured making data analysis and interpretation quick and easy. Specific analysis modules for cardiovascular research are available to analyze pressure signals, blood flow data, ECG recordings, ventricular pressure-volume loops and sonomicrometry dimension data. LabScribe2 interface showing data being acquired in real-time. LabScribe2 includes a scripting function for use if the built-in analysis tools fall short of what is required for your application. Scripting languages currently supported include: Dadisp, Python, MatLab, LabView, and C++. Features Display LabScribe2 software displays data through a customizable, user-friendly, and intuitive interface. The number of input and computed channels, the display time, and scaling of the X and Y axes are just a few of the parameters that can be saved to a viewing template. Simply click one button to change from one view to another. Changing how data is displayed can easily be accomplished by setting up different views. Real-time Online Calculations Monitor and observe functional parameters during an experiment with an array of real-time calculations and user-defined computed channels. Labscribe2 software can calculate rates, min/max/mean values, systolic/diastolic values as well as derivatives and integrals in real-time. A unique channel math function allows users to create their own formulae for viewing signals in real-time. Data Reduction Data can be analyzed and reduced in the Analysis window. Selections of data can be averaged and written to the LabScribe2 Journal or directly to a text file. For example, the software can be instructed to break a file up into five-minute pieces, determine the average heart rate for the data in each segment, and write those values to a report. Data can be saved for several hours in one file. Marks can be entered to easily navigate to specific time points for data viewing and reduction. LabScribe2 Software Real-Time Annotations Mark data during an experiment with free-form text notes. These marks are searchable and appear in the field of data to which they apply. Marks are time locked to the record and can be used to “Go To” any marked region of the data. Marks can be preset and assigned as keyboard shortcuts for quick and easy annotation. Advanced Analysis Specific time points and features of a signal can be AutoMarked for further analysis and validation. For example, the ECG AutoMark feature will place marks at the Q, R, S, T, and P waves. The blood pressure AutoMark will place marks at systolic and diastolic points, dichrotic notch and max/min dP/dt. Data from Automarks are sent to the journal from where they can be exported easily to Excel or another analytical program for further analysis. XY Window Any two channels can be displayed in XY format, online or offline. LabScribe2 software supports area measurements and segment slopes from such plots. And, of course, you can save the plots as pictures or text. Settings files Settings files can be created for different experimental protocols. The number of input channels, calibration parameters, graph settings, computed channels, views and analysis protocols are all saved to a settings file. FFT analysis LabScribe2 includes a Fast Fourier Transform feature for frequency analysis of any signal. Complex mathematical calculations are available for analyzing any cardiac signal. Examples include some of the pressure-volume loop algorithms included with LabScribe2. Input/Output LabScribe2 supports various pacing and stimulation protocols with a built-in stimulator interface which allows delivery of a single, continuous, or train of pulses. Pulse durations, frequencies and amplitudes can be changed as the protocol is being delivered. The software also offers full control of digital I/O lines that can be used to count events, or control devices in the lab environment. Export All recorded data can be exported to either a text file or a picture (.png, .jpg) making reports or posters much easier to create. Of course, you can always print your data from any window at any scale. Import An optional import module is available so that data acquired from other acquisition systems can be analyzed with LabScribe's powerful analysis routines. FFT analysis can be performed on any signals. For example, heart rate variability can be calculated using either ECG or Blood pressure from this menu. Blood Pressure Measurement Proper assessment of cardiac function and hemodynamic performance requires a measurement of pressure from within the heart and the peripheral arteries. iWorx offers several solutions for measuring pressure including fluid-filled pressure transducers, catheter-tipped micro pressure sensors and non-invasive tail cuff pressure systems. Systems and Components Fluid-Filled Catheter Transducer The BP-100 blood pressure transducer is an economical and accurate device used to measure direct arterial or venous pressure