The Dolomite Centre Ltd
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The Dolomite Centre Ltd
The components that make up the system discussed in this datasheet are listed below.
Resealable Chip Interface with Top and Bottom Chips
This set of components allows the Pressure-Driven Resealable Chip Interface System to be assembled in a range of configurations.
Part No. Part Description #
3000305 Resealable Chip Interface 1
3200069
3200070
3200071
3200072
Manual Injection Top Chip
Manual Multi-Injection Top Chip
Top Chip, 1-in, 1-out
Top Chip, 4-in, 1-out
3200120
3000478
Top Chip 3-in, 1-out
Large Well Base Chip
3000343 Blank Base Chip 1
3200177 Linear Connector 4-way 2
3200177
3200017
3200178
3200206
3200197
3200128
P Pump system for Fluid Supply
Two P-Pump Remote Basic units are shown, with Remote Reservoirs. One reservoir is fitted with a 3-way lid, to allow up to 3 different fluids to be delivered to the Pressure-
Driven Resealable Chip Interface System. The second Reservoir has a single fluid port, and is optimized for agitation of suspensions using a “tapping” method. It can also be used for delivery of larger quantities of fluid, for example for wash solutions.
Part No. Part Description #
Mitos P-Pump Remote Basic
Mitos P-Pump Vessel Holders Kit
Mitos P-Pump Remote Chamber 30
Mitos P-Pump Remote Chamber 30 with 3-way Accessories
USB to RS232 Adaptor Cable
Mitos P-Pump Compressor 6bar EU (230V/50Hz)
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2
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3200293
3200050
3200064
3000056
3200087
Flow sensors
FEP Tubing, 1/16" x 0.5mm, 10 metres
Plug FEP (pack of 10)
2-way In-line Valve
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4
The P Pump system can be upgraded to allow direct control of flow rate, if this is required.
We suggest that a set of 3 sensors and interfaces are used, to give a range of options for experiments.
Part No. Part Description #
3200200
3200244
Mitos Sensor Interface
Mitos Flow Rate Sensor, mixed pack of 3
Microscope system
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4
Dolomite offers two microscope systems to allow imaging and recording of fluid flow in the
Pressure-Driven Resealable Chip Interface System. A low cost digital microscope can be used if it is not necessary to capture fast moving events. A high speed camera and microscope option is offered, to allow image capture at up to 1,000 frames per second.
Part No. Part Description #
Digital Microscope
High Speed Camera and Microscope System
1
1
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The Dolomite Centre Ltd
The Pressure-Driven Resealable Chip Interface System is ideal for applications where a sealed microfluidic device is not suitable, such as biosensor testing, cell culture and analysis, diaelectrophoresis experiments, impedance detection, and interfacing with silicon chips and sensors. The Resealable Chip offers customization to the n th degree. Its versatility stems from the scientist being able to easily take the chip apart and put it back together over and over again, this makes it a tool with many uses that are appropriate across a variety of applications. Examples of Pressure-Driven Resealable Chip Interface
System applications include:
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Where access to the chip surface is required, eg to print or spot biologically active materials and then perfuse fluids over those materials.
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Where cleaning or replacement of some parts of the system are required between experiments
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Where one part of the device is provided by the user, for example a sensor or an array of cells, which must be exposed to fluids by assembling it into the Pressure-
Driven Resealable Chip Interface System.
Reagents, sensors, biosensors or cells can be deposited on the chip base layer. The chip base layer is typically glass, quartz, or polymer. Fluids are then flowed through channels in the gasket layer over the deposited sensors or reagents. The depth of the channels in the gasket is typically between 100-500 microns.
