University of Iowa Iowa Research Online Theses and Dissertations Spring 2013 Internal and marginal fit Of pressed and cad lithium disilicate crowns made from digital and conventional impressions Evanthia Anadioti University of Iowa Copyright 2013 Evanthia Anadioti This dissertation is available at Iowa Research Online: http://ir.uiowa.edu/etd/2435 Recommended Citation Anadioti, Evanthia. "Internal and marginal fit Of pressed and cad lithium disilicate crowns made from digital and conventional impressions." MS (Master of Science) thesis, University of Iowa, 2013. http://ir.uiowa.edu/etd/2435. Follow this and additional works at: http://ir.uiowa.edu/etd Part of the Oral Biology and Oral Pathology Commons INTERNAL AND MARGINAL FIT OF PRESSED AND CAD LITHIUM DISILICATE CROWNS MADE FROM DIGITAL AND CONVENTIONAL IMPRESSIONS by Evanthia Anadioti A thesis submitted in partial fulfillment of the requirements for the Master of Science degree in Oral Science in the Graduate College of The University of Iowa May 2013 Thesis Supervisor: Professor Steven A. Aquilino Copyright by EVANTHIA ANADIOTI 2013 All Rights Reserved Graduate College The University of Iowa Iowa City, Iowa CERTIFICATE OF APPROVAL _______________________ MASTER’S THESIS _______________ This is to certify that the Master’s thesis of Evanthia Anadioti has been approved by the Examining Committee for the thesis requirement for the Master of Science degree in Oral Science at the May 2013 graduation. Thesis Committee: __________________________________ Steven A. Aquilino Thesis Supervisor __________________________________ David G. Gratton __________________________________ Julie A. Holloway __________________________________ Isabelle L. Denry __________________________________ Geb W. Thomas To my family, your love and support made it all worthwhile. ii ACKNOWLEDGMENTS First and foremost I wish to express my sincere appreciation and gratitude to Dr. Steven A. Aquilino, my thesis supervisor, for his phenomenal knowledge, invaluable guidance, patience during the frustrating times and commitment to the highest standards in every step of this research project. I would, also, like to thank my thesis committee, Drs. David G. Gratton, Julie A. Holloway, Isabelle L. Denry and Geb W. Thomas, for their help, time, encouragement and recommendations throughout the last three years. I, also, wish to thank Dr. Fang Qian, for the statistical analysis and Dr. Marcos Vargas for his help with the E4D system. Special thanks to Mr. R Henry Husemann for his time, skills and help with the pressing procedure and to Mr. Josh Kistner, from the Geomagic technical support, for his generous time, support and knowledge in developing a protocol for the measuremnets. I would like to acknlowledge the University of Iowa for providing me the facilities to complete this project. I am grateful for the financial support received from the Academy of Esthetic Denstirty and the Greater New York Academy of Prosthodontics, as well as for the generous donation of their porducts from Ivoclar Vivadent and Whip Mix Corp. iii TABLE OF CONTENTS LIST OF TABLES ............................................................................................................. vi LIST OF FIGURES .......................................................................................................... vii CHAPTER I. INTRODUCTION ........................................................................................... 1 Research Objectives......................................................................................... 3 Research Hypotheses ....................................................................................... 4 II. LITERATURE REVIEW ................................................................................ 5 Background of full-coverage restorations ....................................................... 5 Materials used for full-coverage dental restorations ....................................... 5 All-ceramic ............................................................................................... 6 IPS e.max all-ceramic ............................................................................... 8 Longevity of all-ceramic restorations ............................................................ 13 Marginal and internal fit of dental restorations ............................................. 15 Methods to measure the marginal gap ........................................................... 18 Marginal adaptation of all-ceramics .............................................................. 27 Dental impressions......................................................................................... 30 Dental plaster and stone ................................................................................. 35 Computer-Aided Design/Computer-Aided Manufacturing (CAD/CAM) systems ........................................................................................................... 37 Stereolithography (SLA) ............................................................................... 43 Digital dental impressions ............................................................................. 45 Lava™ C.O.S. ........................................................................................ 48 Accuracy of Lava™ C.O.S. .................................................................... 55 Repeatability of Lava™ C.O.S. .............................................................. 56 Fit of crowns made from Lava™ C.O.S. impressions............................ 57 E4D Dentist System™............................................................................ 59 Accuracy of E4D Dentist System™ ....................................................... 63 Summary-Purpose.......................................................................................... 64 III. MATERIALS AND METHODS .................................................................. 65 Introduction.................................................................................................... 65 Pilot study ...................................................................................................... 65 Results of the pilot study ........................................................................ 68 Validation of the Digital Measurement Technique ................................ 70 Definitive study ............................................................................................. 72 Study design................................................................................................... 73 Master die fabrication ............................................................................. 73 Impression making ................................................................................. 74 Conventional impression ........................................................................ 74 Digital impression .................................................................................. 77 IPS e.max Press crown fabrication ......................................................... 78 IPS e.max CAD crown fabrication ......................................................... 81 Measurement of the crown fit ........................................................................ 84 iv Statistical analysis .......................................................................................... 91 IV. RESULTS ...................................................................................................... 93 3D results ....................................................................................................... 93 One-way ANOVA for 3D Marginal fit .................................................. 93 Two-way ANOVA for 3D Marginal fit.................................................. 95 2D results ....................................................................................................... 96 One-way ANOVA for 2D Marginal fit .................................................. 96 Two-way ANOVA for 2D Marginal fit.................................................. 97 One-way ANOVA for 2D Internal fit .................................................... 99 Two-way ANOVA for 2D Internal fit .................................................. 100 V. DISCUSSION .............................................................................................. 103 Study design................................................................................................. 111 Possible limitations of the study .................................................................. 116 Avenues for future research ......................................................................... 117 Clinical significance .................................................................................... 117 VI. CONCLUSIONS ......................................................................................... 118 APPENDIX A. RAW DATA ........................................................................................ 120 APPENDIX B. GEOMAGIC IMAGES ........................................................................ 124 REFERENCES ............................................................................................................... 132 v LIST OF TABLES Table 1. Properties of contemporary all-ceramic materials ................................................... 12 2. Current digital impression systems .......................................................................... 47 3. Comparisons of the ten variables among the four Experimental Groups: Pilot data ........................................................................................................................... 69 4. Pressing program parameters for IPS e.max Press LT ............................................ 80 5. Speed crystallization/Glaze LT program parameters............................................... 83 6. Mean 3D marginal gap in mm of 4 experimental groups ........................................ 94 7. Result of One-Way ANOVA for 2D marginal fit.................................................... 96 8. Result of Two-Way ANOVA for 2D marginal fit ................................................... 97 9. Pairwise comparisons of mean 2D marginal gap by experimental group ............... 98 10. Result of One-Way ANOVA for 2D internal fit.................................................... 100 11. Result of Two-Way ANOVA for 2D internal fit ................................................... 100 12. Pairwise comparisons of mean 2D internal gap by experimental group ............... 102 A1. Average marginal gap for 3D measurements ........................................................ 120 A2. Average marginal and internal gaps for 2D measurements ................................... 124 vi LIST OF FIGURES Figure 1. Laser Triangulation. ................................................................................................. 25 2. The Lava™ C.O.S. system ...................................................................................... 48 3. The rotation of the aperture mechanism .................................................................. 50 4. In-plane object coordinates ...................................................................................... 51 5. The depth information ............................................................................................. 51 6. The Active Waveform Sampling. ............................................................................ 52 7. The E4D Dentist System™...................................................................................... 60 8. Study Groups for the pilot study .............................................................................. 66 9. Study Groups for the definitive study. ..................................................................... 72 10. Master die frontal view ............................................................................................ 73 11. Master die occlusal view.......................................................................................... 74 12. Stone die .................................................................................................................. 77 13. Resin die .................................................................................................................. 78 14. Intaglio of one indicative crown from each experimental group ............................. 84 15. Defined area around the margin of the Master Die with 0.75mm occlusalgingival width .......................................................................................................... 86 16. Grooves on the base of the Master Die in order to standardize the sections ........... 87 17. Facial-lingual section with the 7 standardized points .............................................. 88 18. Mesial-distal section with the 7 standardized points ............................................... 88 19. 3D measurement of the predefined area .................................................................. 93 20. Mean 3D marginal gap of 4 experimental groups ................................................... 95 21. Mean 2D marginal gap in mm ................................................................................. 99 22. Mean 2D internal gap in mm ................................................................................. 102 B1. Group A (PVS/press) 3D colored map .................................................................. 124 B2. Group A (PVS/press) Facial-Lingual section for 2D measurements ..................... 125 vii B3. Group A (PVS/press) Mesial-Distal section for 2D measurements ...................... 125 B4. Group B (PVS/CAD) 3D colored map .................................................................. 126 B5. Group B (PVS/CAD) Facial-Lingual section for 2D measurements ..................... 127 B6. Group B (PVS/CAD) Mesial-Distal section for 2D measurements ...................... 127 B7. Group C (Lava/press) 3D colored map .................................................................. 128 B8. Group C (Lava/press) Facial-Lingual section for 2D measurements .................... 129 B9. Group C (Lava/press) Mesial-Distal section for 2D measurements ...................... 129 B10. Group D (Lava/CAD) 3D colored map ................................................................. 130 B11. Group D (Lava/CAD) Facial-Lingual section for 2D measurements .................... 131 B12. Group D (Lava/CAD) Mesial-Distal section for 2D measurements...................... 131 viii 1 CHAPTER I INTRODUCTION Although marginal opening alone does not directly correlate with microleakage, the accuracy of marginal fit is valued as one of the most important criteria for the clinical quality and success of prosthetic restorations (1). The importance of precise marginal adaptation and the subsequent implications of marginal discrepancies, including microleakage, caries and periodontal inflammation, have been emphasized in many studies (2-7). In addition to marginal fit, internal fit and accuracy play a significant role in the longevity of a full-coverage restoration as well (8-17). Several definitions for marginal deficiencies have been proposed, such as internal gap, marginal gap, horizontal marginal discrepancy, over-extended margin, seating discrepancy and others (18). In addition, several methods, both destructive and non-destructive, to measure marginal and internal fit have been discussed in the literature; including sectioning the crowns, replica technique, profilometry, SEM, image analysis and 3D scanning (19-39). All the required steps during the fabrication of a crown necessitate precision and exactness in order to produce an accurately fitting restoration. Recent advances in technology have dramatically altered impression and crown fabrication procedures; specifically, digital impressions and computer-aided design/computer-aided manufacturing (CAD/CAM) systems have been introduced in dental clinical practice. The impression of the hard and soft tissue of the oral cavity is one of the most crucial steps for a successful dental restoration. The dental impression can strongly affect the fit and accuracy of the indirect restoration. Over the past several decades, the 2 impression materials have changed and today with proper selection and manipulation, excellent impressions can be obtained (40-47). In addition to the impression material, the choice of the impression technique is of great importance (48-51). The combination of the proper material, the most reliable technique and adequate understanding and knowledge by the operator gives the most accurate results (52, 53). The introduction of dental digital impressions is a breakthrough in our specialty (54-57). Digital or virtual impression systems have the potential to produce accurate results while simplifying the entire prosthesis fabrication process, since several laboratory steps are eliminated (5861). Nevertheless, the recent introduction, the limited use and the very limited research with conflicting results do not allow for valid conclusions with regard to the accuracy and reliability of digital impression systems (62-64). Most digital impression systems are designed to be used in conjunction with allceramic restorations. This has coincided with the growing demand for all-ceramic restorations that most closely mimic tooth appearance and the development of all-ceramic materials with strength comparable to metal-ceramic restorations (65-68). Lithium disilicate (IPS e.max, Ivoclar Vivadent) is a glass-ceramic, which claims to combine high flexural strength along with optimum esthetics. It can be processed using either lost-wax hot pressing techniques or CAD/CAM milling procedures. Apart from the properties of the all-ceramic material itself, a very important consideration with regard to the longterm success of the restoration is its internal and marginal fit. Pressed ceramics have been shown to demonstrate marginal accuracy comparable to that of metal restorations (69). On the other hand, the marginal accuracy of milled ceramic restoration is mainly depended on the CAD/CAM system used (70, 71). Studies have shown the latter 3 restorations had inferior marginal fit compared to pressed restorations (72, 73). However, several advancements have occurred in milling technologies and new systems have been introduced that claim to produce accurately fitting restorations (74). There are no studies evaluating the influence of both the impression technique and the prosthesis fabrication technique on the overall fit of the complete final all-ceramic restoration. Research Objectives The purpose of this study was to evaluate in vitro the marginal and internal fit of allceramic crowns made from two different impression techniques (digital vs. conventional) and two different fabrication methods (CAD vs. press). The objectives of this study were: 1. To evaluate in vitro the marginal and internal fit of all-ceramic crowns made from two different impression techniques (digital vs. conventional). 2. To evaluate in vitro the marginal and internal fit of all-ceramic crowns made from two different fabrication methods (CAD vs. press). 3. To determine if there is an interaction between the impression and crown fabrication techniques with regard to the accuracy of the final crown. 4. To determine which combination of impression and crown fabrication technique results in the most accurately fitting crown. 4 Research Hypotheses HO (1) There is no difference in accuracy between the two impression techniques, conventional and digital, considering the marginal and internal fit of the crowns fabricated by those methods. HA (1) There is a difference in accuracy between the two impression techniques, conventional and digital, considering the marginal and internal fit of the crowns fabricated by those methods. HO (2) There is no difference in accuracy between the crown fabrication methods, press and CAD, considering the marginal and internal fit of the crowns fabricated by those methods. HA (2) There is a difference in accuracy between the crown fabrication methods, press and CAD, considering the marginal and internal fit of the crowns fabricated by those methods. HO (3) There is no interaction between the impression technique (conventional and digital) and the crown fabrication method, considering the marginal and internal fit of the crowns fabricated by any combination of those methods. HA (3) There is an interaction between the impression technique (conventional and digital) and the crown fabrication method, considering the marginal and internal fit of the crowns fabricated by any combination of those methods. 5 CHAPTER II LITERATURE REVIEW Background of full-coverage restorations Full-coverage dental restorations are integral part of fixed prosthodontics. The reasons that compel patients to seek any type of dental restorations including inlays, onlay, veneers and crowns, could be divided into the following: 1) Dental disease including caries or periodontal causes, 2) Trauma such as accidents and 3) Esthetics to improve the appearance of their smile. The number of crowns delivered in a prosthodontic practice is high because their use can be incorporated into any treatment plan with any combination of teeth, dental implants and removable prostheses. Throughout the years, crowns have been the restoration most studied with regard to longevity, causes of failures, materials, and techniques; thus, there is some evidence to support their successful clinical use. Materials used for full-coverage dental restorations The restorations that prosthodontists use in clinical practice include full coverage crowns, veneers, onlays, inlays and fixed partial dentures. Along with the diversity of the restoration type, comes the diversity of materials used to produce each one of those types. Many materials have been used throughout the history of fixed prosthodontics, and each one presents its own indications, advantages and disadvantages. Currently, based on the most commonly used materials, restorations could be categorized as cast veneer (full gold), metal-ceramic and all-ceramic. 6 All-ceramic With the increased demand for more esthetic appearance, all-ceramic restorations have become very popular over the last decades. Such restorative all-ceramic systems must fulfill biomechanical requirements and should provide longevity similar to metal– ceramic restorations while providing enhanced esthetics (66). Dental ceramics can be classified according to their fusion temperature, application, fabrication technique and crystalline phase (41). According to Kelly, there are two concepts behind the science of ceramics used in dentistry (68). The first concept includes three main groups: predominantly glassy materials, particle-filled glasses and polycrystalline ceramics and the second concept includes any composition of two or more of those groups. The ceramics that best mimic the optical properties of enamel and dentin belong to the first group and the filler particles that improve the mechanical properties and control optical results belong to the second group. A special subgroup in the second category is glass-ceramic, such as the crystalline lithium disilicate filler. The third group has no glassy components and is much tougher and stronger than glassy materials; these are used as substructure materials (cores) upon which glassy ceramics are veneered, including glass-infiltrated alumina ceramic, polycrystalline alumina ceramic, lithium disilicate based glass–ceramic (75) as well as the recently introduced yttria-stabilized zirconia polycrystals (Y-TZP) (76). After the core has been fabricated, veneering porcelain is placed in order to give esthetic result most closely matched to natural tooth appearance. 7 Culp and McLaren presented three disadvantages of veneered all-ceramic restorations (75). The first disadvantage is the high value and increased opacity of the substructure material, which prevent it from being used for the whole thickness of the prosthesis especially in aesthetic areas. The second disadvantage is that although the high-strength material has excellent mechanical properties, the layering ceramic, to which is veneered, exhibits a much lower flexural strength and fracture toughness; for example, the zirconia core (900-1000 MPa flexural strength) is less than half of the cross-sectional width of a restoration. Veneering material, which has only 80-110 MPa, covers the rest and inevitably it tends to chip or fracture (77). The same concept applies to lithium disilicate, which has flexural strength of 440 MPa, and the nano-fluorapatite glassceramic (IPS e.max Ceram) that is layered upon it has flexural strength of 80-100 MPa. The third disadvantage is the combination of two incompatible materials in a synergistic way, whether that combination is metal with metal-ceramic or zirconia with zirconia layering ceramic, where a strong bond is difficult to create since it depends on several variables (cleanliness of bond surface, furnace calibration, cooling schedule, operator’s experience) (75). The interaction of core and veneer material can be complex. Fahmy evaluated the influence of three different veneering materials (Vitadur-N, Vitadur-α, and VM7) on the marginal fit and fracture resistance of an alumina core (In-Ceram) (78). The gaps were measured before and after veneering. The results showed that Vitadur-N veneered cores had statistically significant larger marginal gaps and decreased fracture resistance, whereas no significant difference was established between the other two materials tested. The fracture patterns were different with Vitadur-N veneered cores showing 8 delaminations, while the other groups showed complete failure involving both core and veneer. After the specimens were fractured, selected fragments were examined using SEM. For the Vitadur-N veneered crowns, the core/veneer interface appeared clear and evident with multiple spaces showing incomplete adherence. For the Vitadur-α group, the boundary appeared to have no gaps suggesting a good bond between the veneer and the core. For the VM7 veneered crowns, the boundary appeared evident; however, an apparent interlocking between the core and the veneer was present. The study concluded that veneering materials might alter the properties of alumina cores with regard to fit, strength and failure pattern. Because of these disadvantages, current research has focused on transitioning towards increasing the volume percentage of crystalline material with less or even no glassy components. Full polycrystalline ceramics have become practical for fixed prostheses as a result of the use of highly controlled starting powders and the application of computers to ceramic processing. IPS e.max all-ceramic Ivoclar Vivadent (AG, Schaan, Liechtenstein) first developed IPS Empress which is a leucite-reinforced glass ceramic. It is used with the hot-pressing technique that involves the use of a precolored glass-leucite ingot that is heated and pressed into a phosphate-bonded investment. Although, this technique produces a restoration with good marginal adaptation, the strength of the material itself has limited its use to single unit full-coverage restorations in the anterior segment (79-81); in the posterior segment it can only be used as onlays or partial veneer crowns with 97.1% survival rate in 3 years (82). 