in animals using the fluid-filled transducer technique. A precision laser-trimmed chip provides accurate and linear measurements over a broad range of pressures. Pressure changes in the vessel are transmitted within the fluid-filled tube to the pressure transducer and acquired for monitoring and further analysis. Tubing is available as small as 1 mm in outside diameter making this technique well-suited for measuring pressures in very small vessels. Catheter-Tipped Transducers iWorx offers catheter-tipped solid-state pressure catheters in sizes ranging from 1.2F to 1.6F. These catheters are recommended for measuring pressure in small vessels as well as ventricular pressures in small rodents. Solid-state transducer-tipped catheters offer superior sensitivity and temporal resolution compared to fluid-filled catheters and are generally more suitable for measuring intra-cardiac pressures from animal species with high intrinsic heart rates. BP-100 and BP-100RE Intravascular Blood Pressure Transducers measure direct arterial or venous pressure in animals. FTM-1211B-0018 1.2F Mouse Pressure Catheter FTM-1611B-0018 1.6F Rat Pressure Catheter. Non-invasive Blood Pressure Invasive measures of pressure are not always possible and, as a result, there is a need for non-invasive methods to detect pressure. The most widely accepted technique for animals is the tail-cuff method. iWorx offers the IITC noninvasive blood pressure systems for mice and rats. The IITC Life Science amplifier with built-in inflation outputs systolic pressure, mean pressure, diastolic pressure and heart rate as analog signals which can be acquired by iWorx data recorders. Systems include the amplifier with built-in cuff pump, sensor and restrainer, cables, sphygmomanometer and accessories. Warming chambers are available so that no heating pads are required. IITC-229M Noninvasive Blood Pressure System Single Mouse and IITC-229R Noninvasive Blood Pressure System – Single Rat. Blood Pressure Analysis Arterial pressure calculations: Heart Rate (HR) Maximum Pressure (Pmax) Minimum Pressure (Pmin) Mean Arterial Pressure (MAP) Systolic and diastolic pressure Maximum dP/dt (dP/dtmax) Minimum dP/dt (dP/dtmin) Pulse Height Notch Pressure Systolic Duration Diastolic Duration Cycle Duration Time To Peak Election Time Percent Recovery Ventricular pressure calculations: Heart Rate (HR) Maximum Pressure (Pmax) Minimum Pressure (Pmin) Mean Pressure (Pmean) End-systolic Pressure (Pes) End-diastolic Pressure (Ped) Maximum dP/dt (dP/dtmax) Minimum dP/dt (dP/dtmin) Developed Pressure dP/dt@ Contractility Index Relaxation Index Systolic Duration Diastolic Duration Cycle Duration Tension Time Index Relaxation Time Isovolumetric Time Tau (Weiss, Logistic, Glantz, Mirsky) Acquire and analyze data from blood pressure transducers. Data can be analyzed from ventricular and arterial signals in real-time. The blood pressure module automatically calculates common indices of function from the blood pressure signal where it can easily be exported to another program for further analysis. Electrocardiograms (ECG/EKG) The cardiac cycle involves a sequential contraction of the atria and ventricles which is triggered by the coordinated electrical activity of the myocardial cells in the heart. These electrical currents can be recorded non-invasively through electrodes producing what is known as an electrocardiogram or ECG. The ECG can be measured in 3 dimensions by looking at the electrical signals from different views or leads. iWorx offers complete systems for humans and animals to study ECG in some of the most common lead configurations - 3 lead ECG, 6 lead ECG, 12 lead ECG. ECG calculations: Record and AutoMark the ECG, sending the calculations to the journal from where they can be exported easily to Excel or other analytical program for further analysis. Heart rate Time at R R-R Interval Delta R-R PR Interval P Duration QRS Duration QT Interval QTc Interval ST Segment T Duration T-P Duration Heart Rate Variability (HRV*) calculations: Delta R-R Low Power High Power Mean Power at low band Mean Power at high band *Note: HRV can be derived from either an ECG trace or a blood pressure trace. Electrodes C-ISO-255 3-Lead Isolated wide band width cable for ETH-256C Amplifier C-ISO-104 3-Lead Isolated ECG Amplifier Cable A-NE-300 Set of 3 Needle Electrodes with Safety Pin Connectors A-NE-500 Set of 5 Needle Electrodes with Safety Pin Connectors C-WT-100 The Wilson Terminal Cable allows simultaneous recording of periodical leads and one chest lead when used with iWorx 214 Recording Interface and a C-ISO-104 3-Lead Isolated ECG Amplifier Cable. Amplifier/Signal Conditioner Options: ETH-256C Two Channel Combination Bridge/ECG/EMG/EEG Amplifier SAE-100 Small Animal ECG Amplifier SAE-200 Small Animal ECG Amplifier Data Recording Options: IX-300 series IX-400 series Intracardiac Electrical Potentials iWorx offers