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Low profile allows viewing with a microscope
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Excellent chemical resistance
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Easy to clean
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Eight fluidic and eight electrical connections
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Allows deposition of reagents, sensors or cells onto channel surface
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Tool - free system set up
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The Dolomite Centre Ltd
Specification
Fluid inputs
Fluid outputs
Flow rates
Wetted materials
1-4
1-4
70nl-5ml/min
Glass, PMMA, PDMS, (platinum/gold electrodes)
10kg
0 – 10bar (0 – 145psi) gauge
Weight and size
Pressure range
The diagram below shows a complete system which allows the scientist to deliver a range of fluids into the Pressure-Driven Resealable Chip Interface System. Two P-Pumps are used in this system. The Mitos P-Pump Remote Basic is recommended, as it allows use of a remote reservoir. These reservoirs can be placed in a chamber for temperature control and to ensure a sterile environment. The reservoir contents can also be agitated if required, for example to maintain cells or beads in suspension. The fluidic schematic is shown below.
3200206 Mitos P-Pump Remote Chamber 30 with 3-way Accessories
4 3200244 Mitos Flow Rate Sensor, mixed pack of 3
4 3200200 Mitos Sensor Interface
3200177 Mitos P-Pump Remote Basic
4x 3200087 2-Way In-line Valve
3200128 Mitos P-Pump
Compressor 6bar EU
(230V/50Hz)
3000305 Resealable Chip Interface
2 3000024 Linear Connector 4-way
3200177 Mitos P-Pump Remote Basic 3200017 Mitos P-Pump Vessel Holders Kit
3200178 Mitos P-Pump Remote Chamber 30
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It should be noted that the system is entirely modular and options such as the microscopes can be removed if necessary. Please contact Dolomite to customize your system.
Resealable Chip Interface (Part No. 3000305)
The Resealable Chip Interface is used in combination with microfluidic chips that can be opened and resealed. The holder clamps the layers together and provides eight fluidic and eight electrical connections to the chip. Two linear connectors 4-way
(Part No. 3000148) are included. Reagents, sensors, biosensors or cells can be deposited on the chip base layer. The chip base layer material is typically glass, quartz, or polymer.
Features and benefits:
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Excellent access for optics
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Excellent chemical resistance
Easy to clean
 8 fluidic/electrical connections
 Allows deposition of reagents, sensors or cells onto channel surface
Manual Injection Top Chip (Part No. 3200069)
The Manual Injection Top Chip is designed for use with the Resealable Chip Interface (Part No. 3000305) and a
Base Chip. Liquids can be dispensed into the 10mm x10mm x 3mm reservoir using a pipette. The liquid will only come into contact with a 10mm x 10mm area on a base chip. Applications include surface modification of the base chip and deposition of cells onto the surface of the base chip.
Features and benefits:
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Channel size: 10mm x 10mm
 2 additional channel allow purging the area with gasses
Chip material: PMMA
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Gasket material: PDMS
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The Dolomite Centre Ltd
Manual Multi-Injection Top Chip (Part No. 3200070)
The Manual Multi-Injection Top Chip is designed for use with the Resealable Chip Interface (Part No. 3000305) and a Base Chip. Liquids can be dispensed into the 25 reservoirs which are 1.5mm diameter x 3mm high using a pipette. The liquids will only come into contact with an array of 1.5mm diameter circles on the Base Chip.
Applications include surface modification of the base chip and deposition of cells into the surface of the base chip.
Features and benefits:
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25 reservoirs with a diameter of 1.5mm
2 additional channel allow purging the area with gasses
Chip material: PMMA
Gasket material: PDMSble chip INTERFACE with p-pump
Top Chip, 1-in, 1-out (Part No. 3200071)
The Top Chip is designed for use with the Resealable
Chip Interface (Part No. 3000305) and a Base Chip. It features an integrated elastomer gasket which seals with the Base Chip. By using the Linear Connector 4-way (Part
No. 3000024) liquid can be pumped into the chip and across the surface of the Base Chip.
Features and benefits:
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Channel depth: 100µm
Channel width (central region): 11mm
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Chip material: PMMA
Gasket material: PDMS
Top Chip, 4-in, 1-out (Part No. 3200072)
The Top Chip is designed for use with the Resealable
Chip Interface (Part No. 3000305) and a Base Chip. It features an integrated elastomer gasket which seals with the Base Chip. By using the Linear Connector 4-way (Part
No. 3000024) liquid can be pumped into the chip and across the surface of the Base Chip. The chip features four input streams which can be connected with one output stream. This allows the positions of the streams to be controlled and the potential generation of a concentration gradient.