9 The same company introduced IPS Empress 2, in 1998. It was a lithium-silicate based glass ceramic (SiO2-Li2O) using the hot pressing procedure with in vitro mean fitting accuracy of posterior crowns amounted to less than 50 µm (83). The survival rate of this new all-ceramic material was found to be 100% for posterior single crowns and 70% for 3-unit FDPs, in the anterior and premolar area, in a 5-year prospective clinical study (84). IPS e.max lithium disilicate was introduced in 2005, as an improved hot-pressed ceramic material, in order to expand the range of indications of the previously used IPS Empress 2. This lithium disilicate is a glass ceramic that is composed of quartz, lithium dioxide, phosphor oxide, alumina, potassium oxide and other components. The properties that the material possesses include high flexural strength (360 MPa to 440 MPa), high fracture toughness (2-3 MPa) and high thermal shock resistance due to the low thermal expansion (85). Lithium disilicate can be processed using either lost-wax hot pressing techniques (IPS e.max Press) or CAD/CAM milling procedures (IPS e.max CAD). The pressable lithium disilicate (IPS e.max Press) consists of approximately 70% needle-like lithium disilicate crystals embedded in a glassy matrix. The desired color is controlled by the use of homogeneously distributed polyvalent ions that are dissolved in that matrix (85). On the other hand, the machineable lithium disilicate (IPS e.max CAD) consists of 40% platelet-shaped lithium metasilicate crystals embedded in a glassy phase which is produced after an “intermediate” crystallization process (blue, translucent state). The color is again controlled by the use of coloring ions with the difference being the oxidation state (intermediate phase); thus a blue color is produced. The final crystallized 10 state and desired tooth color is achieved during the post milling firing process in which lithium metasilicate transforms into lithium disilicate (85). There are several different IPS E.max ingots with different opacity and translucency levels; high opacity (HO), medium opacity (MO), low translucency (LT), and high translucency (HT). IPS e.max Ceram is the nano-fluorapatite layering ceramic material that is provided by the manufacture in order to create more esthetically acceptable restorations when HO or MO ingots are chosen (85). The IPS e.max CAD blocks are partially crystalized with moderate strength and easy to machine. The two levels of translucency depend on the crystallization pretreatment. The HT blocks contain few and large crystals of lithium metasilicate in the pre-crystallized state, whereas the LT blocks contain a high density of small crystals (65). As mentioned above, a disadvantage of all ceramic materials is the inevitable combination of dissimilar materials (core and veneering porcelain) in order to achieve optimum esthetics. With regard to that, lithium disilicate provides the option of a monolithic/fully anatomical restoration fabricated exclusively from this material. This produces a final restoration with high strength and good esthetics; however, surface colorants or even a partial layering technique may still be incorporated in particularly aesthetically demanding cases. Nevertheless, disadvantages, previously discussed, such as chipping of the veneering porcelain, are eliminated (86). Additionally, Guess et al. showed that IPS e.max CAD as a monolithic/fully anatomical configuration resulted in fatigue-resistant crowns, whereas the IPS e.max ZirCAD veneered IPS e.max Ceram crowns revealed a high susceptibility to mouth-motion cyclic loading with early veneer failures (chipping) (87). Another, very recent, use of the IPS e.max CAD, that needs to 11 be mentioned at this point, is as the actual veneer material for zirconia frameworks. Schmitter et al. assessed the ultimate load to failure of zirconia-based crowns veneered with IPS e.max CAD (CAD-on technology), compared to the ones veneered using conventional manual layering techniques (88). For the CAD-on technology, after the zirconia framework and the veneer structure were milled separately, fusion ceramic (IPS e.max CAD Crystal/Connect) was applied to the interior veneer surface and dispersed by means of vibration. Then, the assembly of framework and veneer was joined by pressure and vibration until the fusion ceramic mass was processed (i.e. turned flowable). At last, fusion, crystallization and glaze firing took place. For the layering technique, the framework was manually veneered using conventional veneering porcelain, which is recommended for zirconia cores. The results showed that the CAD/CAM veneers were non-sensitive to artificial ageing; with ultimate loads of failure for this particular material being above 1600N, concluding that this type of material may offer a way to reduce failures resulting from chipping of the veneering porcelain. Etman evaluated the relationship between crack propagation and ceramic microstructure following cyclic fatigue loading (89). The ceramics tested were AllCeram, IPS e.max Press and Sensation. There was a statistically significant difference among the three materials with the lithium disilicate material showing the highest resistance to crack formation and propagation. This has been attributed to the crystalline phases which have been reported to act as “crack stoppers” to prevent crack propagation. Table 1, included in Wiedhahn’s paper, summarizes and compares some of the properties of contemporary all-ceramics (90): 12 Glass- Lithium-disilicate Zirconia Ceramic VITA IPS IPS IPS IPS e.max ZirCAD/ MKII/IPS e.max e.max e.max InCeram YZ CAD LT CAD MO Press 120-160 360+/-60 360+/-60 440+/-40 800-1000 1.2-1.4 2-2.5 2-2.5 2.5-3 5.5-5.9 50-75 70-80 80-90 70-80 94 no no yes optional yes Empress CAD Flexural strength (3-point MPa) Fracture toughness (MPa m1/2) Translucency (CR Value %) Veneering Cementation Use adhesive conventional Chairside/ Chairside/ Lab Lab Lab conventional Lab Table 1. Properties of contemporary all-ceramic materials. Lab 13 Longevity of all-ceramic restorations Several studies reviewed the long-term performance of all-ceramic crowns by comparing the different systems with each other or with the metal-ceramic prostheses. However, different criteria, different methodologies and different statistical analyses were used in the existing studies and do not allow for direct comparisons between them. Pjetursson et al. published a systematic review of all-ceramic and metal-ceramic crowns. After the exclusion criteria were applied, a final number of 34 (28 all-ceramics and 6 metal-ceramics) studies were evaluated (91). The authors were surprised to find that there were only 6 studies evaluating long-term survival of metal-ceramic restorations available and moreover, that four of them had been published very recently. In metaanalysis, the 5-year survival of all-ceramic crowns was estimated to be 93.3% and 95.6% for metal–ceramic crowns. All-ceramic crowns were also analyzed according to the material utilized; Procera crowns showed the highest 5-year survival rate of 96.4%, followed by reinforced glass-ceramic (Empress) and InCeram crowns with survival rates of 95.4% and 94.5%, respectively. A significantly lower survival rate of 87.5% was calculated for Empress crowns after 5 years. All-ceramic crowns were also grouped and analyzed according to the position in the mouth. Lower survival rates were found when crowns were seated on posterior teeth; for Empress crowns (84.4%) and InCeram crowns (90.4%), this difference reached statistical significance. In the same analysis, the most frequent complication for all-ceramic crowns was core fracture, leading to 85% of the losses. With the exception of ceramic chipping, the incidences of technical complications like loss of retention and ceramic fracture causing loss of the crown were lower for metal-ceramic crowns than for all-ceramic crowns. On 14 the other hand, biological complications like caries, periodontitis and abutment tooth fracture were more frequent in the metal-ceramic group, which is expected based on the considerably longer exposure time of the metal-ceramic crowns (mean exposure time for metal-ceramic crowns (9.2 years) was almost twice as long as that of the all-ceramic crowns (4.9 years). Guess et al. in a 5-year prospective clinical splitmouth investigation evaluated the survival rate and long-term behavior of IPS e.max Press (IP) and ProCAD (PC) allceramic partial coverage restorations (PCRs) on molars (92). Forty PC and forty IP PCRs were inserted in 25 patients. The modified United States Public Health Service (USPHSAlpha, Bravo, Charlie) criteria were used at baseline and after 13, 25, and 36 months post-insertion. The Kaplan–Meier survival probability of the PC restorations was 97% (1 mandibular PC-PCR demonstrated an absolute failure by a clinically unacceptable marginal ceramic fracture of the lingual-occlusal cusp area after a service time of 9 months). All IP restorations remained in situ indicating survival probability of 100%. Regarding the criterion marginal adaptation, a distinct deterioration was found over time, independently of the fabrication techniques and all-ceramic materials used; alpha ratings decreased from 95% to 83.3% for IPS e.max Press and from 92.5% to 65.2% for ProCAD restorations. Etman and Woolford, in another study, compared Procera AllCeram and IPS e.max Press to metal ceramic restorations, and the 3-year clinical evaluation indicated similar success rates for all 3 groups (93). The modified United States Public Health Services (USPHS) evaluation showed that the IPS e.max Press and metal-ceramic crowns experienced fewer clinical changes than Procera AllCeram. 15 Fasbinder et al. evaluated the 2-year clinical performance of chairside (Cerec 3, Sirona Dental Systems) lithium disilicate IPS e.max CAD all-ceramic crowns (94). They found no crown fracture or surface chipping, indicating that monolithic lithium disilicate CAD/CAM performed well after 2 years of service. Marginal and internal fit of dental restorations The accuracy of fit is the characteristic that is most closely related to the longevity of a restoration (1, 2). Ideally the cemented crown should precisely meet the finish line of the prepared tooth. In reality, clinical perfection is challenging to achieve and to verify. The importance of a well-fitting full-coverage restoration can be illustrated most clearly when considering the implications that occur with an ill-fitting restoration. Luting agent dissolution (4), microleakage (3, 6), caries (2, 3, 6), hypersensitivity and periodontal inflammation (5, 7) are the most common of such implications. Caries have been shown to be the most common reason (36.8%) for crown failure according to a 3-year clinical survey study by Schwartz et al. (2). Bader et al. found that plaque and calculus scores, gingival inflammation and bleeding were significantly higher on crowned teeth than uncrowned teeth (5). Reeves, also, reported that open margins and overhangs associated with subgingival crown margin location were areas where chronic inflammatory response and greater attachment loss could be expected (7). Although White et al. reported that marginal opening alone did not directly correlate with marginal microleakage (1), the accuracy of marginal fit is valued as one of the most important criteria for the clinical quality and success of prosthetic restorations. According to Holmes et al., “the fit of a casting can be defined best in terms of the 16 ‘misfit’ measured at various points between the casting surface and the tooth” (18). The definitions that they used to describe this “misfit” included internal gap, marginal gap, vertical marginal gap, horizontal marginal discrepancy, overextended margin, underextended margin, absolute marginal discrepancy and seating discrepancy. Christensen found, using a linear regression prediction formula, that an acceptable gingival margin range is 34-119µm (95). McLean and von Faunhofer, also, suggested that restorations with marginal gap less 120µm are more likely to be successful (8). Several studies have investigated the internal fit with regard to crown adaptation (8-17). Specifically, Fusayama et al. showed that internal relief with either manicure liquid or tinfoil (40µm thickness) improved the seating of complete cast crowns regardless of whether a complete or partial relief was used (17). Eames et al. discovered that a 25µm thickness of a die spacer not only improved the casting seating but also increased retention by 25% (10). Grajower and Lewinstein said that the thickness of the spacer should allow for the cement film thickness, roughness of the tooth and casting surfaces, dimensional inaccuracies of the die and distortions of the wax pattern (11). Wilson showed that there was a significant correlation between increased spacing and decreased seating time and decreased seating discrepancy (12). He also concluded that spacing of less than 40µm prevented the crown from seating well before the set of the cement, which resulted in marginal discrepancy. Olivera and Saito evaluated the effect of die spacer on the fit and retention of complete cast crowns by using three different cements (13). They used four layers of die spacer using three different techniques: covering the occlusal and 1/3 of the axial surfaces, covering the occlusal and 2/3 of the axial surfaces and covering the entire 17 preparation except the apical 0.5 mm. The crowns were assigned to one of three luting agents: resin modified glass-ionomer cement, resin cement and zinc phosphate. The results showed that better marginal fit was obtained when the die spacer covered all but the area 0.5mm short of the margin of the preparation; however after cementation the resin modified glass-ionomer cement had the best fit with the same application of die spacer. Another aspect of the importance of the internal fit of the crowns is its effect on fracture resistance of all-ceramic restorations. More specifically, Tuntiprawon and Wilson evaluated the effect of increasing cement thickness (using platinum foil and die spacer) on the fracture strength of all-ceramic crowns (14). Each crown was cemented onto a metal die with zinc phosphate cement and loaded until fracture. They found that a decrease in strength was observed with increase of cement thickness and the strength decrease was possibly attributed to the greater deformation of the porcelain into the cement as well as the decreased thickness of the crown itself. Bottino et al. studied the cervical adaptation of metal crowns with regard to the influence of cervical finish line, internal relief and cement type (15). They compared three full crown preparations (chamfer, 135-degree shoulder and rounded shoulder), internal relief of 30µm 0.5mm above the finish line vs. none, three types of cement (zinc phosphate, glass-ionomer and resin cement). Significant influence of all three variables tested was observed on the cervical adaptation. The least marginal discrepancy was achieved with chamfer finish line, internal relief and use of glass-ionomer cement. Their results showed that in order to optimize the cervical adaptation and enhance the complete seating of the crown, creating a correct finish line, the use of a minimum thickness die 18 spacer 1 mm above the cervical line, using the cement with favorable flow and adequate load application during cementation are all essential for a successful restoration. A different factor that has been investigated by Ayad is the effect of tooth preparation burs on the marginal adaptation (16). In that study diamond, tungsten carbide finishing and crosscut carbide burs of similar shape were used in combination with different luting cements: zinc phosphate cement (Fleck’s), glass ionomer cement (KetacCem), and adhesive resin cement (Panavia 21). Marginal fit was measured with a light microscope in a plane parallel to the tooth surface before and after cementation. Results revealed a statistically significant difference for burs used to finish tooth preparations, whereas luting cement measurements were not significantly different. Ayad showed that tooth preparations refined with finishing burs may favor the placement of restorations with the smallest marginal discrepancies, regardless of the type of cement used. Methods to measure the marginal gap In the literature several techniques have been suggested for the measurement of the marginal fit alone or in combination with the internal fit of crowns. All of these present advantages and disadvantages, and a small description of the most commonly used ones will follow. To begin, the dental explorer is the most common tool used to detect marginal adaptation, as it is often the only clinical instrument available. Hayashi et al. studied the influence of the explorer tip diameter and the visual condition in evaluating vertical steps and horizontal gaps (20, 21). A significant correlation was found between explorer tip diameter and Alpha/Bravo boundary for horizontal gaps, but not for vertical steps. There 19 was no significant difference among the visual conditions tested. Apart from the explorer tip, the amount of clinical experience seems to exert the greatest influence on the identification of those gaps. The latter is further supported by another study, which showed that operators with clinical experience had a threshold that detected crowns with a smaller gap while maintaining a higher degree of consistency in their personal judgment than did the operators without any clinical experience (22). This finding, according to the authors, suggests that the use of the explorer is characterized by subjectivity, introducing the bias of the investigator. Apart from that limitation, this technique becomes even less accurate with subgingivally placed margins where, according to Christensen, in an ideal environment, the acceptable mean opening of subgingivally marginated cast restorations was shown to be almost 3.5 times the acceptable mean opening of supragingival margins (95). The radiograph could also be considered a tool that would provide information with regard to the marginal fit of the crown; again most importantly during the clinical practice. Assif et al. compared the tactile method (the use of explorer) to the use of radiographs and to a technique using impression material in order to examine the marginal fit (23). The results supported that neither the explorer nor the radiographs were superior in detecting discrepancies, with the impression technique presenting the most accurate data of the three. A technique used in vitro is the classic destructive method of sectioning the specimens and then studying them under an optical or scanning electron microscope (24, 25). The advantage of this technique is the accuracy and the precision in repeatability of the measurements; however the obvious limitations of this method are the destruction of 20 the specimens which creates the need for duplicates, the limited area that is evaluated since the sections have a minimum thickness and the additional steps that are required (embedding in resin and sectioning). Romeo et al. used a stereomicroscope under 50x magnification to measure the marginal adaptation of CAD/CAM restorations (26). Photographs were taken under the microscope and the measurements were performed with PC software. Despite the nondestructive nature of the method, which is very favorable, it is very technique-sensitive; a slight deviation of the photographic angle will distort the measurement. Specifically, because the microscope is set perpendicular to the margin of the restoration, it makes it impossible to evaluate the marginal gap of an overhanging restoration (vertical overextension); thus, a 0µm value would not necessarily mean that the restoration has a perfect fit. Tan et al. used a similar setting (and therefore similar technique limitation) in order to compare the marginal adaptation of CAD/CAM, wax/CAM and wax/cast restorations (27). They took a 1:1 photograph of each of four sides of the die using a digital camera mounted on a tripod. All photographs were taken sequentially with no change in the horizontal inclination of the camera. Calibrated digital measurement software was used to measure the marginal openings. Gonzalo et al. measured the external marginal fit of zirconia vs. metal-ceramic posterior FPDs with two different measurement methods: 1) An image analysis (IA) system (software and microscope) and 2) A scanning electron microscope (28). The twoway repeated measures ANOVA showed significant interaction between measurement method and material, with the measurements obtained with IA values being lower than 21 those obtained with SEM except from metal-ceramic FPDs. The authors concluded that the measurement method was not independent of the material, and therefore a standardized method to analyze marginal fit of full coverage restorations should be established. Mitchell et al. compared, in vitro, the marginal fit of 4 types of complete crowns on human premolar teeth with the use of a nondestructive technique called profilometry (29). This method determined whether the fit was influenced by type of the crown or surface morphology of the tooth (grooved or ungrooved surfaces). It was found to be an accurate method, but considering that this technique does not identify cases of vertical overextension, the results once again are subjected to false interpretation. A technique that has been suggested by Pelekanos et al. was the computerized xray microtomography, where multiple projections of an object were taken as the source rotated around it (30). The projections were transferred to a computer and with special software; small slices of the object’s internal structure could be added to the object’s 3-D image. Advantages of this technique included the ability to produce images of the internal structure of the specimen, in section form, while allowing for 3-D reconstruction, and the possibility of obtaining very proximate sections. On the other hand, the disadvantages that this method presented included the low capacity of discrimination of CT microtomography in comparison with the optical or electron microscope, the possible artifacts from refraction of the images from radiation, the compulsory radiopacity of the material tested and the difficulty to define the materials that have different coefficients of absorption. 