minimally invasive 8-electrode catheters for studying intracardiac electrical potentials in the rodent. Intracardiac electrophysiology catheters allow for in depth analysis of biopotential propagation through the heart. Up to 4 regional intracardiac electrograms can be recorded simultaneously. The same catheter can be used to pace the heart while recording electrical activity. Applications His Bundle characterization SA node characterization General signal propagation Endocardial ECG assessment Regional atrial assessment Conduction studies Qualification of cardiac insult Intracardiac electrical potentials look similar to a surface ECG. Multiple signals from different locations can be recorded simultaneously and analyzed from a single catheter. Amplifier/Signal Conditioner Options: ETH-256C Two Channel Combination Bridge/ECG/EMG/EEG Amplifier SAE-100 Small Animal ECG Amplifier SAE-200 Small Animal ECG Amplifier Data Recording Options: IX-300 series IX-400 series Sensors/Electrodes: A-NE-100 Subdermal Ground Electrode System Components: FTS-1113A-0518 1.2F Cardiac Electrogram Catheter FTS-1913A-1018 1.9F Cardiac Electrogram Catheter FI893B Catheter Interface Box (includes extension cable) FI893B Catheter Interface Box with FTS-113A-0518 Catheter. Pressure Volume Loops Pressure-volume loops have become the gold standard for studying myocardial contractility, compliance, muscle energetic and other quantitative measures of cardiac function. iWorx offers a comprehensive acquisition and analysis solution for PV loop experiments. Our LabScribe2 PV loop module can be used to analyze PV data from any source including conductance catheters, admittance catheters or sonomicrometry dimension crystals. Elastance and compliance curves can be generated in real-time from within the acquisition interface providing instant feedback on the quality of the occlusions being performed. Unlike other software offerings for PV analysis, all the acquisition and analysis takes place within one program providing seamless and time saving options for generating data for publication. PV Loop Calculations: Maximum and Minimum Volume Diastolic and Systolic Volume Stroke Volume Cardiac Output Ejection Fraction End-Systolic Pressure Volume Relationship (ESPVR) End-Diastolic Pressure Volume Relationship (EDPVR) Preload-Recruitable Stroke Work (PRSW) Pressure-Volume Area (PVA) Arterial Elastance Time-Varying Elastance Maximum Power Preload-Adjusted Maximal Power Potential Energy Cardiac Efficiency Heart Rate Variability (HRV) Calculations Plotting: PV Loops PRSW Max dp/dt vs EDV PVA vs EDV PVA vs ESP Typical screen configuration for acquiring PV data in the rodent. Signals being displayed are LVP, LVV, Phase, Magnitude and dP/dt as well as an XY plot depicting LVP vs. LVV. A PV Loop AutoMark feature can be used to display key delineators and write data to the online journal in realtime. Complete Systems iWorx offers the Scisense ADVantage system for rodents which yields the most accurate, reliable and repeatable measurements of pressure-volume data. Scisense catheters range in size from 1.2F to 1.6 F and can acquire both pressure and volume data from a single catheter. Typical applications include heart failure studies, gene therapy and manipulation protocols, and pharmacology studies. Benefits of the ADVantage system include: No Saline bolus required Real-time measurement of parallel conductance Absolute ventricular volumes Lower standard deviations Accurate, reliable, and reproducible data Data Recording Options: IX-300 series IX-400 series Scisense FV897B Pressure-Volume System Components FV897B ADVantage PV System FTS-1212B-4518 1.2F mouse PV catheter FTS-1912B-8018 1.9F rat PV catheter FC-ADV-PK Cable package FM-1287-1 mouse myocardial probe FM-1987-1 rat myocardial probe HI76301ADV Conductivity probe LS20/PVL software module Isolated Heart Preparations The isolated perfused heart system, as originated by Oscar Langendorff more than a century ago, has become a mainstay of pharmacological and physiological research. The system allows the examination of cardiac inotropic (cardiac contractile strength), chronotropic (heart rate), and vascular effects without the complications of an intact animal model. The original design has evolved to encompass both constant pressure and constant flow models in recirculating or non-recirculating modes, as well as working heart models. iWorx provides transducers, accessories and data acquisition hardware and software to be used in any of these configurations. Record and AutoMark LDEVP, systolic and diastolic from a blood pressure curve, sending the calculations to the journal and a spreadsheet file in real-time. Modes Langendorff: Constant pressure recirculating Constant pressure nonrecirculating Constant volume recirculating Constant volume non-recirculating Working Heart Pacing For pacing the heart during a Langendorff preparation, the