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The Dolomite Centre Ltd
Features and benefits:
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Channel depth: 100µm
Channel width (central region): 11mm
Channel width input stream: 0.5mm
Chip material: PMMA
Gasket material: PDMS
Top Chip 3-in, 1-out (Part No. 3200120)
The Top Chip, 3-in, 1-out is designed for use with the
Resealable Chip Interface (Part No. 3000305) and a Base
Chip. It features an integrated elastomer gasket which seals with the Base Chip. By using the Linear Connector 4-way (Part
No. 3000024) liquid can be pumped into the chip and across the surface of the Base Chip. The chip features three input streams which can be connected with one output stream.
This allows the positions of the streams to be controlled and the potential generation of a concentration gradient. There is also a straight 1-in, 1-out channel.
Features and benefits:
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 Channel depth: 100µm
 Channel width (central region): 3mm
Chip material: PMMA
Gasket material: PDMS
Large Well Base Chip (Part No. 3000478)
The Large Well Base Chip can be used in combination with the
Resealable Chip Interface (Part No. 3000305) and a Top Chip
(Part Nos. 3200069-72). The chip is made from glass and requires a Top Chip to create a fluidic seal. A central square area (9mm x 9mm) is etched to a depth of 100µm providing a recessed area in the fluid flow path. Materials or samples can be deposited in the recess prior to assembly in the holder.
Features and benefits:
 Surface Roughness of glass surface and etched features: 5nm
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Chip material: Glass
Blank Base Chip (Part No. 3000343)
The Blank Base Chip can be used in combination with the
Resealable Chip Interface (Part No. 3000305) and a Top Chip
(Part Nos. 3200069-72). The chip is made from glass and requires a Top Chip to create a fluidic seal. There are no
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The Dolomite Centre Ltd etched features on this chip. Examples of use include the deposition of thin layers onto the surface of the chip prior to assembly in the holder for fluidic experiments.
Linear Connector 4-way (Part No. 3200177)
The Linear Connector 4-way allows fast, reliable and efficient connection between Dolomite chips and 1.6mm O.D. tubing. The Linear Connector works with chips that are 4mm thick, 15mm wide and also have a chip header in place. It can be used together with the Linear Connector Seal (Part
No. 3000021) .
Features and benefits:
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Number of ports: 4
 Small footprint
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Operating pressure: 30bar
 Operating temperature range of seal: -15°C to 150°C
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Quick and easy to connect/disconnect
 Low dead volume
Wetted material: glass, PTFE and perfluoroelastomer
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Excellent chemical compatibility
Mitos P-Pump Remote Basic (Part No. 3200177)
The Mitos P-Pump Remote Basic connects to a PC via RS232 serial interface allowing data logging and control using free PC software. For PCs that do not support an RS232 serial interface, Dolomite offers a
USB adapter (Part No. 3200197). All the other features of the pump are the same as for the Mitos
P-Pump Remote (Part No. 3200177) including pulseless pumping performance and easy to use pneumatic fitting.
Features and benefits:
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 Fast response time
 Wide pressure range (0 - 10bar) enabling
 use with systems of high fluidic resistance
Flexible - accommodates a wide range of fluid vessels (standard and nonstandard)
Quick and easy to use
Works with laboratory N2 or Ar supply, gas bottle or compressor
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The Dolomite Centre Ltd
Mitos P-Pump Vessel Holders Kit (Part No. 3200017)
The Mitos P-Pump Vessel Holder Kit contains 4 vessel holders to enable the Mitos P-Pump (Part No. 3200016) and the Mitos
P-Pump Basic (Part No. 3200175) to be used with a wide range of standard and non-standard fluid vessels. These include centrifuge tubes, autosampler vials, scintillation vials, microreaction vials and other general purpose vials. The vessel holders can be easily positioned inside the pressure chamber and are quick to change.