22 The most commonly found nondestructive technique in literature is the replica technique (RT) (31-37). Replicas of the intermediate space between the inner surface of the crown and tooth surface are made by the method described by Molin and Karlsson (31). The important characteristic of this technique is that it can be used in vivo as well as in vitro since it does not involve the destruction of the specimens. Boening et al., using an RT based on the previously mentioned study, measured in vivo the marginal fit of Procera AllCeram crowns using a light body silicone to fill space between crown and tooth and a heavy body silicone to stabilize the light body film (32). After removal from the artificial crowns, the replicas were segmented, and measurements of the film thickness were performed with a light microscope. The main limitation of this method is the distortion or even the damage of the material during manipulation. A very interesting study was conducted to evaluate the validity of the replica technique after its wide use (96). The purpose of that study was to compare the film thickness of the cement resulting from the cementation of Procera copings with the film thickness obtained from the light body silicone using the RT. The RT, using a light body silicone impression material instead of cement, was developed based on the assumption the two materials behave in a similar way in terms of film thickness, despite the different nature and different physical and mechanical properties that they possess (36). In that study, replicas were fabricated by the technique described by Molin and Karlsson (31); the same copings were cemented with glass-ionomer cement and then were sectioned. All cuts produced by both techniques were measured under an optical microscope. The results showed that the mean values measured with the RT were similar to the ones measured using glass-ionomer cement, and no significant difference was established for 23 either the different groups (premolars and incisors) or the different areas (occlusal, axial, and cervical) tested. Therefore, the authors concluded that the RT is accurate and reliable for reproducing cement film thickness, and the use of this technique for the measurement of crown gap spaces can be recommended. More recently the measurement using the RT has changed. Luthardt et al. developed an indirect technique in order to achieve 3-D analysis of the internal space between the crown and the metal master die (38). The method used gypsum duplicate dies along with silicon films (replicas) that represent the internal surface of the crowns, which were, then, digitized using the same measuring position. As technology evolved further, a new method of 3D fit assessment for dental restorations has been proposed. Holst et al. developed a new triple-scan protocol; using a non-contact scanner, three scans were performed: 1) Coping solo, 2) Master cast solo and 3) Coping placed on master cast in a clinically correct final position (97). After the objects (coping and master cast) were digitized, surface tessellation/triangulation language (STL) surfaces were generated from point clouds with the scanner software (ATOS system, GOM mbH, Braunschweig, Germany). First, the master cast STL and the coping-master cast STL were registered by manual alignment followed by best-fit registration. Then the same was done to match the coping and coping-master cast. The coping-master cast data were deleted and the aligned coping-master cast data were used for fit assessment. To measure the cement gap, the outer surface of the coping was deleted, followed by reversion of the surface normal of the intaglio of the coping and calculation of deviations from the master cast. The investigators obtained the mean value of the cement space for 50 copings on their respective abutments and they repeated the 24 measurement three times. Their results showed no significant differences among these measurements (p=.170), Pearson correlation coefficient revealed almost perfect associations (r=1) and intraclass correlation coefficient also revealed almost perfect coefficient for repeatability (r=0.981, p<.001). The same main investigator used this protocol in order to assess the precision of fit of CAD/CAM dental implant superstructures (39). The statistical analysis, again similar to the previous study, resulted in an intraclass correlation of 0.991 (95%-CI between 0.978 und 0.998) and therefore a statistically significant repeatability of measurements (p<0.001, F = 112.95). Both of those studies proved that the triple-scan protocol was a highly reliable approach in order to evaluate the 3D fit of both conventional and implant retained restorations. The disadvantages of 2D measurements were eliminated, while the advantages of the non-destructive approach of this method made it a superior alternative for future dental research. The one inherent problem of all non-contact optical digitizers is the accuracy and capability to actually capture the different surfaces/materials. Usually for highly reflective or translucent materials (such as all-ceramic crowns) the surfaces need to be coated with a contrast agent which may introduce error. However, for comparative studies the absolute values might not be of great importance. Where high accuracy measurements are needed, a high precision coordinate measurement machine (CMM) integrated with 3D laser scanning system should be used (98). That non-contact measurement method consists of a laser sensor that is mounted to a 3-6-axis computer controlled positioning system (i.e. CMM). The Surveyor ZS-Series scanner with scan accuracy (accuracy describes how closely the measurements of the 25 laser conform to the true dimensions of the object being measured) of +/- 0.00898mm (Laser Design Inc, GKS, Minneapolis, MN, USA) is such a system that was used in this study. The principal behind the laser scanning is called Laser Triangulation, (Figure 1). Laser triangulation is an active stereoscopic technique that computes the distance of an object with a directional light source and a video camera. A laser beam is deflected from a mirror onto a scanning object. The object scatters the light, which is then collected by a video camera located at a known triangulation distance from the laser. Using trigonometry, the 3D spatial (XYZ) coordinates of a surface point are calculated. The charge-coupled device (CCD) camera’s 2D array captures the surface profile’s image and digitizes all data points along the laser (98). Figure 1. Laser Triangulation. Available from: http://www.laserdesign.com/resources_and_downloads/faq/ 26 The shape of the single “2D” profile is recorded by the digital CCD and subsequently, based on the calibration and look up tables of the lasers; a Z position is determined and stored for each pixel value by the software. This location along with the machine axes positions are used to compute the X,Y,Z coordinates of the points along that profile. Hundreds of thousands of similar profiles are thus collected as the probe sweeps over the object and the software stores this information into a database for later retrieval. Each profile comes into the database as a single polyline entity with points distributed along the length of the line. These polylines are displayed graphically on the computer screen as they are gathered (98). Once the data (points) are acquired Digital Shape Scanning and Processing (DSSP) is applied using sophisticated processing software (i.e. Geomagic Qualify 12, Geomagic, Research Triangle Park, NC, USA) (99). DSSP is based on two main technology developments: scanners and other hardware to capture point data and software to process the data into useful forms. Each point measurement is subject to a wide variety of measurement errors. While sources of error (contamination on the part, vibration, operator error, etc.) are on the same order of magnitude as traditional measurement methods, the sheer volume of point cloud data requires sophisticated algorithms to catch problems, such as minute surface defects, and properly register the entire shape. Point cloud data may be converted to an accurate polygonal mesh or a surface representation (typically NURBS, used by most CAD systems) (99). 27 Subsequently, the 3D digital object can be processed and analyzed accordingly; automated reports, including 3D PDF, numeric inspection data, annotated user-defined views, notes and conclusions may be generated (100). A synopsis of a flow diagram using this method includes: Test object > 3D scanning > Scan processing and merging > CAD object > Aligment > Comparison > Evaluation > Reporting (Statistics database, Print, Online) (99). In summary, the measuring techniques used for marginal and internal fit of crowns were categorized by Sorensen (1990) as follows: 1) Direct view, 2) Crosssectional, 3) Impression technique, and 4) Explorer and visual examination (19). However, with the advancement of the technology, new categories will be added. By digitizing the specimens (dies, silicon films or even the intaglio of the crown itself), the data sets acquired allow for several measurements and comparisons. Marginal adaptation of all-ceramics Despite the great variety of available restorative materials, the cast restorations continue to be considered the gold standard for vertical marginal adaptation (101, 102). Holden et al. compared a traditional metal-ceramic restoration (MCR) fabricated from feldspathic porcelain fused to metal, a leucite-glass ceramic pressed to metal restoration (PTM) and a restoration from all leucite-glass-pressed ceramic (PCR) with porcelain butt joints (69). All restorations were evaluated on their respective dies at 45x magnification using an Olympus SZX-12. The results showed that the MCR group had the highest values with regard to marginal openings, whereas the PTM group showed the best marginal adaptation. The restorations with pressed ceramic margins may be less 28 technique sensitive for the laboratory to fabricate when compared to the traditional metalceramic and that may be the reason for the difference on the adaptation. Yeo et al. evaluated, in vitro, the marginal discrepancies of 3 different all-ceramic crown systems (Celay In-Ceram, conventional In-Ceram, and IPS Empress 2 layering technique) in comparison to a control group of metal ceramic restorations using an optical microscope (103). The results indicated that the IPS Empress 2 system showed the smallest and most homogeneous gap dimension, whereas the conventional In-Ceram system presented the largest and more variable gap dimension compared with the metal ceramic (control) restoration. Baig et al. compared the marginal fit with respect to gap and overhang, at 6 designated margin locations of zirconia (Cercon Y-TZP) ceramic crowns to lithium disilicate pressable (IPS Empress II) and complete metal crowns (control group) using a computerized digital image analysis system (72). It was found that the mean marginal gap for those three groups was 66.4µm, 36.6µm and 37.1µm, respectively. The 2-way ANOVA revealed significant differences in marginal gap between the machinable ceramic (Cercon) and pressed ceramic system (IPS Empress II) and complete metal crowns (control). The significantly higher marginal gap of the Cercon crowns compared to IPS Empress II crowns was attributed to the type of manufacturing of these two ceramic systems. Specifically, the distortion of the ceramic coping was thought to be less in IPS Empress II compared with Cercon, as the pressed technique involves a less complicated process, using a more manual than computer program computation, and is process dependent. 29 Stappert et al. investigated the marginal fit of IPS e.max Press material by fabricating partial coverage restorations on teeth with different preparation designs (104). The restorations were adhesively luted and exposed to a mastication simulator. The discrepancies of the marginal fit were examined on epoxy replicas before and after luting as well as after masticatory simulation at 200x magnification. The authors found that before cementation the mean (geometrical) marginal gap width consistently decreased as the dimensions of the restorations increased (mean range 84.3-50.3µm), the cementation increased the marginal gap of most of the groups except one (mean range 104.7-93.8µm) and the masticatory loading increased the gap of all groups but significantly only to two preparation designs (mean range 117.5-106.1µm). The conclusions of this study point out that the IPS e.max Press (VP 1989) can be used to fabricate inlays and partial crowns which meet the requirements in terms of a clinically acceptable marginal gap, irrespective of the preparation design used, given that all mean marginal gaps were below 120µm. However, the preparation design and dimensions of the restorations appeared to affect the initial marginal fit and escape of luting material during the cementation process. In another study, Stappert et al. evaluated the marginal accuracy of partial coverage restorations under mouth-motion fatigue and thermal cycling (73). The materials tested were: gold (GO), hybrid composite (Targis) (TA), IPS e.max Press (EX), IPS-Empress (EM) and ProCAD/Cerec( PC). After the cementation the mean marginal fit was: GO-47 [43–51]µm, TA-42 [38–45]µm, EX-60 [52–67]µm, EM-52 [45–60]µm and PC-75 [59–94]µm, indicating that IPS e.max Press had similar marginal fit to gold and IPS-Empress, whereas the ProCAD group demonstrated poorer marginal fit. Similar results were found after the aging test (GO-42 [38–45]µm, TA-42 [38–47]µm, EX-56 30 [49–65]µm, EM-54 [46–64]µm and PC-71 [59–84]µm) without compromising the marginal fit of the restorations under the given testing conditions. Dental impressions The dental impression is of great importance in dentistry in general, and in fixed prosthodontics in particular. The materials and techniques have gone through major evolution throughout the years. At the beginning, rigid materials including zinc oxide eugenol paste, wax, modeling compound and impression plaster were used. Because of the obvious rigidity, distortion and breakage that occurred, their use was significantly reduced during the later years. In the 20th century, elastomeric materials were introduced. They are classified as aqueous and non-aqueous elastomers. The first category consists of the reversible hydrocolloid (agar) and the irreversible hydrocolloid (alginate). Agar is dimensionally unstable thus casts must be poured immediately. Alginate is the most commonly used material for diagnostic impressions, mainly because it is inexpensive. However, it is also dimensionally unstable and it must be poured within 10 minutes (40). The second category, non-aqueous, consists of polysulfides (1950), condensation silicones (1955), polyethers (1965) and addition silicones (1975). Polysulfides were also called “rubber base”; they reproduced details with excellent results, were not rigid and captured subgingival margins but they were dimensionally unstable, did not have good elastic recovery and had long setting time. For the condensation silicones, the release of ethyl alcohol during polymerization that causes shrinkage was the main disadvantage. However, it was shown that this shrinkage was greater in the low viscosity than in the putty-like viscosity. Polyethers are hydrophilic (contact angle 49 degrees); thus they have 31 superior detail reproduction in the presence of moisture. They are, also dimensionally stable and they provide an excellent reproduction of detail. However, strict disinfection guidelines should be respected in order to prevent expansion. Also, their rigidity makes them more difficult to remove than addition silicones and more likely to fracture delicate gypsum dies. Addition silicones, Polyvinyl siloxanes (PVS), have become the most widely used impression material in dentistry (42). They have the best detail reproduction and elastic recovery of all available materials, and their dimensional stability allows multiple pours; thus, PVS materials are the materials of choice in fixed prosthodontics (42, 43). They are moderately rigid, have good tear strength, relatively short setting time and can be used with most disinfection protocols. Their disadvantages include susceptibility to contamination as a result of sulfur and sulfur compound and hydrophobic behavior (contact angle 98 degrees) caused by hydrophobic aliphatic hydrocarbon groups around the siloxane bond. Today, in order to overcome this, nonionic surfactants (nonylphenoxypolyethannol homologues) have been incorporated and the new PVS materials, have improved wettability (contact angle 53 degrees); however they are still clinically acceptable only in dry conditions (41). According to the ANSI/ADA Specification No. 19 (ISO 4823) regarding detail reproduction, all elastomeric materials, except from very high-viscosity products, should reproduce a V-shape groove and a 0.02mm wide line (41). Walker et al. evaluated the detail reproduction of polyether and PVS material by observing the continuous replication of at least two out of three horizontal lines (105). The impressions were made under dry and moist conditions. They found that under dry conditions all materials produced satisfactory detail reproduction 100% of the time; however under moisture only 32 29% of PVS materials produced satisfactory detail while 100% of polyether met the detail criteria. Dimensional stability over time allows the operator to pour the impression at any time. Thongthammachat et al. evaluated the influence on dimensional accuracy of dental casts made with different types of trays and impression materials when they were poured at different and multiple times (53). The researchers concluded that an impression made from polyether should be poured only once within one day after impression making because of the distortion of the material that occurs over time. Addition silicone impression materials had clearly better dimensional stability than polyether up to 720 hours which is in agreement with previous studies (46, 47). In the study, addition silicone also showed deviations increasing over time, but these were relatively small. The possibility of imbibiton should be considered- especially with polyether impression materials- due to the fact that polyether absorbs water from the gypsum and swells with each successive pour. Elastic recovery allows the material to return to its original dimensions when the impression is removed from the mouth. No contemporary material has 100% elastic recovery. PVS materials have the best elastic recovery at over 99% with a specific test undercut (106). In addition to the material, the impression technique is a factor that has been studied with relation to the influence on the success of the impression. Hung et al. reported that the accuracy of addition silicone was affected more by the type of materials than by the technique (48), while Johnson and Craig stated that accuracy could be better controlled with technique than by the material itself (45). The findings in the latter study 33 indicated that impressions made in stock trays, that is, the putty wash two-step technique with polyethylene spacer, were as accurate as those for impression techniques in custom trays. The use of custom tray versus a stock tray is a controversial issue. The rationale behind the need for custom tray is that it provides the essential uniform thickness of the impression material with different researchers suggesting different amount of space (1 to 5 mm). Although custom trays have been recommended to produce more accurate impressions, stock trays remain popular because they are readily available and easy to use. Valderhaug and Floystrand reported no significant difference in the linear dimensional stability of impressions made from custom and stock trays, even when the thickness of the material ranged from 2 to 9 mm (50). Rueda et al. examined the dimensional stability of several impression materials made from stock and custom trays by measuring the linear dimensions of the casts poured at 1 hour, 1 day and 1 week (51). There was a significant but very small difference in the linear dimensions of the casts produced with both impression materials using stock and custom tray (dimensions varied form 15 to 50 µm). The least amount of variation was noted when 2.0- 2.5 mm of spacer was used. Thongthammachat et al. also showed that if stock trays were properly oriented and the impression thickness was uniform, stock trays could give a better result than custom trays (53). The influence of dimensional change of the impression tray upon the accuracy of the impression material has been reported as well as the time when the adhesive should be applied ranging from 5 minutes to 48 hours, with the 48hr adhesive drying time exhibiting the highest mean adhesive tensile bond strength (52). 34 In Donovan and Chee’s review article three approaches to putty/wash impressions were presented (42). For the first approach, putty material was used to fabricate the custom tray by making an impression of the diagnostic cast. For the second, a preoperative putty impression was made intraorally, material was removed from the embrasures and a “wash” impression with low-viscosity material was made. The third approach involved “simultaneous” technique using a tray loaded with putty and a syringe with low viscosity used around the teeth. According to Chee and Donovan, the third approach is unacceptable because it is impossible to control the thickness of the impression material and what material records the margin detail of the preparation (44). Mishra and Chowdhary tested the following different impression techniques using polyvinyl siloxanes- group 1: putty wash two-step technique with polyethylene spacer, using a stock tray; group 2: putty wash one-step technique, using a stock tray; group 3: single-mix technique, utilizing medium viscosity in a custom tray; group 4: multiple-mix technique, utilizing a heavy and low-viscosity combination in custom trays (49). The oneway ANOVA revealed that all of the dimensions tested (anterior-posterior, lateral and vertical) were significantly different among the impression techniques. The findings of this study showed that group 4 produced the most accurate result in the anterior-posterior and vertical dimensions, followed by group 1 in the anterior-posterior dimension, and group 3 in the vertical dimension. Groups 1 and 3 were the most accurate in the lateral dimensions. Group 2 produced the least accurate results in all dimensions. The accuracy of group 4 was attributed to a controlled amount of bulk of impression material and adhesive systems and low polymerization contraction with the heavy-body material, compared with the light-body products due to a greater concentration of inert fillers. The 35 increased lateral dimension, as compared to the master model that was found for group 4 was possibly due to the contraction of the impression material toward the tray. According to Eames and Sieweke (107) and Lewinstein (108), the impression material contraction toward the tray wall may produce stone dies wider horizontally (interabutment) and shorter vertically. In summary, several materials and techniques have been used for impressing soft and hard oral tissues. All present advantages and disadvantages; knowledge and experience lead to proper selection and successful results. Dental plaster and stone Gypsum products are widely used in dentistry. Dental plaster, stone, highstrength/high expansion stone, and casting investment constitute this group of closely related products. All forms are derived from natural gypsum deposits with the main difference being the manner of driving off part of the water from the calcium sulfate dihydrate. The most important and well-organized theory for the mechanism of the setting is the crystalline theory; the setting reaction of the water with calcium sulfate hemidrate to form calcium sulfate dihydrate caused by the difference in solubility between those two components. Regardless of the type of gypsum product employed, an expansion of the mass can be detected during the change from hemidrate to dihydrate. Depending on the composition of the gypsum product, the observed linear expansion may be as low as 0.06% to as high as 0.5%. On the other hand, if equivalent volumes of the materials used for this reaction are compared, the volume of the dihydrate formed will be less than the equivalent volumes of the hemidrate and water; thus a volumetric reaction 36 should occur during setting. However, a setting expansion is observed and this phenomenon can be rationalized on the basis of the crystallization mechanism. This expansion is affected by multiple factors such as water to powder ratio, spatulation (speed or time), temperature (water and environment), humidity, presence of colloidal systems (agar and alginate) and pH (such as saliva) (41). According to the ANSI/ADA Specification No.25 (ISO 6873) there are 5 types of gypsum (41). Type I is the impression plaster, but it is rarely used today because less rigid impression materials are available. Type II is model plaster, which is now used in prosthodontics principally to fill flasks in denture construction, where setting expansion, is not critical. Type III, dental stone, has a minimum one-hour compressive strength of 20.7MPa, but it does not exceed 34.5Mpa. Type III gypsum can be used for diagnostic casts. Type IV is high-strength/low expansion dental stone and it is used for die stone, which needs strength, hardness, abrasion resistance, and minimum setting expansion. Type V is a high-strength/high-expansion dental stone. Setting expansion is increased from a maximum of 0.15% to 0.30. Several physical properties should be taken into consideration when a die material is used, such as linear dimensional change, detail reproduction, surface hardness, abrasion resistance and transverse strength. Kenyon et al. compared the linear dimensional accuracy of 7 die materials including Vel-Mix (Type IV), Hard Rock (Type V), Resin Rock (Type IV- resin impregnated), Die Epoxy fast set (Epoxy resin), ModelTech (Polyurethane resin), Integrity (Bis-Acryl Composite) and Copper-plated (109). Resin Rock and copper-plated dies most closely approximated the dimensions of the master die and were not significantly different from each other in any of the three 37 measurements but they were significantly different from the other 5 for at least one measurement. Type IV and V dental stones exhibited setting expansion within the range appropriate for gypsum. Both impression material and die material are essential for the production a consistent and reliable cast or die. The fit and the ultimate clinical success of an indirect restoration depend on the accuracy of the die reproduction, which is subject to possible deformation during the prosthesis fabrication process. Ragain et al. compared the detail reproduction, contact angle and surface hardness between various combinations of contemporary elastomeric (PVS, condensation silicon, polyether, polysulfide and reversible hydrocolloid) and die materials (Type IV, V and Resin-reinforced Type IVResin Rock) (110). According to the findings of this study, when using PVS or polyether, a Resin Rock stone may produce dies that have less scratch resistance on the surface than the Type IV die stone. Overall, no combination was identified to be superior for all the parameters tested. Casts and die materials must reproduce an impression accurately and remain dimensionally stable under normal storage and usage conditions (41). The particular impression material used and the purpose that is made for, determine the selection among the gypsum products. Computer-Aided Design/Computer-Aided Manufacturing (CAD/CAM) systems The clinical success of the all-ceramic restorations depends not only on the mechanical and physical properties of the materials used but also on the precision of the particular computer-aided-designed/computer-aided-manufacturing systems used to 38 fabricate those restorations. The application of CAD/CAM systems in dentistry had initiated as early as 1970s by Duret and colleagues, but it was not until the 1980s when Mormann and colleagues developed the CEREC system, that they gained popularity (74, 111). Today, a rapidly increasing number of milling systems have established themselves on the dental market (57, 74). Some of them are: • Cercon smart ceramics (DeguDent Frankfurt GmbH) • CEREC inLab (Sirona Dental Systems, LLC, Charlotte, NC, USA) • DCS Dental (DSC Dental AG, Allschwil, Germany) • E4D (D4D Technologies, Richardson, TX. USA) • Etkon (etkon AG, Gräfelfingen, Germany) • Hint Els DentaCAD systeme (Hint-Els GmbH) • KaVo Everest (KaVo Dental GmbH, Biberach, DEU) • LAVA (3M, St. Paul, MN, USA) • Nobel Biocare Procera (Nobel Biocare, Yorda Linda, CA, USA) • Wieland Zeno (Wieland Dental and Technik GmbH & Co. Pforzheim, DEU) • ZirkonZahn (GmbH, Gais, Italy) and many more (57, 74). Beuer et al. presented an overview of the parameters of available contemporary CAD/ CAM systems (56). To begin with, the components of all CAD/CAM systems include: 1. The scanner, which is the tool that measures tooth, structures and transforms them into digital data sets. Two categories of scanners are available the optical and the 39 mechanical ones. The optical scanners use the triangulation procedure, as described above, which consist the majority of the contemporary scanners in dentistry. The mechanical scanners use a ruby ball to measure the threedimensional object. The only scanner in this category is the Nobel Biocare Procera one (Nobel Biocare, Yorda Linda, CA, USA). 