ISRA UNIVERSITY
COLLEGE OF PHARMACY
1st Semester 2012/2013. Lab. hrs: 02:00 – 05:00 Pm (Sun. &Wed.)
Lecturer:
Course Name:
Office:
Office Hours:
E-mail:
Teaching Assistant:
Dr. Khaldun Mohammad Al Azzam
Practical Analytical Chemistry and Instrumental Analysis
(501270).
Room 321, Phone: 2743
(Mon. &Wed.) 12:30 -1:30 Pm
(Sun, Tue & Thu) 10:00 -11:00 Am
khaldun.alazzam@iu.edu.jo
Ms. Rasha
Course Description:
The main objective of this lab is to introduce students to the most used techniques in
pharmaceuticals analysis. Moreover, students will conduct analysis using Classical
methods such as titration. Additionally, the students will have the chance to apply some
modern instrumental analytical methods such as spectroscopic and chromatographic
methods. At the end of the course the student must be able to discuss the principle,
operation, sensitivity, and limitations of each studied method.
Grading:
Assessment Type
Quizzes
Reports and Questions
Evaluation & Attendance
Midterm Exam
Final Exam
Total
Expected Due Date
Within the lab session
Within one week of
Laboratory session
At the beginning of each
laboratory session
To be announced later
To be announced later
100 %
1
Weight (%)
10
15
10
25
40
Experiments Schedule:
Week No.
1
2
3
4
5
6
7
8
9
10
11
Experiment
Check in, introduction, safety laboratory rules
Experiment 1: Sampling and statistical handling of data
Experiment 2: Analysis of Commercial Antacid tablets
Experiment 3: Phosphoric acid Analysis
Experiment 4: UV- spectrophotometric method for the determination of
mefenamic acid in pharmaceutical formulation
Experiment 5: UV- Visible molecular Absorption Spectroscopy -1determination of chromium concentration
Mid term Exam
Experiment 6: UV- Visible molecular Absorption Spectroscopy -1determination of paracetamol concentration
Experiment 7: Atomic emission spectroscopy- determination of sodium
using flame photometry
Experiment 8: Atomic Absorption Spectroscopy- determination of lead
concentration
Experiment 9: High Performance Liquid Chromatography (HPLC): assay
of mefenamic acid
Experiment 10: Thin Layer Chromatography (TLC) & adsorption
chromatography (theory)
FINAL EXAM
Laboratory Instructions:
- Each student is entitled of ONE absence only in all semester with acceptable excuse,
anyone exceed this limit will not be allowed to set for the final exam
- All students are requested to be at lab on time with no longer than 15 minutes
LABORATORY PRACTICES:
1. Always wear your safety goggles in the lab! Eyes are too precious to waste.
2. A lab coat to protect you and your clothing.
3. Never eat or drink in the lab!
4. Always come prepared! Students who come unprepared make more mistakes, waste
time, and often get poor (and late!!) results.
5. Know where the safety equipment is located and how it operates!
6. If you make the mess, you clean it up! This especially applies to the balances and
the balance area!
7. Operations that require a fume hood must be done in the fume hood!
8. Report all safety incidents to the instructor!
9. Write your raw data directly into your lab notebook!
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