Features and benefits:
 Accommodates a wide range of standard and nonstandard fluid vessels
 Ensures the Mitos P-Pump can be used with preferred vessel
 Minimizes sample waste by pumping directly from the container
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Enables use of micro vials for low sample volumes
 Material: stainless steel
Available in 4 sizes
Mitos P-Pump Remote Chamber 30 (Part No. 3200178)
The Mitos P-Pump Remote Chamber 30 is a 0 - 10bar lockable glass pressure chamber for use with the Mitos P-
Pump Remote (Part No. 3200176) and the Mitos P-Pump
Remote Basic (Part No. 3200177). The Mitos P-Pump
Remote Chamber 30 connects securely to a Mitos P-
Pump Remote via a pneumatic fitting located on the base and can contain fluid samples from 100µl to 30ml. For applications requiring temperature control and stirring, the remote chamber can be placed onto a hotplate, magnetic stirrer or placed in an incubator.
Features and benefits:
 Sample volumes from 100µl to 30ml
 Option for heating and magnetic stirring
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Easy to view sample liquid levels
 Quick and easy fitting to connect to Mitos P-Pump Remote
 Excellent chemical compatibility
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The Dolomite Centre Ltd
Mitos P-Pump Remote Chamber 30 with 3-way Accessories (Part No. 3200206)
The Mitos P-Pump Remote Chamber with 3-way Accessories combines the Mitos P-
Pump Remote Chamber 30 (Part No. 3200178) with the Mitos P-Pump 3-way Chamber
Lid (Part No. 3200044) and Mitos P-Pump 3-way Vessel Holder Kit (Part No. 3200045) providing a low cost method of delivering 3 reagents to a microfluidic system with pulseless pressure pumping technology.
Features and benefits:
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Provides a low cost solution for 3-channel pumping with the Mitos P-Pump
Quick to open and close thanks to push-androtate mechanism
3-way Vessel Holder and micro tubes for easy pumping from 3 locations
USB to RS232 Adaptor Cable (Part No. 3200197)
For PCs that do not have an RS232 serial interface, Dolomite offers a RS232 to USB
Adaptor Module allowing users to connect their Mitos P-Pump Basic (Part No.
3200175) or Mitos P-Pump Remote Basic
(Part No. 3200177) to a PC.
Mitos P-Pump Compressor 6bar EU (230V/50Hz) (Part No. 3200128)
Delivering oil-free and filtered air, the Mitos Compressor
6bar allows the Mitos P-Pumps to be used in areas where no compressed air supply or gas bottle are available. Operating at 230V/50Hz the portable compressor can be easily connected to the pump using the Pneumatic Connector
Kit (Part No. 3200034).
Features and benefits:
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3 litre air reservoir
 1.8m power cable
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On demand regulated compressed air
 Compact and lightweight (5kg)
In-built pressure gauge and regulator
Automatic cut off to prevent overheating
Quiet operation
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The Dolomite Centre Ltd
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Clean, oil and water-free supply
FEP Tubing, 1/16" x 0.5mm, 10 metres (Part No. 3200064)
Fluid Tubes can be used to connect Dolomite's
Multiflux connectors to the Mitos P-Pump (Part No.
3200016), Mitos Syringe Pumps (Part No.
3200057 and 3200046), other Multiflux Connectors and Interfaces or modules that have a 1/4 - 28 thread using an end fitting and ferrule. The FEP tubes are translucent offering a better optical clarity and accuracy.
Features and benefits:
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FEP material
 Pipe length: 10m
Internal diameter: 0.5mm
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Max. operating pressure: 130bar
 Operating temperature range: -51°C to 50°C
 Quick and easy to set up and connect
 Excellent chemical resistance
Plug FEP (pack of 10) (Part No. 3000056)
The Plug FEP is used in conjunction with the
Circular Connector (Part No. 3000051) and
Linear Connector 4-way (Part No. 3000024). The
Plug FEP enables users to block flow out of a port on a chip or connector. Available in a pack of
10.