2. Design software that can design the crown and fixed partial denture framework or the fully anatomical restorations. 3. The processing device where the restoration will be milled. Those devices are categorized based on the number of milling axes. a. The 3-axis milling device has degrees of movement in the three spatial directions defined by the X -, Y -, and Z – values. The calculation investment is minimal, but a milling of subsections, axis divergences and convergences is not possible. All 3-axis devices used in the dental area can also turn the component by 180° in the course of processing the inside and the outside. The advantages of these milling devices are short milling times and simplified control by means of the three axes. Such devices are: inLab (Sirona), Lava (3M ESPE), Cercon brain (DeguDent). b. The 4-axis milling device has the three spatial axes as well as a rotatable tension bridge which helps to adjust bridge constructions with a large vertical height displacement into the usual mold dimensions and thus save material and milling time. Example: Zeno (Wieland-Imes). c. The 5-axis milling device in addition to the three spatial dimensions and the rotatable tension bridge (4th axis), gives the possibility of rotating the 40 milling spindle (5th axis), which enables the milling of complex geometries with subsections. Example in the Laboratory Area: Everest Engine (KaVo). Example in the Production Centre: HSC Milling Device (etkon). The authors stated that the increase of the number of axes does not increase the quality of the restoration which is much more dependent on the result of the digitalization, data processing and production process. Depending on the location of the components of the CAD/CAM systems the production can either be chairside, laboratory or centralized fabrication in a production center. The processing can be dry (i.e. Wieland Zeno, LAVA and Cercon smart ceramics) or wet (i.e. KaVo Everest, Zeno 8060, inLab (Sirona), E4D). The materials that can be milled with those devices are metals (titanium, titanium alloys and chrome cobalt alloys), resin materials, silica-based ceramics (especially lithium-disilicate ceramic blocks that are used for fully anatomical crowns, copings for crowns and anterior FPD frameworks), infiltration ceramics (Vita In-Ceram Alumina, Vita In-Ceram Zirconia, VITA In-Ceram Spinell) and aluminum and yttrium-stabilized zirconium oxide blocks (56). The CAD/CAM technology can be used either with fully sintered ceramic blocks (hard machining) or with partially sintered ceramics (soft machining) (65). Lithium disilicate (IPS e.max CAD) along with feldspar-based and leucite-based ceramics are the available materials used in hard machining, while partially-sintered zirconia blocks are used in soft machining. 41 McLaren and Terry reported that the poor marginal and internal fit of the restorations produced by the early CAD/CAM systems was due to the low resolution scanning and inadequate computing power of those systems and not to the ceramic material itself (71). Shannon et al. compared the absolute horizontal marginal adaptation of zirconium-dioxide cores fabricated from seven contemporary dental laboratory based CAD/CAM systems: KaVo Everest, Nobel Biocare Procera (MOD40, Piccolo, Forte scanners), 3M ESPE Lava, Wieland Zeno and Sirona CEREC (112). Standardized digital photographs were taken of each core on the master die. All the systems except one resulted in cores that were over-extended requiring adjunctive procedures to produce acceptable all-ceramic crowns, which showed that there was area for further improvement. Tan et al. evaluated the vertical marginal openings of the cast, CAM and CAD/CAM restorations (27). The last were titanium restorations fabricated using the scanning and crown design modules of the KaVo Everest system. The CAM titanium restorations were made with the double-scan technique with the same system, and the cast restorations were fabricated using the conventional lost wax technique. Digital photos were taken and the openings were measured with digital software program. The results revealed the following mean vertical openings: CAD/CAM 79.43µm, CAM 73.12µm, cast 23.91µm. This study found that the cast restorations had a significantly smaller vertical marginal gap compared to both the CAD/CAM and CAM groups. Lee et al. evaluated the marginal and internal fit of all-ceramic crowns manufactured by conventional double-layer type (high-strength core) CAD/CAM system 42 (Procera) versus single layer type (without a core-the entire crown is CAD/CAMed) system (Cerec 3D) (70). For the marginal fit, the crowns and the copings were positioned on the die and the distance between each crown’s margin and the metal die was recorded at 50 points randomly using a measuring microscope at a magnification of 100x. For the internal fit, silicon paste was used and the gap was calculated based on the surface area of the abutment and the weight and density of the silicone material. Comparing separately the coping and the crown for the first system, it was shown that the marginal discrepancy width of Procera crowns (89.6±9.5 µm) after porcelain firing showed significantly larger gaps than Procera copings (72.2±7.0 µm). This was attributed to the negative effect of double-layer type CAD/CAM system where porcelain was added to the coping. There was no statistically significant difference in maginal fit between Procera and Cerec 3D, but there was difference in the internal fit. Nevertheless both systems produced crowns within the clinically acceptable range. An important aspect/limitation of the CAD/CAM restorations that needs to be mentioned is the potential for machining-induced damaged and more specifically marginal chipping. Tsitrou et al. conducted a study in order to investigate any possible correlation between the brittleness index (BI) of machinable dental materials and the chipping factor (CF) of the final restorations (113). The BI is the machinability of a material that can be simply assessed qualitatively as the ease with which a given material is cut. The CF is an estimation of the degree of marginal chipping, which can be derived by estimating the ratio of overall marginal chipping over the total marginal circumference of the restoration multiplied by 100. The materials tested were Paradigm MZ100TM (3M/ESPE), Vita Mark II (VITA), ProCAD (Ivoclar-Vivadent) and IPS e.max CAD 43 (Ivoclar-Vivadent) milled in CEREC system (Sirona Dental Systems Gmbh, Bensheim, Germany). Tsitrou et al. showed that the CF increases as the BI increases, with Paradigm MZ100 having the lowest BI and CF, and IPS e.max CAD demonstrating the highest BI and CF. Following on from that study, Giannetopoulos et al. investigated whether the CAD/CAM system would influence the marginal integrity of IPS e.max CAD copings made with different finishing lines (114). Cerec inEOS system/Cerec 3D software and KAVO Everest system were compared. The finish lines were 60-degree bevel, 30-degree bevel and 0-degree bevel (or 90-degree shoulder). The CF was calculated, as in the above study(113); the CF was increased as the bevel angle increased for Cerec group, whereas this factor did not influence the marginal integrity for the Everest group. The conclusions of the study were that different milling processes produce different amount of marginal chipping, although that was apparent only for the 60-degree bevel. Since the introduction of CAD/CAM technology in dentistry, more than 20 years ago, much advancement has been made regarding the milling process of dental prosthesis. Research on this field is dynamic at this point of time, and new technologies with new potentials are introduced continuously. Stereolithography (SLA) Stereolithography (SLA), a typical modality of rapid prototyping (RP), is a relatively new technology that produces physical models from computer-aided design via 3D printers (115). Out of all the RP technologies available, the most commonly used for medical applications are stereolithography and 3D printing (3DP). Stereolithography is a 44 process by which liquid UV-sensitive resin contained in a vat is polymerized via UVlaserbeam into solid resin, which comprises both the 3D model and support pillars, which help maintain the structural integrity as the model is being created. The model is then rinsed in alcohol and again polymerized in a UV chamber. Afterward, the support pillars must be separated from the model by hand. Although RP has been used in the field of industrial design for decades, only in the last decade have applications been developed for medicine and dentistry. This is attributable to the advantages that the technology provides which include fast model fabrication, the ability to use various raw materials and diverse finishing techniques, and the capacity to produce complex 3-D models (115). Those models already serve in the diagnosis, treatment and operation planning in oral and maxillofacial surgery including malformations, craniofacial surgery, tumor surgery, traumatology, orthognathic surgery and implantology (116). Even more recently, this type of model was incorporated in fixed prosthodontics with the introduction of the digital impressions. Lava™ C.O.S. (Lava™ Chairside Oral Scanner, 3M Lexington, USA) works with stereolithography apparatus (SLA) technology to produce the models derived from its data. Ogledzki et al. compared the accuracy of full-arch dental models from a digital intra-oral scanning system, the Lava™ C.O.S. to those produced using traditional 3M PVS impression materials cast in type III stone (117). The SLA and the stone models were scanned using the Lava desktop model scanner to produce digital image representations of the casts. Geomagic Studio (Geomagic, Research Triangle Park, NC, USA) graphical analysis software compared the digital images of the casts to digital reference images provided by an independent agent who conducted a laser scan of the 45 original metal models. The results showed no significant difference in models produced using either the intra-oral scanning system or traditional PVS materials. Moore et al. determined to what extent the micro layer steps resulting from the additive process of SLA models were transferred to the margins of copings used in metalceramic crowns (118). Nine cast chrome-cobalt prepared dies were scanned using the Lava™ C.O.S.; SLA models were created and metal copings were manufactured. Four locations per coping were randomly selected and microscopic photographs were taken of the SLA model and coping interface. The margins of the chrome cobalt teeth preparations, SLA models and copings were digitally traced on the micrographs. It was found that 25 of a total of 36 margins locations had no steps in the SLA model. Only three of those locations were the steps transferred to the metal coping, which gave a stepped appearance. The remaining transfers showed slight transfer to the copings with rounded wave-like appearance. The authors concluded that there was only a very slight correlation (approximately 8%) between the micro layers on the SLA models and marginal characteristics. Digital dental impressions The CAD/CAM technology has been used in dentistry for several years, but its application was limited mainly to the dental laboratory environment. The first clinical use of this technology was introduced more than 20 years ago, when the first intraoral scanner was created. Today, technical evolution is rapid with new scanners for dental impressions entering the common clinical practice continuously. The claimed advantages of digital impressions which may make the conventional impression technique obsolete 46 include accuracy of fit, time and cost savings, lab and dentist benefits and improved communication as well as patients’ comfort and acceptability. A concise and comparative table with all the current intraoral scanners is presented in Logozzo’s et al. and Scotti’s et al. studies (Table 2) (54, 55). The scanners that are not included in that table are the new models of 3M ESPE (i.e. True Definition Scanner) and of Sirona Dental System (i.e. CEREC® AC- Omnicam). For this study, two of those scanners were used; the Lava™ C.O.S. and the E4D. A basic description of the science behind those two scanners will follow as presented on the manufacturer’s brochures and on-line sites as well as scientific papers: Company Sirona Dental System GmbH (DE) Cadent Inc (IL) D4D Technologies, LLC (US) 3M ESPE (US) IOS Technologies, Inc (US) Densys Ltd (IL) Dimensional Photonics International, Inc (US) MHT SpA (IT)-MHT Optic Research AG (CH) Hint-Els GmbH (DE) 3Shape A/S (DK) Intraoral scanner CEREC®ACBluecam iTero E4D Lava™ C.O.S. IOS FastScan MIA3d DPI-3D 3D Progress directScan Trios Confocal microscopy Stereoscopic vision Confocal microscopy and MoireÌ© effect Accordion fringe interferometry (AFI) Active stereophotogrammetry Active triangulation and Schleimpflug principle Active wavefront sampling Optical coherence tomography and confocal microscopy Parallel confocal microscopy Active triangulation and optical microscopy Working principles Not disclosed Not disclosed Not disclosed Wavelength 350500 nm Visible light Laser Pulsating visible blue light Laser Red laser Visible blue light Light source Multiple images Multiple images 3 images Multiple images 2 images 3 images Video Multiple Multiple Multiple images Imaging type None Not discloses Occasional None Yes Yes Yes Occasional None Yes Coating No No No No No No No Yes No Yes In-office milling Proprietary or STL Not disclosed STL STL ASCII STL Proprietary Proprietary Proprietary or selective STL Proprietary Output format 47 48 Lava™ C.O.S. The Lava™ Chairside Oral Scanner (C.O.S.) was created at Brontes Technologies in Lexington, Massachusetts, and was acquired by 3M ESPE (St. Paul, MN). The Lava™ C.O.S. system consists of a mobile cart containing a central processing unit (CPU), a touch screen display, and a scanning wand (Figure 2) (61). Figure 2. The Lava™ C.O.S. system (54). The method used for capturing 3D impressions involves Active Wavefront Sampling. Active (optical) wavefront sampling refers to getting three dimensional 49 information from the Lava™ C.O.S. proprietary single-lens imaging system by measuring depth based on the defocus of the primary optical system (119). In order to understand the concept, the manufacturers refer to classical photography; changing the aperture size, the focal range changes too: a small aperture results in a larger focal range, whereas if the aperture is large the objects in focus are mainly those on the focal plane while all other points become fuzzy. When two wide apertures are used instead of only one, the object point is virtualized as two points, encoding the depth information by means of the image disparity. Image disparity gives the system the Z information to calculate 3D data. Similarly, the Lava™ C.O.S. system provides an active three-dimensional imaging system that includes an off-axis rotating aperture element placed either in the illumination path or in the imaging path of an optical apparatus (120). The system includes a lens 140, a rotating aperture element 160 and an image plane 18A, (Figure 3). 50 Figure 3. The rotation of the aperture mechanism (120). The single aperture avoids overlapping of images from different object regions hence it increases spatial resolution. The rotating aperture allows taking images at several aperture positions and this can be interpreted as having several cameras with different viewpoints, which generally increases measurement sensitivity. The aperture movement makes it possible to record on a CCD element a single exposed image at different aperture locations. To process the image, localized cross-correlation can be applied to reveal image disparity between image frames. As shown in Figure 3, at least two image recordings on the image plane 18A at different angles of rotation of the aperture 160 are used to generate the measured displacement for target object 8A. The separate images are 51 captured successively as the aperture rotates to position #1 at time t and position #2 at time t+At. The rotation center of the image gives the in-plane object coordinates as follows (Figure 4) (120): Figure 4. In-plane object coordinates. where X0,Y0 , are the in-plane object coordinates, f is the focal length of the lens objective, L is the depth of in-focus object points (focal plane), R is the radius of the circle along which the off-axis pupil is rotating, and d is the diameter of a circle along which the relevant out-of-focus point is moving on the image plane 18A as the aperture is rotated. The magnitude of the pattern movement represents the depth information (Z0) measured from the lens plane. Z0, can be evaluated from two Snell’s lens laws for infocus and out-of-focus object points and by using similar triangles at the image side (Figure 5) (120): Figure 5. The depth information. 52 This method enables “3-D video in Motion” which means that Lava™ C.O.S. is able to capture approximately 20 3D data sets per second, or close to 2,400 data sets per arch. A series of 5 slides, provided by the 3M ESPE Product Marketing Manager, illustrates the concept of measuring out-of-plane coordinates of object points by sampling the optical wavefront, with an off-axis rotating aperture element, and measuring the defocus blur diameter, (Figure 6): Figure 6. The Active Waveform Sampling. Courtesy of Todd W. Deckard. 53 Figure 6. Continued. 54 Figure 6. Continued. 55 After the preparation of the tooth and gingival retraction, the entire arch is dried and lightly dusted with powder to locate reference points for the scanner. During the scan, a pulsating blue light emanates from the wand head and an on-screen image of the teeth appears instantaneously (54). Once the data have been received, the digital prescription is sent to the laboratory where by the use of specific software, they digitally cut the die and mark the margin. 3M ESPE receives the digital file, generates a stereolithography (SLA) model and sends it to the laboratory. When the lab receives the SLA model, they can create a conventional restoration such as PFM, gold, glass-ceramic, or CAD/CAM LAVA™ all-ceramic. Accuracy of Lava™ C.O.S. Ender and Mehl compared the accuracy (trueness and precision) of digital impressions of the full arch with that of conventional impressions on an in-vitro model (121). The master model was acquired, with a reference scanning process, with known measuring trueness and precision. Plaster models of this model made of conventional impressions were produced and then digitized with the same reference scanning process. Also, digital impressions were made with the Cerec AC Bluecam and the Lava™ C.O.S.. The data records were superimposed and the deviations from the master model defined the trueness of the impression method. Their results showed that the accuracy of the digital impressions was similar to that of the conventional impression. In another study, the most accurate scanner with the scanning protocol that would ensure the most accurate digital impression for implant dentistry was studied (122). A 56 master model made of stone was fitted with three high precision manufactured PEEK (polyether-ether-ketone) cylinders and scanned with three intra-oral scanners: the CEREC (Sirona), the iTero (Cadent) and the Lava™ C.O.S (3M). The digital files were imported in software and the distance between the centers of the cylinders and the angulation between the cylinders was assessed. The Lava™ C.O.S. had the smallest and most consistent distance errors, while the CEREC had the largest and most inconsistent. The accuracy of this system was assessed again, this time with regard to direct and indirect digitalization (123). More specifically, test datasets were generated in vitro 1) with the Lava™ C.O.S. 2) by digitizing polyether impressions and 3) by scanning the referring gypsum cast by the Lava Scan ST (3M ESPE, Seefeld, Germany) laboratory scanner. Using inspection software (Qualify 12.0, Geomagic, Research Triangle Park, NC, USA), these datasets were superimposed by a best-fit algorithm with the reference dataset (REF), gained from industrial computed tomography, and divergences were analyzed. The direct digitalization with Lava C.O.S. showed statistically significantly higher accuracy compared to the conventional procedure of impression taking and indirect digitalization. Repeatability of Lava™ C.O.S. Balakrishnama et al. determined the dimensional repeatability from the Lava™ C.O.S. over a single prepared tooth from a single scanning system over a two-week period of time (124). According to the investigators, the repeatability should determine the system's capability for capturing the proper location and dimensions of the margin of the prepared tooth. Copper dies were scanned with the Lava C.O.S. system 25 times 57 during a period of two weeks. Geomagic (Research Triangle Park, NC, USA) software was used to compare each of the scans, using the original scan as a reference for every comparison, in order to determine the dimensional repeatability from the system. The results revealed that the anterior repeatability was ≤5.8 µm, and posterior repeatability ≤10.9 µm, so the authors concluded that this scanner demonstrated a dimensional repeatability for single preparations that is beyond the requirement to achieve clinical acceptability for marginal adaptation in a crown or bridge restoration (normally accepted to be 50 to 100 µm). Fit of crowns made from Lava™ C.O.S. impressions Lava™ C.O.S. is a system that has attracted the attention of the researchers and thus it has been much studied since its introduction to clinical practice. Syrek et al. compared the fit of all-ceramic crowns fabricated from intraoral digital impressions with the fit of all-ceramic crowns fabricated from PVS impressions (58). Two LavaTM (zirconia) crowns were fabricated for the same preparation in each of 20 patients. One crown was fabricated using the Lava™ C.O.S. and the other crown from the PVS impression material. Prior to cementation, two calibrated and blinded examiners clinically evaluated the fit of both crowns. The marginal fit was also scored using the replica technique. The results revealed significant difference between the two groups; allceramic crowns resulting from Lava™ C.O.S. demonstrated significantly better marginal fit than all-ceramic crowns fabricated from conventional impressions (both groups were within clinically acceptability). 58 Scotti et al. published a very similar paper to the above, where they measured the marginal and internal fit of zirconia crowns made from Lava™ C.O.S. using the replica technique (55). Four different points were evaluated using stereomicroscopy with a magnification of 50x. The results showed that all values were clinically acceptable and the authors considered this method to be accurate enough to be used as an alternative to conventional impressions. Seelbach et al. compared the accuracy of all-ceramic crowns obtained from intraoral scans with Lava™ C.O.S. (3M ESPE), CEREC (Sirona), and iTero (Straumann) with conventional impression techniques (two-step and single-step putty-wash impressions) (62). For both conventional techniques 10 crowns were made of two materials (Lava zirconia (3M ESPE, USA) and Cera E cast crowns (Elephant Dental B.V., The Netherlands)). Then, 10 digital impressions (Lava C.O.S.) were taken and Lava zirconia (3M ESPE, USA) crowns were manufactured, 10 all-ceramic crowns were fabricated with CEREC (Empress CAD, Ivoclar Vivadent, Liechtenstein) and 10 allceramic crowns were made with iTero (Copran Zr-I, White Peaks Dental Systems, Germany). Measurements were performed with a 3D-coordinate measuring system (CNC Rapid, Thome Präzision GmbH, Germany). The deviation was assessed at 50 points per crown (3D measuring precision, 3µm in this setup). Metrolog XG (Version 12.003 HF1, Metrologic Group S.A., France) was used as controlling software. Both, marginal and internal fit, were assessed. With regard to internal fit, the best results were obtained for Lava zirconia restorations made on the basis of Lava™ C.O.S. scans, while the biggest deviations were found for CEREC manufactured Empress CAD crowns. Both differed statistically from the other groups. With regard to marginal fit, CEREC crowns neither 59 differed statistically significantly from Lava™ C.O.S. crowns nor from crowns made on the basis of single-step putty-wash impressions. The internal and marginal fit of the zirconia copings generated from scanning a definitive cast made from a conventional impression and copings generated from a digital impression system were evaluated, in a Master’s thesis, by Hirayama et al. (125). Specimens were divided into 2 groups; group A: 18 Zirconia copings fabricated by scanning stone models from PVS impressions and group B: 19 Zirconia copings fabricated by scanning the master cast and transmitting the scanned data to the Lava™ milling center in St. Paul, MN. To evaluate the fit, the authors used the replica technique described by Boening et al (32) and Molin & Karlsson (31). They found a statistically significant difference between groups A and B, with group B having a better marginal adaptation. That study concluded that both groups had clinically acceptable marginal fit; however the ones made from the digital impression had significantly smaller marginal discrepancies. E4D Dentist System™ The E4D Dentist System™ (D4D Technologies LLC, Richardson, TX) was introduced in early 2008. It consists of a cart containing the design center (computer and monitor) and laser scanner, a separate milling unit (center), and a job server and router for communication (Figure 7) (61). 