One Gel, One Array, One Software
CEQ™ 8000 STR ANALYSIS
Tandem repeat regions in DNA sequences are
widespread throughout the human, plant and
microbe genomes and show sufficient variability
among individuals that they have become
important in genetic mapping and linkage
analysis. These tandemly repeated regions of
DNA are typically classified into several groups
depending on the size of the repeat region.
Minisatellites (variable number of tandem
repeats, VNTRs) have core repeats with 9-80 bp,
while microsatellites (short tandem repeats, STRs)
contain 2-7 bp repeats. STR markers are very
abundant and well-distributed throughout the
genomes, located approximately every 10-15 kb.
Identification of Tandem Repeats Sequencing
Genomic tandem repeats are identified by
dye terminator cycle sequencing (DTCS)
(Figure 1).
Figure 1. Identification of genomic tandem repeats.
Automated Tandem Repeat Analysis Fragment Analysis
Tandem repeats have become one of the most
widely used genomic markers due to their high
degree of heterozygosity and simple methodology
– PCR* amplification, fragment sizing and locus /
allele scoring. The CEQ™ 8000 Genetic Analysis
System provides accurate and high precision sizing
of the amplified fragments for automated allele
and locus identification. It identifies alleles
even in the presence of polymerase artifacts
such as stutter and plus A peaks.
The electropherograms may be viewed
individually, overlaid or stacked to facilitate
data comparison. CEQ™ DNA Size Standard400 or Size Standard-600 are used for fragment
sizing (Figure 2).
Figure 2. Automated tandem repeat analysis.
CEQ™ DTCS Quick Start Kit
Figure 3. STRs may be identified by
size allowing alleles to be identified.
Figure 4. Locus Tag are automatically generated
using the integrated Binning software.
Figure 5.
Exported STR
genotype table.
The STR genotypes can be exported in spreadsheet format
(Figure 5) for linkage/association studies or used for automated
analysis of cancer loss of heterozygosity (LOH) / microsatellite
instability (MI) or phylogenetic analysis (Figure 6).
Figure 6. The results are easily used
for phylogenetic analysis.
Ordering Information:
Reagents for Automated DNA Sequencing:
CEQ™ Supplies
608120
CEQ DTCS Quick Start Kit
608087
608000
CEQ™ DTCS Kit
CEQ™ DNA Separation Capillary Array,
33cm × 75 mm
608082
CEQ™ Sample Loading Buffer (SLS), 6.0 ml
Reagents for Short Tandem Repeat Analysis:
608010
CEQ™ Separation Gel 1
608098
CEQ™ DNA Size Standard Kit – 400 (for 60-400 bp)
608012
CEQ™ Sequencing Separation Buffer, 4/pk
608095
CEQ DNA Size Standard Kit – 600 (for 60-600 bp)
609844
CEQ™ Separation Buffer Plates, Nonsterile,
without lids, 300 µl/well, 100/pk
609801
Sample Microtiter Plates, 25/pk, V-bottom,
Thermal cycler compatible, 200 µl/well
™
™
For Labeled Primers contact your local Beckman Coulter sales representative.
Hardware and Software
285501
CEQ™ 8000 Genetic Analysis System, 110/230 V
With CEQ™ 8000 Software for Sequencing and
Fragment Analysis
285590
CEQ™ 8000 Migration Package (from CEQ™ 2000XL)
* The PCR process is covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffman-La Roche, Ltd.
All trademarks are the property of their respective owners.
Developing innovative solutions in genetic
analysis, drug discovery, and instrument systems.
For Research Use Only. Not for use in diagnostic procedures.
© 2014 AB SCIEX. SCIEX is part of AB Sciex. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license.
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