2-way In-line Valve (Part No. 3200087)
The 2-way In-line Valve provides an easy-to-use solution to quickly stop flow streams.
Features and benefits:
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Material: ETFE (similar to PTFE)
Excellent chemical resistance
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Biocompatible, all polymer flow-path
 Low internal volume: 2.5µl
Supplied with two fittings that connect to 1/16" OD tubing
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The Dolomite Centre Ltd
Mitos Sensor Interface (Part No. 3200200)
The Mitos Sensor Interface provides a cost-effective alternative to the Mitos Sensor Display featuring a circular multi-pin interface cable for connection to the Mitos P-Pump range allowing flow rate data to be read by the Mitos P-Pump software.
Features and benefits:
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Easy to use, just connect and switch on
Dimensions: 20.5mm (length) x 82mm (width) x 55mm (height)
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Interchangeable flow rate sensors to provide wide range of flow rates
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Excellent chemical resistance
Compatible with all Mitos Flow Rate Sensors (Part Nos.3200096 - 3200100)
Mitos Flow Rate Sensor, mixed pack of 3 (Part No. 3200244)
Dolomite`s Mitos Flow Rate Sensors are available in a mixed pack of 3. Please select the Flow Rate Sensors you require from the list below and specify your selection when placing the order.
Dolomite's range of Flow Rate Sensors includes:
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 Part No. 3200096 - Flow Rate Sensor 0.2 - 5ml/min
 Part No. 3200097 - Flow Rate Sensor 30 - 1000µl/min
Part No. 3200098 - Flow Rate Sensor 1 - 50 µl/min
Part No. 3200099 - Flow Rate Sensor 0.4 - 7µl/min
Part No. 3200100 - Flow Rate Sensor 70 - 1500nl/min
Digital Microscope (Part No. 3200293)
The Digital Microscope is a compact solution for capturing high quality still and video images. The innovative design replaces the traditional eyepiece with an LCD screen for easy and confortable viewing of microfluidic experiments.
Easy to use, this microscope provides high powers from 40x up to 400x (up to 1600x with digital zoom). It is also ideally suited for tailored adjust the illumination to maximize the image quality of the microfluidic chips.
The microscope is supplied with 4x, 10x and 40x objective lens, LCD Monitor, top and bottom illuminators, USB cable and SD Card Slot, AC Adapter, Dust Cover and Carrying Case as well as Digital Camera and Filter Wheel- built in.
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The Dolomite Centre Ltd
Features and benefits:
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LCD display with 4x Digital Zoom for easy and confortable viewing of your microfluidic experiments without any need of PC connections
High quality image with a built-in digital camera (2MP)
Top and bottom LED illumination
Easily connected to a PC using USB cable
Easy, portable and compact design
High Speed Camera and Microscope System (Part No. 3200050)
The High Speed Camera and Microscope System is a high quality and flexible solution for general microscopy and high speed image capture in microfluidic applications. It features an easy-to-use microscope with wide zoom range and long working distance.The microscope stage is designed to accommodate all types of microfluidic chips and enables users to quickly locate and observe the area of interest. The high speed camera integrates with the microscope and provides image capture at speeds of over 1000fps via FireWire link to a desktop PC. Illumination is provided by a 150W illuminator with alternative fibre optic light guides for different lighting options.
Features and benefits:
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Wide zoom range
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Long working distance
Still image and high speed video capture
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View through microscope or live images on PC
 Ideal for observation of microfluidic experiments
High power illumination from above or below
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Easy to use
IP License
Dolomite is a licensee of Japa n Science and Technology Agency (“JST”) under JST’s microdroplet generation technology.
This enables our customers to purchase and use our droplet chips for R&D purposes without any restriction from this comprehensive IP family.
Contact us for more information about licensing this IP for your custom application or chip design.
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The Dolomite Centre Ltd
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