60 Figure 7. The E4D Dentist System™ (54). According to the company’s brochure, the E4D is the only CAD/CAM system that uses a true laser for scanning hard and soft dental tissue, impression material, occlusal registration material and dental stone. It combines laser technology with micromirrors developed exclusively for D4D technologies by Texas Instruments. The mirror system allows a single laser point to be generated across a large area in a patterned approach thereby eliminating any speckling or diffusion of the laser point. This laser does not require a contrast agent. The working principle of this system is called optical coherence tomography and confocal microscopy. Optical coherence tomography (OCT) is analogous to ultrasound 61 imaging, except that the imaging is performed using light rather than sound (126). By measuring the echo time delay and intensity of back-reflected light, OCT can reveal tissue microstructure with micron-level resolution; image resolutions of 1–15 µm, one to two orders of magnitude finer than standard ultrasound (127). The axial resolution of OCT is determined by the coherence length of the light source: Dz = (2ln2/pi (pi=3.14159)) (l2/Dl), where Dl is the full-width-at-half-maximum of the source spectrum (the difference between the two wavelength values at which the power is equal to half of its maximum value) and l is the center wavelength of the source spectrum (128). Confocal microscopy increases optical resolution and contrast; it enables the reconstruction of three dimensional (3D) structures by stacking individual 2D en-face images at different depths (126). The laser digitizer includes a laser source coupled to a fiber optic cable, a coupler and a detector (54). The coupler splits the light from the light source into two paths. The first path leads to the imaging optics, which focuses the beam onto a scanner mirror, which steers the light to the surface of the prepared tooth. The second path of light from the light source via the coupler is coupled to the optical delay line and to the reflector; this reference path is of a controlled and known path length, as configured by the parameters of the optical delay line. Light is reflected from the surface of the object, returned via the scanner mirror and combined by the coupler with the reference path light from the optical delay line. The combined light is coupled to an imaging system and imaging optics via a fiber optic cable. By utilizing a low coherence light source and varying the reference path by a known variation, the laser digitizer provides an Optical Coherence Tomography (OCT) sensor. The focusing optics is placed on a positioning 62 device in order to alter the focusing position of the laser beam and to operate as a confocal sensor (129). A series of imaged laser segments on the object from a single sample position interlace between two or multiple 3D maps of the sample from essentially the same sample position. The time period to measure each interlaced 3D map is reduced to a short interval and relative motion effects between the intra-oral device and the patient are reduced. The interlaced 3D maps may be aligned with software to produce an effective single view dense 3D point cloud that has no motion induced inaccuracies or artifacts. The motion of the operator between each subframe may be tracked mathematically through reference points in the dataset itself. The operator motion is removed in subsequent analysis (129). The scanner must be held a specific distance from the surface being scanned; this is achieved with the help of rubber-tipped “boots” that extend from the head of the scanner (60). Placing these rests on adjacent teeth steadies the scanner at this optimal distance. The ICEverything™ feature of the E4D takes actual pictures of the teeth and gingiva. A diagram on the monitor shows the user how to orient the scanner to obtain the next image. As successive pictures are taken, they are wrapped around the 3D model to create the ICEverything model. Once captured the 3D data are displayed as virtual model. The software allows the operators to design as many as 16 units at once and switch back and forth to select any tooth to alter the design. The milling center communicates wirelessly with the design center so that the finished restoration can be milled in the office or remotely (60). 63 Accuracy of E4D Dentist System™ Plourde et al. evaluated in vitro the marginal and internal fit of all-ceramic crowns made with E4D Dentist System™ (63). All monolithic lithium disilicate crowns (IPS e.max® CAD LT A1/C14) were cemented under constant pressure, embedded in acrylic and then sectioned facial-lingually. The sections were evaluated under microscope in 3 locations (marginal-edge, mid-axial wall, and cusp-tip). The results showed that the mean fits ranged from 66.37 µm to 207.61 µm depending on location of measurement. The marginal gap on the facial was 71.96 ± 62.19µm and on the lingual 149.11 ± 67.93µm. They concluded that the marginal and internal fits fell within the range of what several previous studies considered to be clinically acceptable and that the large standard deviations suggest a high degree of variability in scanning, designing, and milling capabilities. In another study, by Kugel et al., tooth #14 was prepared per standard specification to receive an all-ceramic crown restoration on typodont (130). All-ceramic CAD/CAM crowns (Group 1) were fabricated with the E4D Dentist System™. For press laboratory-made crowns, impressions were taken with two-step impression techniques using a custom triple tray with light and putty consistency VPS. Impressions were sent to two dental laboratories (Group 2 and 3) for fabricating the monolithic press lithium disilicate crown. All crowns were cemented using Multilink® Automix (Ivoclar Vivadent) under constant pressure of 100 N, then they were embedded in acrylic and sectioned facial-lingually. Sections were evaluated under digital microscope and measured on three locations per facial and lingual side of section: marginal edge, midaxial wall, and cusp tip. The results showed no significant difference at the facial (Group 64 1: 44.88±32.06, Group 2: 33.46±38.77 and Group 3: 45.22±41.87) and lingual (Group 1: 39.07±32.44, Group 2: 36.09±38.47and Group 3: 62.02±49.96) margins in all groups. However, there was significant difference for cement thickness in midaxial, cusp and occlusal within the group. The authors concluded that there was no statistical difference in marginal fit of all-ceramic crowns made by CAD/CAM system or laboratory press ceramic. Summary-Purpose With the evolving technology and the incorporation of new materials in restorative dentistry, research is mandatory in order to indicate which ideas are appropriate for clinical use and support the need for further research. The purpose of this study was to evaluate the fit of one of the most currently used restorative material (lithium disilicate- press and CAD) made from two different impression techniques (PVS and Lava™ C.O.S.). 65 CHAPTER III MATERIALS AND METHODS Introduction The marginal and internal adaptation of IPS e.max crowns made from two impression techniques and two fabrication methods were evaluated. The impression techniques evaluated in this study were a conventional impression using polyvinyl siloxane material and custom tray, and a digital impression using Lava™ C.O.S. The crown fabrication methods used in this study were the hot-pressed and the computeraided design/computer-aided manufactured (CAD/CAM). Four groups were created: Group A: conventional impression/IPS e.max Press, Group B: conventional impression/IPS e.max CAD, Group C: digital impression/IPS e.max Press, and Group D: digital impression/IPS e.max CAD. The dies and the crowns were digitized using a laser scanner and the adaptation was measured using sophisticated software. Pilot study A pilot study was conducted prior to the main study in order to test the proposed protocol and conduct a power analysis (Figure 8). A brief description of the materials and methods used as well as the results will be presented at the beginning of this chapter prior to the definitive study. 66 Master Die 10 PVS Impressions Group A 5 IPS e.max Press crowns Group B 5 IPS e.max CAD crowns 10 Lava™ C.O.S. Impressions Group C 5 IPS e.max Press crowns Group D 5 IPS e.max CAD crowns Figure 8. Study Groups for the pilot study. A dentaform replica mandibular first molar tooth number thirty (#30) (Dentaform, Frenso, CA, USA) was prepared for an all-ceramic crown. Ten (10) conventional impressions were made with, light and heavy body polyvinyl siloxanes (Extrude Type 3low consistency; Extrude Type 1-high consistency, Kerr Dental, Orange, CA) and a custom tray (SternTek, Sterngold Restorative Systems, Attlebono, MA, USA). The impressions were poured with Type IV gypsum (Resinrock, Whip Mix Corp, Louisville, KY, USA). Ten (10) digital impressions were made using the Lava™ C.O.S. (Lava™ Chairside Oral Scanner, 3M Lexington, USA) and SLA resin casts ((In’Tech, Minessota, MN, USA) were produced. Five (5) dies from each impression technique were used to fabricate the pressed crowns and five (5) to fabricate the CAD crowns. For the pressed crowns, one (1) coat of die spacer (Rem-e-die, Ivoclar Vivadent, Schaan, Liechtenstein) was used to within 1mm of the margin. A full contoured crown was waxed up, invested and pressed using IPS e.max Press (IPS e.max Press HT A1, Ivoclar Vivadent, Schaan, 67 Liechtenstein). The intaglio of the crown was adjusted twice, if needed in order to improve the fit, simulating clinical conditions. For the CAD crowns, the dies were scanned using the Kavo Everest ScanPro (Kavo Dental GmbH, Biberach, DE), a noncontact 3D strip-light projection scanner. One (1) coat of spray (Occlude® The Indicator, Pascal Int. Co, Bellevue, WA) was applied prior to scanning. The full contoured crowns were designed with 55µm die spacer and 10µm marginal horizontal overextension. IPS e.max CAD blocks (IPS e.max CAD HT Block I12 A1, Ivoclar Vivadent, Schaan, Liechtenstein) were milled using the Kavo Everest milling engine. The intaglio of the crowns was adjusted twice as with the pressed crowns and then they were crystallized. The intaglio of all the crowns and the master die were digitized using the same Kavo scanner. A data set was created for the Qualify 2012 software (Geomagic, Research Triangle Park, NC, USA). The intaglio of the each crown and the master die were manually aligned first and then further refined by using the software’s Best-Fit Alignment. Sections were made in facial-lingual (FL) and mesio-distal (MD) directions. Five (5) points in each section were measured (2 on the margins, 2 in the middle of the axial walls and 1 on the middle of the occlusal surface); a total of ten (10) points per crown were analyzed (margin-M, margin-D, margin-F, margin-L, mid-M, mid-D, mid-F and mid-L, occlusal-MD and occlusal-FL). A two-sample t-test and the nonparametric Wilcoxon rank-sum test (i.e. when the assumption of normality was violated) were used to detect a difference in the marginal and internal gap values between two impression techniques and between two crown fabrication methods. One-way ANOVA with post-hoc Tukey’s HSD test was conducted to determine whether there was a significant difference in the marginal gap values among 68 the four experimental groups. The Shapiro-Wilk test was applied to verify the assumption of normality. If the assumption of normality was violated, one-way ANOVA to the ranked data followed by post-hoc Bonferroni multiple comparison test, an equivalent test statistic to the nonparametric Kruskal-Wallis test, was used as the test statistic. Results of the pilot study The results showed that when the impression techniques were compared: between Group A vs. Group C (pressed crowns) the mean marginal gap values for digital impression were significantly greater than those using the conventional impression for two variables (mid-M and margin-M). No statistical difference was found for the remaining 8 variables. Between Group B vs. Group D (CAD crowns) the mean marginal gap values for digital impression were significantly greater than those using the conventional impression for 4 variables (mid-F, occlusal-FL, mid-M, margin-M). No statistical difference was found for the remaining 6 variables (Table 3). When the crown fabrication techniques were compared, between Group A vs. Group B (conventional impression) the mean marginal gap values for CAD crowns were significantly greater than the pressed crowns for two variables (occlusal-MD and occlusal-FL). No statistical difference was found for the remaining 8 variables. Between Group C vs. Group D (Digital impression) the mean marginal gap values for CAD crowns were significantly greater than those using the conventional impression for four variables (margin-F, mid-F, occlusal-FL, occlusal-MD, margin-L). No statistical difference was found for the remaining five variables (Table 3). N 5 5 5 5 Experiment Groups Conventional/press Conventional/CAD Lava/press Lava/CAD 0.187 (0.05) 0.087 (0.05) 0.151 (0.06) 0.152 (0.09) Mean Avg margi n-F in mm (SD) 0.200 (0.05) 0.121 (0.01) 0.130 (0.02) 0.113 (0.04) Mean Avg mid-F in mm (SD) 0.331 (0.06) 0.164 (0.08) 0.224 (0.04) 0.126 (0.02) Mean Avg occlusal -FL in mm (SD) 0.128 (0.04) 0.133 (0.04) 0.080 (0.03) 0.080 (0.06) Mean Avg mid-L in mm (SD) 0.128 (15.00) 0.030 (5.00) 0.120 (13.80) 0.056 (8.20) Mean Avg marginL in mm (mean rank scores) 0.097 (0.03) 0.122 (0.07) 0.104 (0.08) 0.046 (0.03) Mean Avg margi n-D in mm (SD) 0.145 (0.02) 0.165 (0.05) 0.159 (0.04) 0.114 (0.04) Mean Avg mid-D in mm (SD) 0.323 (0.07) 0.169 (0.04) 0.255 (0.03) 0.157 (0.02) Mean Avg occlusal -MD in mm (SD) 0.178 (0.03) 0.128 (0.04) 0.045 (0.02) 0.046 (0.04) Mean Avg mid-M in mm (SD) 0.132 (0.04) 0.099 (0.03) 0.053 (0.01) 0.037 (0.02) Mean Avg margi n-M in mm (SD) 69 70 Intraclass correlation coefficients were also computed to assess intra-observer agreement in tracing of marginal and internal gap between the two duplicate measurements of all variables. Overall, there was very strong evidence that these intraclass correlations differed from zero (p<0.001 for each instance), and the correlation coefficients indicated from a moderate to a strong agreement between the two measurements made by the single observer. Based on the pilot data, the sample size per group that was required to distinguish statistical difference among the four groups with 80% (or 90%) power for each of the 10 variables was calculated. Validation of the Digital Measurement Technique Since the digital measurement is a new method of evaluating the fit in prosthetic dentistry, in order to validate it, it was compared to the classic replica technique. Light body Extrude material was injected in the intaglio surface of five (5) IPS e.max CAD crowns. The crowns were seated on the master die and held for 10 minutes with finger pressure. After the material set the crown was removed and the PVS material was sectioned with a blade at the same reference points as were the digital sections. The sectioned PVS material was placed on a glass slide with PVS adhesive parallel to horizontal plane. A Zeiss Axiotech Microscope (X50) (Carl Zeiss Microscopy, LLC, Thornwood, NY, USA) was used to make the digital photographs and the NIH-ImageJ software (NIH, ImageJ 1.46) was used to measure the thickness. A paired-sample t-test was used to determine whether a significant difference in marginal gap existed between 71 the two techniques (replica vs. digital). A p-value of less than 0.05 was used as a criterion for statistical significance. Based on the paired-sample t-test, there was no significant difference in marginal and internal gap between the two measurement techniques (p>0.05 for each instance) for most of the points tested. Out of ten measurements, three cases showed that mean marginal gap values were significantly greater for the replica technique than those measured using digital technique; occlusal-MD (p=0.0122), occlusal-FL (p=0.0709) and mid-M (p=0.0032). After the completion of the pilot study, the following changes were made to the protocol for the definitive study: 1. Fifteen (15) samples per study group would be included. 2. Low translucency (LT) ingots instead of high translucency (HT) would be used to facilitate scanning of the crowns. 3. No adjustments in the intaglio of the crowns would be performed in order to adjust the fit since the standardization of this procedure was difficult and subjective. 4. A chairside scanner and milling engine was selected for the fabrication of the CAD crowns (E4D vs. Kavo). 5. A different scanner would be used in order to digitize the specimens for the measurements from the one used to fabricate the CAD crowns, in order to avoid errors related to the scanner. 72 6. A more detailed protocol would be developed to align the crown and master die. After Best-Fit Alignment, manual alignment would be done until the most realistic fit was obtained. 7. Different statistical analysis would be performed; the average mean marginal and internal gap would be assessed instead of the individual points in each section. 8. The 3D marginal fit would be calculated as well. Definitive study A thorough description of the materials and methods used in the definitive study will be discussed in this chapter (Figure 9). Master Die 30 PVS Impressions Group A 15 IPS e.max Press crowns Group B 15 IPS e.max CAD crowns Figure 9. Study Groups for the definitive study. 30 Lava™ C.O.S. Impressions Group C 15 IPS e.max Press crowns Group D 15 IPS e.max CAD crowns 73 Study design Master die fabrication A full dentate dentaform (Dentaform, Frenso, CA, USA) with teeth numbers 1-32 was used. A dentaform replica mandibular first molar tooth number thirty (#30) was prepared for an all-ceramic crown by a single operator. High-speed hand piece (Brasseler, Savabbah, GA, USA) was used to prepare the tooth according to standard tooth preparation procedures. The prepared margin of the tooth was clearly defined by a distinct heavy chamfer made with diamond bur (No. 6856SU, Brasseler, Savabbah, GA, USA). The circumferential reduction of tooth substance was 1.5 mm. The occlusal reduction was 1.5 mm. All internal line and point angles were rounded. The convergence angle of the prepared tooth was at 5-10°. The margin was placed 1mm above the gingiva (Figure 10, 11). Figure 10. Master die frontal view. 74 Figure 11. Master die occlusal view. Impression making Two impression techniques, conventional impression, and digital impression with Lava™ C.O.S were used in the study. These are described separately below. Conventional impression Custom trays extended only in the mandibular right quadrant were fabricated as follows: two layers of baseplate wax (Shurwax, Modern Materials, Heraeus Kulzer, LLC IN, USA) were applied on the dentaform to create approximately 2mm of uniform space for custom tray fabrication. The wax extended from tooth #26 to #32, 3mm below the CEJ. Two tissue stops were created on #29 and 31 by removing wax until the tooth was seen. An aluminum foil separator was used to cover the wax. One layer of lightpolymerized resin (SternTek, Sterngold Restorative Systems, Attlebono, MA, USA) was 75 adapted to the foil covered wax spacer. The resin was extended from the distal of #32 to tooth #24 in direct contact with #24 and #25 for stability. It was cured in the visible lightpolymerizing unit (Triad 2000, Dentsply, York, PA, USA) for 2 minutes to fabricate the custom tray. The wax and the aluminum foil were carefully removed using a Buffalo knife and the intaglio of the tray was light-cured for 2 more minutes. Custom trays were fabricated 72 hours before the impressions were made to allow them to become dimensionally stable. A thin layer of tray adhesive (Tray Adhesive 16622, Kerr Dental, Orange, CA) was brushed onto the tray 24 hours after the tray fabrication. The adhesive bonds the impression material to the tray, and thus, controls the direction of polymerization shrinkage of the material. All the custom trays were allowed to dry for 48hr after the application of the tray adhesive. Cartridges of polyvinyl siloxane, light and heavy body, (Extrude Type 3-low consistency; Extrude Type 1-high consistency, Kerr Dental, Orange, CA) were loaded individually into dispensing guns. An impression syringe and intraoral tip were attached to dispensing guns. The heavy body was injected into the half arch custom tray. At the same time, the light body material was carefully injected on the prepared tooth (#30) including the sulcus until the tooth was completely covered. The custom tray loaded with heavy body was seated on the dentaform. After 11 minutes from the start of mix, the set impression material/custom tray was removed from the master cast by pulling slowly to break the seal, then snapped out along the long axis of the teeth. The impression was carefully examined under a microscope (10x) to make sure the preparation margin was intact. 76 After 24 hours, each of the 30 PVS impressions was treated with a surface tension reducing agent (Vacufilm; Kerr Dental, Orange, CA) and light compressed air was used to remove the excess. Type IV gypsum (Resinrock, Whip Mix Corp, Louisville, KY, USA) was used to fabricate the definitive casts. Resinrock was mixed with the ratio of 70g powder to 14 ml water. Hand spatulation was performed until all powder was incorporated and then was mechanically spatulated under a vacuum for an additional 30 seconds. Resinrock was carefully poured into the impression using a spatula. After 72 hours the gypsum cast was removed from the impression. The same procedure was repeated 30 times to obtain 30 stone casts (Group A and B). The stone casts were then prepared to receive two pins (Pindex; Coltene/ Whaledent, Mahwah, N.J.) per removable section. Pins were attached with cyano-acrylate cement to the facial and lingual aspect on working dies. Dental stone (Flowstone, Whip Mix Corp, Louisville, KY, USA) was poured around the pins to form a base. After the base was set, the cast was separated and prepared tooth #30 was sectioned using a diamond disk (No. 6924HP400, Brasseler, Savabbah, GA, USA). The die was trimmed under microscope (10x) (Meiji Techno, Model BM 47491) using a carbide bur (No. H78060, Brasseler, Savabbah, GA, USA) and stainless steel surgical blade No.21 (Figure 12). 77 Figure 12. Stone die. Digital impression For the digital impressions, a thin dusting with titanium dioxide powder (3M ESPE, Lexington, USA) was applied to the lower right quadrant of the dentoform to facilitate the scanning of the prepared tooth and the adjacent teeth. The prepared tooth (#30) was scanned with the Lava™ C.O.S. (Lava™ Chairside Oral Scanner, 3M Lexington, USA) intraoral scanner. After verification that the margins were clearly visible, all details of the preparation were captured. Then, the occlusal, facial and lingual surfaces of the quadrant were scanned. The scans were individually checked for contiguous data before their acceptance. The scanning data were sent to the manufacturing center (In’Tech, Minessota, MN, USA). This procedure was repeated 30 times (Group C and D). Thirty (30) resin (SLA) models with removable dies (SLA resin casts, St.Paul, 3M Lexington, USA) were produced (Figure 13). 