built-in digital stimulator of an iWorx data acquisition unit can be used. iWorx stimulators have very flexible controls, which even allow real-time changes in stimuli parameters to be made (with the touch of a button) through LabScribe2 software. Force, Pressures, Flow, and Volumes Contractile forces, pressures, flow, and volumes can be measured using any combination of recording electrodes, strain-gage transducers and amplifiers which are available from iWorx. K+, Ca2+, Cl- and pH can also be measured directly from the perfusate by using electrodes connected to an iWorx signal conditioner. O2 consumption can be measured by placing an O2 electrode in the inflow and in the effluent and calculating the difference in concentration. ECG Electrocardiograms can also be recorded from the isolated heart using electrodes connected to any of our low noise biopotential amplifiers. Transducers BP-100 Blood Pressure Transducer FT-302 Force Transducer ISE-100 pH Electrode DO2E-200 Oxygen Electrode/various ion electrodes TM-100 Temperature Electrode Amplifier/Signal Conditioner Options: ETH-256C Two Channel Combination Bridge/ECG/EMG/EEG Amplifier SAE-100 Small Animal ECG Amplifier SAE-200 Small Animal ECG Amplifier ETH-401 4 Channel Transducer Interface Data Recording Options: IX-300 series IX-400 series Recorders iWorx offers rugged, high speed, high resolution data acquisition systems suitable for teaching and research applications. iWorx recorders include easy-to-use LabScribe2 data acquisition software in both a teaching and research version. LabScribe2 for Teaching is easy-to-use and includes courseware for more than 125 exercises in cardiovascular, respiratory and neuromuscular physiology. LabScribe2 for Research allows advanced analysis capabilities as well as scripting. All recorders are protected by a three-year warranty. iWorx 300 Series Research Grade Recorders iWorx 400 Series Recorders The iWorx 300 Series Research Grade Recorders come in 4, 8 and 16 channel configurations. These systems provide the high resolution and sensitivity required for 300 Series Recorders cardiovascular and hemodynamic research as well as organ and tissue bath studies, Oocyte clamp and epithelial voltage clamp applications. All 300 series recorders include stimulator outputs for each input channel, LabScribe2 Data Acquisition and Analysis Software for Windows and Macintosh computers, USB Cable and manuals. The recorders are available with or without galvanic isolation. iWorx 400 series are economical, high performance 4, 8 and 16 channel data recorders for use with a broad range of amplifiers. The recorders offer 16-bit resolution at maximum data collection speeds up to 10 KHz, making them appropriate for most research 400 Series Recorders applications. All 400 series recorders include LabScribe2 Data Acquisition and Analysis Software for Windows and Macintosh computers, USB Cable and manuals. The 408 and 416 recorders include a low voltage stimulator output. Input Number of Inputs 4, 8 or 16, with or without galvanic isolation Input Range + 10 VDC Stimulator Outputs Number 4, 8 or 16 Resolution 16 Bit Output Range + 10 VDC Modes Pulse, Train, Constant, Step, Ramp,Triangle A/D Converter Sample Speed Interface Power Input Analog Inputs Input Range Stimulator Outputs Number 1 Resolution 16 Bit Output Range + 10 VDC Modes Pulse, Train, Constant, Step, Ramp, Triangle A/D Converter Sample Speed up to 5 kHz USB 1.1 / 2.0 full speed 120 V/220 V, 50/60 Hz iWorx 300 Series Part Numbers IX-304Ti (isolated with transducer amplifier) IX-304T (with transducer amplifier) IX-308Ti (isolated with transducer amplifier) IX-308T (with transducer amplifier) IX-316Ti (isolated with transducer amplifier) IX-316T (with transducer amplifier) 4, 8 or 16 + 10 VDC Interface Power 10 kHz simultaneously (404) 100 kHz aggregate (408, 416) USB 1.1 / 2.0 full speed USB iWorx 400 Series Part Numbers IX-404 IX-408 IX-416 Recorders iWorx 228S Recorder IX-228 Recorder The iWorx 228S Recorder has 10 input channels and both a low voltage and a high voltage stimulator output. Channels 1 and 2 can be configured for isolated recording of biopotentials or as DC coupled inputs. Channels 3 through 10 each include a transducer amplifier. These recorders exhibit the high resolution and low noise required for small animal cardiovascular research, Oocyte clamp and high end metabolic research applications. The IX-228S Recorder includes LabScribe2 Data Acquisition and Analysis Software for Windows and Macintosh computers, USB Cable and manuals. Part Number IX-228S Transducer Inputs Number of Transducer Inputs: Input Range: Resolution: Isolation: Excitation: Connector: Gain: 8 + 10 VDC 16 Bit No + 5 VCD, 100 mA DIN Programmable with Input Resistor Biopotential Inputs Number of Biopotental Inputs: Input Range: Resolution: High Pass Filters: Low Pass Filters: Isolation: Connector: 2 Software Configurable 