ProductInformation
EMB AGAR (LEVINE)
Product Number E5274
Product Description
EMB Agar (Levine) is recommended for the
isolation, enumeration or differentiation of members
of Enterobacteriaceae. EMB Agar (Levine) was
developed by Levine and is used for the
differentiation of Escherichia coli and Enterobacter
aerogenes. It is also used for the rapid identification
of Candida albicans. Eosin-Y and methylene blue
make the medium slightly selective and inhibit some
gram -positive bacteria. Both of these two dyes
differentiate between lactose fermenters and
nonfermenters. Some gram-positive bacteria such
as faecal Streptococci, grow on this medium and
form pinpoint colonies.
color) and to suspend the precipitate which is an
essential part of the medium.
Gelling
Firm.
Components
Item
Peptic Digest of Animal Tissue
Dipotassium Phosphate
Lactose
Eosin-Y
Methylene Blue
Agar
Color and Clarity
Reddish-purple colored,
slightly opalescent gel
having a greenish cast
with finely dispersed
precipitate forms in petri
plates.
Cultural Response
Cultural chacteristics
observed after 24-48
hours at 35-37°C.
g/L
10.00
2.00
10.00
0.40
0.065
15.00
Final pH (at 25°C) 7.1 ± 0.2
Precautions and Disclaimer For laboratory use
only. Not for drug, household or other uses.
Preparation Instructions
Suspend 37.5 grams of EMB Agar (Levine) in 1000
mls of distilled water. Boil to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs.
pressure (121°C) for 15 minutes. Avoid overheating.
Cool to 50°C and shake the medium in order to
oxidize the methylene blue (i.e. restore its blue
Storage
Store the dehydrated medium at 24°C and the
prepared medium at 2-8° C. Store the medium away
from light to avoid photooxidation.
Product Profile
Appearance
Light purple colored,
homogeneous, free
flowing powder.
Organisms
Growth
Escherichia coli
luxuriant
Pseudomonas aeruginosa luxuriant
Salmonella typhimurium
luxuriant
Enterobacter aerogenes good
Staphylococcus aureus
inhibited
Color
blue-black
colorless
colorless
pink
none
References:
1. Levine, M., (1918). J. Infect. Dis. 23,43.
2. Levine, M., (1921). Bull. 62. Iowa State College
Engr. Exp. Station.
3. Standard Methods for the Examination of Water
and Wastewater, (1985).Greenberg, A.E., et. al.,
th
eds. 16 Edition. APHA. Washington, D.C.
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Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser
must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of
the invoice or packing slip.