78 Figure 13. Resin die. IPS e.max Press crown fabrication IPS e.max Press (IPS e.max Press LT A1, Ivoclar Vivadent, Schaan, Liechtenstein) ingots were used to make the full coverage crowns using the lost wax technique. Fifteen (15) stone casts produced by the conventional impression and fifteen (15) resin casts produced by the digital impression were used. The margins were clearly defined by using a blue pencil (Margin liner, Kerr, Orange, CA). One layer of die hardener (Industrial Krazy Glue, Aron Alpha, Type 202, OH, USA) was applied on the stone dies, extending 1 mm occlusal and apical to the margin. One layer of rubber-based removable die spacer (Rem-e-die, Ivoclar Vivadent, Schaan, Liechtenstein) was used on all dies, stone and resin, extending 1 mm occlusal to the margin, as recommended by the laboratory technician of the manufacturer company (Ivoclar Vivadent). The dies were lubricated (DieLube, Ney, Denstply Ceramco, York, PA) and then dipped in hot dipping 79 wax (Bellewax, Kerr, Orange, CA). A wax pattern for the full contoured crown was fabricated using wax addition technique on one of the stone casts. A matrix from PVS material (Exaflex putty and light body Extrude) was made in order to standardize the thickness and the shape of the pressed crowns. A hole was made on the facial of the matrix in order to inject the melted wax (Yeti Dental, Thowax, Eugen, Germany). The matrix was adapted on each cast and the wax was injected. After 1 minute the matrix was removed and the excess wax was cleaned. If there were any irregularities, more wax was added to finish the contours. The margins were carefully finished and checked under the microscope (10x) using margin wax (Almore, INC, Portland, OR, USA) with an electric waxer (Dual Carving Pencil, Whip Mix Corp, Louisville, KY, USA). The wax patterns were sprued at the mesial facial cusp, which was the thickest part of the wax-up, using an 8 gauge wax sprue (Kerr, Orange, CA) 3mm long. The sprues were attached to the IPS investment ring (IPS investment ring System 200g, Ivoclar Vivadent, Schaan, Liechtenstein) base with rope wax and sticky wax, positioning with 45-degree inclination from the center. Four crowns were invested in each investment ring. The investment (IPS PressVEST Speed, Ivoclar Vivadent, Schaan, Liechtenstein) was mixed according to the manufacturer’s instructions. The filled investment ring was allowed to set for 35 min. The ring gauge and the ring base were removed with a turning movement. The investment ring was placed in the preheated furnace (850oC) at the final temperature for 45 min. The cold IPS e.max Press ingot and the IPS Alox Plunger, after it was dipped in IPS Alox Plunger Seperator, were placed into the hot investment ring using metal tongs. The assembled investment ring was placed in the center of the hot press 80 furnace (Programat EP5000, Ivoclar Vivadent, Schaan, Liechtenstein). The recommended pressing program for IPS e.max Press LT was used (Table 4). B t ì T H E o o o C mm:ss µm/Min 917 25:00 250 C 700 C/Min 60 Table 4. Pressing program parameters for IPS e.max Press LT. The ring was left to cool to room temperature for 60 minutes. The length of the plunger was marked on the cooled ring. The investment ring was sectioned using a separating disk. The crowns were divested with Type 50µm Al2O3 (Blasting compound, Ivoclar Vivadent, Schaan, Liechtenstein) at 4bar pressure. After fine divesting, the crowns were immersed in a plastic cup with the IPS e.max Press Invex Liquid (Ivoclar Vivadent, Schaan, Liechtenstein) and placed in an ultrasonic cleaner for 15 minutes in order for the reaction layer to be removed. The restorations were cleaned under running water and then dried. The reaction layer was removed with Type 50µm Al2O3 at 4bar pressure. The sprues were cut using a fine diamond disk (No. 6918BHP220 Brasseler, Savabbah, GA, USA) 1mm away from the actual cusp tip in order to avoid perforation. The sprue attachment area was smoothed and rounded using a polishing disk (No. 94002CHP170 Brasseler, Savabbah, GA, USA). The intaglio of the crowns was observed 81 under the microscope (10x). Any residual positives from the investing procedure were carefully removed with a diamond bur (Brasseler, Savabbah, GA, USA). No further adjustments were made to the intaglio surface. IPS e.max CAD crown fabrication The remaining 15 stone casts and 15 resin models were scanned using the E4D scanner (D4D Technologies, Richardson, TX, USA) and the crowns were designed with the E4D Design Center. Based on the software instructions (Dentalogic TM 4.5.0.34), a new file was created for each die, and the following steps were performed: 1. Setup: Select “Crown,” “Bite Registration,” Library “A,” Material “IPS e.max CAD LT” and Shade “A1.” 2. Scan: The die alone was scanned. 3. Margin: Select “Trace” in order to define the margin. 4. Design: Select “Thickness;” spacer thickness “0.04” and margin ramp “1.00” of the crown. The thickness of the crown was adjusted to be more than 1mm all around. The margin was enhanced in order to avoid chipping during milling, by enabling the default settings under Margin Boost Settings “0.150” and “2.000”. 5. Mill: The sprue was placed in the proximal surfaces as far from the margin as possible. Select “Detail mill.” 6. Data were sent to the E4D milling engine. 82 7. IPS e.max CAD blocks (IPS e.max CAD LT Block I12 A1, Ivoclar Vivadent, Schaan, Liechtenstein) were tightened into the milling engine. After the crowns were milled, the sprues were cut a using diamond bur (No. 6918BHP220 Brasseler, Savabbah, GA, USA). The horizontally overhanging margins were adjusted under microscope (10x) with a polishing disk (No. 94002CHP170 Brasseler, Savabbah, GA, USA). No adjustments on the intaglio surface were made. The intaglio surface of the crowns was filled with IPS Object Fix Putty/Flow (Ivoclar Vivadent, Schaan, Liechtenstein) and then it was placed on a crystallization pin. The crowns were crystallized using a ceramic furnace (Ney Centurion Qex, Denstply Ceramco, NJ, USA). The recommended speed crystallization/Glaze LT program used is presented in Table 5. Representative examples of the crystallized crown intaglio surfaces are depicted in Figure 14. 6:00 403 oC o S B min rate time temperature C/min 90 t1 Heating Closing Stand-by 820 oC T1 temperature Firing min 0:10 H1 time Holding o C/min 30 t2 rate Heating 840 oC T2 temperature Firing min 7:00 H2 time Holding o C o C 820/840 21/22 11/12 550/820 Vacuum 2 Vacuum 1 700 oC L cooling Longterm o C/min 20 tl rate Cooling 83 84 Figure 14. Intaglio of one indicative crown from each experimental group. Measurement of the crown fit The fit of a crown on a tooth refers to the adaptation of the prosthesis on the walls of the prepared tooth. For this study, in order to assess the fit, the gap between the intaglio of the crown and the tooth surfaces was acquired. A small gap means close proximity of the two surfaces, which is interpreted as a good fit. In other words, the smaller the gap, the better the fit, and conversely the bigger the gap, the poorer the fit. A description of the how that gap was calculated in 3D and 2D follows. The master die and the intaglio of each of the crowns were digitized using the Surveyor ZS-Series scanner which is a 3D laser coordinate measurement machine (CMM) with scan accuracy of +/- 0.00898mm (Laser Design Inc, GKS, Minneapolis, 85 MN, USA). A light coat of spray was used to facilitate the scanning (Spotcheck® SKDS2 Non-Halogenated Solvent Developer, Magnaflux, Glenview, IL, USA). Three different scans were made according to the triple-scan protocol by Holst et al. (39): 1) The prepared dentaform tooth (Master Die) secured on a standardized metal base with PVS material, 2) The intaglio of each one of the all-ceramic crowns, and 3) Each crown on the tooth in a clinically correct final position. Digital Shape Scanning and Processing (DSSP) was applied using Geomagic Qualify 2012 software (Geomagic, Research Triangle Park, NC, USA). For each specimen a separate data set in STL format was generated from point clouds with the Geomagic Qualify 2012 software. First, the master die STL file and the crown-master die STL file were registered by manual alignment followed by best-fit registration. Then the same procedure was done to register the crown STL file and crown-master die STL file. The crown-master die STL data set was deleted and the aligned crown to master die STL data set was used for fit assessment. For the 3D measurements, an area above the cavosurface margin with 0.75mm occlusal-gingival width circumferentially was defined using the Geomagic Studio 2012 software as follows (Figure 15): 1. Trace the margin using Draw Curves under the Curves menu. 2. Right Mouse Click>Duplicate the curve in the Model Manager. 3. Tools>Move>Exact Movement to move the duplicated curve 0.75mm above the margin. 4. Project the translated curve down to the polygon surface using Curves>Free Curves>Project 86 5. Convert both curves to boundaries by using Curves>Projected Curves>Convert To 6. Finally, save the model as an independent object Figure 15. Defined area around the margin of the Master Die with 0.75mm occlusalgingival width. For the 2D measurements, two sections, one facial-lingual and one mesial-distal, were made through the grooves on the standardized metal base of the tooth. The distance between the die and the intaglio surface of the crown were measured at 7 standardized points (2 on the margins, 2 at 0.75mm above the margin, 2 on the axial walls and 1 on the occlusal surface), as follows (Figure 16-18): 87 1. Use Analysis>Dimensions>Section Through Object to create cross sections 2. Go to Analysis>Dimensions>2D Dimension to define the 7 points Figure 16. Grooves on the base of the Master Die in order to standardize the sections. 88 Figure 17. Facial-lingual section with the 7 standardized points. Figure 18. Mesial-distal section with the 7 standardized points. 89 The steps taken to obtain the measurements using the Geomagic Qualify 2012 software are the following: 1. Right Mouse Click on the Master Die to set as “Reference” and again on the crown to set it as “Test”. 2. The intaglio of the crown and the coronal part of the master die are selected 3. Go to Alignment>Object Alignment>Best-fit Alignment. Click Apply twice for fine adjustments. Click OK. 4. A macro program was created that automated the following steps. a. Reverse the normals: where the test object becomes the intaglio of the crown instead of the outside, which the software automatically selects. b. Reduce noise prismatic (conservative) twice c. Remove Spikes at 70% d. The intaglio is set as an independent object. e. AutoCreate features from the Reference Object over to the Test Object. In order to Autocreate the features only one crown was used and then the point targets were applied to the rest of them. The following steps describe the point target positioning: i. Select the inside of the crown. ii. Right Mouse Click>Reverse Selection. iii. Tools>Create>New Object>From Selection iv. Select the inside of the crown. v. Polygons>Repair>Repair>Flip Normals vi. Select the tooth. 90 vii. Alignment>Object Alignment>Best Fit alignment viii. Analysis>Compare>3D Compare a. Set the Objects box to Compare Selected Only b. Set the Max Deviation to .75mm c. Set the Max Critical and Max Nominal to .06mm d. Set the Min Critical and Min Nominal to .06mm ix. This creates a color map of highs and lows of the alignment. Use Create Annotations to annotate the low spots (blue). x. Export the table of the XYZ positions of the Annotations xi. Use the XYZ coordinates from the annotations to create the Point Target features 5. Go to Analysis >Section Through Object to section the tooth through the grooves to evaluate the fit. If there was an overlap, where the crown seemed to be inside the tooth, the crown needed to be repositioned. 6. Go to Tools >Move >Exact movement. The crown was moved in any direction and as many times as needed, until the most realistic and acceptable seating was established. 7. An “Automation” was created that included the following steps: a. RPS Alignment 91 b. 3D Comparison around the margin, as well as the c. 2D measurements on the pre-determined locations. 8. All the data were obtained. For the 3D data go to “Export table” and save the values in an Excel file. For the 2D data, go to “Create report” and save it as a PowerPoint file. Statistical analysis Two different data sets were created, one for the 3D and one for the 2D data obtained from the measurements. For the 3D data, descriptive statistics were conducted. One-way ANOVA with post-hoc Tukey’s HSD (Honestly Significant Difference) test was used to determine whether there were significant differences in mean marginal gap values among the four experimental groups. Additionally, a two-way ANOVA was performed to detect a significant interaction between the type of impressions and the type of crowns on the marginal fit. For the 2D data, a two-way ANOVA was performed to detect a significant interaction between the type of impressions and the type of crowns on the marginal and internal fit. Two-sample t-test was used to detect the difference in marginal and internal fit between two types of crowns within each type of impressions or between two types of impressions within each type of crowns. Additionally, when the test groups were considered as four individual experimental groups, one-way ANOVA with post-hoc Tukey’s HSD (Honestly Significant Difference) test was used to determine whether there 92 was a significant difference in the mean marginal and internal gap values among the four experimental groups. A p-value of less than 0.05 was used as a criterion for statistical significance. Statistical analyses were carried out with the statistical package SAS® System version 9.3 (SAS Institute Inc, Cary, NC, USA). Note: (i) An interaction is a condition in which the effect of one independent variable on the dependent variable is different at different levels of the second independent variable. In this study, independent variables were crown and impression, while dependent variable was marginal fit. For example, a significant interaction between impression and crown on marginal fit means that the influence of the type of crowns on marginal fit was different for the two types of impression or the influence of the type of impressions on marginal fit was different for the two types of crown. If an interaction is non-significant, only the main effects are tested, whereas when a significant interaction exists, the simple effects must be tested. (ii) If there is a significant simple effect for independent variable A at given level of independent variable B, it means that there is a significant relationship between independent variable A and the dependent variable at that level of independent variable B. 93 CHAPTER IV RESULTS Separate statistical analyses were conducted in order to assess the threedimensional (3D) and two-dimensional (2D) marginal and internal gaps of pressed and CAD lithium disilicate crowns made from conventional and digital impressions. 3D Results One-way ANOVA for 3D Marginal fit Sixty study samples were used (n=15/per group). Marginal gap value of each sample was an average of all 3D measurement values, and it was used for the statistical analysis. Negative values were excluded from the study (Figure 19). Figure 19. 3D measurement of the predefined area. 94 Table 6 and Figure 20 provide the final descriptive statistics of the 3D marginal gap values for each experimental group. Results of one-way ANOVA revealed that there was a significant effect for the type of the combinations of impression and crown on the 3D marginal fit (F(3,56)=15.73; p<0.0001). The post-hoc Tukey’s HSD test indicated that the mean 3D marginal gap for conventional impressions with pressed crown was significantly smaller than those obtained from other three experimental groups, and no significant differences were found among digital impressions with pressed crown, conventional impressions with CAD crown, and digital impressions with CAD crown. Table 6 provides detailed results from the post-hoc Tukey’s HSD test. Experimental Groups N 15 Mean Marginal Gap in mm (SD) 0.048 (0.009) Group Comparisons** B PVS/press (Group A) PVS/CAD (Group B) Lava/press (Group C) Lava/CAD (Group D) 15 15 15 0.088 (0.024) 0.089 (0.020) 0.084 (0.021) A A A Table 6. Mean 3D marginal gap in mm of 4 experimental groups. ** means with the same letter are not significantly different using the post-hoc Tukey’s HSD test (P>.05). 95 0.12 0.1 0.08 0.06 mm 0.04 0.02 0 PVS/press (Group A) PVS/CAD (Group B) Lava/press (Group C) Lava/CAD (Group D) Figure 20. Mean 3D marginal gap of 4 experimental groups. Two-way ANOVA for 3D Marginal fit Results of two-way ANOVA revealed that there was a significant interaction between impression techniques and crown types (F(1, 56)=20.10; p<0.0001). Subsequent analyses demonstrated that there was a significant simple effect for the type of crowns with conventional impression (p<0.0001) using a two-sample t-test. Results showed that the mean 3D marginal gap for the pressed crowns was significantly smaller than that for the CAD crowns (0.0476mm vs. 0.0877mm). However, no significant simple effect was found for the type of crowns with digital impression. That is, with digital impressions, no significant difference was found in the mean 3D marginal gap between the two crown types (p=0.5574). 96 Analysis also showed that there was a significant simple effect for the type of impressions with pressed crown (p<0.0001) using a two-sample t-test. Results showed that the mean 3D marginal gap for the conventional impressions was significant less than that for the digital impressions (0.0476mm vs. 0.0887mm). However, no significant simple effect was found for the type of impressions with the CAD crowns. That is, with the CAD crowns, there was no significant difference between the two impression techniques (p=0.672). 2D Results One-way ANOVA for 2D Marginal fit Results of one-way ANOVA revealed that there was a significant effect for the type of experimental groups on the 2D marginal fit (F(3,56)=12.27; p<0.0001). Detailed results of one-way ANOVA are shown in Table 7. Subsequently, the post-hoc Tukey’s HSD test indicated that the 2D marginal gap obtained from the PVS-press group was significantly smaller than other three groups, while no significant difference was found among those three groups. Source d.f. SS MS F Type of experimental groups 3 0.0139 0.0046 12.27 Within groups 56 0.0211 0.0004 Total 59 0.0350 d.f.: degrees of freedom; SS: sum of squares; MS: mean square; N=60 Table 7. Result of One-Way ANOVA for 2D marginal fit. P value <0.0001 97 Two-way ANOVA for 2D Marginal fit Results of two-way ANOVA revealed that there was a significant interaction between impression and crown on the 2D marginal fit (F(1, 56)=14.32; p=0.0004). Detailed results of two-way ANOVA are shown in Table 8. Source d.f. SS MS F P value Type of crowns 1 0.0044 0.0044 11.74 0.0012 Type of impressions 1 0.0041 0.0041 10.76 0.0018 Interaction between type of crowns and type of impressions 1 0.0054 0.0054 14.32 0.0004 Within groups 56 0.0211 0.0004 Total 59 0.0350 d.f.: degrees of freedom; SS: sum of squares; MS: mean square; N=60 Table 8. Result of Two-Way ANOVA for 2D marginal fit. Since a significant interaction was found, further analyses for simple effects were conducted. The subsequent analyses demonstrated that there was a significant simple effect for the type of crowns with PVS impression on the 2D marginal fit (p<0.0001, twosample t-test). Results showed that the mean 2D marginal gap for the pressed crowns was significantly smaller than that for the CAD crowns (0.0399mm vs. 0.0760mm). However, no significant simple effect was found for the type of crowns with the digital Lava impressions (p=0.8177, two-sample test). That is, with the Lava digital impressions, no 98 significant difference was found in the 2D marginal fit between the two crown types (Table 9). Moreover, the data also showed that there was a significant simple effect for the type of impressions with the pressed crowns on the 2D marginal fit (p<0.0001, twosample t-test). Results showed that the mean marginal gap for the PVS impressions was significantly less than that for the digital impressions (0.0399mm vs. 0.0753mm). However, no significant simple effect was found for the type of impressions with the CAD crowns (p=0.7812, two-sample t-test). That is, with the CAD crowns, there was no significant difference between the two impression techniques (Table 9). Table 9 and Figure 21 provide the mean marginal gap and standard deviation of the 2D marginal gap values for each experimental group. Experimental groups N PVS/press (Group A) PVS/CAD (Group B) Lava/press (Group C) Lava/CAD (Group D) 15 15 15 15 Mean Marginal Gap in mm Group (SD) Comparisons** 0.0399 (0.0086) B 0.0760 (0.0234) A 0.0753 (0.0148) A 0.0735 (0.0258) A Table 9. Pairwise comparisons of mean 2D marginal gap by experimental group. ***means with the same letter are not significantly different using post-hoc Tukey’s HSD test (P>.05). 99 0.12 0.1 0.08 0.06 mm 0.04 0.02 0 PVS/press (Group A) PVS/CAD (Group B) Lava/press (Group C) Lava/CAD (Group D) Figure 21. Mean 2D marginal gap in mm. One-way ANOVA for 2D Internal fit Results of one-way ANOVA revealed that there was a significant effect for the crown fabrication techniques on the 2D internal fit (F(3,56)=25.91; p<0.0001). Detailed results of one-way ANOVA are shown in Table 10. Subsequently, the post-hoc Tukey’s HSD test indicated that the 2D internal gap obtained from the Lava/press group was significantly greater than other three groups, while no significant differences were found between Lava/CAD and PVS/CAD, and between PVS/press and PVS/CAD. Table 10 provides detailed results from the post-hoc Tukey’s HSD test. 100 Source d.f. SS MS Type of experimental groups 3 Within groups 56 0.0690 0.0012 Total 59 0.1647 F P value 0.0957 0.0319 25.91 <0.0001 d.f.: degrees of freedom; SS: sum of squares; MS: mean square; N=60 Table 10. Result of One-Way ANOVA for 2D internal fit. Two-way ANOVA for 2D Internal fit Results of two-way ANOVA revealed that there was a significant interaction between impression technique and crown type on the 2D internal fit (F(1, 56)=15.22; p=0.0003). Detailed results of two-way ANOVA are shown in Table 11. Source d.f. SS MS F P value Type of crowns 1 0.0136 0.0136 11.03 0.0016 Type of impressions 1 0.0634 0.0634 51.47 <0.0001 Interaction between type of crowns and type of impressions 1 0.0187 0.0187 15.22 0.0003 Within groups 56 0.0690 0.0012 Total 59 0.1647 d.f.: degrees of freedom; SS: sum of squares; MS: mean square; N=60 Table 11. Result of Two-Way ANOVA for 2D internal fit. 101 Since a significant interaction was found, further analyses for simple effects were conducted. Subsequent analyses demonstrated that there was no significant simple effect for the type of crowns with the PVS impressions on the 2D internal fit (p=0.6967, twosample t-test). That is, with PVS impression, no significant difference was found between the two types of crown fabrication methods. However, results showed that there was a significant simple effect on the 2D internal fit for the type of crowns with the digital impressions (p<0.0001, two-sample t-test). The mean 2D internal gap for the pressed crowns was significantly greater than that for the CAD crowns (0.2109mm vs. 0.1454mm) (Table 12). Moreover, data also showed that there was a significant simple effect for the type of impressions with the pressed crowns on the 2D internal fit (p<0.0001, two-sample ttest). Results showed that the mean 2D internal gap for the PVS impressions was significantly smaller than that for the digital impressions (0.1105mm vs. 0.2109mm). Also, a significant simple effect was found for the type of impressions with the CAD crowns on the 2D internal fit (p=0.0011, two-sample t-test). That is, with the CAD crowns, the mean 2D internal gap for the PVS impressions was significantly smaller than that for the digital impressions (0.1158mm vs. 0.1454mm) (Table 12). Table 12 and Figure 22 provide the final descriptive statistics of the 2D internal gap values for each experimental group. 102 Experimental groups N 15 Mean Internal Gap in mm (SD) 0.1105 (0.0474) Group Comparisons** C PVS/press (Group A) PVS/CAD (Group B) 15 0.1158 (0.0200) B, C Lava/press (Group C) 15 0.2109 (0.0410) A Lava/CAD (Group D) 15 0.1454 (0.0245) B Table 12. Pairwise comparisons of mean 2D internal gap by experimental group. ***means with the same letter are not significantly different using post-hoc Tukey’s HSD test (P>.05). 0.3 0.25 0.2 0.15 mm 0.1 0.05 0 PVS/press (Group A) PVS/CAD (Group B) Figure 22. Mean 2D internal gap in mm. Lava/press (Group C) Lava/CAD (Group D) 103 CHAPTER V DISCUSSION One of the main purposes of the study was to evaluate if there was an interaction between the impression technique (conventional and digital) and the crown fabrication method (press and CAD/CAM), considering the marginal and internal fit of the crowns fabricated by any combination of those methods. There were no studies in the literature to assess the adaptation of all-ceramic crowns made from the combination of those techniques. Therefore, this study provided information as to how either impression technique can be combined with either crown fabrication method in order to facilitate the needs or the availability of each clinical practice. The marginal fit was the main parameter tested, as it has been used extensively in the literature for similar studies, since it is very critical for the quality and success of the prostheses. Moreover, the internal fit was assessed in order to obtain a more complete picture as to how the crowns actually adapted to the tooth surface. This study, not only tested the new technology available in dentistry, but also utilized similar industrial methodology in obtaining the measurements of the digital specimens. The 3D and 2D data were obtained by digitizing the physical specimens. The digital models could be moved, rotated, sectioned and measured as many times and in any way as desired. This method has the potential of providing a vast amount of information that was not possible before. Results from this study indicated that the combination of conventional (PVS) impression method and press fabrication technique produced the most accurate 3D 104 (0.048mm) and 2D (0.04mm) marginal adaptation. These results coincide with the findings of previously published studies that compare the fit of pressed crowns to that of cast crowns (which is still considered the gold standard) (72, 73). More specifically, Baig et al. compared the marginal fit with respect to gap and overhang of zirconia (Cercon Y-TZP) ceramic crowns to lithium disilicate pressable (IPS Empress II) and complete metal crowns (control group) using a computerized digital image analysis system (72). They found that the mean marginal gap for those three groups was 66.4µm, 36.6µm and 37.1µm, respectively. The significantly higher marginal gap of the Cercon crowns compared to IPS Empress II crowns was attributed to the type of manufacturing of these 2 ceramic systems; the distortion of the ceramic coping was thought to be less in IPS Empress II compared with Cercon, as the pressed technique involves a less complicated process, using a more manual than computer program computation, and is process dependent. Similarly, Stappert et al. evaluated the marginal accuracy of partial coverage restorations made from gold, hybrid composite (Targis), IPS e.max Press, IPS-Empress and ProCAD/Cerec under mouth-motion fatigue and thermal cycling (73). They found that after cementation, the IPS e.max Press had similar marginal fit to gold and IPS-Empress whereas the ProCAD group demonstrated decreased marginal fit. Both of the above studies compared the pressed IPS e.max Press crowns to CAD crowns. The marginal fit of the pressed crowns were 36.6µm and 60[52–67]µm respectively. Despite the difference in the measurement methods, the present study found very similar results regarding the marginal fit (3D: 48µm and 2D: 40µm) of the pressed crowns from the conventional impressions. 