16 Bit Software Configurable Software Configurable 3 kV AAMI Safety High Voltage Stimulator Output Number: Resolution: Connectors: Output Range: Compliance: Max. ON Time: 1 12 bit HV Safety 0 - 20 mA 150 VDC 10 msec. Low Voltage Stimulator Output Number: Resolution: Connectors: Output Range: Modes: 1 16 bit BNC + 10 VDC Pulse, Train, Constant, Step, Ramp, Triangle A/D Converter Sample Speed: 100 kHz Aggregate Interface USB: 1.1/2.0 Full Speed Power: 5 VDC 2.6A Enclosure: Plastic Warranty: Protected by 3 year warranty Software: LabScribe2 Recording and Analysis Software Components Amplifier/Signal Conditioner Options Complementary Products ETH-256C Two Channel Combination Bridge/ECG/EMG/EEG Amplifier A high performance, generalpurpose research amplifier with high input impedance and high CMR to allow reliable low noise recording of biopotential (ECG,EMG,EEG) signals, as well as outputs from strain gauge type transducers, such ETH-256C as force, displacement and pressure. Pulse Oximeter for Animals PO2-300D Pulse Oximeter measures blood oxygen saturation (SpO2) in animals using a lingual or animal wrap sensor. Sensors are sold separately. ETH-401 A dedicated bridge amplifier for iWorx-brand transducers that provides four channels of excitation power, gain, and noise filtration. It exhibits low system noise and offset, making it suitable for use with high resolution data acquisition systems. ETH-401 SAE-100 and SAE-200 Small Animal ECG Amplifiers 3-lead signal conditioner optimized for high quality, low noise recording of small animal (rat and mouse) ECG. The SAE-100 has a DIN SAE-200 connector and is powered by the 300 series recorder. The SAE-200 has a BNC connector and includes an external power supply. Sensors: PO2-200-AWC Animal Wrap Sensor PO2-200-ALC Animal Lingual Clip Sensor Animal Respiration Monitor RM-204 (Animal) Respiration Monitor with BNC connector employs piezo technology to follow the relative depth and frequency of breathing. The measuring element is mounted in a belt that straps around the chest of the subject. PO2-300D RM-204 Temperature Sensor The TM-100 is a self-contained temperature transducer with a range of 0o C to 50o C. The sensor is a rugged epoxy-sealed pellet measuring only 3.0 mm in length and 1.0mm in diameter. The small mass of the sensor gives the TM100 a fast response time. The TM-100 sealed enclosure makes cleaning and/or sterilization easy. The TM-100 requires a transducer conditioning amplifier such as the ETH-256 or ETH-401 or an iWorx computer interface equipped with transducer inputs. Cardiovascular Research Systems Tissue Bath Systems iWorx offers two, four, and eight channel tissue bath systems. Systems are provided with stands, tissue tensioners, heated circulating pumps, and everything you need to set up an organ bath. The chambers are available in 5, 10, 25, and 50 ml sizes. The iWorx Web-site allows you to customize the system that best meets your needs by selecting the appropriate tensioner, tissue bath, tissue holder, data acquisition system, transducer, stimulator, and heating circulator. Myograph Systems iWorx offers offers 4-channel (M4) or single-channel (M1) Myograph systems from Radnoti Glass Technology. These systems are designed for researchers performing contractile force studies on small ring samples with sizes ranging from 60 µm to over 1 mm in diameter. Examples include mouse aortic rings and small intestinal ring samples as well as micro-vessel preparations like mesenteric arteries. The Myograph systems include myograph chambers with transducers and amplifiers, a base with sliding wrist rest, temperature controllers, stands, tubing kits and all other essential items to conduct an experiment. Blood Flow Systems iWorx offers a range of blood flow meters and probes through a distribution arrangement with Transonic Systems. Transonic Systems’ transit time technology has become the gold-standard in volume flow measurements. Probes range in size from several centimeters in diameter to less than 1 mm in diameter and can be used to accurately measure flow in large animals (renal arteries, aortic flow, coronary flow) as well in small animals (cardiac output in mice and rats). Flow data can be acquired and analyzed in LabScribe2 providing measurements of stroke volume, heart rate, flow rate, mean flow, frequency, derivatives and integrals. iWorx offers a myograph normalization module (LS-20NM) which calculates the optimal pretension settings for each sample prior to conducting an experiment. Want to Learn More? Contact us by email at info@iworx.com or by phone at (800) 234-1757. iWorx Systems, Inc. One Washington Street, Suite 404 Dover, New Hampshire 03820 (T) 800-234-1757 / 603-742-2492 (F) 603-742-2455 info@iworx.com www.iworx.com Data Acquisition for the Life Sciences LabScribe is a trademark and iWorx is a registered trademark of iWorx Systems, Inc. ©2009 iWorx Systems, Inc.