105 The surprizing finding, in the present study, was that the pressed crowns made from the digital impressions (Group C) had a statistically significant larger marginal gap than the pressed crowns made from the conventional impressions (Group A). This means that the combined techniques (digital impression/pressed crowns) were not as accurate as the conventional impression/pressed crown combination. However, no difference was found between the CAD/CAM crowns made from the conventional (Group B) and the digital impressions (Group D), indicating that the combined techniques had similar accuracy. Direct comparison between the pressed and the CAD/CAM fabrication techniques should be done with caution, since the production methods are so different; in other words, since for the CAD/CAM crowns the dies were scanned, there was the possibility that any irregularities on the dies were “smoothed out” by the software in order to facilitate the crown fabrication. The reason why the marginal gap of the Group B was statistically larger than that of Group A, might be attributed to the overall fit of the crown, which may have been affected by the internal adaptation. A thorough explanation regarding the internal fit of Group C will follow below where the internal fit of the crowns is discussed. The early CAD/CAM systems were developed with the goal to simplify the processing of dental prostheses while producing accurately fitted restorations. In the 1980s, this was not the reality due to the increased total cost, the increased operation time and manipulation of the systems in comparison to the conventional techniques, inaccurate mechanical milling of sharp corners and delicate margins (111). In addition to that, the low resolution scanning and inadequate computing power also resulted in poor marginal and internal fit of the produced prostheses (71). However, advancements in technology, 106 engineering and chemistry since then has led to the great variety of CAD/CAM systems available today that use highly accurate scanners, more sophisticated software with high precision in digitizing the complex shapes required in dentistry (70, 111, 112). In this study, two of the newly introduced CAD/CAM systems were used; the Lava™ C.O.S. (testing the impression technique) and the E4D scanner and milling engine (testing the crown fabrication method). Taking into consideration the results of either the 3D or the 2D measurements, no statistically significant differences were found among the remaining three experimental groups; Conventional Impression/CAD Crown (Group B), Digital Impressions/Pressed Crown (Group C), and Digital Impression/CAD Crown (Group D). This means that, if we compare within the digital impression groups (C and D), the fabrication of either a press or a CAD lithium disilicate crown will result to similar marginal fit. Also, those results show that CAD/CAM crown margins can be accurately produced when either a stone or a SLA model are scanned using the E4D scanner. Despite the statistical difference between Group A and the remaining three groups for the marginal fit, the clinical difference might not be of great importance. Specifically, the marginal gaps for Groups B, C, and D in 3D were 0.088mm, 0.089mm and 0.084mm and in 2D were 0.076mm, 0.075mm and 0.074mm, respectively. It has been established that a gap of less than 120µm is the clinical acceptable limit (8, 95). Research is ongoing on digital impressions and several studies have been conducted to evaluate the fit of the newly introduced systems. Similar to this study, only in vivo, Syrek et al. compared the fit of all-ceramic crowns fabricated from intraoral digital impressions with the fit of all-ceramic crowns fabricated from PVS impressions 107 (58). Two LavaTM (zirconia) crowns were fabricated for the same preparation in each of 20 patients. One crown was fabricated using the Lava™ C.O.S. and the other crown from the PVS impression material. Prior to cementation, two calibrated and blinded examiners clinically evaluated the fit of both crowns. The marginal fit was also scored using the replica technique. The results revealed significant difference between the two groups; allceramic crowns resulting from Lava™ C.O.S. demonstrated significantly better marginal fit than all-ceramic crowns fabricated from conventional impressions (both groups were within clinically acceptability). Scotti et al. published a very similar paper to the above, where they measured the marginal and internal fit of zirconia crowns made from Lava™ C.O.S. using the replica technique (55). Four different points were evaluated using stereomicroscopy with a magnification of 50x. The results showed that all values were clinically acceptable and the authors considered this method to be accurate enough to be used as an alternative to conventional impressions. Both studies found that all-ceramic CAD/CAM crowns (zirconia) made from the Lava™ C.O.S. were as accurate or even more than the ones made from PVS impressions. Similar findings were shown in the present study; when the CAD crowns (IPS e.max CAD), made from conventional and Lava™ C.O.S. impressions (Group B vs. D), were compared, no significant differences were found for both 3D and 2D measurements. With regard to the internal fit, when the crown fabrication method was compared within the digital impression groups (C and D), Group C (Lava/press) appeared to have significantly bigger internal gap than Group D (Lava/CAD) (0.2109mm vs. 0.1454mm). The same result was noted when the two impression techniques were compared within the pressed groups (Group A and C). In that case Group C had significantly bigger 108 internal gap than Group A (PVS/press) (0.2109mm vs. 0.1105mm). Furthermore, when all groups were considered as four individual experimental groups, and one-way ANOVA with post-hoc Tukey’s HSD test was used to determine whether there was a significant difference in the mean internal gap values among the groups, the only group with statistical difference in internal fit was Group C (Lava/press). This showed that consistently with any combination of analyses that when the pressed crowns were fabricated on the SLA dies they had a larger internal gap. The 2D digital sections provide the ability to see the adaptation of the crown on the die surfaces, and by evaluating those, it was found that two of the crowns had very poor internal adaptation. The data of those two were removed and the statistical analysis was repeated, thinking that maybe the difference was due to those two “outliers”. However, the new analysis showed the same statistical significance as the first one, indicating that the Group C had poorer internal adaptation (a larger gap) when compared to the remaining three groups. A suggestion for this occurrence was thought to be possible irregularities on the SLA model surfaces as a result of the fabrication procedure that would not allow a uniform internal adaptation of the crowns. The two SLA dies that produced the most irregularly fitted pressed crowns were subsequently examined under the microscope (10x), and no defects were detected. If there were defects on the SLA dies, since they were randomly divided, the same appearance should be detected for Group D (Lava/CAD) that also had the same type of SLA models. As mentioned above, the fact that this was not noticed in that group might suggest that surface irregularities were not the reason for poor adaptation, but it cannot be concluded because of the difference in crown fabrication technique between the two groups. For the CAD/CAM crowns, the 109 SLA models were scanned, which could change the model surface either because of the resolution of the scanner or because of processing of the software that would eliminate any defects interfering with the CAD/CAM procedure. Another suggestion for the poorer internal fit of Group C (Lava/press) was the use and the type of die spacer. The die spacer used for this study was rubber-based, recommended by the manufacturer (Ivoclar Vivadent). Although, this type of material was used successfully with the stone dies, it might not be compatible with the SLA resin models, leading to poor wettability of the dies. This would result in the material not adapting evenly on all surfaces of the die and would cause a non-uniform or irregular appearance of the intaglio of the crowns, as seen in Group C. This assumption is further supported by the fact that, there was no statistical difference in the marginal fit of this group when compared to B and D; since the area where the marginal gap measurements were taken was not covered by die spacer. For the internal fit, the results also revealed that within the CAD/CAM crown fabrication method groups (Group B and D), the mean internal gap for PVS impression (Group B) was significantly smaller than that for digital impression (Group D) (0.1158mm vs. 0.1454mm). In other words, the combination of digital impression and CAD/CAM crowns produced a poorer internal adaptation compared to the conventional impression and CAD/CAM crown. The E4D scanner has established protocols for 3 different types of scanning: a. Intraoral scanning or direct digitalization, b. Scanning of the impression or indirect digitalization and c. Scanning of the cast/model or indirect digitalization. This scanner, therefore, should be able to capture data from surfaces with different properties including translucency, reflection, smoothness etc. For this project, 110 the indirect digitalization was used, by scanning the stone and SLA models. The gypsum and the resin dies have very different surfaces with stone being more opaque and smooth and the resin being more reflective and less smooth due to the lines produced when printed. It may be assumed that the scanner did not capture the data from the SLA model with as high a degree of accuracy. It is common that most of the scanners available today, in order to accurately capture the surface, need it to be coated with an opaque spray or dye. The E4D claims to achieve that accuracy without the use of a spray, but it has not been tested with SLA models before. Hirayama et al. evaluated the internal and marginal fit of zirconia copings generated from scanning definitive casts made from a conventional impression and copings generated from a digital impression system (125). Specimens were divided into 2 groups: group A, Zirconia copings fabricated by scanning stone models from PVS impressions and group B, Zirconia copings fabricated by scanning the master cast and transmitting the scanned data to the Lava™ milling center in St. Paul, MN. To evaluate the fit, the authors used the replica technique described by Boening et al. (32) and Molin and Karlsson (31). They found that group B had a statistically better marginal adaptation than group A. That study concluded that the both groups of copings had clinically acceptable marginal fit; however the ones made form the digital impression had significantly smaller marginal discrepancies. In this study, when the CAD/CAM crowns (IPS e.max CAD) (Group B vs. D) were compared the crowns made from the Lava™ C.O.S. did not appear to have a statistically better marginal or internal fit than the ones made from the conventional impressions. However, both groups had clinically acceptable adaptation. 111 As described previously, Tuntiprawon and Wilson evaluated the effect of increasing cement thickness (using platinum foil and die spacer) on the fracture strength of all-ceramic crowns (14). They found that there was a decrease in strength when the cement thickness was increased. The strength decrease was possibly attributed to the greater deformation of the ceramic into the cement as well as the decreased thickness of the crown itself. In this study the internal gap for Group C (Lava/press) at 0.2109mm was almost twice as much as Group A (PVS/press) 0.1105, and significantly bigger than the remaining two groups (0.1158mm and 0.1454mm respectively). This may result in reduced fracture strength due to greater deformation of the ceramic into the cement when the all-ceramic crowns are made with this combination of impression/crown fabrication techniques, however this was not tested in this study. Study design As mentioned previously, an important limitation of the CAD/CAM prostheses is marginal chipping due to machining induced damaged. Tsitrou et al. conducted a study in order to investigate any possible correlation between the brittleness index (BI) of machinable dental materials and the chipping factor (CF) of the final restorations (113). The materials tested were Paradigm MZ100TM (3M/ESPE), Vita Mark II (VITA), ProCAD (Ivoclar-Vivadent) and IPS e.max CAD (Ivoclar-Vivadent) milled in CEREC system (Sirona Dental Systems Gmbh, Bensheim, Germany). Tsitrou et al. showed that the CF increases as the BI increases, with Paradigm MZ100 having the lowest BI and CF, and IPS e.max CAD demonstrating the highest BI and CF. Giannetopoulos et al. 112 investigated whether the CAD/CAM system would influence the marginal integrity of IPS e.max CAD copings made with different finishing lines (114). The Cerec inEOS system/Cerec 3D software and KAVO Everest system were compared. The finish lines were 60-degree bevel, 30-degree bevel and 0-degree bevel (or 90-degree shoulder). The CF was calculated, as in the above study (113); the CF was increased as the bevel angle increased for Cerec group, whereas this factor did not influence the marginal integrity for the Everest group. The conclusions of the study were that different milling processes produce different amount of marginal chipping, although that was apparent only for the 60-degree bevel. Taking into consideration those two studies and the potential of marginal chipping, in this study, the IPS e.max CAD crowns were designed with an overhang. This limited the machining induced damage that could affect the results. After the crowns were milled and before they were crystallized, they were adjusted under microscope using a diamond-polishing disk, as recommended by the manufacturer (Ivoclar Vivadent). Any chipping from the adjusting would be expected to be to a lesser degree compared to the machining and it simulates normal clinical conditions. However, no chipping was observed during the adjustment under the microscope. Multiple techniques have been described in literature in order to measure the marginal and internal fit of full coverage restorations. The most commonly found nondestructive technique in literature is the replica technique (31-37). Replicas of the intermediate space between the inner surface of the crown and tooth surface are made by the method that was described by Molin and Karlsson (31). The important characteristic of this technique is that it can be used in vivo as well as in vitro since it does not involve the destruction of the specimens. However, handling of the materials, applied pressure 113 during setting, and positioning under the microscope are some of the limitations of this technique. For this particular study, a newly introduced digital method was selected for data acquisition using the new triple-scan protocol developed by Holst et al. (97). By using a non-contact scanner, three scans were performed: 1) Coping solo, 2) Master cast solo and 3) Coping placed on master cast in a clinically correct final position. After the objects (coping and master cast) were digitized, STL files were generated from point clouds with the scanner software (ATOS system, GOM mbH, Braunschweig, Germany). First, the master cast STL file and the coping-master cast STL file were registered by manual alignment followed by best-fit registration. Then the same was done to match the coping and coping-master cast. The coping-master cast data was deleted and the aligned coping to master cast data were used for fit assessment. To measure the cement gap, the outer surface of the coping was deleted, followed by reversion of the surface normal of the intaglio of the coping and calculation of deviations from the master cast. The authors recommended that where high accuracy measurements are needed, a high precision coordinate measurement machine (CMM) integrated with 3D laser scanning system should be used (97). In addition, the digital measurement technique was validated before applying it to the definitive study. Part of the pilot study was the comparison of the new technique with the replica technique that is well documented in the literature (31, 32, 96). Seven out of ten variables had no statistical difference indicating that the two techniques produced similar results. The variables that were significantly different were mainly the occlusal measurements, which generally had a greater gap and variability (based on SD). No 114 difference was found in the margins, which was considered the more critical area of measurement. In the pilot study, the same scanner was used for both the fabrication of the CAD crowns and the digitizing of the specimens. In order to avoid errors that could be introduced by using the same scanner, for the final study, a CMM, the Surveyor ZSSeries scanner with scan accuracy of +/- 0.00898mm (Laser Design Inc, GKS, Minneapolis, MN, USA), was used. The selection for the particular type of scanner was based on Holst’s et al. recommendation that a CMM system should be used in order to assure the best capture of the surfaces tested (98). This assured that any problems with the scanner produced CAD crowns were not transferred to the next step of the study. Moreover, by using such an accurate scanner that is used in several fields of engineering with complex shapes and targets it was the best option available for the accuracy of this project. Usually for highly reflective or translucent materials (such as all-ceramic crowns) the surfaces need to be coated which may introduce error. Similarly, in this case, although low translucency ingots were used, the use of spray was still necessary to acquire the best data quality, according to the operators of the scanner. After the specimens were digitalized any type of section and measurement was possible. Most of the studies, until now, presented 2D data, with fiduciary marks, either sectioned or not, taken by photos or with microscopes (31-37). This limits the results to the specific point measured without providing with any information for the fit as a whole. The replica technique produced a 3D representation of the cement space but the actual measurements were taken from the sections of it. A 3D measurement was not possible 115 with any of those instruments or techniques used. Holst et al. developed the triple scanning protocol and made it possible to obtain the 3D fit of the entire contacting surface (97). For the 3D measurement, all the values (25,000-30,000) obtained by Geomagic software were averaged and the mean was used for the comparisons. Any negative values (denoting that the crown was smaller than the tooth) were excluded from the calculations, since they do not represent a realistic scenario, but only a limitation of the software. In this study, two different types of measurements were made by utilizing the capacities of the software. The 2D measurements were done by sectioning the digital specimens and defining 7 fiduciary points in each section. With those sections, it was easier to visualize the internal gap in each part of the tooth desired, and also to compare it with previous studies in the literature. However, it should be taken into account, that direct comparisons with previous studies reporting 2D measurements made from physical sections should be avoided because of the innate difference of a physical to a digital model. For the statistical analysis, all the measurements obtained from each of the 7 fiduciary marks, where averaged, and the internal fit was one variable, instead of having 14 variables for each crown, that would complicate and obscure the analysis. The marginal fit, as previously described, is considered the most important criterion for clinical success and longevity, was assessed both by 3D and 2D measurements. The 2D data set was the continuum of the internal fit fiduciary marks that were defined, visually showing the distance of the intaglio from the tooth. The 3D measurement provided a qualitative colored map of the defined area, indicating the adaptation circumferentially, showing what other studies had failed to show before. The 116 width of the measured area (0.75mm) was identified as the area below the die spacer, where the closest proximity is expected. The fact that the 2D and 3D measurements resulted in the same conclusion, that the Group A had the smallest marginal gap, with no difference among the remaining three groups, validated the reliability of the software and the protocol used. The consistent 10µm difference between the two data sets was due to the different processing of the software when calculating 3D versus 2D data, and not due to an error in the developed protocol. Possible limitations of the study The main limitation of the study was the software used for the obtaining the measurements. After using the Best-Fit Alignment twice, the fit had to be adjusted manually in order to simulate a clinically relevant crown position. The Best-Fit Alignment finds the path of least resistance between the two objects and then superimposes them. This may result in a situation where the crown seems to be “inside” the tooth. Each time a crown was fitted, a section was made and if it seemed like it is “inside” the tooth, it was manually moved until the closest marginal and internal fit was achieved. However, the fact that the fit was improved at the given section does not mean that it was correctly seated three dimensionally. The protocol used for the digital fitting was developed with the assistance of the Geomagic technical support and this was the best result they could assure. Other limitations include the use of rubber-based die spacer on the SLA models, the grinding of the overhanging margin of the CAD crowns and the spray used for 117 digitizing the crowns and the master die. In addition, the crowns were not cemented on the master die as they would in a clinical setting; cementation may produce different results. Avenues for future research With the introduction of the 3D digital measurements, by using the triple scanning protocol by Holst at al. (97) and a sophisticated processing software, the door has opened to unlimited research in the fit of dental restorations. The standardization, the nondestructive nature, the rapid processing of data and the storage/availability of that information will enable the comparison and analysis of any material desired. The infinite designs of measurements will allow for very detailed and consistent data acquisition. Clinical significance Any combination of PVS or Lava™ C.O.S. impression with press or CAD lithium disilicate crowns will result to a clinically acceptable restoration considering the marginal and internal fit. However, when the strength of the crown is taken into consideration, the increased internal gap of the all-ceramic pressed crowns made from Lava™ C.O.S. impressions and SLA dies may predispose to a reduced fracture resistance that could compromise the longevity and survival of the restoration. 118 CHAPTER VI CONCLUSIONS This study examined the three-dimensional and two-dimensional marginal and internal fit of pressed and CAD/CAM lithium disilicate crowns made from conventional and digital impressions. Based on the results the null hypotheses were rejected and the alternative were accepted: • There was a difference in accuracy between the two impression techniques, conventional and digital, considering the marginal and internal fit of the crowns fabricated by those methods. • There was a difference in accuracy between the crown fabrication methods, press and CAD/CAM, considering the marginal and internal fit of the crowns fabricated by those methods. • There was an interaction between the impression technique (conventional and digital) and the crown fabrication method (press and CAD/CAM), considering the marginal and internal fit of the crowns fabricated by any combination of those methods. Within the limitations of the present study, the following conclusions were drawn: • The combination of the conventional impression and the pressed crown produced the most accurate marginal fit. 119 • There was no statistical difference among the conventional impression/CAD crown, digital impression/ press crown and digital impression/CAD/CAM crown with regard to the marginal fit. • The combination of the digital impression and pressed crown produced the least accurate internal fit. • There was no statistical difference among the conventional impression/CAD crown, conventional impression/press crown and digital impression/CAD crown with regard to the internal fit. • All combinations produced crowns with clinically acceptable marginal fit. 120 APPENDIX A RAW DATA Table A1. Average marginal gap for 3D measurements. SAMPLE 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1 2 3 4 GROUP 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 MARGINAL GAP 0.0593 0.0378 0.0595 0.0494 0.0452 0.0427 0.0389 0.0559 0.0441 0.0542 0.0581 0.0403 0.0548 0.0395 0.0336 0.0791 0.0922 0.1278 0.0923 0.132 0.1038 0.0695 0.0797 0.0561 0.0691 0.0715 0.0613 0.1191 0.0744 0.0877 0.0826 0.0703 0.1116 0.0889 CROWN 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 1 1 IMPRESSION 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 121 5 6 7 8 9 10 11 12 13 14 15 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 Table A1. Continued. 0.0809 0.0498 0.1134 0.0805 0.0915 0.0843 0.0834 0.0969 0.0814 0.1346 0.0798 0.0534 0.1184 0.0848 0.0689 0.0684 0.0803 0.0623 0.1185 0.0771 0.0885 0.068 0.0845 0.1245 0.0918 0.0734 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 122 Table A2. Average marginal and internal gaps for 2D measurements. SAMPLE 1 2 3 4 5 6 7 8 19 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 GROUP 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 MARG. GAP 0.047 0.036125 0.0485 0.04475 0.028125 0.03575 0.04175 0.04525 0.04275 0.059625 0.037375 0.04 0.033 0.027875 0.030375 0.075125 0.08375 0.11175 0.0835 0.101875 0.092375 0.064375 0.068875 0.040875 0.045875 0.057375 0.049125 0.116375 0.061875 0.0875 0.08675 0.062625 0.08975 0.067625 0.061125 0.0445 INTER. GAP 0.08 0.056916667 0.124666667 0.103 0.082333333 0.064166667 0.099333333 0.132166667 0.2535 0.085333333 0.112666667 0.138333333 0.142 0.104833333 0.0785 0.124 0.126 0.139333333 0.0855 0.134 0.134 0.117666667 0.095166667 0.105166667 0.091 0.115666667 0.103333333 0.157166667 0.102333333 0.106333333 0.222166667 0.175166667 0.262166667 0.259666667 0.157333333 0.164333333 IMPRESSION 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 CROWN 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 1 1 1 1 123 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 2 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 Table A2. Continued. 0.087875 0.0775 0.089375 0.06775 0.077125 0.09075 0.0545 0.08925 0.083125 0.037875 0.119125 0.070875 0.0455 0.068125 0.075875 0.048875 0.107625 0.0655 0.070125 0.04775 0.076375 0.116625 0.095875 0.056625 0.243333333 0.194833333 0.216 0.187333333 0.177 0.2305 0.201 0.176 0.296333333 0.114833333 0.1575 0.164 0.116666667 0.1335 0.112 0.107666667 0.167333333 0.149833333 0.173166667 0.131833333 0.163666667 0.184833333 0.153833333 0.150833333 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 124 APPENDIX B GEOMAGIC IMAGES Three-dimensional (3D) and two-dimensional (2D) images of one indicative specimen from each experimental group. Group A Figure B1. Group A (PVS/press) 3D colored map. 125 Figure B2. Group A (PVS/press) Facial-Lingual section for 2D measurements. Figure B3. Group A (PVS/press) Mesial-Distal section for 2D measurements. 126 Group B Figure B4. Group B (PVS/CAD) 3D colored map. 127 Figure B5. Group B (PVS/CAD) Facial-Lingual section for 2D measurements. Figure B6. Group B (PVS/CAD) Mesial-Distal section for 2D measurements. 128 Group C Figure B7. Group C (Lava/press) 3D colored map. 129 Figure B8. Group C (Lava/press) Facial-Lingual section for 2D measurements. Figure B9. Group C (Lava/press) Mesial-Distal section for 2D measurements 130 Group D Figure B10. Group D (Lava/CAD) 3D colored map. 131 Figure B11. Group D (Lava/CAD) Facial-Lingual section for 2D measurements. Figure B12. Group D (Lava/CAD) Mesial-Distal section for 2D measurements. 132 REFERENCES 1. White SN, Ingles S, Kipnis V. Influence of marginal opening on microleakage of cemented artificial crowns. J Prosthet Dent. 1994 Mar;71(3):257-64. 2. Schwartz NL, Whitsett LD, Berry TG, Stewart JL. Unserviceable crowns and fixed partial dentures: Life-span and causes for loss of serviceability. J Am Dent Assoc. 1970 Dec;81(6):1395-401. 3. Jahn KR, Baum W, Zuhrt R. Secondary caries frequency under complete crowns in relation to the material and design of the crown as well as the crown margin finish. Stomatol DDR. 1985 Nov;35(11):665-70. 4. Jacobs MS, Windeler AS. An investigation of dental luting cement solubility as a function of the marginal gap. J Prosthet Dent. 1991 Mar;65(3):436-42. 5. Bader JD, Rozier RG, McFall WT,Jr, Ramsey DL. Effect of crown margins on periodontal conditions in regularly attending patients. J Prosthet Dent. 1991 Jan;65(1):759. 6. Zoellner A, Bragger U, Fellmann V, Gaengler P. Correlation between clinical scoring of secondary caries at crown margins and histologically assessed extent of the lesions. Int J Prosthodont. 2000 Nov-Dec;13(6):453-9. 7. Reeves WG. Restorative margin placement and periodontal health. J Prosthet Dent. 1991 Dec;66(6):733-6. 8. McLean JW, von Fraunhofer JA. The estimation of cement film thickness by an in vivo technique. Br Dent J. 1971 Aug 3;131(3):107-11. 9. Campagni WV, Wright W, Martinoff JT. Effect of die spacer on the seating of complete cast gold crowns with grooves. J Prosthet Dent. 1986 Mar;55(3):324-8. 10. Eames WB, O'Neal SJ, Monteiro J, Miller C, Roan JD,Jr, Cohen KS. Techniques to improve the seating of castings. J Am Dent Assoc. 1978 Mar;96(3):432-7. 11. Grajower R, Lewinstein I. A mathematical treatise on the fit of crown castings. J Prosthet Dent. 1983 May;49(5):663-74. 12. Wilson PR. Effect of increasing cement space on cementation of artificial crowns. J Prosthet Dent. 1994 Jun;71(6):560-4. 13. Olivera AB, Saito T. The effect of die spacer on retention and fitting of complete cast crowns. J Prosthodont. 2006 Jul-Aug;15(4):243-9. 133 14. Tuntiprawon M, Wilson PR. The effect of cement thickness on the fracture strength of all-ceramic crowns. Aust Dent J. 1995 Feb;40(1):17-21. 15. Bottino MA, Valandro LF, Buso L, Ozcan M. The influence of cervical finish line, internal relief, and cement type on the cervical adaptation of metal crowns. Quintessence Int. 2007 Jul-Aug;38(7):e425-32. 16. Ayad MF. Effects of tooth preparation burs and luting cement types on the marginal fit of extracoronal restorations. J Prosthodont. 2009 Feb;18(2):145-51. 17. Fusayama T, Ide K, Hosoda H. Relief of resistance of cement of full cast crowns. J Prosthet Dent. 1964;14(1):95-106. 18. Holmes JR, Bayne SC, Holland GA, Sulik WD. Considerations in measurement of marginal fit. J Prosthet Dent. 1989 Oct;62(4):405-8. 19. Sorensen JA. A standardized method for determination of crown margin fidelity. J Prosthet Dent. 1990 Jul;64(1):18-24. 20. Hayashi M, Watts DC, Ebisu S, Wilson NH. Influence of vision on the evaluation of marginal discrepancies in restorations. Oper Dent. 2005 Sep-Oct;30(5):598-601. 21. Hayashi M, Wilson NH, Ebisu S, Watts DC. Influence of explorer tip diameter in identifying restoration margin discrepancies. J Dent. 2005 Sep;33(8):669-74. 22. Leknius C, Giusti L, Chambers D, Hong C. Effects of clinical experience and explorer type on judged crown margin acceptability. J Prosthodont. 2010 Feb;19(2):13843. 23. Assif D, Antopolski B, Helft M, Kaffe I. Comparison of methods of clinical evaluation of the marginal fit of complete cast gold crowns. J Prosthet Dent. 1985 Jul;54(1):20-4. 24. Brukl CE, Philp GK. The fit of molded all-ceramic, twin foil, and conventional ceramic crowns. J Prosthet Dent. 1987 Oct;58(4):408-13. 25. Hunt JL, Cruickshanks-Boyd DW, Davies EH. The marginal characteristics of collarless bonded porcelain crowns produced using a separating medium technique. Quintessence Dent Technol. 1978 Nov;2(9):21-6. 26. Romeo E, Iorio M, Storelli S, Camandona M, Abati S. Marginal adaptation of fullcoverage CAD/CAM restorations: In vitro study using a non-destructive method. Minerva Stomatol. 2009 Mar;58(3):61-72. 134 27. Tan PL, Gratton DG, Diaz-Arnold AM, Holmes DC. An in vitro comparison of vertical marginal gaps of CAD/CAM titanium and conventional cast restorations. J Prosthodont. 2008 Jul;17(5):378-83. 28. Gonzalo E, Suarez MJ, Serrano B, Lozano JF. Comparative analysis of two measurement methods for marginal fit in metal-ceramic and zirconia posterior FPDs. Int J Prosthodont. 2009 Jul-Aug;22(4):374-7. 29. Mitchell CA, Pintado MR, Douglas WH. Nondestructive, in vitro quantification of crown margins. J Prosthet Dent. 2001 Jun;85(6):575-84. 30. Pelekanos S, Koumanou M, Koutayas SO, Zinelis S, Eliades G. Micro-CT evaluation of the marginal fit of different in-ceram alumina copings. Eur J Esthet Dent. 2009 Autumn;4(3):278-92. 31. Molin M, Karlsson S. The fit of gold inlays and three ceramic inlay systems. A clinical and in vitro study. Acta Odontol Scand. 1993 Aug;51(4):201-6. 32. Boening KW, Wolf BH, Schmidt AE, Kastner K, Walter MH. Clinical fit of procera AllCeram crowns. J Prosthet Dent. 2000 Oct;84(4):419-24. 33. Coli P, Karlsson S. Fit of a new pressure-sintered zirconium dioxide coping. Int J Prosthodont. 2004 Jan-Feb;17(1):59-64. 34. Nakamura T, Tanaka H, Kinuta S, Akao T, Okamoto K, Wakabayashi K, et al. In vitro study on marginal and internal fit of CAD/CAM all-ceramic crowns. Dent Mater J. 2005 Sep;24(3):456-9. 35. Kokubo Y, Nagayama Y, Tsumita M, Ohkubo C, Fukushima S, Vult von Steyern P. Clinical marginal and internal gaps of in-ceram crowns fabricated using the GN-I system. J Oral Rehabil. 2005 Oct;32(10):753-8. 36. Kokubo Y, Ohkubo C, Tsumita M, Miyashita A, Vult von Steyern P, Fukushima S. Clinical marginal and internal gaps of procera AllCeram crowns. J Oral Rehabil. 2005 Jul;32(7):526-30. 37. Reich S, Uhlen S, Gozdowski S, Lohbauer U. Measurement of cement thickness under lithium disilicate crowns using an impression material technique. Clin Oral Investig. 2011 Aug;15(4):521-6. 38. Luthardt RG, Bornemann G, Lemelson S, Walter MH, Huls A. An innovative method for evaluation of the 3-D internal fit of CAD/CAM crowns fabricated after direct optical versus indirect laser scan digitizing. Int J Prosthodont. 2004 Nov-Dec;17(6):680-5. 135 39. Holst S., Tawdrous RE, Karl M. Description of a novel technique for threedimensional fit assessment of dental restorations. 6th world congress of biomechanics, IFMBE proceedings; August 1–6, 2010; Singapore. Springer; 2010. 40. Anusavice K, editor. Philips’ science of dental materials. 10th ed. ; 1996. 41. Powers JM SR, editor. Craig’s restorative dental materials. 12th ed. Elsevier; 2006. 42. Donovan TE, Chee WW. A review of contemporary impression materials and techniques. Dent Clin North Am. 2004 Apr;48(2):vi,vii, 445-70. 43. Michalakis KX, Bakopoulou A, Hirayama H, Garefis DP, Garefis PD. Pre- and postset hydrophilicity of elastomeric impression materials. J Prosthodont. 2007 JulAug;16(4):238-4. 44. Chee WW, Donovan TE. Fine detail reproduction of very high viscosity poly(vinyl siloxane) impression materials. Int J Prosthodont. 1989 Jul-Aug;2(4):368-70. 45. Johnson GH, Craig RG. Accuracy of four types of rubber impression materials compared with time of pour and a repeat pour of models. J Prosthet Dent. 1985 Apr;53(4):484-90. 46. Lacy AM, Bellman T, Fukui H, Jendresen MD. Time-dependent accuracy of elastomer impression materials. part I: Condensation silicones. J Prosthet Dent. 1981 Feb;45(2):209-15. 47. Lacy AM, Fukui H, Bellman T, Jendresen MD. Time-dependent accuracy of elastomer impression materials. part II: Polyether, polysulfides, and polyvinylsiloxane. J Prosthet Dent. 1981 Mar;45(3):329-33. 48. Hung SH, Purk JH, Tira DE, Eick JD. Accuracy of one-step versus two-step putty wash addition silicone impression technique. J Prosthet Dent. 1992 May;67(5):583-9. 49. Mishra S, Chowdhary R. Linear dimensional accuracy of a polyvinyl siloxane of varying viscosities using different impression techniques. J Investig Clin Dent. 2010;1:37-46. 50. Valderhaug J, Floystrand F. Dimensional stability of elastomeric impression materials in custom-made and stock trays. J Prosthet Dent. 1984 Oct;52(4):514-7. 51. Rueda LJ, Sy-Munoz JT, Naylor WP, Goodacre CJ, Swartz ML. The effect of using custom or stock trays on the accuracy of gypsum casts. Int J Prosthodont. 1996 JulAug;9(4):367-73. 136 52. Dixon DL, Breeding LC, Brown JS. The effect of custom tray material type and adhesive drying time on the tensile bond strength of an impression material/adhesive system. Int J Prosthodont. 1994 Mar-Apr;7(2):129-33. 53. Thongthammachat S, Moore BK, Barco MT,2nd, Hovijitra S, Brown DT, Andres CJ. Dimensional accuracy of dental casts: Influence of tray material, impression material, and time. J Prosthodont. 2002 Jun;11(2):98-108. 54. Logozzo S, Franceschini G, Kilpelä A, Caponi M, Governi L, Blois L. A comparative analysis of intraoral 3d digital scanners for restorative dentistry. The Internet Journal of Medical Technology [Internet]. 2011;5(1). Available from: 10.5580/1b90#sthash.nKoGlwm3.dpuf. 55. Scotti R, Cardelli P, Baldissara P, Monaco C. Clinical fitting of CAD/CAM zirconia single crowns generated from digital intraoral impressions based on active wavefront sampling. J Dent. 2011 Oct 17. 56. Beuer F, Schweiger J, Edelhoff D. Digital dentistry: An overview of recent developments for CAD/CAM generated restorations. Br Dent J. 2008 May 10;204(9):505-11. 57. Strub JR, Rekow ED, Witkowski S. Computer-aided design and fabrication of dental restorations: Current systems and future possibilities. J Am Dent Assoc. 2006 Sep;137(9):1289-96. 58. Syrek A, Reich G, Ranftl D, Klein C, Cerny B, Brodesser J. Clinical evaluation of allceramic crowns fabricated from intraoral digital impressions based on the principle of active wavefront sampling. J Dent. 2010 Jul;38(7):553-9. 59. Fasbinder DJ. Digital dentistry: Innovation for restorative treatment. Compend Contin Educ Dent. 2010;31 Spec No 4:2-11. 60. Birnbaum NS, Aaronson HB. Dental impressions using 3D digital scanners: Virtual becomes reality. Compend Contin Educ Dent. 2008 Oct;29(8):494, 496, 498-505. 61. Birnbaum NS, Aaronson HB, Stevens C, Cohen B. 3D digital scanners: A high-tech approcah to more accurate dental impressions. Inside Dentistry. 2009 4/2/2009;5(4):70-7. 62. Seelbach P, Brueckel C, Wostmann B. Accuracy of digital and conventional impression techniques and workflow. Clin Oral Investig. 2012 Oct 21. 63. Plourde J, Harsono M, Fox L, Hill TJ, Finkelman M, Kugel G. Marginal and internal fit of E4D CAD/CAM all-ceramic crowns. J Dent R. 2011;90(A):Abstract # 638. 137 64. Matthes J, Silva N, Wolff MS, Witek L, Zavanelli RA, Conciatori L, et al. Marginal microscopic analysis of CAD/CAM all ceramic crown. J Dent R. 2011;90(A):Abstract # 3200. 65. Denry I, Holloway J. Ceramics for dental applications: A review. Materials [Internet]. 2010;3(1):11 January 2010,351-368. Available from: www.mdpi.com/journal/materials. 66. Holand W, Schweiger M, Watzke R, Peschke A, Kappert H. Ceramics as biomaterials for dental restoration. Expert Rev Med Devices. 2008 Nov;5(6):729-45. 67. Conrad HJ, Seong WJ, Pesun IJ. Current ceramic materials and systems with clinical recommendations: A systematic review. J Prosthet Dent. 2007 Nov;98(5):389-404. 68. Kelly JR. Dental ceramics: Current thinking and trends. Dent Clin North Am. 2004 Apr;48(2):viii, 513-30. 69. Holden JE, Goldstein GR, Hittelman EL, Clark EA. Comparison of the marginal fit of pressable ceramic to metal ceramic restorations. J Prosthodont. 2009 Dec;18(8):645-8. 70. Lee KB, Park CW, Kim KH, Kwon TY. Marginal and internal fit of all-ceramic crowns fabricated with two different CAD/CAM systems. Dent Mater J. 2008 May;27(3):422-6. 71. McLaren EA, Terry DA. CAD/CAM systems, materials, and clinical guidelines for all-ceramic crowns and fixed partial dentures. Compend Contin Educ Dent. 2002 Jul;23(7):637-53. 72. Baig MR, Tan KB, Nicholls JI. Evaluation of the marginal fit of a zirconia ceramic computer-aided machined (CAM) crown system. J Prosthet Dent. 2010 Oct;104(4):21627. 73. Stappert CF, Chitmongkolsuk S, Silva NR, Att W, Strub JR. Effect of mouth-motion fatigue and thermal cycling on the marginal accuracy of partial coverage restorations made of various dental materials. Dent Mater. 2008 Sep;24(9):1248-57. 74. Miyazaki T, Hotta Y. CAD/CAM systems available for the fabrication of crown and bridge restorations. Aust Dent J. 2011 Jun;56 Suppl 1:97-106. 75. Culp L, McLaren EA. Lithium disilicate: The restorative material of multiple options. Compend Contin Educ Dent. 2010 Nov-Dec;31(9):716,20, 722, 724-5. 76. Denry I, Kelly JR. State of the art of zirconia for dental applications. Dent Mater. 2008 Mar;24(3):299-307. 138 77. Sailer I, Feher A, Filser F, Gauckler LJ, Luthy H, Hammerle CH. Five-year clinical results of zirconia frameworks for posterior fixed partial dentures. Int J Prosthodont. 2007 Jul-Aug;20(4):383-8. 78. Fahmy NZ. Influence of veneering materials on the marginal fit and fracture resistance of an alumina core system. J Prosthodont. 2011 Jan;20(1):45-51. 79. Fradeani M, Redemagni M. An 11-year clinical evaluation of leucite-reinforced glassceramic crowns: A retrospective study. Quintessence Int. 2002 Jul-Aug;33(7):503-10. 80. Heintze SD, Rousson V. Fracture rates of IPS empress all-ceramic crowns--a systematic review. Int J Prosthodont. 2010 Mar-Apr;23(2):129-33. 81. El-Mowafy O, Brochu JF. Longevity and clinical performance of IPS-empress ceramic restorations--a literature review. J Can Dent Assoc. 2002 Apr;68(4):233-7. 82. Murgueitio R, Bernal G. Three-year clinical follow-up of posterior teeth restored with leucite-reinforced ips empress onlays and partial veneer crowns. J Prosthodont. 2012 Jul;21(5):340-5. 83. Stappert CF, Dai M, Chitmongkolsuk S, Gerds T, Strub JR. Marginal adaptation of three-unit fixed partial dentures constructed from pressed ceramic systems. Br Dent J. 2004 Jun 26;196(12):766-70. 84. Marquardt P, Strub JR. Survival rates of IPS empress 2 all-ceramic crowns and fixed partial dentures: Results of a 5-year prospective clinical study. Quintessence Int. 2006 Apr;37(4):253-9. 85. Tysowski GW. The science behind lithium disilicate: A metal-free alternative. Dentistry Today 03/01/2009. 86. Aboushelib MN, de Jager N, Kleverlaan CJ, Feilzer AJ. Effect of loading method on the fracture mechanics of two layered all-ceramic restorative systems. Dent Mater. 2007 Aug;23(8):952-9. 87. Guess PC, Zavanelli RA, Silva NR, Bonfante EA, Coelho PG, Thompson VP. Monolithic CAD/CAM lithium disilicate versus veneered Y-TZP crowns: Comparison of failure modes and reliability after fatigue. Int J Prosthodont. 2010 Sep-Oct;23(5):434-42. 88. Schmitter M, Mueller D, Rues S. Chipping behaviour of all-ceramic crowns with zirconia framework and CAD/CAM manufactured veneer. J Dent. 2012 Feb;40(2):15462. 89. Etman MK. Confocal examination of subsurface cracking in ceramic materials. J Prosthodont. 2009 Oct;18(7):550-9. 139 90. Wiedhahn K. From blue to white: New high-strength material for cerec--IPS e.max CAD LT. Int J Comput Dent. 2007 Jan;10(1):79-91. 91. Pjetursson BE, Sailer I, Zwahlen M, Hammerle CH. A systematic review of the survival and complication rates of all-ceramic and metal-ceramic reconstructions after an observation period of at least 3 years. part I: Single crowns. Clin Oral Implants Res. 2007 Jun;18 Suppl 3:73-85. 92. Guess PC, Strub JR, Steinhart N, Wolkewitz M, Stappert CF. All-ceramic partial coverage restorations--midterm results of a 5-year prospective clinical splitmouth study. J Dent. 2009 Aug;37(8):627-3. 93. Etman MK, Woolford MJ. Three-year clinical evaluation of two ceramic crown systems: A preliminary study. J Prosthet Dent. 2010 Feb;103(2):80-9. 94. Fasbinder DJ, Dennison JB, Heys D, Neiva G. A clinical evaluation of chairside lithium disilicate CAD/CAM crowns: A two-year report. J Am Dent Assoc. 2010 Jun;141 Suppl 2:10S-4S. 95. Christensen GJ. Marginal fit of gold inlay castings. J Prosthet Dent. 1966 MarApr;16(2):297-305. 96. Rahme HY, Tehini GE, Adib SM, Ardo AS, Rifai KT. In vitro evaluation of the "replica technique" in the measurement of the fit of procera crowns. J Contemp Dent Pract. 2008 Feb 1;9(2):25-32. 97. Holst S, Karl M, Wichmann M, Matta RE. A new triple-scan protocol for 3D fit assessment of dental restorations. Quintessence Int. 2011 Sep;42(8):651-7. 98. Laser desing inc and GKS [Internet]. Available from: http://www.laserdesign.com/resources_and_downloads/faq/. 99. Marks P. Capturing a competitive edge through digital shape sampling & processing (DSSP). SME Blue Book Series ed. Society of Manufacturing Engineers; 2005. 100. Geomagic [Internet]. Available from: http://dl.geomagic.com/media/marketing/2012_Brochures/MASTER12_29_11_QQP_A4_English_eVersion.pdf. 101. Leong DK, Chai JY, Gilbert JL. Marginal fit of machine-milled and cast titanium single crowns. Northwest Dent Res. 1994 Winter;4(2):13-4. 102. Harris IR, Wickens JL. A comparison of the fit of spark-eroded titanium copings and cast gold alloy copings. Int J Prosthodont. 1994 Jul-Aug;7(4):348-55. 140 103. Yeo IS, Yang JH, Lee JB. In vitro marginal fit of three all-ceramic crown systems. J Prosthet Dent. 2003 Nov;90(5):459-64. 104. Stappert CF, Denner N, Gerds T, Strub JR. Marginal adaptation of different types of all-ceramic partial coverage restorations after exposure to an artificial mouth. Br Dent J. 2005 Dec 24;199(12):779,83; discussion 777. 105. Walker MP, Petrie CS, Haj-Ali R, Spencer P, Dumas C, Williams K. Moisture effect on polyether and polyvinylsiloxane dimensional accuracy and detail reproduction. J Prosthodont. 2005 Sep;14(3):158-63. 106. Klooster J, Logan GI, Tjan AH. Effects of strain rate on the behavior of elastomeric impression. J Prosthet Dent. 1991 Sep;66(3):292-8. 107. Eames WB, Sieweke JC. Seven acrylic resins for custom trays and five putty-wash systems compared. Oper Dent. 1980 Autumn;5(4):162-7. 108. Lewinstein I. The ratio between vertical and horizontal changes of impressions. J Oral Rehabil. 1993 Jan;20(1):107-14. 109. Kenyon BJ, Hagge MS, Leknius C, Daniels WC, Weed ST. Dimensional accuracy of 7 die materials. J Prosthodont. 2005 Mar;14(1):25-31. 110. Ragain JC, Grosko ML, Raj M, Ryan TN, Johnston WM. Detail reproduction, contact angles, and die hardness of elastomeric impression and gypsum die material combinations. Int J Prosthodont. 2000 May-Jun;13(3):214-20. 111. Miyazaki T, Hotta Y, Kunii J, Kuriyama S, Tamaki Y. A review of dental CAD/CAM: Current status and future perspectives from 20 years of experience. Dent Mater J. 2009 Jan;28(1):44-56. 112. Shannon AJT, Qian F, Tan P, Gratton DG. In vitro horizontal marginal adaptation comparison of CAD/CAM zirconium copings. J Dent R. 2008;87(A):Abstract #0188. 113. Tsitrou EA, Northeast SE, van Noort R. Brittleness index of machinable dental materials and its relation to the marginal chipping factor. J Dent. 2007 Dec;35(12):897902. 114. Giannetopoulos S, van Noort R, Tsitrou E. Evaluation of the marginal integrity of ceramic copings with different marginal angles using two different CAD/CAM systems. J Dent. 2010 Dec;38(12):980-6. 115. Cohen A, Laviv A, Berman P, Nashef R, Abu-Tair J. Mandibular reconstruction using stereolithographic 3-dimensional printing modeling technology. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Nov;108(5):661-6. 141 116. Bill JS, Reuther JF, Dittmann W, Kubler N, Meier JL, Pistner H, et al. Stereolithography in oral and maxillofacial operation planning. Int J Oral Maxillofac Surg. 1995 Feb;24(1 Pt 2):98-103. 117. Ogledzki M, Wenzel K, Doherty E, Kugel G. Accuracy of 3M-brontes stereolithography models compared to plaster models. J Dent R. 2010;89(A):Abstract #1060. 118. Moore W, Dunne P, Doherty E, Nelson M, Finkelman M, Kugel G. Margin characteristics of PFM crown copings fabricated on stereolithography models. 3M ESPE Web site: 3MESPE com [Internet]. Available from: http://multimedia.3m.com/mws/mediawebserver?mwsId=SSSSSufSevTsZxtUO8_xnYt9 evUqevTSevTSevTSeSSSSSS--. 119. Lava chairside oral scanner C.O.S. [Internet]. Technical Datasheet: 3M ESPE; 2009. Available from: www.multimedia.3m.com. 120. Hart D, Lammerding J, Rohaly J, inventors; 3-D imaging system. US patent 2004/0155975 A1. 2004 Aug 12. 121. Ender A, Mehl A. Full arch scans: Conventional versus digital impressions--an invitro study. Int J Comput Dent. 2011;14(1):11-2. 122. Van der Meer WJ, Andriessen FS, Wismeijer D, Ren Y. Application of intra-oral dental scanners in the digital workflow of implantology. PLoS One [Internet]. 2012 2012 August 22;7(8). Available from: http://dx.crossref.org/10.1371%2Fjournal.pone.0043312. 123. Guth JF, Keul C, Stimmelmayr M, Beuer F, Edelhoff D. Accuracy of digital models obtained by direct and indirect data capturing. Clin Oral Investig. 2012 Jul 31. 124. Balakrishnama S, Wenzel K, Bergeron J, Ruest C, Reusch B, Kugel G. Dimensional repeatabilty from the LAVA COS 3D intra-oral scanning system. J Dent R. 2009;88(A):Abstract #2951. 125. Hirayama H, Chang YC, Kugel G, Kang KH, Finkelman M. Fit of zirconia copings generated from a digital impression technique and conventional impression technique. 3M ESPE Web site: 3MESPE com [Internet]. Available from: http://multimedia.3m.com/mws/mediawebserver?mwsId=SSSSSufSevTsZxtUO8_xnYtB evUqevTSevTSevTSeSSSSSS--. 126. Chen Y, Liang CP, Liu Y, Fischer AH, Parwani AV, Pantanowitz L. Review of advanced imaging techniques. J Pathol Inform [Internet]. 2012 2012 May 28;3(22). Available from: http://dx.crossref.org/10.4103%2F2153-3539.96751. 142 127. Fujimoto JG. Optical coherence tomography for ultrahigh resolutionin vivoimaging. Nature Biotechnology [Internet]. 2003 31 October 2003;21. Available from: 10.1038/nbt892. 128. Swanson EA, Huang D, Hee MR, Fujimoto JG, Lin CP, Puliafito CA. High-speed optical coherence domain reflectometry. Opt Lett. 1992 Jan 15;17(2):151-3. 129. Quadling H, Quadling M, Blair A, inventors; Laser digitizer system for dental applications. US patent 2010/0060900 A1. 2010 Mar 11. 130. Kugel G, Beyarl M, Iamfon HA, Harsono M, Fox L, Plourde J, et al. Marginal/internal crown fit evaluation of CAD/CAM versus press-laboratory all ceramic crown. J Dent R. 2007;86(A):Abstract # 1366.